Restriction map for
pF1KB5207
(Circular) (Six-base) MAPSORT of: pF1KB5207.seq Check: 8697 from: 1 to: 4700
pF1KB5207 4700 bp
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1585 1585
Size: 4700
AatII G_ACGT'C
Cuts at: 326 326
Size: 4700
AccI GT'mk_AC
Cuts at: 1738 1738
Size: 4700
Acc65I G'GTAC_C
Cuts at: 1551 1716 1551
Size: 165 4535
AclI AA'CG_TT
Cuts at: 4540 4540
Size: 4700
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 969 1579 1700 2525 2957 3310 3972 158
Size: 811 610 121 825 432 353 662 886
Fragments arranged by size:
886 825 811 662 610 432 353 121
AfeI AGC'GCT
Cuts at: 905 905
Size: 4700
AflIII A'CryG_T
Cuts at: 2052 3852 4133 2052
Size: 1800 281 2619
Fragments arranged by size:
2619 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 2005 3443 2005
Size: 1438 3262
ApaLI G'TGCA_C
Cuts at: 494 1475 3538 494
Size: 981 2063 1656
Fragments arranged by size:
2063 1656 981
ApoI r'AATT_y
Cuts at: 506 650 1704 4053 4309 506
Size: 144 1054 2349 256 897
Fragments arranged by size:
2349 1054 897 256 144
AseI AT'TA_AT
Cuts at: 20 20
Size: 4700
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4700
AvaI C'yCGr_G
Cuts at: 947 1720 947
Size: 773 3927
BamHI G'GATC_C
Cuts at: 1302 1332 1725 1302
Size: 30 393 4277
Fragments arranged by size:
4277 393 30
BanI G'GyrC_C
Cuts at: 277 1551 1716 2130 2378 2413 277
Size: 1274 165 414 248 35 2564
Fragments arranged by size:
2564 1274 414 248 165 35
BanII G_rGCy'C
Cuts at: 1714 2744 1714
Size: 1030 3670
BbeI G_GCGC'C
Cuts at: 281 2134 2382 281
Size: 1853 248 2599
Fragments arranged by size:
2599 1853 248
BbsI GAAGACnn'nnnn_
Cuts at: 1460 1518 1460
Size: 58 4642
BbvCI CC'TCA_GC
Cuts at: 611 1520 611
Size: 909 3791
BciVI GTATCCnnnnn_n'
Cuts at: 2598 3654 4700 2598
Size: 1056 1046 2598
Fragments arranged by size:
2598 1056 1046
BclI T'GATC_A
Cuts at: 752 2220 752
Size: 1468 3232
BglI GCCn_nnn'nGGC
Cuts at: 151 151
Size: 4700
BglII A'GATC_T
Cuts at: 2215 4252 2215
Size: 2037 2663
BlpI GC'TnA_GC
Cuts at: 194 1277 1812 194
Size: 1083 535 3082
Fragments arranged by size:
3082 1083 535
Bme1580I G_kGCm'C
Cuts at: 498 948 1479 2325 2418 3542 498
Size: 450 531 846 93 1124 1656
Fragments arranged by size:
1656 1124 846 531 450 93
BmrI ACTGGGnnnn_n'
Cuts at: 971 1912 1993 2328 4472 971
Size: 941 81 335 2144 1199
Fragments arranged by size:
2144 1199 941 335 81
BmtI G_CTAG'C
Cuts at: 688 688
Size: 4700
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3067 3067
Size: 4700
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 602 752 1393 602
Size: 150 641 3909
Fragments arranged by size:
3909 641 150
Bpu10I CC'TnA_GC
Cuts at: 611 1520 611
Size: 909 3791
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1876 2755 3469 3767 1876
Size: 879 714 298 2809
Fragments arranged by size:
2809 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 376 983 1558 4404 376
Size: 607 575 2846 672
Fragments arranged by size:
2846 672 607 575
BsaAI yAC'GTr
Cuts at: 2053 2053
Size: 4700
BsaBI GATnn'nnATC
Cuts at: 6 2214 4251 6
Size: 2208 2037 455
Fragments arranged by size:
2208 2037 455
BsaHI Gr'CG_yC
Cuts at: 278 323 2131 2379 3081 4577 278
Size: 45 1808 248 702 1496 401
Fragments arranged by size:
1808 1496 702 401 248 45
BsaWI w'CCGG_w
Cuts at: 1889 2410 3088 3499 3646 3920 1889
Size: 521 678 411 147 274 2669
Fragments arranged by size:
2669 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 189 407 1281 2125 2883 3548 3956 189
Size: 218 874 844 758 665 408 933
Fragments arranged by size:
933 874 844 758 665 408 218
BsiEI CG_ry'CG
Cuts at: 104 2242 2288 3518 104
Size: 2138 46 1230 1286
Fragments arranged by size:
2138 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 427 498 942 1479 1714 2492 2682 3542 427
Size: 71 444 537 235 778 190 860 1585
Fragments arranged by size:
1585 860 778 537 444 235 190 71
BsmI GAATG_Cn'
Cuts at: 1686 1686
Size: 4700
Bsp1286I G_dGCh'C
Cuts at: 427 498 942 948 1479 1714 2325 2418 2492
Size: 71 444 6 531 235 611 93 74
Cuts at: 2492 2682 2744 3542 427
Size: 190 62 798 1585
Fragments arranged by size:
1585 798 611 531 444 235 190 93
74 71 62 6
BspHI T'CATG_A
Cuts at: 539 3132 539
Size: 2593 2107
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1585 1750 1964 2266 2647 1585
Size: 165 214 302 381 3638
Fragments arranged by size:
3638 381 302 214 165
BsrBI CCG'CTC
Cuts at: 50 214 2992 3046 4530 4695 50
Size: 164 2778 54 1484 165 55
Fragments arranged by size:
2778 1484 165 164 55 54
BsrDI GCAATG_nn'
Cuts at: 843 1271 1418 2612 843
Size: 428 147 1194 2931
Fragments arranged by size:
2931 1194 428 147
BsrFI r'CCGG_y
Cuts at: 2698 2879 2698
Size: 181 4519
BsrGI T'GTAC_A
Cuts at: 1176 1176
Size: 4700
BssSI C'ACGA_G
Cuts at: 941 2971 3679 941
Size: 2030 708 1962
Fragments arranged by size:
2030 1962 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2077 2077
Size: 4700
BstBI TT'CG_AA
Cuts at: 3061 3061
Size: 4700
BstXI CCAn_nnnn'nTGG
Cuts at: 1122 1122
Size: 4700
BstYI r'GATC_y
Cuts at: 1 1162 1302 1332 1385 1725 2192 2215 2550
Size: 1161 140 30 53 340 467 23 335
Cuts at: 2550 2796 3102 3114 3200 4252 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1161 1052 467 449 340 335 306 246
140 86 53 30 23 12
BtgI C'CryG_G
Cuts at: 786 1371 786
Size: 585 4115
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2650 2831 2994 2650
Size: 181 163 4356
Fragments arranged by size:
4356 181 163
BtsI GCAGTG_nn'
Cuts at: 320 1356 1943 320
Size: 1036 587 3077
Fragments arranged by size:
3077 1036 587
DraI TTT'AAA
Cuts at: 1699 1699
Size: 4700
DraIII CAC_nnn'GTG
Cuts at: 937 937
Size: 4700
DrdI GACnn_nn'nnGTC
Cuts at: 2406 3750 2406
Size: 1344 3356
EaeI y'GGCC_r
Cuts at: 131 1112 2285 2459 2850 2877 4222 131
Size: 981 1173 174 391 27 1345 609
Fragments arranged by size:
1345 1173 981 609 391 174 27
EagI C'GGCC_G
Cuts at: 2285 2285
Size: 4700
EarI CTCTTCn'nnn_
Cuts at: 1676 2723 2933 1676
Size: 1047 210 3443
Fragments arranged by size:
3443 1047 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2908 3650 3796 4510 2908
Size: 742 146 714 3098
Fragments arranged by size:
3098 742 714 146
EcoICRI GAG'CTC
Cuts at: 1712 1712
Size: 4700
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 602 752 969 1393 1579 1700 2525 2957
Size: 444 150 217 424 186 121 825 432
Cuts at: 2957 3310 3972 158
Size: 353 662 886
Fragments arranged by size:
886 825 662 444 432 424 353 217
186 150 121
EcoO109I rG'GnC_Cy
Cuts at: 152 574 824 1639 1839 152
Size: 422 250 815 200 3013
Fragments arranged by size:
3013 815 422 250 200
EcoRI G'AATT_C
Cuts at: 1704 1704
Size: 4700
EcoRV GAT'ATC
Cuts at: 1416 1416
Size: 4700
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2719 2719
Size: 4700
FspI TGC'GCA
Cuts at: 2481 2481
Size: 4700
HaeII r_GCGC'y
Cuts at: 281 907 2134 2382 3612 4383 281
Size: 626 1227 248 1230 771 598
Fragments arranged by size:
1230 1227 771 626 598 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 563 595 877 909 1303 1335 1387 1419 1675
Size: 32 282 32 394 32 52 32 256
Cuts at: 1675 1707 2542 2572 2574 2604 563
Size: 32 835 30 2 30 2659
Fragments arranged by size:
2659 835 394 282 256 52 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1463 1739 1463
Size: 276 4424
KasI G'GCGC_C
Cuts at: 277 2130 2378 277
Size: 1853 248 2599
Fragments arranged by size:
2599 1853 248
KpnI G_GTAC'C
Cuts at: 1555 1720 1555
Size: 165 4535
MluI A'CGCG_T
Cuts at: 4133 4133
Size: 4700
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 241 3459 3643 241
Size: 3218 184 1298
Fragments arranged by size:
3218 1298 184
MscI TGG'CCA
Cuts at: 1114 2461 1114
Size: 1347 3353
MslI CAynn'nnrTG
Cuts at: 416 631 1120 1244 1361 2689 2816 416
Size: 215 489 124 117 1328 127 2300
Fragments arranged by size:
2300 1328 489 215 127 124 117
MspA1I CmG'CkG
Cuts at: 136 1297 1811 2125 2485 3269 3514 136
Size: 1161 514 314 360 784 245 1322
Fragments arranged by size:
1322 1161 784 514 360 314 245
NaeI GCC'GGC
Cuts at: 2881 2881
Size: 4700
NarI GG'CG_CC
Cuts at: 278 2131 2379 278
Size: 1853 248 2599
Fragments arranged by size:
2599 1853 248
NcoI C'CATG_G
Cuts at: 786 786
Size: 4700
NdeI CA'TA_TG
Cuts at: 1538 1538
Size: 4700
NgoMIV G'CCGG_C
Cuts at: 2879 2879
Size: 4700
NheI G'CTAG_C
Cuts at: 684 684
Size: 4700
NspI r_CATG'y
Cuts at: 1753 2253 3856 1753
Size: 500 1603 2597
Fragments arranged by size:
2597 1603 500
PciI A'CATG_T
Cuts at: 3852 3852
Size: 4700
PflMI CCAn_nnn'nTGG
Cuts at: 414 414
Size: 4700
PfoI T'CCnGG_A
Cuts at: 1409 1409
Size: 4700
PmeI GTTT'AAAC
Cuts at: 1699 1699
Size: 4700
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3146 3178 3146
Size: 32 4668
PpuMI rG'GwC_Cy
Cuts at: 574 824 1639 574
Size: 250 815 3635
Fragments arranged by size:
3635 815 250
PshAI GACnn'nnGTC
Cuts at: 223 223
Size: 4700
PstI C_TGCA'G
Cuts at: 1394 1747 2432 1394
Size: 353 685 3662
Fragments arranged by size:
3662 685 353
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4700
PvuII CAG'CTG
Cuts at: 1297 2125 2485 1297
Size: 828 360 3512
Fragments arranged by size:
3512 828 360
RsrII CG'GwC_CG
Cuts at: 2895 2895
Size: 4700
SacI G_AGCT'C
Cuts at: 1714 1714
Size: 4700
SalI G'TCGA_C
Cuts at: 1737 1737
Size: 4700
SanDI GG'GwC_CC
Cuts at: 1639 1639
Size: 4700
SapI GCTCTTCn'nnn_
Cuts at: 1676 2723 2933 1676
Size: 1047 210 3443
Fragments arranged by size:
3443 1047 210
SbfI CC_TGCA'GG
Cuts at: 1394 1747 1394
Size: 353 4347
ScaI AGT'ACT
Cuts at: 482 4217 482
Size: 3735 965
SexAI A'CCwGG_T
Cuts at: 933 933
Size: 4700
SfcI C'TryA_G
Cuts at: 33 1390 1743 2428 3396 3587 33
Size: 1357 353 685 968 191 1146
Fragments arranged by size:
1357 1146 968 685 353 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 151 151
Size: 4700
SfoI GGC'GCC
Cuts at: 279 2132 2380 279
Size: 1853 248 2599
Fragments arranged by size:
2599 1853 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4700
SmaI CCC'GGG
Cuts at: 949 1722 949
Size: 773 3927
SmlI C'TyrA_G
Cuts at: 1855 2770 3484 3746 1855
Size: 915 714 262 2809
Fragments arranged by size:
2809 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4700
SphI G_CATG'C
Cuts at: 1753 2253 1753
Size: 500 4200
StuI AGG'CCT
Cuts at: 1624 1624
Size: 4700
StyI C'CwwG_G
Cuts at: 698 786 1834 2188 698
Size: 88 1048 354 3210
Fragments arranged by size:
3210 1048 354 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 406 1057 2283 2416 2875 3084 406
Size: 651 1226 133 459 209 2022
Fragments arranged by size:
2022 1226 651 459 209 133
TatI w'GTAC_w
Cuts at: 480 747 1008 1176 1257 4215 480
Size: 267 261 168 81 2958 965
Fragments arranged by size:
2958 965 267 261 168 81
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4217 4249 4217
Size: 32 4668
Tth111I GACn'n_nGTC
Cuts at: 1467 2497 1467
Size: 1030 3670
XbaI T'CTAG_A
Cuts at: 1308 1731 1308
Size: 423 4277
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 393 1278 1431 2195 393
Size: 885 153 764 2898
Fragments arranged by size:
2898 885 764 153
XmaI C'CCGG_G
Cuts at: 947 1720 947
Size: 773 3927
XmnI GAAnn'nnTTC
Cuts at: 1083 1083
Size: 4700
ZraI GAC'GTC
Cuts at: 324 324
Size: 4700
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmgBI BsaXI BseRI BsgI
BsiWI BsmBI BspEI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI EcoNI FseI FspAI HindIII HpaI MfeI NotI
NruI NsiI PacI PasI PmlI PsiI PspOMI PspXI
PsrI SacII SgrAI SnaBI SrfI SspI SwaI XhoI