Restriction map for
pF1KB5235
(Circular) (Six-base) MAPSORT of: pF1KB5235.seq Check: 4060 from: 1 to: 4640
pF1KB5235 4640 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 821 1678 821
Size: 857 3783
Acc65I G'GTAC_C
Cuts at: 1656 1656
Size: 4640
AclI AA'CG_TT
Cuts at: 4480 4480
Size: 4640
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 250 478 697 703 1033 2465 2897 3250
Size: 120 228 219 6 330 1432 432 353
Cuts at: 3250 3912 130
Size: 662 858
Fragments arranged by size:
1432 858 662 432 353 330 228 219
120 6
AflIII A'CryG_T
Cuts at: 1992 3792 4073 1992
Size: 1800 281 2559
Fragments arranged by size:
2559 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 837 837
Size: 4640
AleI CACnn'nnGTG
Cuts at: 1105 1105
Size: 4640
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 941 973 941
Size: 32 4608
AlwNI CAG_nnn'CTG
Cuts at: 1945 3383 1945
Size: 1438 3202
ApaLI G'TGCA_C
Cuts at: 3478 3478
Size: 4640
ApoI r'AATT_y
Cuts at: 265 616 1644 3993 4249 265
Size: 351 1028 2349 256 656
Fragments arranged by size:
2349 1028 656 351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4640
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4640
AvaI C'yCGr_G
Cuts at: 1082 1660 1082
Size: 578 4062
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 377 410 377
Size: 33 4607
BamHI G'GATC_C
Cuts at: 963 1665 963
Size: 702 3938
BanI G'GyrC_C
Cuts at: 1236 1656 2070 2318 2353 1236
Size: 420 414 248 35 3523
Fragments arranged by size:
3523 420 414 248 35
BanII G_rGCy'C
Cuts at: 479 1160 1253 1654 2684 479
Size: 681 93 401 1030 2435
Fragments arranged by size:
2435 1030 681 401 93
BbeI G_GCGC'C
Cuts at: 2074 2322 2074
Size: 248 4392
BbsI GAAGACnn'nnnn_
Cuts at: 440 1589 440
Size: 1149 3491
BciVI GTATCCnnnnn_n'
Cuts at: 948 2538 3594 4640 948
Size: 1590 1056 1046 948
Fragments arranged by size:
1590 1056 1046 948
BclI T'GATC_A
Cuts at: 2160 2160
Size: 4640
BglII A'GATC_T
Cuts at: 2155 4192 2155
Size: 2037 2603
BlpI GC'TnA_GC
Cuts at: 1252 1421 1752 1252
Size: 169 331 4140
Fragments arranged by size:
4140 331 169
Bme1580I G_kGCm'C
Cuts at: 292 2265 2358 3482 292
Size: 1973 93 1124 1450
Fragments arranged by size:
1973 1450 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1112 1852 1933 2268 4412 1112
Size: 740 81 335 2144 1340
Fragments arranged by size:
2144 1340 740 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1097 3007 1097
Size: 1910 2730
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 943 1205 148
Size: 795 262 3583
Fragments arranged by size:
3583 795 262
Bpu10I CC'TnA_GC
Cuts at: 1072 1072
Size: 4640
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 1504 1816 2695 3409 3707 154
Size: 1350 312 879 714 298 1087
Fragments arranged by size:
1350 1087 879 714 312 298
BsaI GGTCTCn'nnnn_
Cuts at: 1001 4344 1001
Size: 3343 1297
BsaAI yAC'GTr
Cuts at: 1993 1993
Size: 4640
BsaBI GATnn'nnATC
Cuts at: 6 2154 4191 6
Size: 2148 2037 455
Fragments arranged by size:
2148 2037 455
BsaHI Gr'CG_yC
Cuts at: 2071 2319 3021 4517 2071
Size: 248 702 1496 2194
Fragments arranged by size:
2194 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1829 2350 3028 3439 3586 3860 1829
Size: 521 678 411 147 274 2609
Fragments arranged by size:
2609 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 143 173 466 496 941 971 1045 1075 143
Size: 30 293 30 445 30 74 30 3708
Fragments arranged by size:
3708 445 293 74 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 145 217 220 804 1324 1489 145
Size: 72 3 584 520 165 3296
Fragments arranged by size:
3296 584 520 165 72 3
BseYI C'CCAG_C
Cuts at: 240 588 643 1137 1408 2065 2823 3488 3896
Size: 348 55 494 271 657 758 665 408
Cuts at: 3896 240
Size: 984
Fragments arranged by size:
984 758 665 657 494 408 348 271
55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 392 1337 392
Size: 945 3695
BsiEI CG_ry'CG
Cuts at: 104 747 2182 2228 3458 104
Size: 643 1435 46 1230 1286
Fragments arranged by size:
1435 1286 1230 643 46
BsiHKAI G_wGCw'C
Cuts at: 275 479 1654 2432 2622 3482 275
Size: 204 1175 778 190 860 1433
Fragments arranged by size:
1433 1175 860 778 204 190
BsmI GAATG_Cn'
Cuts at: 752 752
Size: 4640
Bsp1286I G_dGCh'C
Cuts at: 275 292 479 1160 1253 1654 2265 2358 2432
Size: 17 187 681 93 401 611 93 74
Cuts at: 2432 2622 2684 3482 275
Size: 190 62 798 1433
Fragments arranged by size:
1433 798 681 611 401 190 187 93
93 74 62 17
BspHI T'CATG_A
Cuts at: 3072 3072
Size: 4640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1690 1904 2206 2587 1690
Size: 214 302 381 3743
Fragments arranged by size:
3743 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1269 2932 2986 4470 4635 50
Size: 1219 1663 54 1484 165 55
Fragments arranged by size:
1663 1484 1219 165 55 54
BsrDI GCAATG_nn'
Cuts at: 533 638 2552 533
Size: 105 1914 2621
Fragments arranged by size:
2621 1914 105
BsrFI r'CCGG_y
Cuts at: 2638 2819 2638
Size: 181 4459
BsrGI T'GTAC_A
Cuts at: 1430 1430
Size: 4640
BssSI C'ACGA_G
Cuts at: 2911 3619 2911
Size: 708 3932
BstAPI GCAn_nnn'nTGC
Cuts at: 118 2017 118
Size: 1899 2741
BstBI TT'CG_AA
Cuts at: 3001 3001
Size: 4640
BstXI CCAn_nnnn'nTGG
Cuts at: 928 928
Size: 4640
BstYI r'GATC_y
Cuts at: 1 698 963 1665 2132 2155 2490 2736 3042
Size: 697 265 702 467 23 335 246 306
Cuts at: 3042 3054 3140 4192 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 702 697 467 449 335 306 265
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 890 890
Size: 4640
BtgI C'CryG_G
Cuts at: 107 366 600 1325 107
Size: 259 234 725 3422
Fragments arranged by size:
3422 725 259 234
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2590 2771 2934 2590
Size: 181 163 4296
Fragments arranged by size:
4296 181 163
BtsI GCAGTG_nn'
Cuts at: 1127 1179 1883 1127
Size: 52 704 3884
Fragments arranged by size:
3884 704 52
DraI TTT'AAA
Cuts at: 1639 1639
Size: 4640
DrdI GACnn_nn'nnGTC
Cuts at: 730 2346 3690 730
Size: 1616 1344 1680
Fragments arranged by size:
1680 1616 1344
EaeI y'GGCC_r
Cuts at: 603 2225 2399 2790 2817 4162 603
Size: 1622 174 391 27 1345 1081
Fragments arranged by size:
1622 1345 1081 391 174 27
EagI C'GGCC_G
Cuts at: 2225 2225
Size: 4640
EarI CTCTTCn'nnn_
Cuts at: 842 1148 1556 1572 2663 2873 842
Size: 306 408 16 1091 210 2609
Fragments arranged by size:
2609 1091 408 306 210 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2848 3590 3736 4450 2848
Size: 742 146 714 3038
Fragments arranged by size:
3038 742 714 146
EcoICRI GAG'CTC
Cuts at: 477 1652 477
Size: 1175 3465
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 148 250 478 697 703 943 1033 1205
Size: 18 102 228 219 6 240 90 172
Cuts at: 1205 2465 2897 3250 3912 130
Size: 1260 432 353 662 858
Fragments arranged by size:
1260 858 662 432 353 240 228 219
172 102 90 18 6
EcoNI CCTnn'n_nnAGG
Cuts at: 733 1591 733
Size: 858 3782
EcoO109I rG'GnC_Cy
Cuts at: 244 879 999 1779 244
Size: 635 120 780 3105
Fragments arranged by size:
3105 780 635 120
EcoRI G'AATT_C
Cuts at: 616 1644 616
Size: 1028 3612
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1008 1038 2659 1008
Size: 30 1621 2989
Fragments arranged by size:
2989 1621 30
FspI TGC'GCA
Cuts at: 2421 2421
Size: 4640
HaeII r_GCGC'y
Cuts at: 127 191 366 2074 2322 3552 4323 127
Size: 64 175 1708 248 1230 771 444
Fragments arranged by size:
1708 1230 771 444 248 175 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 466 498 823 855 1045 1077 2482 2512 2514
Size: 32 325 32 190 32 1405 30 2
Cuts at: 2514 2544 466
Size: 30 2562
Fragments arranged by size:
2562 1405 325 190 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1679 1679
Size: 4640
HindIII A'AGCT_T
Cuts at: 442 1384 442
Size: 942 3698
KasI G'GCGC_C
Cuts at: 2070 2318 2070
Size: 248 4392
KpnI G_GTAC'C
Cuts at: 1660 1660
Size: 4640
MluI A'CGCG_T
Cuts at: 4073 4073
Size: 4640
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3399 3583 3399
Size: 184 4456
MscI TGG'CCA
Cuts at: 2401 2401
Size: 4640
MslI CAynn'nnrTG
Cuts at: 1105 1261 2629 2756 1105
Size: 156 1368 127 2989
Fragments arranged by size:
2989 1368 156 127
MspA1I CmG'CkG
Cuts at: 240 954 1751 2065 2425 3209 3454 240
Size: 714 797 314 360 784 245 1426
Fragments arranged by size:
1426 797 784 714 360 314 245
NaeI GCC'GGC
Cuts at: 2821 2821
Size: 4640
NarI GG'CG_CC
Cuts at: 2071 2319 2071
Size: 248 4392
NcoI C'CATG_G
Cuts at: 107 107
Size: 4640
NgoMIV G'CCGG_C
Cuts at: 2819 2819
Size: 4640
NruI TCG'CGA
Cuts at: 326 326
Size: 4640
NspI r_CATG'y
Cuts at: 1215 1693 2193 3796 1215
Size: 478 500 1603 2059
Fragments arranged by size:
2059 1603 500 478
PasI CC'CwG_GG
Cuts at: 1161 1161
Size: 4640
PciI A'CATG_T
Cuts at: 3792 3792
Size: 4640
PfoI T'CCnGG_A
Cuts at: 959 959
Size: 4640
PmeI GTTT'AAAC
Cuts at: 1639 1639
Size: 4640
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 941 973 3086 3118 941
Size: 32 2113 32 2463
Fragments arranged by size:
2463 2113 32 32
PpuMI rG'GwC_Cy
Cuts at: 244 244
Size: 4640
PshAI GACnn'nnGTC
Cuts at: 302 302
Size: 4640
PspXI vC'TCGA_Gb
Cuts at: 1082 1082
Size: 4640
PstI C_TGCA'G
Cuts at: 1687 2372 1687
Size: 685 3955
PvuI CG_AT'CG
Cuts at: 104 747 104
Size: 643 3997
PvuII CAG'CTG
Cuts at: 240 954 2065 2425 240
Size: 714 1111 360 2455
Fragments arranged by size:
2455 1111 714 360
RsrII CG'GwC_CG
Cuts at: 2835 2835
Size: 4640
SacI G_AGCT'C
Cuts at: 479 1654 479
Size: 1175 3465
SalI G'TCGA_C
Cuts at: 1677 1677
Size: 4640
SapI GCTCTTCn'nnn_
Cuts at: 1148 2663 2873 1148
Size: 1515 210 2915
Fragments arranged by size:
2915 1515 210
SbfI CC_TGCA'GG
Cuts at: 1687 1687
Size: 4640
ScaI AGT'ACT
Cuts at: 1468 4157 1468
Size: 2689 1951
SfcI C'TryA_G
Cuts at: 33 300 1129 1683 2368 3336 3527 33
Size: 267 829 554 685 968 191 1146
Fragments arranged by size:
1146 968 829 685 554 267 191
SfoI GGC'GCC
Cuts at: 2072 2320 2072
Size: 248 4392
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4640
SmaI CCC'GGG
Cuts at: 1662 1662
Size: 4640
SmlI C'TyrA_G
Cuts at: 169 1082 1519 1795 2710 3424 3686 169
Size: 913 437 276 915 714 262 1123
Fragments arranged by size:
1123 915 913 714 437 276 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4640
SphI G_CATG'C
Cuts at: 1693 2193 1693
Size: 500 4140
StuI AGG'CCT
Cuts at: 167 991 167
Size: 824 3816
StyI C'CwwG_G
Cuts at: 107 284 992 1774 2128 107
Size: 177 708 782 354 2619
Fragments arranged by size:
2619 782 708 354 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1153 2223 2356 2815 3024 1153
Size: 1070 133 459 209 2769
Fragments arranged by size:
2769 1070 459 209 133
TatI w'GTAC_w
Cuts at: 1430 1466 4155 1430
Size: 36 2689 1915
Fragments arranged by size:
2689 1915 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 143 175 4157 4189 143
Size: 32 3982 32 594
Fragments arranged by size:
3982 594 32 32
Tth111I GACn'n_nGTC
Cuts at: 916 2437 916
Size: 1521 3119
XbaI T'CTAG_A
Cuts at: 756 1671 756
Size: 915 3725
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 928 2135 928
Size: 1207 3433
XhoI C'TCGA_G
Cuts at: 1082 1082
Size: 4640
XmaI C'CCGG_G
Cuts at: 1660 1660
Size: 4640
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PasI PciI PfoI PmeI PpiI PpuMI PshAI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AlfI ApaI AscI
AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI BsiWI
BsmBI BspEI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NsiI PacI PflMI PmlI PsiI PspOMI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XmnI ZraI