Restriction map for
pF1KB5251
(Circular) (Six-base) MAPSORT of: pF1KB5251.seq Check: 5438 from: 1 to: 3938
pF1KB5251 3938 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 669 669
Size: 3938
AccI GT'mk_AC
Cuts at: 227 976 227
Size: 749 3189
Acc65I G'GTAC_C
Cuts at: 954 954
Size: 3938
AclI AA'CG_TT
Cuts at: 3778 3778
Size: 3938
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 742 1763 2195 2942 3210 368
Size: 374 1021 432 747 268 1096
Fragments arranged by size:
1096 1021 747 432 374 268
AfeI AGC'GCT
Cuts at: 185 185
Size: 3938
AflIII A'CryG_T
Cuts at: 230 1290 2394 3371 230
Size: 1060 1104 977 797
Fragments arranged by size:
1104 1060 977 797
AgeI A'CCGG_T
Cuts at: 2326 2326
Size: 3938
AlwNI CAG_nnn'CTG
Cuts at: 210 714 847 1243 2810 210
Size: 504 133 396 1567 1338
Fragments arranged by size:
1567 1338 504 396 133
ApaLI G'TGCA_C
Cuts at: 2708 2708
Size: 3938
ApoI r'AATT_y
Cuts at: 942 3291 3547 942
Size: 2349 256 1333
Fragments arranged by size:
2349 1333 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3938
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3938
AvaI C'yCGr_G
Cuts at: 562 958 562
Size: 396 3542
BamHI G'GATC_C
Cuts at: 963 963
Size: 3938
BanI G'GyrC_C
Cuts at: 850 954 1368 1616 1651 850
Size: 104 414 248 35 3137
Fragments arranged by size:
3137 414 248 104 35
BanII G_rGCy'C
Cuts at: 899 952 1982 899
Size: 53 1030 2855
Fragments arranged by size:
2855 1030 53
BbeI G_GCGC'C
Cuts at: 1372 1620 1372
Size: 248 3690
BbsI GAAGACnn'nnnn_
Cuts at: 500 500
Size: 3938
BbvCI CC'TCA_GC
Cuts at: 832 832
Size: 3938
BciVI GTATCCnnnnn_n'
Cuts at: 448 1836 2597 3938 448
Size: 1388 761 1341 448
Fragments arranged by size:
1388 1341 761 448
BclI T'GATC_A
Cuts at: 1458 1458
Size: 3938
BglI GCCn_nnn'nGGC
Cuts at: 169 178 169
Size: 9 3929
BglII A'GATC_T
Cuts at: 1453 3490 1453
Size: 2037 1901
BlpI GC'TnA_GC
Cuts at: 1050 1050
Size: 3938
Bme1580I G_kGCm'C
Cuts at: 1563 1656 2712 1563
Size: 93 1056 2789
Fragments arranged by size:
2789 1056 93
BmgBI CAC'GTC
Cuts at: 525 525
Size: 3938
BmrI ACTGGGnnnn_n'
Cuts at: 1150 1231 1566 3710 1150
Size: 81 335 2144 1378
Fragments arranged by size:
2144 1378 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2305 2305
Size: 3938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 478 232
Size: 246 3692
Bpu10I CC'TnA_GC
Cuts at: 812 832 812
Size: 20 3918
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1114 1993 2485 2783 1114
Size: 879 492 298 2269
Fragments arranged by size:
2269 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3642 3642
Size: 3938
BsaAI yAC'GTr
Cuts at: 231 1291 231
Size: 1060 2878
BsaBI GATnn'nnATC
Cuts at: 6 1452 3489 6
Size: 1446 2037 455
Fragments arranged by size:
2037 1446 455
BsaHI Gr'CG_yC
Cuts at: 1369 1617 2319 3815 1369
Size: 248 702 1496 1492
Fragments arranged by size:
1496 1492 702 248
BsaWI w'CCGG_w
Cuts at: 1127 1648 2326 2600 2747 3158 1127
Size: 521 678 274 147 411 1907
Fragments arranged by size:
1907 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 688 688
Size: 3938
BseYI C'CCAG_C
Cuts at: 714 868 1363 2121 2698 3194 714
Size: 154 495 758 577 496 1458
Fragments arranged by size:
1458 758 577 496 495 154
BsiEI CG_ry'CG
Cuts at: 104 1480 1526 2734 104
Size: 1376 46 1208 1308
Fragments arranged by size:
1376 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 952 1730 1920 2712 952
Size: 778 190 792 2178
Fragments arranged by size:
2178 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 899 952 1563 1656 1730 1920 1982 2712 899
Size: 53 611 93 74 190 62 730 2125
Fragments arranged by size:
2125 730 611 190 93 74 62 53
BspEI T'CCGG_A
Cuts at: 3158 3158
Size: 3938
BspHI T'CATG_A
Cuts at: 3114 3114
Size: 3938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 669 988 1202 1504 1885 669
Size: 319 214 302 381 2722
Fragments arranged by size:
2722 381 319 302 214
BsrBI CCG'CTC
Cuts at: 50 2230 2284 3768 3933 50
Size: 2180 54 1484 165 55
Fragments arranged by size:
2180 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 614 1850 614
Size: 1236 2702
BsrFI r'CCGG_y
Cuts at: 1936 2117 2326 1936
Size: 181 209 3548
Fragments arranged by size:
3548 209 181
BssSI C'ACGA_G
Cuts at: 2209 2567 2209
Size: 358 3580
BstAPI GCAn_nnn'nTGC
Cuts at: 153 1315 153
Size: 1162 2776
BstBI TT'CG_AA
Cuts at: 2299 2299
Size: 3938
BstYI r'GATC_y
Cuts at: 1 963 1430 1453 1788 2034 3046 3132 3144
Size: 962 467 23 335 246 1012 86 12
Cuts at: 3144 3490 1
Size: 346 449
Fragments arranged by size:
1012 962 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1888 2069 2232 1888
Size: 181 163 3594
Fragments arranged by size:
3594 181 163
BtsI GCAGTG_nn'
Cuts at: 455 1181 455
Size: 726 3212
DraI TTT'AAA
Cuts at: 937 937
Size: 3938
DrdI GACnn_nn'nnGTC
Cuts at: 1644 2502 1644
Size: 858 3080
EaeI y'GGCC_r
Cuts at: 170 188 1523 1697 2088 2115 3460 170
Size: 18 1335 174 391 27 1345 648
Fragments arranged by size:
1345 1335 648 391 174 27 18
EagI C'GGCC_G
Cuts at: 1523 1523
Size: 3938
EarI CTCTTCn'nnn_
Cuts at: 1961 2171 1961
Size: 210 3728
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2146 2456 2602 3748 2146
Size: 310 146 1146 2336
Fragments arranged by size:
2336 1146 310 146
EcoICRI GAG'CTC
Cuts at: 950 950
Size: 3938
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 368 478 742 1763 2195 2942 3210 232
Size: 136 110 264 1021 432 747 268 960
Fragments arranged by size:
1021 960 747 432 268 264 136 110
EcoO109I rG'GnC_Cy
Cuts at: 216 603 807 1077 216
Size: 387 204 270 3077
Fragments arranged by size:
3077 387 270 204
EcoRI G'AATT_C
Cuts at: 942 942
Size: 3938
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1957 1957
Size: 3938
FspI TGC'GCA
Cuts at: 1719 1719
Size: 3938
HaeII r_GCGC'y
Cuts at: 187 600 864 911 1372 1620 2642 3621 187
Size: 413 264 47 461 248 1022 979 504
Fragments arranged by size:
1022 979 504 461 413 264 248 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 274 306 1780 1810 1812 1842 274
Size: 32 1474 30 2 30 2370
Fragments arranged by size:
2370 1474 32 30 30 2
HincII GTy'rAC
Cuts at: 570 977 570
Size: 407 3531
HindIII A'AGCT_T
Cuts at: 391 391
Size: 3938
KasI G'GCGC_C
Cuts at: 1368 1616 1368
Size: 248 3690
KpnI G_GTAC'C
Cuts at: 958 958
Size: 3938
MluI A'CGCG_T
Cuts at: 3371 3371
Size: 3938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2609 2793 2609
Size: 184 3754
MscI TGG'CCA
Cuts at: 190 1699 190
Size: 1509 2429
MslI CAynn'nnrTG
Cuts at: 1927 2054 1927
Size: 127 3811
MspA1I CmG'CkG
Cuts at: 489 558 714 1049 1363 1723 2736 2981 489
Size: 69 156 335 314 360 1013 245 1446
Fragments arranged by size:
1446 1013 360 335 314 245 156 69
NaeI GCC'GGC
Cuts at: 2119 2119
Size: 3938
NarI GG'CG_CC
Cuts at: 1369 1617 1369
Size: 248 3690
NdeI CA'TA_TG
Cuts at: 444 444
Size: 3938
NgoMIV G'CCGG_C
Cuts at: 2117 2117
Size: 3938
NspI r_CATG'y
Cuts at: 255 991 1491 2398 255
Size: 736 500 907 1795
Fragments arranged by size:
1795 907 736 500
PciI A'CATG_T
Cuts at: 2394 2394
Size: 3938
PflMI CCAn_nnn'nTGG
Cuts at: 847 917 847
Size: 70 3868
PmeI GTTT'AAAC
Cuts at: 937 937
Size: 3938
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3077 3109 3077
Size: 32 3906
PpuMI rG'GwC_Cy
Cuts at: 603 807 603
Size: 204 3734
PspXI vC'TCGA_Gb
Cuts at: 562 562
Size: 3938
PstI C_TGCA'G
Cuts at: 632 985 1670 632
Size: 353 685 2900
Fragments arranged by size:
2900 685 353
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3938
PvuII CAG'CTG
Cuts at: 489 714 1363 1723 489
Size: 225 649 360 2704
Fragments arranged by size:
2704 649 360 225
RsrII CG'GwC_CG
Cuts at: 2133 2133
Size: 3938
SacI G_AGCT'C
Cuts at: 952 952
Size: 3938
SalI G'TCGA_C
Cuts at: 975 975
Size: 3938
SapI GCTCTTCn'nnn_
Cuts at: 1961 2171 1961
Size: 210 3728
SbfI CC_TGCA'GG
Cuts at: 632 985 632
Size: 353 3585
ScaI AGT'ACT
Cuts at: 3455 3455
Size: 3938
SfcI C'TryA_G
Cuts at: 33 573 628 981 1666 2659 2850 33
Size: 540 55 353 685 993 191 1121
Fragments arranged by size:
1121 993 685 540 353 191 55
SfoI GGC'GCC
Cuts at: 1370 1618 1370
Size: 248 3690
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3938
SmaI CCC'GGG
Cuts at: 960 960
Size: 3938
SmlI C'TyrA_G
Cuts at: 562 1093 2008 2500 2762 562
Size: 531 915 492 262 1738
Fragments arranged by size:
1738 915 531 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3938
SphI G_CATG'C
Cuts at: 991 1491 991
Size: 500 3438
StyI C'CwwG_G
Cuts at: 173 1072 1426 173
Size: 899 354 2685
Fragments arranged by size:
2685 899 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1521 1654 2113 2322 1521
Size: 133 459 209 3137
Fragments arranged by size:
3137 459 209 133
TatI w'GTAC_w
Cuts at: 3453 3453
Size: 3938
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3455 3487 3455
Size: 32 3906
Tth111I GACn'n_nGTC
Cuts at: 1735 1735
Size: 3938
XbaI T'CTAG_A
Cuts at: 969 969
Size: 3938
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1433 1433
Size: 3938
XhoI C'TCGA_G
Cuts at: 562 562
Size: 3938
XmaI C'CCGG_G
Cuts at: 958 958
Size: 3938
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmtI BsaXI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NcoI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PshAI PsiI PspOMI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XmnI ZraI