Restriction map for
pF1KB5238
(Circular) (Six-base) MAPSORT of: pF1KB5238.seq Check: 6405 from: 1 to: 3734
pF1KB5238 3734 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AatII G_ACGT'C
Cuts at: 647 647
Size: 3734
AccI GT'mk_AC
Cuts at: 772 772
Size: 3734
Acc65I G'GTAC_C
Cuts at: 750 750
Size: 3734
AclI AA'CG_TT
Cuts at: 3574 3574
Size: 3734
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 324 325 523 1559 1991 2738 3006 324
Size: 1 198 1036 432 747 268 1052
Fragments arranged by size:
1052 1036 747 432 268 198 1
AflIII A'CryG_T
Cuts at: 1086 2190 3167 1086
Size: 1104 977 1653
Fragments arranged by size:
1653 1104 977
AgeI A'CCGG_T
Cuts at: 2122 2122
Size: 3734
AlwNI CAG_nnn'CTG
Cuts at: 1039 2606 1039
Size: 1567 2167
ApaLI G'TGCA_C
Cuts at: 351 2504 351
Size: 2153 1581
ApoI r'AATT_y
Cuts at: 738 3087 3343 738
Size: 2349 256 1129
Fragments arranged by size:
2349 1129 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3734
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3734
AvaI C'yCGr_G
Cuts at: 754 754
Size: 3734
BamHI G'GATC_C
Cuts at: 759 759
Size: 3734
BanI G'GyrC_C
Cuts at: 750 1164 1412 1447 750
Size: 414 248 35 3037
Fragments arranged by size:
3037 414 248 35
BanII G_rGCy'C
Cuts at: 208 348 748 1778 208
Size: 140 400 1030 2164
Fragments arranged by size:
2164 1030 400 140
BbeI G_GCGC'C
Cuts at: 1168 1416 1168
Size: 248 3486
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 627 661 90
Size: 34 503 34 3163
Fragments arranged by size:
3163 503 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1632 2393 3734 1632
Size: 761 1341 1632
Fragments arranged by size:
1632 1341 761
BclI T'GATC_A
Cuts at: 1254 1254
Size: 3734
BglI GCCn_nnn'nGGC
Cuts at: 112 425 112
Size: 313 3421
BglII A'GATC_T
Cuts at: 1249 3286 1249
Size: 2037 1697
BlpI GC'TnA_GC
Cuts at: 846 846
Size: 3734
Bme1580I G_kGCm'C
Cuts at: 355 1359 1452 2508 355
Size: 1004 93 1056 1581
Fragments arranged by size:
1581 1056 1004 93
BmgBI CAC'GTC
Cuts at: 261 627 261
Size: 366 3368
BmrI ACTGGGnnnn_n'
Cuts at: 558 946 1027 1362 3506 558
Size: 388 81 335 2144 786
Fragments arranged by size:
2144 786 388 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 3734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 319
Size: 3734
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 1789 2281 2579 910
Size: 879 492 298 2065
Fragments arranged by size:
2065 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3438 3438
Size: 3734
BsaAI yAC'GTr
Cuts at: 510 1087 510
Size: 577 3157
BsaBI GATnn'nnATC
Cuts at: 6 703 1248 3285 6
Size: 697 545 2037 455
Fragments arranged by size:
2037 697 545 455
BsaHI Gr'CG_yC
Cuts at: 266 644 1165 1413 2115 3611 266
Size: 378 521 248 702 1496 389
Fragments arranged by size:
1496 702 521 389 378 248
BsaWI w'CCGG_w
Cuts at: 923 1444 2122 2396 2543 2954 923
Size: 521 678 274 147 411 1703
Fragments arranged by size:
1703 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 367 397 367
Size: 30 3704
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 219 222 219
Size: 3 3731
BseYI C'CCAG_C
Cuts at: 1159 1917 2494 2990 1159
Size: 758 577 496 1903
Fragments arranged by size:
1903 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 561 561
Size: 3734
BsiEI CG_ry'CG
Cuts at: 104 288 1276 1322 2530 104
Size: 184 988 46 1208 1308
Fragments arranged by size:
1308 1208 988 184 46
BsiHKAI G_wGCw'C
Cuts at: 355 748 1526 1716 2508 355
Size: 393 778 190 792 1581
Fragments arranged by size:
1581 792 778 393 190
Bsp1286I G_dGCh'C
Cuts at: 208 348 355 748 1359 1452 1526 1716 1778
Size: 140 7 393 611 93 74 190 62
Cuts at: 1778 2508 208
Size: 730 1434
Fragments arranged by size:
1434 730 611 393 190 140 93 74
62 7
BspEI T'CCGG_A
Cuts at: 2954 2954
Size: 3734
BspHI T'CATG_A
Cuts at: 2910 2910
Size: 3734
BspMI ACCTGCnnnn'nnnn_
Cuts at: 784 998 1300 1681 784
Size: 214 302 381 2837
Fragments arranged by size:
2837 381 302 214
BsrBI CCG'CTC
Cuts at: 50 246 282 2026 2080 3564 3729 50
Size: 196 36 1744 54 1484 165 55
Fragments arranged by size:
1744 1484 196 165 55 54 36
BsrDI GCAATG_nn'
Cuts at: 1646 1646
Size: 3734
BsrFI r'CCGG_y
Cuts at: 1732 1913 2122 1732
Size: 181 209 3344
Fragments arranged by size:
3344 209 181
BssSI C'ACGA_G
Cuts at: 247 2005 2363 247
Size: 1758 358 1618
Fragments arranged by size:
1758 1618 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1111 1111
Size: 3734
BstBI TT'CG_AA
Cuts at: 2095 2095
Size: 3734
BstYI r'GATC_y
Cuts at: 1 759 1226 1249 1584 1830 2842 2928 2940
Size: 758 467 23 335 246 1012 86 12
Cuts at: 2940 3286 1
Size: 346 449
Fragments arranged by size:
1012 758 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 182 530 182
Size: 348 3386
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1684 1865 2028 1684
Size: 181 163 3390
Fragments arranged by size:
3390 181 163
BtsI GCAGTG_nn'
Cuts at: 977 977
Size: 3734
DraI TTT'AAA
Cuts at: 733 733
Size: 3734
DraIII CAC_nnn'GTG
Cuts at: 399 399
Size: 3734
DrdI GACnn_nn'nnGTC
Cuts at: 1440 2298 1440
Size: 858 2876
EaeI y'GGCC_r
Cuts at: 285 717 1319 1493 1884 1911 3256 285
Size: 432 602 174 391 27 1345 763
Fragments arranged by size:
1345 763 602 432 391 174 27
EagI C'GGCC_G
Cuts at: 285 1319 285
Size: 1034 2700
EarI CTCTTCn'nnn_
Cuts at: 228 1757 1967 228
Size: 1529 210 1995
Fragments arranged by size:
1995 1529 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1942 2252 2398 3544 1942
Size: 310 146 1146 2132
Fragments arranged by size:
2132 1146 310 146
EcoICRI GAG'CTC
Cuts at: 746 746
Size: 3734
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 324 325 523 1559 1991 2738 3006 319
Size: 5 1 198 1036 432 747 268 1047
Fragments arranged by size:
1047 1036 747 432 268 198 5 1
EcoNI CCTnn'n_nnAGG
Cuts at: 393 393
Size: 3734
EcoO109I rG'GnC_Cy
Cuts at: 873 873
Size: 3734
EcoRI G'AATT_C
Cuts at: 738 738
Size: 3734
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1753 1753
Size: 3734
FspI TGC'GCA
Cuts at: 1515 1515
Size: 3734
HaeII r_GCGC'y
Cuts at: 118 1168 1416 2438 3417 118
Size: 1050 248 1022 979 435
Fragments arranged by size:
1050 1022 979 435 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 205 237 1576 1606 1608 1638 205
Size: 32 1339 30 2 30 2301
Fragments arranged by size:
2301 1339 32 30 30 2
HincII GTy'rAC
Cuts at: 773 773
Size: 3734
KasI G'GCGC_C
Cuts at: 1164 1412 1164
Size: 248 3486
KpnI G_GTAC'C
Cuts at: 754 754
Size: 3734
MluI A'CGCG_T
Cuts at: 3167 3167
Size: 3734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 578 2405 2589 578
Size: 1827 184 1723
Fragments arranged by size:
1827 1723 184
MscI TGG'CCA
Cuts at: 719 1495 719
Size: 776 2958
MslI CAynn'nnrTG
Cuts at: 1723 1850 1723
Size: 127 3607
MspA1I CmG'CkG
Cuts at: 134 171 184 331 845 1159 1519 2532 2777
Size: 37 13 147 514 314 360 1013 245
Cuts at: 2777 134
Size: 1091
Fragments arranged by size:
1091 1013 514 360 314 245 147 37
13
NaeI GCC'GGC
Cuts at: 1915 1915
Size: 3734
NarI GG'CG_CC
Cuts at: 1165 1413 1165
Size: 248 3486
NcoI C'CATG_G
Cuts at: 530 530
Size: 3734
NgoMIV G'CCGG_C
Cuts at: 1913 1913
Size: 3734
NspI r_CATG'y
Cuts at: 613 787 1287 2194 613
Size: 174 500 907 2153
Fragments arranged by size:
2153 907 500 174
PciI A'CATG_T
Cuts at: 2190 2190
Size: 3734
PfoI T'CCnGG_A
Cuts at: 235 235
Size: 3734
PmeI GTTT'AAAC
Cuts at: 733 733
Size: 3734
PmlI CAC'GTG
Cuts at: 510 510
Size: 3734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2873 2905 2873
Size: 32 3702
PstI C_TGCA'G
Cuts at: 199 781 1466 199
Size: 582 685 2467
Fragments arranged by size:
2467 685 582
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3734
PvuII CAG'CTG
Cuts at: 331 1159 1519 331
Size: 828 360 2546
Fragments arranged by size:
2546 828 360
RsrII CG'GwC_CG
Cuts at: 1929 1929
Size: 3734
SacI G_AGCT'C
Cuts at: 748 748
Size: 3734
SacII CC_GC'GG
Cuts at: 185 185
Size: 3734
SalI G'TCGA_C
Cuts at: 771 771
Size: 3734
SapI GCTCTTCn'nnn_
Cuts at: 1757 1967 1757
Size: 210 3524
SbfI CC_TGCA'GG
Cuts at: 781 781
Size: 3734
ScaI AGT'ACT
Cuts at: 3251 3251
Size: 3734
SfcI C'TryA_G
Cuts at: 33 195 777 1462 2455 2646 33
Size: 162 582 685 993 191 1121
Fragments arranged by size:
1121 993 685 582 191 162
SfoI GGC'GCC
Cuts at: 1166 1414 1166
Size: 248 3486
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3734
SmaI CCC'GGG
Cuts at: 756 756
Size: 3734
SmlI C'TyrA_G
Cuts at: 889 1804 2296 2558 889
Size: 915 492 262 2065
Fragments arranged by size:
2065 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3734
SphI G_CATG'C
Cuts at: 787 1287 787
Size: 500 3234
StyI C'CwwG_G
Cuts at: 530 868 1222 530
Size: 338 354 3042
Fragments arranged by size:
3042 354 338
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 610 1317 1450 1909 2118 610
Size: 707 133 459 209 2226
Fragments arranged by size:
2226 707 459 209 133
TatI w'GTAC_w
Cuts at: 3249 3249
Size: 3734
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3251 3283 3251
Size: 32 3702
Tth111I GACn'n_nGTC
Cuts at: 1531 1531
Size: 3734
XbaI T'CTAG_A
Cuts at: 765 765
Size: 3734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1229 1229
Size: 3734
XmaI C'CCGG_G
Cuts at: 754 754
Size: 3734
XmnI GAAnn'nnTTC
Cuts at: 367 367
Size: 3734
ZraI GAC'GTC
Cuts at: 645 645
Size: 3734
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PmlI PpiI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BmtI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI