Restriction map for
pF1KB5232
(Circular) (Six-base) MAPSORT of: pF1KB5232.seq Check: 5806 from: 1 to: 4619
pF1KB5232 4619 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1298 1298
Size: 4619
AccI GT'mk_AC
Cuts at: 1657 1657
Size: 4619
Acc65I G'GTAC_C
Cuts at: 1283 1300 1635 1283
Size: 17 335 4267
Fragments arranged by size:
4267 335 17
AclI AA'CG_TT
Cuts at: 1244 4459 1244
Size: 3215 1404
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 629 763 834 1503 1570 2444 2876 3623 3891
Size: 134 71 669 67 874 432 747 268
Cuts at: 3891 629
Size: 1357
Fragments arranged by size:
1357 874 747 669 432 268 134 71
67
AflIII A'CryG_T
Cuts at: 1971 3075 4052 1971
Size: 1104 977 2538
Fragments arranged by size:
2538 1104 977
AgeI A'CCGG_T
Cuts at: 3007 3007
Size: 4619
AlwNI CAG_nnn'CTG
Cuts at: 738 741 857 1924 3491 738
Size: 3 116 1067 1567 1866
Fragments arranged by size:
1866 1567 1067 116 3
ApaI G_GGCC'C
Cuts at: 248 248
Size: 4619
ApaLI G'TGCA_C
Cuts at: 3389 3389
Size: 4619
ApoI r'AATT_y
Cuts at: 820 1510 1623 3972 4228 820
Size: 690 113 2349 256 1211
Fragments arranged by size:
2349 1211 690 256 113
AseI AT'TA_AT
Cuts at: 20 20
Size: 4619
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4619
AvaI C'yCGr_G
Cuts at: 1172 1639 1172
Size: 467 4152
BamHI G'GATC_C
Cuts at: 1644 1644
Size: 4619
BanI G'GyrC_C
Cuts at: 221 251 1283 1300 1635 2049 2297 2332 221
Size: 30 1032 17 335 414 248 35 2508
Fragments arranged by size:
2508 1032 414 335 248 35 30 17
BanII G_rGCy'C
Cuts at: 127 248 1077 1633 2663 127
Size: 121 829 556 1030 2083
Fragments arranged by size:
2083 1030 829 556 121
BbeI G_GCGC'C
Cuts at: 225 255 2053 2301 225
Size: 30 1798 248 2543
Fragments arranged by size:
2543 1798 248 30
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4585
BciVI GTATCCnnnnn_n'
Cuts at: 2517 3278 4619 2517
Size: 761 1341 2517
Fragments arranged by size:
2517 1341 761
BclI T'GATC_A
Cuts at: 2139 2139
Size: 4619
BglI GCCn_nnn'nGGC
Cuts at: 145 145
Size: 4619
BglII A'GATC_T
Cuts at: 2134 4171 2134
Size: 2037 2582
BlpI GC'TnA_GC
Cuts at: 1543 1731 1543
Size: 188 4431
Bme1580I G_kGCm'C
Cuts at: 181 248 2244 2337 3393 181
Size: 67 1996 93 1056 1407
Fragments arranged by size:
1996 1407 1056 93 67
BmrI ACTGGGnnnn_n'
Cuts at: 184 1573 1831 1912 2247 4391 184
Size: 1389 258 81 335 2144 412
Fragments arranged by size:
2144 1389 412 335 258 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2986 2986
Size: 4619
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 1408 325
Size: 1083 3536
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1795 2674 3166 3464 1795
Size: 879 492 298 2950
Fragments arranged by size:
2950 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4323 4323
Size: 4619
BsaAI yAC'GTr
Cuts at: 1972 1972
Size: 4619
BsaBI GATnn'nnATC
Cuts at: 6 914 2133 4170 6
Size: 908 1219 2037 455
Fragments arranged by size:
2037 1219 908 455
BsaHI Gr'CG_yC
Cuts at: 222 252 2050 2298 3000 4496 222
Size: 30 1798 248 702 1496 345
Fragments arranged by size:
1798 1496 702 345 248 30
BsaWI w'CCGG_w
Cuts at: 1374 1808 2329 3007 3281 3428 3839 1374
Size: 434 521 678 274 147 411 2154
Fragments arranged by size:
2154 678 521 434 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 604 637 604
Size: 33 4586
BseYI C'CCAG_C
Cuts at: 1279 2044 2802 3379 3875 1279
Size: 765 758 577 496 2023
Fragments arranged by size:
2023 765 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1234 1315 1234
Size: 81 4538
BsiEI CG_ry'CG
Cuts at: 104 2161 2207 3415 104
Size: 2057 46 1208 1308
Fragments arranged by size:
2057 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1165 1633 2411 2601 3393 1165
Size: 468 778 190 792 2391
Fragments arranged by size:
2391 792 778 468 190
BsmI GAATG_Cn'
Cuts at: 581 581
Size: 4619
Bsp1286I G_dGCh'C
Cuts at: 127 181 248 1077 1165 1633 2244 2337 2411
Size: 54 67 829 88 468 611 93 74
Cuts at: 2411 2601 2663 3393 127
Size: 190 62 730 1353
Fragments arranged by size:
1353 829 730 611 468 190 93 88
74 67 62 54
BspEI T'CCGG_A
Cuts at: 1374 3839 1374
Size: 2465 2154
BspHI T'CATG_A
Cuts at: 3795 3795
Size: 4619
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1298 1669 1883 2185 2566 1298
Size: 371 214 302 381 3351
Fragments arranged by size:
3351 381 371 302 214
BsrBI CCG'CTC
Cuts at: 50 2911 2965 4449 4614 50
Size: 2861 54 1484 165 55
Fragments arranged by size:
2861 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2531 2531
Size: 4619
BsrFI r'CCGG_y
Cuts at: 2617 2798 3007 2617
Size: 181 209 4229
Fragments arranged by size:
4229 209 181
BssSI C'ACGA_G
Cuts at: 2890 3248 2890
Size: 358 4261
BstAPI GCAn_nnn'nTGC
Cuts at: 1996 1996
Size: 4619
BstBI TT'CG_AA
Cuts at: 2980 2980
Size: 4619
BstXI CCAn_nnnn'nTGG
Cuts at: 430 430
Size: 4619
BstYI r'GATC_y
Cuts at: 1 1644 2111 2134 2469 2715 3727 3813 3825
Size: 1643 467 23 335 246 1012 86 12
Cuts at: 3825 4171 1
Size: 346 449
Fragments arranged by size:
1643 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1607 1607
Size: 4619
BtgI C'CryG_G
Cuts at: 808 808
Size: 4619
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 133 1083 2569 2750 2913 133
Size: 950 1486 181 163 1839
Fragments arranged by size:
1839 1486 950 181 163
BtsI GCAGTG_nn'
Cuts at: 155 1862 155
Size: 1707 2912
DraI TTT'AAA
Cuts at: 1618 1618
Size: 4619
DraIII CAC_nnn'GTG
Cuts at: 1349 1349
Size: 4619
DrdI GACnn_nn'nnGTC
Cuts at: 2325 3183 2325
Size: 858 3761
EaeI y'GGCC_r
Cuts at: 498 1143 1466 2204 2378 2769 2796 4141 498
Size: 645 323 738 174 391 27 1345 976
Fragments arranged by size:
1345 976 738 645 391 323 174 27
EagI C'GGCC_G
Cuts at: 2204 2204
Size: 4619
EarI CTCTTCn'nnn_
Cuts at: 559 853 1036 2642 2852 559
Size: 294 183 1606 210 2326
Fragments arranged by size:
2326 1606 294 210 183
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2827 3137 3283 4429 2827
Size: 310 146 1146 3017
Fragments arranged by size:
3017 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1631 1631
Size: 4619
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 629 763 834 1408 1503 1570 2444 2876
Size: 304 134 71 574 95 67 874 432
Cuts at: 2876 3623 3891 325
Size: 747 268 1053
Fragments arranged by size:
1053 874 747 574 432 304 268 134
95 71 67
EcoNI CCTnn'n_nnAGG
Cuts at: 1294 1294
Size: 4619
EcoO109I rG'GnC_Cy
Cuts at: 244 245 1338 1523 1758 244
Size: 1 1093 185 235 3105
Fragments arranged by size:
3105 1093 235 185 1
EcoRI G'AATT_C
Cuts at: 1623 1623
Size: 4619
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 424 585 2638 424
Size: 161 2053 2405
Fragments arranged by size:
2405 2053 161
FspI TGC'GCA
Cuts at: 2400 2400
Size: 4619
HaeII r_GCGC'y
Cuts at: 225 255 1330 2053 2301 3323 4302 225
Size: 30 1075 723 248 1022 979 542
Fragments arranged by size:
1075 1022 979 723 542 248 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 111 143 449 481 2461 2491 2493 2523 111
Size: 32 306 32 1980 30 2 30 2207
Fragments arranged by size:
2207 1980 306 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1658 1658
Size: 4619
KasI G'GCGC_C
Cuts at: 221 251 2049 2297 221
Size: 30 1798 248 2543
Fragments arranged by size:
2543 1798 248 30
KpnI G_GTAC'C
Cuts at: 1287 1304 1639 1287
Size: 17 335 4267
Fragments arranged by size:
4267 335 17
MluI A'CGCG_T
Cuts at: 4052 4052
Size: 4619
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3290 3474 3290
Size: 184 4435
MscI TGG'CCA
Cuts at: 500 1145 1468 2380 500
Size: 645 323 912 2739
Fragments arranged by size:
2739 912 645 323
MslI CAynn'nnrTG
Cuts at: 2608 2735 2608
Size: 127 4492
MspA1I CmG'CkG
Cuts at: 315 738 1730 2044 2404 3417 3662 315
Size: 423 992 314 360 1013 245 1272
Fragments arranged by size:
1272 1013 992 423 360 314 245
NaeI GCC'GGC
Cuts at: 2800 2800
Size: 4619
NarI GG'CG_CC
Cuts at: 222 252 2050 2298 222
Size: 30 1798 248 2543
Fragments arranged by size:
2543 1798 248 30
NcoI C'CATG_G
Cuts at: 808 808
Size: 4619
NdeI CA'TA_TG
Cuts at: 887 887
Size: 4619
NgoMIV G'CCGG_C
Cuts at: 2798 2798
Size: 4619
NspI r_CATG'y
Cuts at: 788 1451 1672 2172 3079 788
Size: 663 221 500 907 2328
Fragments arranged by size:
2328 907 663 500 221
PasI CC'CwG_GG
Cuts at: 1250 1250
Size: 4619
PciI A'CATG_T
Cuts at: 3075 3075
Size: 4619
PflMI CCAn_nnn'nTGG
Cuts at: 1349 1386 1349
Size: 37 4582
PmeI GTTT'AAAC
Cuts at: 1618 1618
Size: 4619
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 3758
Size: 32 4587
PpuMI rG'GwC_Cy
Cuts at: 1338 1338
Size: 4619
PspOMI G'GGCC_C
Cuts at: 244 244
Size: 4619
PstI C_TGCA'G
Cuts at: 372 924 1018 1666 2351 372
Size: 552 94 648 685 2640
Fragments arranged by size:
2640 685 648 552 94
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4619
PvuII CAG'CTG
Cuts at: 315 738 2044 2404 315
Size: 423 1306 360 2530
Fragments arranged by size:
2530 1306 423 360
RsrII CG'GwC_CG
Cuts at: 2814 2814
Size: 4619
SacI G_AGCT'C
Cuts at: 1633 1633
Size: 4619
SalI G'TCGA_C
Cuts at: 1656 1656
Size: 4619
SanDI GG'GwC_CC
Cuts at: 1338 1338
Size: 4619
SapI GCTCTTCn'nnn_
Cuts at: 559 2642 2852 559
Size: 2083 210 2326
Fragments arranged by size:
2326 2083 210
SbfI CC_TGCA'GG
Cuts at: 1666 1666
Size: 4619
ScaI AGT'ACT
Cuts at: 4136 4136
Size: 4619
SfcI C'TryA_G
Cuts at: 33 368 920 1014 1662 2347 3340 3531 33
Size: 335 552 94 648 685 993 191 1121
Fragments arranged by size:
1121 993 685 648 552 335 191 94
SfoI GGC'GCC
Cuts at: 223 253 2051 2299 223
Size: 30 1798 248 2543
Fragments arranged by size:
2543 1798 248 30
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4619
SmaI CCC'GGG
Cuts at: 1174 1641 1174
Size: 467 4152
SmlI C'TyrA_G
Cuts at: 1774 2689 3181 3443 1774
Size: 915 492 262 2950
Fragments arranged by size:
2950 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4619
SphI G_CATG'C
Cuts at: 1451 1672 2172 1451
Size: 221 500 3898
Fragments arranged by size:
3898 500 221
StyI C'CwwG_G
Cuts at: 264 808 1295 1753 2107 264
Size: 544 487 458 354 2776
Fragments arranged by size:
2776 544 487 458 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2202 2335 2794 3003 2202
Size: 133 459 209 3818
Fragments arranged by size:
3818 459 209 133
TatI w'GTAC_w
Cuts at: 1116 4134 1116
Size: 3018 1601
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 963 995 1356 1388 4136 4168 963
Size: 32 361 32 2748 32 1414
Fragments arranged by size:
2748 1414 361 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2416 2416
Size: 4619
XbaI T'CTAG_A
Cuts at: 1650 1650
Size: 4619
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2114 2114
Size: 4619
XmaI C'CCGG_G
Cuts at: 1172 1639 1172
Size: 467 4152
XmnI GAAnn'nnTTC
Cuts at: 581 581
Size: 4619
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PflMI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PfoI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI