Restriction map for
pF1KB5229
(Circular) (Six-base) MAPSORT of: pF1KB5229.seq Check: 8414 from: 1 to: 3992
pF1KB5229 3992 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 1030 1030
Size: 3992
Acc65I G'GTAC_C
Cuts at: 1008 1008
Size: 3992
AclI AA'CG_TT
Cuts at: 3832 3832
Size: 3992
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1004 1817 2249 2996 3264 1004
Size: 813 432 747 268 1732
Fragments arranged by size:
1732 813 747 432 268
AflIII A'CryG_T
Cuts at: 1344 2448 3425 1344
Size: 1104 977 1911
Fragments arranged by size:
1911 1104 977
AgeI A'CCGG_T
Cuts at: 2380 2380
Size: 3992
AlwNI CAG_nnn'CTG
Cuts at: 1297 2864 1297
Size: 1567 2425
ApaLI G'TGCA_C
Cuts at: 2762 2762
Size: 3992
ApoI r'AATT_y
Cuts at: 353 917 996 3345 3601 353
Size: 564 79 2349 256 744
Fragments arranged by size:
2349 744 564 256 79
AseI AT'TA_AT
Cuts at: 20 20
Size: 3992
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3992
AvaI C'yCGr_G
Cuts at: 1012 1012
Size: 3992
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 411 444 411
Size: 33 3959
BamHI G'GATC_C
Cuts at: 265 1017 265
Size: 752 3240
BanI G'GyrC_C
Cuts at: 1008 1422 1670 1705 1008
Size: 414 248 35 3295
Fragments arranged by size:
3295 414 248 35
BanII G_rGCy'C
Cuts at: 1006 2036 1006
Size: 1030 2962
BbeI G_GCGC'C
Cuts at: 1426 1674 1426
Size: 248 3744
BciVI GTATCCnnnnn_n'
Cuts at: 439 1890 2651 3992 439
Size: 1451 761 1341 439
Fragments arranged by size:
1451 1341 761 439
BclI T'GATC_A
Cuts at: 729 1512 729
Size: 783 3209
BglII A'GATC_T
Cuts at: 1507 3544 1507
Size: 2037 1955
BlpI GC'TnA_GC
Cuts at: 1104 1104
Size: 3992
Bme1580I G_kGCm'C
Cuts at: 1617 1710 2766 1617
Size: 93 1056 2843
Fragments arranged by size:
2843 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1204 1285 1620 3764 1204
Size: 81 335 2144 1432
Fragments arranged by size:
2144 1432 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2359 2359
Size: 3992
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1168 2047 2539 2837 1168
Size: 879 492 298 2323
Fragments arranged by size:
2323 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3696 3696
Size: 3992
BsaAI yAC'GTr
Cuts at: 1345 1345
Size: 3992
BsaBI GATnn'nnATC
Cuts at: 6 1506 3543 6
Size: 1500 2037 455
Fragments arranged by size:
2037 1500 455
BsaHI Gr'CG_yC
Cuts at: 1423 1671 2373 3869 1423
Size: 248 702 1496 1546
Fragments arranged by size:
1546 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1181 1702 2380 2654 2801 3212 1181
Size: 521 678 274 147 411 1961
Fragments arranged by size:
1961 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1417 2175 2752 3248 1417
Size: 758 577 496 2161
Fragments arranged by size:
2161 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 563 563
Size: 3992
BsiEI CG_ry'CG
Cuts at: 104 1534 1580 2788 104
Size: 1430 46 1208 1308
Fragments arranged by size:
1430 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1006 1784 1974 2766 1006
Size: 778 190 792 2232
Fragments arranged by size:
2232 792 778 190
BsmI GAATG_Cn'
Cuts at: 506 506
Size: 3992
Bsp1286I G_dGCh'C
Cuts at: 1006 1617 1710 1784 1974 2036 2766 1006
Size: 611 93 74 190 62 730 2232
Fragments arranged by size:
2232 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3212 3212
Size: 3992
BspHI T'CATG_A
Cuts at: 279 627 855 3168 279
Size: 348 228 2313 1103
Fragments arranged by size:
2313 1103 348 228
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1042 1256 1558 1939 1042
Size: 214 302 381 3095
Fragments arranged by size:
3095 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2284 2338 3822 3987 50
Size: 2234 54 1484 165 55
Fragments arranged by size:
2234 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1904 1904
Size: 3992
BsrFI r'CCGG_y
Cuts at: 1990 2171 2380 1990
Size: 181 209 3602
Fragments arranged by size:
3602 209 181
BssSI C'ACGA_G
Cuts at: 2263 2621 2263
Size: 358 3634
BstAPI GCAn_nnn'nTGC
Cuts at: 1369 1369
Size: 3992
BstBI TT'CG_AA
Cuts at: 2353 2353
Size: 3992
BstEII G'GTnAC_C
Cuts at: 756 756
Size: 3992
BstXI CCAn_nnnn'nTGG
Cuts at: 377 377
Size: 3992
BstYI r'GATC_y
Cuts at: 1 265 1017 1484 1507 1842 2088 3100 3186
Size: 264 752 467 23 335 246 1012 86
Cuts at: 3186 3198 3544 1
Size: 12 346 449
Fragments arranged by size:
1012 752 467 449 346 335 264 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3992
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1942 2123 2286 1942
Size: 181 163 3648
Fragments arranged by size:
3648 181 163
BtsI GCAGTG_nn'
Cuts at: 1235 1235
Size: 3992
DraI TTT'AAA
Cuts at: 207 821 991 207
Size: 614 170 3208
Fragments arranged by size:
3208 614 170
DrdI GACnn_nn'nnGTC
Cuts at: 1698 2556 1698
Size: 858 3134
EaeI y'GGCC_r
Cuts at: 1577 1751 2142 2169 3514 1577
Size: 174 391 27 1345 2055
Fragments arranged by size:
2055 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1577 1577
Size: 3992
EarI CTCTTCn'nnn_
Cuts at: 2015 2225 2015
Size: 210 3782
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2200 2510 2656 3802 2200
Size: 310 146 1146 2390
Fragments arranged by size:
2390 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1004 1004
Size: 3992
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1004 1817 2249 2996 3264 1004
Size: 813 432 747 268 1732
Fragments arranged by size:
1732 813 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1131 1131
Size: 3992
EcoRI G'AATT_C
Cuts at: 996 996
Size: 3992
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2011 2011
Size: 3992
FspI TGC'GCA
Cuts at: 1773 1773
Size: 3992
HaeII r_GCGC'y
Cuts at: 1426 1674 2696 3675 1426
Size: 248 1022 979 1743
Fragments arranged by size:
1743 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1834 1864 1866 1896 1834
Size: 30 2 30 3930
Fragments arranged by size:
3930 30 30 2
HincII GTy'rAC
Cuts at: 1031 1031
Size: 3992
KasI G'GCGC_C
Cuts at: 1422 1670 1422
Size: 248 3744
KpnI G_GTAC'C
Cuts at: 1012 1012
Size: 3992
MluI A'CGCG_T
Cuts at: 3425 3425
Size: 3992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2663 2847 2663
Size: 184 3808
MscI TGG'CCA
Cuts at: 1753 1753
Size: 3992
MslI CAynn'nnrTG
Cuts at: 903 1981 2108 903
Size: 1078 127 2787
Fragments arranged by size:
2787 1078 127
MspA1I CmG'CkG
Cuts at: 435 1103 1417 1777 2790 3035 435
Size: 668 314 360 1013 245 1392
Fragments arranged by size:
1392 1013 668 360 314 245
NaeI GCC'GGC
Cuts at: 2173 2173
Size: 3992
NarI GG'CG_CC
Cuts at: 1423 1671 1423
Size: 248 3744
NcoI C'CATG_G
Cuts at: 107 107
Size: 3992
NgoMIV G'CCGG_C
Cuts at: 2171 2171
Size: 3992
NspI r_CATG'y
Cuts at: 1045 1545 2452 1045
Size: 500 907 2585
Fragments arranged by size:
2585 907 500
PciI A'CATG_T
Cuts at: 2448 2448
Size: 3992
PmeI GTTT'AAAC
Cuts at: 991 991
Size: 3992
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 918 950 3131 3163 918
Size: 32 2181 32 1747
Fragments arranged by size:
2181 1747 32 32
PstI C_TGCA'G
Cuts at: 1039 1724 1039
Size: 685 3307
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3992
PvuII CAG'CTG
Cuts at: 435 1417 1777 435
Size: 982 360 2650
Fragments arranged by size:
2650 982 360
RsrII CG'GwC_CG
Cuts at: 2187 2187
Size: 3992
SacI G_AGCT'C
Cuts at: 1006 1006
Size: 3992
SalI G'TCGA_C
Cuts at: 1029 1029
Size: 3992
SapI GCTCTTCn'nnn_
Cuts at: 2015 2225 2015
Size: 210 3782
SbfI CC_TGCA'GG
Cuts at: 1039 1039
Size: 3992
ScaI AGT'ACT
Cuts at: 3509 3509
Size: 3992
SfcI C'TryA_G
Cuts at: 33 1035 1720 2713 2904 33
Size: 1002 685 993 191 1121
Fragments arranged by size:
1121 1002 993 685 191
SfoI GGC'GCC
Cuts at: 1424 1672 1424
Size: 248 3744
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3992
SmaI CCC'GGG
Cuts at: 1014 1014
Size: 3992
SmlI C'TyrA_G
Cuts at: 1147 2062 2554 2816 1147
Size: 915 492 262 2323
Fragments arranged by size:
2323 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3992
SphI G_CATG'C
Cuts at: 1045 1545 1045
Size: 500 3492
SspI AAT'ATT
Cuts at: 480 480
Size: 3992
StyI C'CwwG_G
Cuts at: 107 149 1126 1480 107
Size: 42 977 354 2619
Fragments arranged by size:
2619 977 354 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 744 1575 1708 2167 2376 744
Size: 831 133 459 209 2360
Fragments arranged by size:
2360 831 459 209 133
TatI w'GTAC_w
Cuts at: 3507 3507
Size: 3992
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3509 3541 3509
Size: 32 3960
Tth111I GACn'n_nGTC
Cuts at: 1789 1789
Size: 3992
XbaI T'CTAG_A
Cuts at: 1023 1023
Size: 3992
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1487 1487
Size: 3992
XmaI C'CCGG_G
Cuts at: 1012 1012
Size: 3992
XmnI GAAnn'nnTTC
Cuts at: 397 397
Size: 3992
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmBI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI