Restriction map for
pF1KB5208
(Circular) (Six-base) MAPSORT of: pF1KB5208.seq Check: 9855 from: 1 to: 4220
pF1KB5208 4220 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 402 402
Size: 4220
AccI GT'mk_AC
Cuts at: 1258 1258
Size: 4220
Acc65I G'GTAC_C
Cuts at: 762 1236 762
Size: 474 3746
AclI AA'CG_TT
Cuts at: 935 4060 935
Size: 3125 1095
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2045 2477 2830 3492 2045
Size: 432 353 662 2773
Fragments arranged by size:
2773 662 432 353
AflIII A'CryG_T
Cuts at: 1572 3372 3653 1572
Size: 1800 281 2139
Fragments arranged by size:
2139 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1525 2963 1525
Size: 1438 2782
ApaLI G'TGCA_C
Cuts at: 3058 3058
Size: 4220
ApoI r'AATT_y
Cuts at: 1224 3573 3829 1224
Size: 2349 256 1615
Fragments arranged by size:
2349 1615 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4220
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4220
AvaI C'yCGr_G
Cuts at: 1240 1240
Size: 4220
BamHI G'GATC_C
Cuts at: 1245 1245
Size: 4220
BanI G'GyrC_C
Cuts at: 762 1236 1650 1898 1933 762
Size: 474 414 248 35 3049
Fragments arranged by size:
3049 474 414 248 35
BanII G_rGCy'C
Cuts at: 380 656 838 1234 2264 380
Size: 276 182 396 1030 2336
Fragments arranged by size:
2336 1030 396 276 182
BbeI G_GCGC'C
Cuts at: 1654 1902 1654
Size: 248 3972
BbsI GAAGACnn'nnnn_
Cuts at: 337 891 337
Size: 554 3666
BciVI GTATCCnnnnn_n'
Cuts at: 2118 3174 4220 2118
Size: 1056 1046 2118
Fragments arranged by size:
2118 1056 1046
BclI T'GATC_A
Cuts at: 590 1740 590
Size: 1150 3070
BglII A'GATC_T
Cuts at: 1735 3772 1735
Size: 2037 2183
BlpI GC'TnA_GC
Cuts at: 1332 1332
Size: 4220
Bme1580I G_kGCm'C
Cuts at: 1845 1938 3062 1845
Size: 93 1124 3003
Fragments arranged by size:
3003 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1432 1513 1848 3992 1432
Size: 81 335 2144 1660
Fragments arranged by size:
2144 1660 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2587 2587
Size: 4220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1396 2275 2989 3287 1396
Size: 879 714 298 2329
Fragments arranged by size:
2329 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 564 1156 1165 1190 3924 564
Size: 592 9 25 2734 860
Fragments arranged by size:
2734 860 592 25 9
BsaAI yAC'GTr
Cuts at: 1573 1573
Size: 4220
BsaBI GATnn'nnATC
Cuts at: 6 1734 3771 6
Size: 1728 2037 455
Fragments arranged by size:
2037 1728 455
BsaHI Gr'CG_yC
Cuts at: 1651 1899 2601 4097 1651
Size: 248 702 1496 1774
Fragments arranged by size:
1774 1496 702 248
BsaWI w'CCGG_w
Cuts at: 656 1409 1930 2608 3019 3166 3440 656
Size: 753 521 678 411 147 274 1436
Fragments arranged by size:
1436 753 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1119 1149 1119
Size: 30 4190
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 582 828 1177 582
Size: 246 349 3625
Fragments arranged by size:
3625 349 246
BseYI C'CCAG_C
Cuts at: 387 649 969 1645 2403 3068 3476 387
Size: 262 320 676 758 665 408 1131
Fragments arranged by size:
1131 758 676 665 408 320 262
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 669 669
Size: 4220
BsiEI CG_ry'CG
Cuts at: 104 525 1762 1808 3038 104
Size: 421 1237 46 1230 1286
Fragments arranged by size:
1286 1237 1230 421 46
BsiHKAI G_wGCw'C
Cuts at: 380 1234 2012 2202 3062 380
Size: 854 778 190 860 1538
Fragments arranged by size:
1538 860 854 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 951 951
Size: 4220
Bsp1286I G_dGCh'C
Cuts at: 380 656 838 1234 1845 1938 2012 2202 2264
Size: 276 182 396 611 93 74 190 62
Cuts at: 2264 3062 380
Size: 798 1538
Fragments arranged by size:
1538 798 611 396 276 190 182 93
74 62
BspEI T'CCGG_A
Cuts at: 656 656
Size: 4220
BspHI T'CATG_A
Cuts at: 461 512 638 2652 461
Size: 51 126 2014 2029
Fragments arranged by size:
2029 2014 126 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 402 1270 1484 1786 2167 402
Size: 868 214 302 381 2455
Fragments arranged by size:
2455 868 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1106 1125 2512 2566 4050 4215 50
Size: 1056 19 1387 54 1484 165 55
Fragments arranged by size:
1484 1387 1056 165 55 54 19
BsrDI GCAATG_nn'
Cuts at: 240 2132 240
Size: 1892 2328
BsrFI r'CCGG_y
Cuts at: 2218 2399 2218
Size: 181 4039
BssSI C'ACGA_G
Cuts at: 2491 3199 2491
Size: 708 3512
BstAPI GCAn_nnn'nTGC
Cuts at: 500 1597 500
Size: 1097 3123
BstBI TT'CG_AA
Cuts at: 2581 2581
Size: 4220
BstEII G'GTnAC_C
Cuts at: 867 867
Size: 4220
BstXI CCAn_nnnn'nTGG
Cuts at: 114 891 114
Size: 777 3443
BstYI r'GATC_y
Cuts at: 1 1245 1712 1735 2070 2316 2622 2634 2720
Size: 1244 467 23 335 246 306 12 86
Cuts at: 2720 3772 1
Size: 1052 449
Fragments arranged by size:
1244 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 596 950 1166 107
Size: 489 354 216 3161
Fragments arranged by size:
3161 489 354 216
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 351 2170 2351 2514 351
Size: 1819 181 163 2057
Fragments arranged by size:
2057 1819 181 163
BtsI GCAGTG_nn'
Cuts at: 1463 1463
Size: 4220
DraI TTT'AAA
Cuts at: 1219 1219
Size: 4220
DrdI GACnn_nn'nnGTC
Cuts at: 1926 3270 1926
Size: 1344 2876
EaeI y'GGCC_r
Cuts at: 362 557 581 697 953 1805 1979 2370 2397
Size: 195 24 116 256 852 174 391 27
Cuts at: 2397 3742 362
Size: 1345 840
Fragments arranged by size:
1345 852 840 391 256 195 174 116
27 24
EagI C'GGCC_G
Cuts at: 1805 1805
Size: 4220
EarI CTCTTCn'nnn_
Cuts at: 385 1063 2243 2453 385
Size: 678 1180 210 2152
Fragments arranged by size:
2152 1180 678 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 133 2428 3170 3316 4030 133
Size: 2295 742 146 714 323
Fragments arranged by size:
2295 742 714 323 146
EcoICRI GAG'CTC
Cuts at: 378 1232 378
Size: 854 3366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2045 2477 2830 3492 2045
Size: 432 353 662 2773
Fragments arranged by size:
2773 662 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 843 843
Size: 4220
EcoO109I rG'GnC_Cy
Cuts at: 1181 1359 1181
Size: 178 4042
EcoRI G'AATT_C
Cuts at: 1224 1224
Size: 4220
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1089 2239 1089
Size: 1150 3070
FspI TGC'GCA
Cuts at: 2001 2001
Size: 4220
HaeII r_GCGC'y
Cuts at: 1654 1902 3132 3903 1654
Size: 248 1230 771 1971
Fragments arranged by size:
1971 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2062 2092 2094 2124 2062
Size: 30 2 30 4158
Fragments arranged by size:
4158 30 30 2
HincII GTy'rAC
Cuts at: 188 1259 188
Size: 1071 3149
KasI G'GCGC_C
Cuts at: 1650 1898 1650
Size: 248 3972
KpnI G_GTAC'C
Cuts at: 766 1240 766
Size: 474 3746
MluI A'CGCG_T
Cuts at: 3653 3653
Size: 4220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2979 3163 2979
Size: 184 4036
MscI TGG'CCA
Cuts at: 364 699 955 1981 364
Size: 335 256 1026 2603
Fragments arranged by size:
2603 1026 335 256
MslI CAynn'nnrTG
Cuts at: 112 466 601 808 874 889 2209 2336 112
Size: 354 135 207 66 15 1320 127 1996
Fragments arranged by size:
1996 1320 354 207 135 127 66 15
MspA1I CmG'CkG
Cuts at: 683 1022 1331 1645 2005 2789 3034 683
Size: 339 309 314 360 784 245 1869
Fragments arranged by size:
1869 784 360 339 314 309 245
NaeI GCC'GGC
Cuts at: 2401 2401
Size: 4220
NarI GG'CG_CC
Cuts at: 1651 1899 1651
Size: 248 3972
NcoI C'CATG_G
Cuts at: 107 596 950 107
Size: 489 354 3377
Fragments arranged by size:
3377 489 354
NgoMIV G'CCGG_C
Cuts at: 2399 2399
Size: 4220
NspI r_CATG'y
Cuts at: 987 1273 1773 3376 987
Size: 286 500 1603 1831
Fragments arranged by size:
1831 1603 500 286
PciI A'CATG_T
Cuts at: 3372 3372
Size: 4220
PflMI CCAn_nnn'nTGG
Cuts at: 133 695 133
Size: 562 3658
PmeI GTTT'AAAC
Cuts at: 1219 1219
Size: 4220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2666 2698 2666
Size: 32 4188
PpuMI rG'GwC_Cy
Cuts at: 1181 1181
Size: 4220
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 712 744 712
Size: 32 4188
PstI C_TGCA'G
Cuts at: 1267 1952 1267
Size: 685 3535
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4220
PvuII CAG'CTG
Cuts at: 683 1022 1645 2005 683
Size: 339 623 360 2898
Fragments arranged by size:
2898 623 360 339
RsrII CG'GwC_CG
Cuts at: 2415 2415
Size: 4220
SacI G_AGCT'C
Cuts at: 380 1234 380
Size: 854 3366
SalI G'TCGA_C
Cuts at: 1257 1257
Size: 4220
SapI GCTCTTCn'nnn_
Cuts at: 385 1063 2243 2453 385
Size: 678 1180 210 2152
Fragments arranged by size:
2152 1180 678 210
SbfI CC_TGCA'GG
Cuts at: 1267 1267
Size: 4220
ScaI AGT'ACT
Cuts at: 3737 3737
Size: 4220
SfcI C'TryA_G
Cuts at: 33 1263 1948 2916 3107 33
Size: 1230 685 968 191 1146
Fragments arranged by size:
1230 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1652 1900 1652
Size: 248 3972
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4220
SmaI CCC'GGG
Cuts at: 1242 1242
Size: 4220
SmlI C'TyrA_G
Cuts at: 1375 2290 3004 3266 1375
Size: 915 714 262 2329
Fragments arranged by size:
2329 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4220
SphI G_CATG'C
Cuts at: 1273 1773 1273
Size: 500 3720
StyI C'CwwG_G
Cuts at: 107 596 950 1354 1708 107
Size: 489 354 404 354 2619
Fragments arranged by size:
2619 489 404 354 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 539 1803 1936 2395 2604 539
Size: 1264 133 459 209 2155
Fragments arranged by size:
2155 1264 459 209 133
TatI w'GTAC_w
Cuts at: 3735 3735
Size: 4220
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 815 847 3737 3769 815
Size: 32 2890 32 1266
Fragments arranged by size:
2890 1266 32 32
Tth111I GACn'n_nGTC
Cuts at: 2017 2017
Size: 4220
XbaI T'CTAG_A
Cuts at: 1251 1251
Size: 4220
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 433 453 1715 433
Size: 20 1262 2938
Fragments arranged by size:
2938 1262 20
XmaI C'CCGG_G
Cuts at: 1240 1240
Size: 4220
XmnI GAAnn'nnTTC
Cuts at: 1072 1072
Size: 4220
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsiWI BsmI BsrGI BssHII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI