Restriction map for
pF1KB5194
(Circular) (Six-base) MAPSORT of: pF1KB5194.seq Check: 8125 from: 1 to: 3602
pF1KB5194 3602 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 640 640
Size: 3602
Acc65I G'GTAC_C
Cuts at: 618 618
Size: 3602
AclI AA'CG_TT
Cuts at: 3442 3442
Size: 3602
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 344 566 1427 1859 2606 2874 344
Size: 222 861 432 747 268 1072
Fragments arranged by size:
1072 861 747 432 268 222
AflIII A'CryG_T
Cuts at: 954 2058 3035 954
Size: 1104 977 1521
Fragments arranged by size:
1521 1104 977
AgeI A'CCGG_T
Cuts at: 1990 1990
Size: 3602
AlwNI CAG_nnn'CTG
Cuts at: 907 2474 907
Size: 1567 2035
ApaLI G'TGCA_C
Cuts at: 291 2372 291
Size: 2081 1521
ApoI r'AATT_y
Cuts at: 379 606 2955 3211 379
Size: 227 2349 256 770
Fragments arranged by size:
2349 770 256 227
AseI AT'TA_AT
Cuts at: 20 20
Size: 3602
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3602
AvaI C'yCGr_G
Cuts at: 622 622
Size: 3602
BamHI G'GATC_C
Cuts at: 627 627
Size: 3602
BanI G'GyrC_C
Cuts at: 618 1032 1280 1315 618
Size: 414 248 35 2905
Fragments arranged by size:
2905 414 248 35
BanII G_rGCy'C
Cuts at: 616 1646 616
Size: 1030 2572
BbeI G_GCGC'C
Cuts at: 1036 1284 1036
Size: 248 3354
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3568
BciVI GTATCCnnnnn_n'
Cuts at: 1500 2261 3602 1500
Size: 761 1341 1500
Fragments arranged by size:
1500 1341 761
BclI T'GATC_A
Cuts at: 485 1122 485
Size: 637 2965
BglII A'GATC_T
Cuts at: 1117 3154 1117
Size: 2037 1565
BlpI GC'TnA_GC
Cuts at: 714 714
Size: 3602
Bme1580I G_kGCm'C
Cuts at: 295 1227 1320 2376 295
Size: 932 93 1056 1521
Fragments arranged by size:
1521 1056 932 93
BmrI ACTGGGnnnn_n'
Cuts at: 232 814 895 1230 3374 232
Size: 582 81 335 2144 460
Fragments arranged by size:
2144 582 460 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1969 1969
Size: 3602
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 1657 2149 2447 778
Size: 879 492 298 1933
Fragments arranged by size:
1933 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3306 3306
Size: 3602
BsaAI yAC'GTr
Cuts at: 955 955
Size: 3602
BsaBI GATnn'nnATC
Cuts at: 6 1116 3153 6
Size: 1110 2037 455
Fragments arranged by size:
2037 1110 455
BsaHI Gr'CG_yC
Cuts at: 1033 1281 1983 3479 1033
Size: 248 702 1496 1156
Fragments arranged by size:
1496 1156 702 248
BsaWI w'CCGG_w
Cuts at: 791 1312 1990 2264 2411 2822 791
Size: 521 678 274 147 411 1571
Fragments arranged by size:
1571 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 305 308 520 565 305
Size: 3 212 45 3342
Fragments arranged by size:
3342 212 45 3
BseYI C'CCAG_C
Cuts at: 1027 1785 2362 2858 1027
Size: 758 577 496 1771
Fragments arranged by size:
1771 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1144 1190 2398 104
Size: 1040 46 1208 1308
Fragments arranged by size:
1308 1208 1040 46
BsiHKAI G_wGCw'C
Cuts at: 295 616 1394 1584 2376 295
Size: 321 778 190 792 1521
Fragments arranged by size:
1521 792 778 321 190
BsmI GAATG_Cn'
Cuts at: 264 264
Size: 3602
Bsp1286I G_dGCh'C
Cuts at: 295 616 1227 1320 1394 1584 1646 2376 295
Size: 321 611 93 74 190 62 730 1521
Fragments arranged by size:
1521 730 611 321 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2822 2822
Size: 3602
BspHI T'CATG_A
Cuts at: 2778 2778
Size: 3602
BspMI ACCTGCnnnn'nnnn_
Cuts at: 652 866 1168 1549 652
Size: 214 302 381 2705
Fragments arranged by size:
2705 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1894 1948 3432 3597 50
Size: 1844 54 1484 165 55
Fragments arranged by size:
1844 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1514 1514
Size: 3602
BsrFI r'CCGG_y
Cuts at: 1600 1781 1990 1600
Size: 181 209 3212
Fragments arranged by size:
3212 209 181
BssSI C'ACGA_G
Cuts at: 1873 2231 1873
Size: 358 3244
BstAPI GCAn_nnn'nTGC
Cuts at: 979 979
Size: 3602
BstBI TT'CG_AA
Cuts at: 1963 1963
Size: 3602
BstYI r'GATC_y
Cuts at: 1 627 1094 1117 1452 1698 2710 2796 2808
Size: 626 467 23 335 246 1012 86 12
Cuts at: 2808 3154 1
Size: 346 449
Fragments arranged by size:
1012 626 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 503 503
Size: 3602
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1552 1733 1896 1552
Size: 181 163 3258
Fragments arranged by size:
3258 181 163
BtsI GCAGTG_nn'
Cuts at: 845 845
Size: 3602
DraI TTT'AAA
Cuts at: 601 601
Size: 3602
DrdI GACnn_nn'nnGTC
Cuts at: 1308 2166 1308
Size: 858 2744
EaeI y'GGCC_r
Cuts at: 1187 1361 1752 1779 3124 1187
Size: 174 391 27 1345 1665
Fragments arranged by size:
1665 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1187 1187
Size: 3602
EarI CTCTTCn'nnn_
Cuts at: 542 584 1625 1835 542
Size: 42 1041 210 2309
Fragments arranged by size:
2309 1041 210 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1810 2120 2266 3412 1810
Size: 310 146 1146 2000
Fragments arranged by size:
2000 1146 310 146
EcoICRI GAG'CTC
Cuts at: 614 614
Size: 3602
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 344 566 1427 1859 2606 2874 344
Size: 222 861 432 747 268 1072
Fragments arranged by size:
1072 861 747 432 268 222
EcoO109I rG'GnC_Cy
Cuts at: 741 741
Size: 3602
EcoRI G'AATT_C
Cuts at: 379 606 379
Size: 227 3375
EcoRV GAT'ATC
Cuts at: 360 360
Size: 3602
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 421 469 1621 421
Size: 48 1152 2402
Fragments arranged by size:
2402 1152 48
FspI TGC'GCA
Cuts at: 1383 1383
Size: 3602
HaeII r_GCGC'y
Cuts at: 1036 1284 2306 3285 1036
Size: 248 1022 979 1353
Fragments arranged by size:
1353 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1444 1474 1476 1506 1444
Size: 30 2 30 3540
Fragments arranged by size:
3540 30 30 2
HincII GTy'rAC
Cuts at: 393 641 393
Size: 248 3354
KasI G'GCGC_C
Cuts at: 1032 1280 1032
Size: 248 3354
KpnI G_GTAC'C
Cuts at: 622 622
Size: 3602
MluI A'CGCG_T
Cuts at: 3035 3035
Size: 3602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 179 2273 2457 179
Size: 2094 184 1324
Fragments arranged by size:
2094 1324 184
MscI TGG'CCA
Cuts at: 1363 1363
Size: 3602
MslI CAynn'nnrTG
Cuts at: 305 1591 1718 305
Size: 1286 127 2189
Fragments arranged by size:
2189 1286 127
MspA1I CmG'CkG
Cuts at: 713 1027 1387 2400 2645 713
Size: 314 360 1013 245 1670
Fragments arranged by size:
1670 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1783 1783
Size: 3602
NarI GG'CG_CC
Cuts at: 1033 1281 1033
Size: 248 3354
NgoMIV G'CCGG_C
Cuts at: 1781 1781
Size: 3602
NspI r_CATG'y
Cuts at: 467 655 1155 2062 467
Size: 188 500 907 2007
Fragments arranged by size:
2007 907 500 188
PciI A'CATG_T
Cuts at: 2058 2058
Size: 3602
PmeI GTTT'AAAC
Cuts at: 601 601
Size: 3602
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2741 2773 2741
Size: 32 3570
PstI C_TGCA'G
Cuts at: 649 1334 649
Size: 685 2917
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3602
PvuII CAG'CTG
Cuts at: 1027 1387 1027
Size: 360 3242
RsrII CG'GwC_CG
Cuts at: 1797 1797
Size: 3602
SacI G_AGCT'C
Cuts at: 616 616
Size: 3602
SalI G'TCGA_C
Cuts at: 639 639
Size: 3602
SapI GCTCTTCn'nnn_
Cuts at: 1625 1835 1625
Size: 210 3392
SbfI CC_TGCA'GG
Cuts at: 649 649
Size: 3602
ScaI AGT'ACT
Cuts at: 3119 3119
Size: 3602
SfcI C'TryA_G
Cuts at: 33 645 1330 2323 2514 33
Size: 612 685 993 191 1121
Fragments arranged by size:
1121 993 685 612 191
SfoI GGC'GCC
Cuts at: 1034 1282 1034
Size: 248 3354
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3602
SmaI CCC'GGG
Cuts at: 624 624
Size: 3602
SmlI C'TyrA_G
Cuts at: 757 1672 2164 2426 757
Size: 915 492 262 1933
Fragments arranged by size:
1933 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3602
SphI G_CATG'C
Cuts at: 655 1155 655
Size: 500 3102
SspI AAT'ATT
Cuts at: 269 269
Size: 3602
StyI C'CwwG_G
Cuts at: 396 736 1090 396
Size: 340 354 2908
Fragments arranged by size:
2908 354 340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 310 1185 1318 1777 1986 310
Size: 875 133 459 209 1926
Fragments arranged by size:
1926 875 459 209 133
TatI w'GTAC_w
Cuts at: 3117 3117
Size: 3602
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3119 3151 3119
Size: 32 3570
Tth111I GACn'n_nGTC
Cuts at: 1399 1399
Size: 3602
XbaI T'CTAG_A
Cuts at: 633 633
Size: 3602
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1097 1097
Size: 3602
XmaI C'CCGG_G
Cuts at: 622 622
Size: 3602
XmnI GAAnn'nnTTC
Cuts at: 416 416
Size: 3602
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I BtgI ClaI CspCI
DraIII EcoNI FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI