Restriction map for
pF1KB5228
(Circular) (Six-base) MAPSORT of: pF1KB5228.seq Check: 1383 from: 1 to: 4532
pF1KB5228 4532 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 162 1477 162
Size: 1315 3217
AccI GT'mk_AC
Cuts at: 1570 1570
Size: 4532
Acc65I G'GTAC_C
Cuts at: 1548 1548
Size: 4532
AclI AA'CG_TT
Cuts at: 4372 4372
Size: 4532
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1204 2357 2789 3142 3804 1204
Size: 1153 432 353 662 1932
Fragments arranged by size:
1932 1153 662 432 353
AfeI AGC'GCT
Cuts at: 827 827
Size: 4532
AflIII A'CryG_T
Cuts at: 1191 1884 3684 3965 1191
Size: 693 1800 281 1758
Fragments arranged by size:
1800 1758 693 281
AleI CACnn'nnGTG
Cuts at: 1297 1297
Size: 4532
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 201 313 782 201
Size: 112 469 3951
Fragments arranged by size:
3951 469 112
AlwNI CAG_nnn'CTG
Cuts at: 249 540 612 870 1391 1837 3275 249
Size: 291 72 258 521 446 1438 1506
Fragments arranged by size:
1506 1438 521 446 291 258 72
ApaI G_GGCC'C
Cuts at: 547 1345 1523 547
Size: 798 178 3556
Fragments arranged by size:
3556 798 178
ApaLI G'TGCA_C
Cuts at: 757 3370 757
Size: 2613 1919
ApoI r'AATT_y
Cuts at: 1536 3885 4141 1536
Size: 2349 256 1927
Fragments arranged by size:
2349 1927 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4532
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4532
AvaI C'yCGr_G
Cuts at: 546 688 1306 1338 1552 546
Size: 142 618 32 214 3526
Fragments arranged by size:
3526 618 214 142 32
BamHI G'GATC_C
Cuts at: 1557 1557
Size: 4532
BanI G'GyrC_C
Cuts at: 193 960 1450 1548 1962 2210 2245 193
Size: 767 490 98 414 248 35 2480
Fragments arranged by size:
2480 767 490 414 248 98 35
BanII G_rGCy'C
Cuts at: 218 547 1286 1315 1345 1523 1546 2576 218
Size: 329 739 29 30 178 23 1030 2174
Fragments arranged by size:
2174 1030 739 329 178 30 29 23
BbeI G_GCGC'C
Cuts at: 1966 2214 1966
Size: 248 4284
BbsI GAAGACnn'nnnn_
Cuts at: 1164 1164
Size: 4532
BbvCI CC'TCA_GC
Cuts at: 442 442
Size: 4532
BciVI GTATCCnnnnn_n'
Cuts at: 2430 3486 4532 2430
Size: 1056 1046 2430
Fragments arranged by size:
2430 1056 1046
BclI T'GATC_A
Cuts at: 2052 2052
Size: 4532
BglI GCCn_nnn'nGGC
Cuts at: 989 1098 1217 1400 1409 989
Size: 109 119 183 9 4112
Fragments arranged by size:
4112 183 119 109 9
BglII A'GATC_T
Cuts at: 2047 4084 2047
Size: 2037 2495
BlpI GC'TnA_GC
Cuts at: 1280 1644 1280
Size: 364 4168
Bme1580I G_kGCm'C
Cuts at: 263 547 689 761 861 912 965 1345 1455
Size: 284 142 72 100 51 53 380 110
Cuts at: 1455 1523 2157 2250 3374 263
Size: 68 634 93 1124 1421
Fragments arranged by size:
1421 1124 634 380 284 142 110 100
93 72 68 53 51
BmrI ACTGGGnnnn_n'
Cuts at: 1744 1825 2160 4304 1744
Size: 81 335 2144 1972
Fragments arranged by size:
2144 1972 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2899 2899
Size: 4532
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 448 663 1168 146
Size: 302 215 505 3510
Fragments arranged by size:
3510 505 302 215
Bpu10I CC'TnA_GC
Cuts at: 442 1446 442
Size: 1004 3528
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1708 2587 3301 3599 1708
Size: 879 714 298 2641
Fragments arranged by size:
2641 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4236 4236
Size: 4532
BsaAI yAC'GTr
Cuts at: 1885 1885
Size: 4532
BsaBI GATnn'nnATC
Cuts at: 6 2046 4083 6
Size: 2040 2037 455
Fragments arranged by size:
2040 2037 455
BsaHI Gr'CG_yC
Cuts at: 118 1963 2211 2913 4409 118
Size: 1845 248 702 1496 241
Fragments arranged by size:
1845 1496 702 248 241
BsaWI w'CCGG_w
Cuts at: 461 1721 2242 2920 3331 3478 3752 461
Size: 1260 521 678 411 147 274 1241
Fragments arranged by size:
1260 1241 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 661 691 661
Size: 30 4502
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 939 942 1102 939
Size: 3 160 4369
Fragments arranged by size:
4369 160 3
BseYI C'CCAG_C
Cuts at: 239 540 1095 1459 1957 2715 3380 3788 239
Size: 301 555 364 498 758 665 408 983
Fragments arranged by size:
983 758 665 555 498 408 364 301
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 355 496 646 694 718 283
Size: 72 141 150 48 24 4097
Fragments arranged by size:
4097 150 141 72 48 24
BsiEI CG_ry'CG
Cuts at: 104 723 2074 2120 3350 104
Size: 619 1351 46 1230 1286
Fragments arranged by size:
1351 1286 1230 619 46
BsiHKAI G_wGCw'C
Cuts at: 761 1546 2324 2514 3374 761
Size: 785 778 190 860 1919
Fragments arranged by size:
1919 860 785 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 730 730
Size: 4532
Bsp1286I G_dGCh'C
Cuts at: 218 263 547 689 761 861 912 965 1286
Size: 45 284 142 72 100 51 53 321
Cuts at: 1286 1315 1345 1455 1523 1546 2157 2250 2324
Size: 29 30 110 68 23 611 93 74
Cuts at: 2324 2514 2576 3374 218
Size: 190 62 798 1376
Fragments arranged by size:
1376 798 611 321 284 190 142 110
100 93 74 72 68 62 53 51
45 30 29 23
BspHI T'CATG_A
Cuts at: 791 2964 791
Size: 2173 2359
BspMI ACCTGCnnnn'nnnn_
Cuts at: 162 596 1031 1477 1582 1796 2098 2479 162
Size: 434 435 446 105 214 302 381 2215
Fragments arranged by size:
2215 446 435 434 381 302 214 105
BsrBI CCG'CTC
Cuts at: 50 1290 2824 2878 4362 4527 50
Size: 1240 1534 54 1484 165 55
Fragments arranged by size:
1534 1484 1240 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1182 2444 1182
Size: 1262 3270
BsrFI r'CCGG_y
Cuts at: 134 1045 1069 2530 2711 134
Size: 911 24 1461 181 1955
Fragments arranged by size:
1955 1461 911 181 24
BssHII G'CGCG_C
Cuts at: 693 693
Size: 4532
BssSI C'ACGA_G
Cuts at: 2803 3511 2803
Size: 708 3824
BstAPI GCAn_nnn'nTGC
Cuts at: 249 258 612 1177 1391 1909 249
Size: 9 354 565 214 518 2872
Fragments arranged by size:
2872 565 518 354 214 9
BstBI TT'CG_AA
Cuts at: 2893 2893
Size: 4532
BstYI r'GATC_y
Cuts at: 1 1557 2024 2047 2382 2628 2934 2946 3032
Size: 1556 467 23 335 246 306 12 86
Cuts at: 3032 4084 1
Size: 1052 449
Fragments arranged by size:
1556 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 871 871
Size: 4532
BtgI C'CryG_G
Cuts at: 263 486 558 702 942 1234 263
Size: 223 72 144 240 292 3561
Fragments arranged by size:
3561 292 240 223 144 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2482 2663 2826 2482
Size: 181 163 4188
Fragments arranged by size:
4188 181 163
BtsI GCAGTG_nn'
Cuts at: 610 1292 1304 1389 1775 610
Size: 682 12 85 386 3367
Fragments arranged by size:
3367 682 386 85 12
DraI TTT'AAA
Cuts at: 1531 1531
Size: 4532
DrdI GACnn_nn'nnGTC
Cuts at: 2238 3582 2238
Size: 1344 3188
EaeI y'GGCC_r
Cuts at: 783 990 1040 2117 2291 2682 2709 4054 783
Size: 207 50 1077 174 391 27 1345 1261
Fragments arranged by size:
1345 1261 1077 391 207 174 50 27
EagI C'GGCC_G
Cuts at: 2117 2117
Size: 4532
EarI CTCTTCn'nnn_
Cuts at: 106 661 2555 2765 106
Size: 555 1894 210 1873
Fragments arranged by size:
1894 1873 555 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2740 3482 3628 4342 2740
Size: 742 146 714 2930
Fragments arranged by size:
2930 742 714 146
EcoICRI GAG'CTC
Cuts at: 1544 1544
Size: 4532
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 448 663 1168 1204 2357 2789 3142 3804
Size: 302 215 505 36 1153 432 353 662
Cuts at: 3804 146
Size: 874
Fragments arranged by size:
1153 874 662 505 432 353 302 215
36
EcoO109I rG'GnC_Cy
Cuts at: 246 840 1342 1520 1671 246
Size: 594 502 178 151 3107
Fragments arranged by size:
3107 594 502 178 151
EcoRI G'AATT_C
Cuts at: 1536 1536
Size: 4532
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1181 2551 1181
Size: 1370 3162
FspI TGC'GCA
Cuts at: 2313 2313
Size: 4532
HaeII r_GCGC'y
Cuts at: 829 1498 1966 2214 3444 4215 829
Size: 669 468 248 1230 771 1146
Fragments arranged by size:
1230 1146 771 669 468 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 786 818 1123 1155 2374 2404 2406 2436 786
Size: 32 305 32 1219 30 2 30 2882
Fragments arranged by size:
2882 1219 305 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1571 1571
Size: 4532
KasI G'GCGC_C
Cuts at: 1962 2210 1962
Size: 248 4284
KpnI G_GTAC'C
Cuts at: 1552 1552
Size: 4532
MluI A'CGCG_T
Cuts at: 1191 3965 1191
Size: 2774 1758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3291 3475 3291
Size: 184 4348
MscI TGG'CCA
Cuts at: 1042 2293 1042
Size: 1251 3281
MslI CAynn'nnrTG
Cuts at: 377 764 1244 1297 2521 2648 377
Size: 387 480 53 1224 127 2261
Fragments arranged by size:
2261 1224 480 387 127 53
MspA1I CmG'CkG
Cuts at: 124 328 435 524 560 944 1095 1129 1415
Size: 204 107 89 36 384 151 34 286
Cuts at: 1415 1643 1957 2317 3101 3346 124
Size: 228 314 360 784 245 1310
Fragments arranged by size:
1310 784 384 360 314 286 245 228
204 151 107 89 36 34
NaeI GCC'GGC
Cuts at: 136 2713 136
Size: 2577 1955
NarI GG'CG_CC
Cuts at: 1963 2211 1963
Size: 248 4284
NgoMIV G'CCGG_C
Cuts at: 134 2711 134
Size: 2577 1955
NspI r_CATG'y
Cuts at: 322 769 1585 2085 3688 322
Size: 447 816 500 1603 1166
Fragments arranged by size:
1603 1166 816 500 447
PasI CC'CwG_GG
Cuts at: 890 1485 890
Size: 595 3937
PciI A'CATG_T
Cuts at: 3684 3684
Size: 4532
PflMI CCAn_nnn'nTGG
Cuts at: 365 492 365
Size: 127 4405
PfoI T'CCnGG_A
Cuts at: 425 425
Size: 4532
PmeI GTTT'AAAC
Cuts at: 1531 1531
Size: 4532
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2978 3010 2978
Size: 32 4500
PspOMI G'GGCC_C
Cuts at: 543 1341 1519 543
Size: 798 178 3556
Fragments arranged by size:
3556 798 178
PstI C_TGCA'G
Cuts at: 164 254 536 578 593 608 866 935 970
Size: 90 282 42 15 15 258 69 35
Cuts at: 970 1028 1299 1474 1579 2264 164
Size: 58 271 175 105 685 2432
Fragments arranged by size:
2432 685 282 271 258 175 105 90
69 58 42 35 15 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4532
PvuII CAG'CTG
Cuts at: 328 435 1957 2317 328
Size: 107 1522 360 2543
Fragments arranged by size:
2543 1522 360 107
RsrII CG'GwC_CG
Cuts at: 2727 2727
Size: 4532
SacI G_AGCT'C
Cuts at: 1546 1546
Size: 4532
SacII CC_GC'GG
Cuts at: 561 945 561
Size: 384 4148
SalI G'TCGA_C
Cuts at: 1569 1569
Size: 4532
SapI GCTCTTCn'nnn_
Cuts at: 2555 2765 2555
Size: 210 4322
SbfI CC_TGCA'GG
Cuts at: 254 536 593 1028 1579 254
Size: 282 57 435 551 3207
Fragments arranged by size:
3207 551 435 282 57
ScaI AGT'ACT
Cuts at: 1145 4049 1145
Size: 2904 1628
SfcI C'TryA_G
Cuts at: 33 160 250 532 574 589 604 862 931
Size: 127 90 282 42 15 15 258 69
Cuts at: 931 966 1024 1295 1470 1575 2260 3228 3419
Size: 35 58 271 175 105 685 968 191
Cuts at: 3419 33
Size: 1146
Fragments arranged by size:
1146 968 685 282 271 258 191 175
127 105 90 69 58 42 35 15
15
SfoI GGC'GCC
Cuts at: 1964 2212 1964
Size: 248 4284
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4532
SmaI CCC'GGG
Cuts at: 548 1554 548
Size: 1006 3526
SmlI C'TyrA_G
Cuts at: 1687 2602 3316 3578 1687
Size: 915 714 262 2641
Fragments arranged by size:
2641 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4532
SphI G_CATG'C
Cuts at: 769 1585 2085 769
Size: 816 500 3216
Fragments arranged by size:
3216 816 500
StuI AGG'CCT
Cuts at: 166 1138 166
Size: 972 3560
StyI C'CwwG_G
Cuts at: 218 780 1666 2020 218
Size: 562 886 354 2730
Fragments arranged by size:
2730 886 562 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 138 882 2115 2248 2707 2916 138
Size: 744 1233 133 459 209 1754
Fragments arranged by size:
1754 1233 744 459 209 133
TatI w'GTAC_w
Cuts at: 1143 4047 1143
Size: 2904 1628
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 641 673 1313 1345 4049 4081 641
Size: 32 640 32 2704 32 1092
Fragments arranged by size:
2704 1092 640 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2329 2329
Size: 4532
XbaI T'CTAG_A
Cuts at: 1563 1563
Size: 4532
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 840 923 2027 840
Size: 83 1104 3345
Fragments arranged by size:
3345 1104 83
XmaI C'CCGG_G
Cuts at: 546 1552 546
Size: 1006 3526
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AhdI AloI AscI AvrII BaeI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BspEI BsrGI
BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PmlI PpuMI PshAI PsiI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI