Restriction map for
pF1KB5220
(Circular) (Six-base) MAPSORT of: pF1KB5220.seq Check: 3748 from: 1 to: 5219
pF1KB5220 5219 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 2257 2257
Size: 5219
Acc65I G'GTAC_C
Cuts at: 2235 2235
Size: 5219
AclI AA'CG_TT
Cuts at: 5059 5059
Size: 5219
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 1861 3044 3476 4223 4491 1009
Size: 852 1183 432 747 268 1737
Fragments arranged by size:
1737 1183 852 747 432 268
AflIII A'CryG_T
Cuts at: 2066 2571 3675 4652 2066
Size: 505 1104 977 2633
Fragments arranged by size:
2633 1104 977 505
AgeI A'CCGG_T
Cuts at: 3607 3607
Size: 5219
AleI CACnn'nnGTG
Cuts at: 988 988
Size: 5219
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1006 1006
Size: 5219
AlwNI CAG_nnn'CTG
Cuts at: 1255 2023 2524 4091 1255
Size: 768 501 1567 2383
Fragments arranged by size:
2383 1567 768 501
ApaLI G'TGCA_C
Cuts at: 3989 3989
Size: 5219
ApoI r'AATT_y
Cuts at: 1044 2223 4572 4828 1044
Size: 1179 2349 256 1435
Fragments arranged by size:
2349 1435 1179 256
AseI AT'TA_AT
Cuts at: 20 1160 20
Size: 1140 4079
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5219
AvaI C'yCGr_G
Cuts at: 1907 2239 1907
Size: 332 4887
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2003 2036 2003
Size: 33 5186
BamHI G'GATC_C
Cuts at: 2244 2244
Size: 5219
BanI G'GyrC_C
Cuts at: 167 2235 2649 2897 2932 167
Size: 2068 414 248 35 2454
Fragments arranged by size:
2454 2068 414 248 35
BanII G_rGCy'C
Cuts at: 2233 3263 2233
Size: 1030 4189
BbeI G_GCGC'C
Cuts at: 2653 2901 2653
Size: 248 4971
BbsI GAAGACnn'nnnn_
Cuts at: 1012 1958 1012
Size: 946 4273
BbvCI CC'TCA_GC
Cuts at: 1586 1586
Size: 5219
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5185
BciVI GTATCCnnnnn_n'
Cuts at: 3117 3878 5219 3117
Size: 761 1341 3117
Fragments arranged by size:
3117 1341 761
BclI T'GATC_A
Cuts at: 2739 2739
Size: 5219
BfrBI ATG'CAT
Cuts at: 825 825
Size: 5219
BglII A'GATC_T
Cuts at: 855 1485 2734 4771 855
Size: 630 1249 2037 1303
Fragments arranged by size:
2037 1303 1249 630
BlpI GC'TnA_GC
Cuts at: 2331 2331
Size: 5219
Bme1580I G_kGCm'C
Cuts at: 2844 2937 3993 2844
Size: 93 1056 4070
Fragments arranged by size:
4070 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2011 2431 2512 2847 4991 2011
Size: 420 81 335 2144 2239
Fragments arranged by size:
2239 2144 420 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3586 3586
Size: 5219
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 908 1861 908
Size: 953 4266
Bpu10I CC'TnA_GC
Cuts at: 1586 1586
Size: 5219
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1690 2395 3274 3766 4064 1690
Size: 705 879 492 298 2845
Fragments arranged by size:
2845 879 705 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4923 4923
Size: 5219
BsaAI yAC'GTr
Cuts at: 2067 2572 2067
Size: 505 4714
BsaBI GATnn'nnATC
Cuts at: 6 2733 4770 6
Size: 2727 2037 455
Fragments arranged by size:
2727 2037 455
BsaHI Gr'CG_yC
Cuts at: 2650 2898 3600 5096 2650
Size: 248 702 1496 2773
Fragments arranged by size:
2773 1496 702 248
BsaWI w'CCGG_w
Cuts at: 412 2408 2929 3607 3881 4028 4439 412
Size: 1996 521 678 274 147 411 1192
Fragments arranged by size:
1996 1192 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1414 2101 1414
Size: 687 4532
BseYI C'CCAG_C
Cuts at: 2644 3402 3979 4475 2644
Size: 758 577 496 3388
Fragments arranged by size:
3388 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2761 2807 4015 104
Size: 2657 46 1208 1308
Fragments arranged by size:
2657 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2233 3011 3201 3993 2233
Size: 778 190 792 3459
Fragments arranged by size:
3459 792 778 190
BsmI GAATG_Cn'
Cuts at: 799 799
Size: 5219
Bsp1286I G_dGCh'C
Cuts at: 2233 2844 2937 3011 3201 3263 3993 2233
Size: 611 93 74 190 62 730 3459
Fragments arranged by size:
3459 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4439 4439
Size: 5219
BspHI T'CATG_A
Cuts at: 4395 4395
Size: 5219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2202 2269 2483 2785 3166 2202
Size: 67 214 302 381 4255
Fragments arranged by size:
4255 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 3511 3565 5049 5214 50
Size: 3461 54 1484 165 55
Fragments arranged by size:
3461 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3131 3131
Size: 5219
BsrFI r'CCGG_y
Cuts at: 170 3217 3398 3607 170
Size: 3047 181 209 1782
Fragments arranged by size:
3047 1782 209 181
BsrGI T'GTAC_A
Cuts at: 249 249
Size: 5219
BssSI C'ACGA_G
Cuts at: 3490 3848 3490
Size: 358 4861
BstAPI GCAn_nnn'nTGC
Cuts at: 2596 2596
Size: 5219
BstBI TT'CG_AA
Cuts at: 3580 3580
Size: 5219
BstYI r'GATC_y
Cuts at: 1 855 1167 1485 2040 2244 2711 2734 3069
Size: 854 312 318 555 204 467 23 335
Cuts at: 3069 3315 4327 4413 4425 4771 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 854 555 467 449 346 335 318
312 246 204 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1035 1035
Size: 5219
BtgI C'CryG_G
Cuts at: 434 434
Size: 5219
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3169 3350 3513 3169
Size: 181 163 4875
Fragments arranged by size:
4875 181 163
BtsI GCAGTG_nn'
Cuts at: 804 2462 804
Size: 1658 3561
DraI TTT'AAA
Cuts at: 2218 2218
Size: 5219
DrdI GACnn_nn'nnGTC
Cuts at: 2925 3783 2925
Size: 858 4361
EaeI y'GGCC_r
Cuts at: 1577 2804 2978 3369 3396 4741 1577
Size: 1227 174 391 27 1345 2055
Fragments arranged by size:
2055 1345 1227 391 174 27
EagI C'GGCC_G
Cuts at: 2804 2804
Size: 5219
EarI CTCTTCn'nnn_
Cuts at: 1283 3242 3452 1283
Size: 1959 210 3050
Fragments arranged by size:
3050 1959 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3427 3737 3883 5029 3427
Size: 310 146 1146 3617
Fragments arranged by size:
3617 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2231 2231
Size: 5219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 908 1009 1861 3044 3476 4223 4491 908
Size: 101 852 1183 432 747 268 1636
Fragments arranged by size:
1636 1183 852 747 432 268 101
EcoO109I rG'GnC_Cy
Cuts at: 2358 2358
Size: 5219
EcoRI G'AATT_C
Cuts at: 2223 2223
Size: 5219
EcoRV GAT'ATC
Cuts at: 1260 1260
Size: 5219
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1507 1618 3238 1507
Size: 111 1620 3488
Fragments arranged by size:
3488 1620 111
FspI TGC'GCA
Cuts at: 3000 3000
Size: 5219
HaeII r_GCGC'y
Cuts at: 906 2653 2901 3923 4902 906
Size: 1747 248 1022 979 1223
Fragments arranged by size:
1747 1223 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 632 664 1498 1530 1785 1817 2082 2114 3061
Size: 32 834 32 255 32 265 32 947
Cuts at: 3061 3091 3093 3123 632
Size: 30 2 30 2728
Fragments arranged by size:
2728 947 834 265 255 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1569 2258 1569
Size: 689 4530
HindIII A'AGCT_T
Cuts at: 1225 1225
Size: 5219
KasI G'GCGC_C
Cuts at: 2649 2897 2649
Size: 248 4971
KpnI G_GTAC'C
Cuts at: 2239 2239
Size: 5219
MluI A'CGCG_T
Cuts at: 4652 4652
Size: 5219
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1281 1830 3890 4074 1281
Size: 549 2060 184 2426
Fragments arranged by size:
2426 2060 549 184
MscI TGG'CCA
Cuts at: 1579 2980 1579
Size: 1401 3818
MslI CAynn'nnrTG
Cuts at: 988 1946 3208 3335 988
Size: 958 1262 127 2872
Fragments arranged by size:
2872 1262 958 127
MspA1I CmG'CkG
Cuts at: 2330 2644 3004 4017 4262 2330
Size: 314 360 1013 245 3287
Fragments arranged by size:
3287 1013 360 314 245
NaeI GCC'GGC
Cuts at: 172 3400 172
Size: 3228 1991
NarI GG'CG_CC
Cuts at: 2650 2898 2650
Size: 248 4971
NcoI C'CATG_G
Cuts at: 434 434
Size: 5219
NdeI CA'TA_TG
Cuts at: 402 402
Size: 5219
NgoMIV G'CCGG_C
Cuts at: 170 3398 170
Size: 3228 1991
NsiI A_TGCA'T
Cuts at: 827 827
Size: 5219
NspI r_CATG'y
Cuts at: 825 829 2272 2772 3679 825
Size: 4 1443 500 907 2365
Fragments arranged by size:
2365 1443 907 500 4
PciI A'CATG_T
Cuts at: 3675 3675
Size: 5219
PmeI GTTT'AAAC
Cuts at: 2218 2218
Size: 5219
PmlI CAC'GTG
Cuts at: 2067 2067
Size: 5219
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4358 4390 4358
Size: 32 5187
PsiI TTA'TAA
Cuts at: 338 338
Size: 5219
PstI C_TGCA'G
Cuts at: 2213 2266 2951 2213
Size: 53 685 4481
Fragments arranged by size:
4481 685 53
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5219
PvuII CAG'CTG
Cuts at: 2644 3004 2644
Size: 360 4859
RsrII CG'GwC_CG
Cuts at: 3414 3414
Size: 5219
SacI G_AGCT'C
Cuts at: 2233 2233
Size: 5219
SalI G'TCGA_C
Cuts at: 2256 2256
Size: 5219
SapI GCTCTTCn'nnn_
Cuts at: 3242 3452 3242
Size: 210 5009
SbfI CC_TGCA'GG
Cuts at: 2266 2266
Size: 5219
ScaI AGT'ACT
Cuts at: 4736 4736
Size: 5219
SfcI C'TryA_G
Cuts at: 33 788 2209 2262 2947 3940 4131 33
Size: 755 1421 53 685 993 191 1121
Fragments arranged by size:
1421 1121 993 755 685 191 53
SfoI GGC'GCC
Cuts at: 2651 2899 2651
Size: 248 4971
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5219
SmaI CCC'GGG
Cuts at: 2241 2241
Size: 5219
SmlI C'TyrA_G
Cuts at: 1669 2374 3289 3781 4043 1669
Size: 705 915 492 262 2845
Fragments arranged by size:
2845 915 705 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5219
SphI G_CATG'C
Cuts at: 2272 2772 2272
Size: 500 4719
SspI AAT'ATT
Cuts at: 1071 1140 1071
Size: 69 5150
StuI AGG'CCT
Cuts at: 1835 1835
Size: 5219
StyI C'CwwG_G
Cuts at: 434 945 965 2353 2707 434
Size: 511 20 1388 354 2946
Fragments arranged by size:
2946 1388 511 354 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2802 2935 3394 3603 2802
Size: 133 459 209 4418
Fragments arranged by size:
4418 459 209 133
TatI w'GTAC_w
Cuts at: 249 369 1108 4734 249
Size: 120 739 3626 734
Fragments arranged by size:
3626 739 734 120
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4736 4768 4736
Size: 32 5187
Tth111I GACn'n_nGTC
Cuts at: 3016 3016
Size: 5219
XbaI T'CTAG_A
Cuts at: 2250 2250
Size: 5219
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2714 2714
Size: 5219
XmaI C'CCGG_G
Cuts at: 2239 2239
Size: 5219
XmnI GAAnn'nnTTC
Cuts at: 443 575 2172 443
Size: 132 1597 3490
Fragments arranged by size:
3490 1597 132
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PciI PmeI PmlI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BglI BmgBI BmtI BsaXI BsgI BsiWI BsmBI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
FseI FspAI HpaI MfeI NheI NotI NruI PacI
PasI PflMI PfoI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI ZraI