Restriction map for
pF1KB5199
(Circular) (Six-base) MAPSORT of: pF1KB5199.seq Check: 9274 from: 1 to: 4469
pF1KB5199 4469 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 842 842
Size: 4469
AatII G_ACGT'C
Cuts at: 1043 1043
Size: 4469
AccI GT'mk_AC
Cuts at: 686 1043 1507 686
Size: 357 464 3648
Fragments arranged by size:
3648 464 357
Acc65I G'GTAC_C
Cuts at: 1485 1485
Size: 4469
AclI AA'CG_TT
Cuts at: 4309 4309
Size: 4469
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2294 2726 3079 3741 2294
Size: 432 353 662 3022
Fragments arranged by size:
3022 662 432 353
AflIII A'CryG_T
Cuts at: 1821 3621 3902 1821
Size: 1800 281 2388
Fragments arranged by size:
2388 1800 281
AleI CACnn'nnGTG
Cuts at: 964 964
Size: 4469
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 778 810 778
Size: 32 4437
AlwNI CAG_nnn'CTG
Cuts at: 1227 1774 3212 1227
Size: 547 1438 2484
Fragments arranged by size:
2484 1438 547
ApaLI G'TGCA_C
Cuts at: 3307 3307
Size: 4469
ApoI r'AATT_y
Cuts at: 1473 3822 4078 1473
Size: 2349 256 1864
Fragments arranged by size:
2349 1864 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4469
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4469
AvaI C'yCGr_G
Cuts at: 1257 1489 1257
Size: 232 4237
BamHI G'GATC_C
Cuts at: 1494 1494
Size: 4469
BanI G'GyrC_C
Cuts at: 169 933 1485 1899 2147 2182 169
Size: 764 552 414 248 35 2456
Fragments arranged by size:
2456 764 552 414 248 35
BanII G_rGCy'C
Cuts at: 208 1276 1483 2513 208
Size: 1068 207 1030 2164
Fragments arranged by size:
2164 1068 1030 207
BbeI G_GCGC'C
Cuts at: 1903 2151 1903
Size: 248 4221
BbsI GAAGACnn'nnnn_
Cuts at: 1056 1056
Size: 4469
BciVI GTATCCnnnnn_n'
Cuts at: 2367 3423 4469 2367
Size: 1056 1046 2367
Fragments arranged by size:
2367 1056 1046
BclI T'GATC_A
Cuts at: 1989 1989
Size: 4469
BglI GCCn_nnn'nGGC
Cuts at: 123 499 1030 1346 123
Size: 376 531 316 3246
Fragments arranged by size:
3246 531 376 316
BglII A'GATC_T
Cuts at: 218 1984 4021 218
Size: 1766 2037 666
Fragments arranged by size:
2037 1766 666
BlpI GC'TnA_GC
Cuts at: 817 1581 817
Size: 764 3705
Bme1580I G_kGCm'C
Cuts at: 938 999 1359 2094 2187 3311 938
Size: 61 360 735 93 1124 2096
Fragments arranged by size:
2096 1124 735 360 93 61
BmgBI CAC'GTC
Cuts at: 516 1173 516
Size: 657 3812
BmrI ACTGGGnnnn_n'
Cuts at: 1455 1681 1762 2097 4241 1455
Size: 226 81 335 2144 1683
Fragments arranged by size:
2144 1683 335 226 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2836 2836
Size: 4469
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 337 673 1134 235
Size: 102 336 461 3570
Fragments arranged by size:
3570 461 336 102
Bpu10I CC'TnA_GC
Cuts at: 1332 1363 1332
Size: 31 4438
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1645 2524 3238 3536 1645
Size: 879 714 298 2578
Fragments arranged by size:
2578 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 421 912 1152 4173 421
Size: 491 240 3021 717
Fragments arranged by size:
3021 717 491 240
BsaAI yAC'GTr
Cuts at: 201 1822 201
Size: 1621 2848
BsaBI GATnn'nnATC
Cuts at: 6 1983 4020 6
Size: 1977 2037 455
Fragments arranged by size:
2037 1977 455
BsaHI Gr'CG_yC
Cuts at: 1040 1214 1900 2148 2850 4346 1040
Size: 174 686 248 702 1496 1163
Fragments arranged by size:
1496 1163 702 686 248 174
BsaWI w'CCGG_w
Cuts at: 1658 2179 2857 3268 3415 3689 1658
Size: 521 678 411 147 274 2438
Fragments arranged by size:
2438 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 190 220 862 892 190
Size: 30 642 30 3767
Fragments arranged by size:
3767 642 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 136 993 136
Size: 857 3612
BseYI C'CCAG_C
Cuts at: 1894 2652 3317 3725 1894
Size: 758 665 408 2638
Fragments arranged by size:
2638 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 808 364
Size: 444 4025
BsiEI CG_ry'CG
Cuts at: 104 1430 2011 2057 3287 104
Size: 1326 581 46 1230 1286
Fragments arranged by size:
1326 1286 1230 581 46
BsiHKAI G_wGCw'C
Cuts at: 713 1483 2261 2451 3311 713
Size: 770 778 190 860 1871
Fragments arranged by size:
1871 860 778 770 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1300 1300
Size: 4469
Bsp1286I G_dGCh'C
Cuts at: 208 713 938 999 1276 1359 1483 2094 2187
Size: 505 225 61 277 83 124 611 93
Cuts at: 2187 2261 2451 2513 3311 208
Size: 74 190 62 798 1366
Fragments arranged by size:
1366 798 611 505 277 225 190 124
93 83 74 62 61
BspHI T'CATG_A
Cuts at: 2901 2901
Size: 4469
BspMI ACCTGCnnnn'nnnn_
Cuts at: 842 1519 1733 2035 2416 842
Size: 677 214 302 381 2895
Fragments arranged by size:
2895 677 381 302 214
BsrBI CCG'CTC
Cuts at: 50 138 1001 1331 2761 2815 4299 4464 50
Size: 88 863 330 1430 54 1484 165 55
Fragments arranged by size:
1484 1430 863 330 165 88 55 54
BsrDI GCAATG_nn'
Cuts at: 2381 2381
Size: 4469
BsrFI r'CCGG_y
Cuts at: 253 1210 1241 2467 2648 253
Size: 957 31 1226 181 2074
Fragments arranged by size:
2074 1226 957 181 31
BsrGI T'GTAC_A
Cuts at: 566 854 566
Size: 288 4181
BssHII G'CGCG_C
Cuts at: 152 152
Size: 4469
BssSI C'ACGA_G
Cuts at: 2740 3448 2740
Size: 708 3761
BstAPI GCAn_nnn'nTGC
Cuts at: 1846 1846
Size: 4469
BstBI TT'CG_AA
Cuts at: 2830 2830
Size: 4469
BstXI CCAn_nnnn'nTGG
Cuts at: 987 987
Size: 4469
BstYI r'GATC_y
Cuts at: 1 218 1052 1494 1961 1984 2319 2565 2871
Size: 217 834 442 467 23 335 246 306
Cuts at: 2871 2883 2969 4021 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 834 467 449 442 335 306 246
217 86 23 12
BtgI C'CryG_G
Cuts at: 107 165 623 717 773 811 846 962 1012
Size: 58 458 94 56 38 35 116 50
Cuts at: 1012 1460 107
Size: 448 3116
Fragments arranged by size:
3116 458 448 116 94 58 56 50
38 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 193 2419 2600 2763 193
Size: 2226 181 163 1899
Fragments arranged by size:
2226 1899 181 163
BtsI GCAGTG_nn'
Cuts at: 1712 1712
Size: 4469
DraI TTT'AAA
Cuts at: 1468 1468
Size: 4469
DraIII CAC_nnn'GTG
Cuts at: 516 516
Size: 4469
DrdI GACnn_nn'nnGTC
Cuts at: 2175 3519 2175
Size: 1344 3125
EaeI y'GGCC_r
Cuts at: 323 611 801 1229 1427 2054 2228 2619 2646
Size: 288 190 428 198 627 174 391 27
Cuts at: 2646 3991 323
Size: 1345 801
Fragments arranged by size:
1345 801 627 428 391 288 198 190
174 27
EagI C'GGCC_G
Cuts at: 1427 2054 1427
Size: 627 3842
EarI CTCTTCn'nnn_
Cuts at: 1198 2492 2702 1198
Size: 1294 210 2965
Fragments arranged by size:
2965 1294 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2677 3419 3565 4279 2677
Size: 742 146 714 2867
Fragments arranged by size:
2867 742 714 146
EcoICRI GAG'CTC
Cuts at: 1481 1481
Size: 4469
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 337 673 1134 2294 2726 3079 3741 235
Size: 102 336 461 1160 432 353 662 963
Fragments arranged by size:
1160 963 662 461 432 353 336 102
EcoO109I rG'GnC_Cy
Cuts at: 289 1608 289
Size: 1319 3150
EcoRI G'AATT_C
Cuts at: 1473 1473
Size: 4469
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2488 2488
Size: 4469
FspI TGC'GCA
Cuts at: 2250 2250
Size: 4469
HaeII r_GCGC'y
Cuts at: 1251 1903 2151 3381 4152 1251
Size: 652 248 1230 771 1568
Fragments arranged by size:
1568 1230 771 652 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 325 357 2311 2341 2343 2373 325
Size: 32 1954 30 2 30 2421
Fragments arranged by size:
2421 1954 32 30 30 2
HincII GTy'rAC
Cuts at: 1508 1508
Size: 4469
KasI G'GCGC_C
Cuts at: 1899 2147 1899
Size: 248 4221
KpnI G_GTAC'C
Cuts at: 1489 1489
Size: 4469
MluI A'CGCG_T
Cuts at: 3902 3902
Size: 4469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 233 677 3228 3412 233
Size: 444 2551 184 1290
Fragments arranged by size:
2551 1290 444 184
MscI TGG'CCA
Cuts at: 325 613 803 1231 2230 325
Size: 288 190 428 999 2564
Fragments arranged by size:
2564 999 428 288 190
MslI CAynn'nnrTG
Cuts at: 370 964 985 2458 2585 370
Size: 594 21 1473 127 2254
Fragments arranged by size:
2254 1473 594 127 21
MspA1I CmG'CkG
Cuts at: 167 738 799 848 1580 1894 2254 3038 3283
Size: 571 61 49 732 314 360 784 245
Cuts at: 3283 167
Size: 1353
Fragments arranged by size:
1353 784 732 571 360 314 245 61
49
NaeI GCC'GGC
Cuts at: 255 1212 1243 2650 255
Size: 957 31 1407 2074
Fragments arranged by size:
2074 1407 957 31
NarI GG'CG_CC
Cuts at: 1900 2148 1900
Size: 248 4221
NcoI C'CATG_G
Cuts at: 107 623 1460 107
Size: 516 837 3116
Fragments arranged by size:
3116 837 516
NgoMIV G'CCGG_C
Cuts at: 253 1210 1241 2648 253
Size: 957 31 1407 2074
Fragments arranged by size:
2074 1407 957 31
NspI r_CATG'y
Cuts at: 645 876 1522 2022 3625 645
Size: 231 646 500 1603 1489
Fragments arranged by size:
1603 1489 646 500 231
PasI CC'CwG_GG
Cuts at: 432 432
Size: 4469
PciI A'CATG_T
Cuts at: 3621 3621
Size: 4469
PflMI CCAn_nnn'nTGG
Cuts at: 723 902 723
Size: 179 4290
PmeI GTTT'AAAC
Cuts at: 1468 1468
Size: 4469
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2915 2947 2915
Size: 32 4437
PstI C_TGCA'G
Cuts at: 548 737 1516 2201 548
Size: 189 779 685 2816
Fragments arranged by size:
2816 779 685 189
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4469
PvuII CAG'CTG
Cuts at: 738 1894 2254 738
Size: 1156 360 2953
Fragments arranged by size:
2953 1156 360
RsrII CG'GwC_CG
Cuts at: 2664 2664
Size: 4469
SacI G_AGCT'C
Cuts at: 1483 1483
Size: 4469
SacII CC_GC'GG
Cuts at: 168 849 168
Size: 681 3788
SalI G'TCGA_C
Cuts at: 1506 1506
Size: 4469
SapI GCTCTTCn'nnn_
Cuts at: 2492 2702 2492
Size: 210 4259
SbfI CC_TGCA'GG
Cuts at: 1516 1516
Size: 4469
ScaI AGT'ACT
Cuts at: 3986 3986
Size: 4469
SfcI C'TryA_G
Cuts at: 33 544 733 1116 1278 1512 2197 3165 3356
Size: 511 189 383 162 234 685 968 191
Cuts at: 3356 33
Size: 1146
Fragments arranged by size:
1146 968 685 511 383 234 191 189
162
SfoI GGC'GCC
Cuts at: 1901 2149 1901
Size: 248 4221
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4469
SmaI CCC'GGG
Cuts at: 1259 1491 1259
Size: 232 4237
SmlI C'TyrA_G
Cuts at: 1624 2539 3253 3515 1624
Size: 915 714 262 2578
Fragments arranged by size:
2578 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4469
SphI G_CATG'C
Cuts at: 645 1522 2022 645
Size: 877 500 3092
Fragments arranged by size:
3092 877 500
StyI C'CwwG_G
Cuts at: 107 623 1136 1460 1603 1957 107
Size: 516 513 324 143 354 2619
Fragments arranged by size:
2619 516 513 354 324 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2052 2185 2644 2853 2052
Size: 133 459 209 3668
Fragments arranged by size:
3668 459 209 133
TatI w'GTAC_w
Cuts at: 359 566 854 3984 359
Size: 207 288 3130 844
Fragments arranged by size:
3130 844 288 207
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 448 480 3986 4018 448
Size: 32 3506 32 899
Fragments arranged by size:
3506 899 32 32
Tth111I GACn'n_nGTC
Cuts at: 682 2266 682
Size: 1584 2885
XbaI T'CTAG_A
Cuts at: 1500 1500
Size: 4469
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 429 621 1032 1964 429
Size: 192 411 932 2934
Fragments arranged by size:
2934 932 411 192
XmaI C'CCGG_G
Cuts at: 1257 1489 1257
Size: 232 4237
ZraI GAC'GTC
Cuts at: 1041 1041
Size: 4469
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AlfI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI BsiWI BsmI BspEI
BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI