Restriction map for
pF1KB5258
(Circular) (Six-base) MAPSORT of: pF1KB5258.seq Check: 785 from: 1 to: 4085
pF1KB5258 4085 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 720 798 1123 720
Size: 78 325 3682
Fragments arranged by size:
3682 325 78
Acc65I G'GTAC_C
Cuts at: 947 1101 947
Size: 154 3931
AclI AA'CG_TT
Cuts at: 3925 3925
Size: 4085
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1910 2342 2695 3357 1910
Size: 432 353 662 2638
Fragments arranged by size:
2638 662 432 353
AflIII A'CryG_T
Cuts at: 1437 3237 3518 1437
Size: 1800 281 2004
Fragments arranged by size:
2004 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 923 923
Size: 4085
AlwNI CAG_nnn'CTG
Cuts at: 490 1390 2828 490
Size: 900 1438 1747
Fragments arranged by size:
1747 1438 900
ApaI G_GGCC'C
Cuts at: 853 853
Size: 4085
ApaLI G'TGCA_C
Cuts at: 2923 2923
Size: 4085
ApoI r'AATT_y
Cuts at: 1041 1089 3438 3694 1041
Size: 48 2349 256 1432
Fragments arranged by size:
2349 1432 256 48
AseI AT'TA_AT
Cuts at: 20 20
Size: 4085
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4085
AvaI C'yCGr_G
Cuts at: 222 1054 1105 222
Size: 832 51 3202
Fragments arranged by size:
3202 832 51
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 931 964 931
Size: 33 4052
BamHI G'GATC_C
Cuts at: 1110 1110
Size: 4085
BanI G'GyrC_C
Cuts at: 243 947 1101 1515 1763 1798 243
Size: 704 154 414 248 35 2530
Fragments arranged by size:
2530 704 414 248 154 35
BanII G_rGCy'C
Cuts at: 853 1099 2129 853
Size: 246 1030 2809
Fragments arranged by size:
2809 1030 246
BbeI G_GCGC'C
Cuts at: 247 1519 1767 247
Size: 1272 248 2565
Fragments arranged by size:
2565 1272 248
BbvCI CC'TCA_GC
Cuts at: 166 1076 166
Size: 910 3175
BciVI GTATCCnnnnn_n'
Cuts at: 1983 3039 4085 1983
Size: 1056 1046 1983
Fragments arranged by size:
1983 1056 1046
BclI T'GATC_A
Cuts at: 1605 1605
Size: 4085
BglI GCCn_nnn'nGGC
Cuts at: 227 227
Size: 4085
BglII A'GATC_T
Cuts at: 1600 3637 1600
Size: 2037 2048
BlpI GC'TnA_GC
Cuts at: 1061 1197 1061
Size: 136 3949
Bme1580I G_kGCm'C
Cuts at: 853 1710 1803 2927 853
Size: 857 93 1124 2011
Fragments arranged by size:
2011 1124 857 93
BmgBI CAC'GTC
Cuts at: 556 556
Size: 4085
BmrI ACTGGGnnnn_n'
Cuts at: 1297 1378 1713 3857 1297
Size: 81 335 2144 1525
Fragments arranged by size:
2144 1525 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2452 2452
Size: 4085
Bpu10I CC'TnA_GC
Cuts at: 166 1076 166
Size: 910 3175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1261 2140 2854 3152 1261
Size: 879 714 298 2194
Fragments arranged by size:
2194 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 118 699 910 3789 118
Size: 581 211 2879 414
Fragments arranged by size:
2879 581 414 211
BsaAI yAC'GTr
Cuts at: 1438 1438
Size: 4085
BsaBI GATnn'nnATC
Cuts at: 6 1599 3636 6
Size: 1593 2037 455
Fragments arranged by size:
2037 1593 455
BsaHI Gr'CG_yC
Cuts at: 244 717 978 1516 1764 2466 3962 244
Size: 473 261 538 248 702 1496 367
Fragments arranged by size:
1496 702 538 473 367 261 248
BsaWI w'CCGG_w
Cuts at: 1274 1795 2473 2884 3031 3305 1274
Size: 521 678 411 147 274 2054
Fragments arranged by size:
2054 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 416 446 416
Size: 30 4055
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 845 845
Size: 4085
BseYI C'CCAG_C
Cuts at: 128 1510 2268 2933 3341 128
Size: 1382 758 665 408 872
Fragments arranged by size:
1382 872 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 803 766
Size: 37 4048
BsiEI CG_ry'CG
Cuts at: 104 1627 1673 2903 104
Size: 1523 46 1230 1286
Fragments arranged by size:
1523 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 639 1099 1877 2067 2927 639
Size: 460 778 190 860 1797
Fragments arranged by size:
1797 860 778 460 190
Bsp1286I G_dGCh'C
Cuts at: 639 853 1099 1710 1803 1877 2067 2129 2927
Size: 214 246 611 93 74 190 62 798
Cuts at: 2927 639
Size: 1797
Fragments arranged by size:
1797 798 611 246 214 190 93 74
62
BspHI T'CATG_A
Cuts at: 2517 2517
Size: 4085
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1135 1349 1651 2032 1135
Size: 214 302 381 3188
Fragments arranged by size:
3188 381 302 214
BsrBI CCG'CTC
Cuts at: 50 215 1051 2377 2431 3915 4080 50
Size: 165 836 1326 54 1484 165 55
Fragments arranged by size:
1484 1326 836 165 165 55 54
BsrDI GCAATG_nn'
Cuts at: 891 1997 891
Size: 1106 2979
BsrFI r'CCGG_y
Cuts at: 250 662 2083 2264 250
Size: 412 1421 181 2071
Fragments arranged by size:
2071 1421 412 181
BsrGI T'GTAC_A
Cuts at: 363 363
Size: 4085
BssSI C'ACGA_G
Cuts at: 2356 3064 2356
Size: 708 3377
BstAPI GCAn_nnn'nTGC
Cuts at: 1462 1462
Size: 4085
BstBI TT'CG_AA
Cuts at: 2446 2446
Size: 4085
BstYI r'GATC_y
Cuts at: 1 1110 1577 1600 1935 2181 2487 2499 2585
Size: 1109 467 23 335 246 306 12 86
Cuts at: 2585 3637 1
Size: 1052 449
Fragments arranged by size:
1109 1052 467 449 335 306 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 255 2035 2216 2379 255
Size: 1780 181 163 1961
Fragments arranged by size:
1961 1780 181 163
BtsI GCAGTG_nn'
Cuts at: 417 1328 417
Size: 911 3174
DraI TTT'AAA
Cuts at: 1084 1084
Size: 4085
DrdI GACnn_nn'nnGTC
Cuts at: 265 1791 3135 265
Size: 1526 1344 1215
Fragments arranged by size:
1526 1344 1215
EaeI y'GGCC_r
Cuts at: 1670 1844 2235 2262 3607 1670
Size: 174 391 27 1345 2148
Fragments arranged by size:
2148 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1670 1670
Size: 4085
EarI CTCTTCn'nnn_
Cuts at: 2108 2318 2108
Size: 210 3875
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2293 3035 3181 3895 2293
Size: 742 146 714 2483
Fragments arranged by size:
2483 742 714 146
EcoICRI GAG'CTC
Cuts at: 1097 1097
Size: 4085
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1910 2342 2695 3357 1910
Size: 432 353 662 2638
Fragments arranged by size:
2638 662 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 672 672
Size: 4085
EcoO109I rG'GnC_Cy
Cuts at: 487 565 849 850 1224 487
Size: 78 284 1 374 3348
Fragments arranged by size:
3348 374 284 78 1
EcoRI G'AATT_C
Cuts at: 1041 1089 1041
Size: 48 4037
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2104 2104
Size: 4085
FspI TGC'GCA
Cuts at: 1866 1866
Size: 4085
HaeII r_GCGC'y
Cuts at: 173 247 1519 1767 2997 3768 173
Size: 74 1272 248 1230 771 490
Fragments arranged by size:
1272 1230 771 490 248 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 909 941 1030 1062 1927 1957 1959 1989 909
Size: 32 89 32 865 30 2 30 3005
Fragments arranged by size:
3005 865 89 32 32 30 30 2
HincII GTy'rAC
Cuts at: 721 1124 721
Size: 403 3682
KasI G'GCGC_C
Cuts at: 243 1515 1763 243
Size: 1272 248 2565
Fragments arranged by size:
2565 1272 248
KpnI G_GTAC'C
Cuts at: 951 1105 951
Size: 154 3931
MluI A'CGCG_T
Cuts at: 3518 3518
Size: 4085
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 758 2844 3028 758
Size: 2086 184 1815
Fragments arranged by size:
2086 1815 184
MscI TGG'CCA
Cuts at: 1846 1846
Size: 4085
MslI CAynn'nnrTG
Cuts at: 2074 2201 2074
Size: 127 3958
MspA1I CmG'CkG
Cuts at: 203 1036 1196 1510 1870 2654 2899 203
Size: 833 160 314 360 784 245 1389
Fragments arranged by size:
1389 833 784 360 314 245 160
NaeI GCC'GGC
Cuts at: 252 664 2266 252
Size: 412 1602 2071
Fragments arranged by size:
2071 1602 412
NarI GG'CG_CC
Cuts at: 244 1516 1764 244
Size: 1272 248 2565
Fragments arranged by size:
2565 1272 248
NgoMIV G'CCGG_C
Cuts at: 250 662 2264 250
Size: 412 1602 2071
Fragments arranged by size:
2071 1602 412
NspI r_CATG'y
Cuts at: 702 1138 1638 3241 702
Size: 436 500 1603 1546
Fragments arranged by size:
1603 1546 500 436
PciI A'CATG_T
Cuts at: 3237 3237
Size: 4085
PflMI CCAn_nnn'nTGG
Cuts at: 1003 1003
Size: 4085
PmeI GTTT'AAAC
Cuts at: 1084 1084
Size: 4085
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2531 2563 2531
Size: 32 4053
PpuMI rG'GwC_Cy
Cuts at: 487 565 487
Size: 78 4007
PspOMI G'GGCC_C
Cuts at: 849 849
Size: 4085
PstI C_TGCA'G
Cuts at: 1132 1817 1132
Size: 685 3400
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4085
PvuII CAG'CTG
Cuts at: 1036 1510 1870 1036
Size: 474 360 3251
Fragments arranged by size:
3251 474 360
RsrII CG'GwC_CG
Cuts at: 2280 2280
Size: 4085
SacI G_AGCT'C
Cuts at: 1099 1099
Size: 4085
SalI G'TCGA_C
Cuts at: 719 1122 719
Size: 403 3682
SapI GCTCTTCn'nnn_
Cuts at: 2108 2318 2108
Size: 210 3875
SbfI CC_TGCA'GG
Cuts at: 1132 1132
Size: 4085
ScaI AGT'ACT
Cuts at: 3602 3602
Size: 4085
SfcI C'TryA_G
Cuts at: 33 1128 1813 2781 2972 33
Size: 1095 685 968 191 1146
Fragments arranged by size:
1146 1095 968 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 227 227
Size: 4085
SfoI GGC'GCC
Cuts at: 245 1517 1765 245
Size: 1272 248 2565
Fragments arranged by size:
2565 1272 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4085
SmaI CCC'GGG
Cuts at: 224 1107 224
Size: 883 3202
SmlI C'TyrA_G
Cuts at: 1240 2155 2869 3131 1240
Size: 915 714 262 2194
Fragments arranged by size:
2194 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4085
SphI G_CATG'C
Cuts at: 1138 1638 1138
Size: 500 3585
SrfI GCCC'GGGC
Cuts at: 224 224
Size: 4085
StyI C'CwwG_G
Cuts at: 347 921 1219 1573 347
Size: 574 298 354 2859
Fragments arranged by size:
2859 574 354 298
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1668 1801 2260 2469 1668
Size: 133 459 209 3284
Fragments arranged by size:
3284 459 209 133
TatI w'GTAC_w
Cuts at: 363 3600 363
Size: 3237 848
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 416 448 3602 3634 416
Size: 32 3154 32 867
Fragments arranged by size:
3154 867 32 32
Tth111I GACn'n_nGTC
Cuts at: 1882 1882
Size: 4085
XbaI T'CTAG_A
Cuts at: 1116 1116
Size: 4085
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1580 1580
Size: 4085
XmaI C'CCGG_G
Cuts at: 222 1105 222
Size: 883 3202
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AlfI AloI
AscI AvrII BbsI BcgI BfrBI BmtI BpmI BsiWI
BsmI BsmBI BspEI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SgrAI SnaBI SspI StuI SwaI
XhoI XmnI ZraI