Restriction map for
pF1KB5190
(Circular) (Six-base) MAPSORT of: pF1KB5190.seq Check: 7075 from: 1 to: 4877
pF1KB5190 4877 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1843 1843
Size: 4877
AccI GT'mk_AC
Cuts at: 1915 1915
Size: 4877
Acc65I G'GTAC_C
Cuts at: 1893 1893
Size: 4877
AclI AA'CG_TT
Cuts at: 4717 4717
Size: 4877
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 590 929 1273 1535 1712 1804 2702 3134 3487
Size: 339 344 262 177 92 898 432 353
Cuts at: 3487 4149 590
Size: 662 1318
Fragments arranged by size:
1318 898 662 432 353 344 339 262
177 92
AflIII A'CryG_T
Cuts at: 2229 4029 4310 2229
Size: 1800 281 2796
Fragments arranged by size:
2796 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 525 525
Size: 4877
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1525 1557 1525
Size: 32 4845
AlwNI CAG_nnn'CTG
Cuts at: 499 2182 3620 499
Size: 1683 1438 1756
Fragments arranged by size:
1756 1683 1438
ApaLI G'TGCA_C
Cuts at: 3715 3715
Size: 4877
ApoI r'AATT_y
Cuts at: 624 758 1322 1881 4230 4486 624
Size: 134 564 559 2349 256 1015
Fragments arranged by size:
2349 1015 564 559 256 134
AseI AT'TA_AT
Cuts at: 20 20
Size: 4877
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4877
AvaI C'yCGr_G
Cuts at: 892 1675 1897 892
Size: 783 222 3872
Fragments arranged by size:
3872 783 222
BamHI G'GATC_C
Cuts at: 1902 1902
Size: 4877
BanI G'GyrC_C
Cuts at: 954 1183 1225 1831 1893 2307 2555 2590 954
Size: 229 42 606 62 414 248 35 3241
Fragments arranged by size:
3241 606 414 248 229 62 42 35
BanII G_rGCy'C
Cuts at: 651 1891 2921 651
Size: 1240 1030 2607
Fragments arranged by size:
2607 1240 1030
BbeI G_GCGC'C
Cuts at: 1229 2311 2559 1229
Size: 1082 248 3547
Fragments arranged by size:
3547 1082 248
BbsI GAAGACnn'nnnn_
Cuts at: 557 917 1037 1463 557
Size: 360 120 426 3971
Fragments arranged by size:
3971 426 360 120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4843
BciVI GTATCCnnnnn_n'
Cuts at: 248 810 2775 3831 4877 248
Size: 562 1965 1056 1046 248
Fragments arranged by size:
1965 1056 1046 562 248
BclI T'GATC_A
Cuts at: 2397 2397
Size: 4877
BglII A'GATC_T
Cuts at: 2392 4429 2392
Size: 2037 2840
BlpI GC'TnA_GC
Cuts at: 513 1989 513
Size: 1476 3401
Bme1580I G_kGCm'C
Cuts at: 2502 2595 3719 2502
Size: 93 1124 3660
Fragments arranged by size:
3660 1124 93
BmgBI CAC'GTC
Cuts at: 637 637
Size: 4877
BmrI ACTGGGnnnn_n'
Cuts at: 119 487 2089 2170 2505 4649 119
Size: 368 1602 81 335 2144 347
Fragments arranged by size:
2144 1602 368 347 335 81
BmtI G_CTAG'C
Cuts at: 521 521
Size: 4877
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 858 3244 858
Size: 2386 2491
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1089 1318 1428 1089
Size: 229 110 4538
Fragments arranged by size:
4538 229 110
Bpu10I CC'TnA_GC
Cuts at: 1163 1163
Size: 4877
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2053 2932 3646 3944 2053
Size: 879 714 298 2986
Fragments arranged by size:
2986 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4581 4581
Size: 4877
BsaAI yAC'GTr
Cuts at: 2230 2230
Size: 4877
BsaBI GATnn'nnATC
Cuts at: 6 2391 4428 6
Size: 2385 2037 455
Fragments arranged by size:
2385 2037 455
BsaHI Gr'CG_yC
Cuts at: 1226 2308 2556 3258 4754 1226
Size: 1082 248 702 1496 1349
Fragments arranged by size:
1496 1349 1082 702 248
BsaWI w'CCGG_w
Cuts at: 2066 2587 3265 3676 3823 4097 2066
Size: 521 678 411 147 274 2846
Fragments arranged by size:
2846 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 156 337 340 487 942 156
Size: 181 3 147 455 4091
Fragments arranged by size:
4091 455 181 147 3
BseYI C'CCAG_C
Cuts at: 334 427 640 1118 2302 3060 3725 4133 334
Size: 93 213 478 1184 758 665 408 1078
Fragments arranged by size:
1184 1078 758 665 478 408 213 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 617 713 617
Size: 96 4781
BsiEI CG_ry'CG
Cuts at: 104 151 352 2419 2465 3695 104
Size: 47 201 2067 46 1230 1286
Fragments arranged by size:
2067 1286 1230 201 47 46
BsiHKAI G_wGCw'C
Cuts at: 1891 2669 2859 3719 1891
Size: 778 190 860 3049
Fragments arranged by size:
3049 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 954 954
Size: 4877
Bsp1286I G_dGCh'C
Cuts at: 651 1891 2502 2595 2669 2859 2921 3719 651
Size: 1240 611 93 74 190 62 798 1809
Fragments arranged by size:
1809 1240 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 3309 3309
Size: 4877
BspMI ACCTGCnnnn'nnnn_
Cuts at: 639 1843 1927 2141 2443 2824 639
Size: 1204 84 214 302 381 2692
Fragments arranged by size:
2692 1204 381 302 214 84
BsrBI CCG'CTC
Cuts at: 50 1146 3169 3223 4707 4872 50
Size: 1096 2023 54 1484 165 55
Fragments arranged by size:
2023 1484 1096 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1254 1589 2789 1254
Size: 335 1200 3342
Fragments arranged by size:
3342 1200 335
BsrFI r'CCGG_y
Cuts at: 2875 3056 2875
Size: 181 4696
BssSI C'ACGA_G
Cuts at: 1384 3148 3856 1384
Size: 1764 708 2405
Fragments arranged by size:
2405 1764 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2254 2254
Size: 4877
BstBI TT'CG_AA
Cuts at: 3238 3238
Size: 4877
BstXI CCAn_nnnn'nTGG
Cuts at: 847 847
Size: 4877
BstYI r'GATC_y
Cuts at: 1 1679 1902 2369 2392 2727 2973 3279 3291
Size: 1678 223 467 23 335 246 306 12
Cuts at: 3291 3377 4429 1
Size: 86 1052 449
Fragments arranged by size:
1678 1052 467 449 335 306 246 223
86 23 12
BtgI C'CryG_G
Cuts at: 974 974
Size: 4877
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2827 3008 3171 2827
Size: 181 163 4533
Fragments arranged by size:
4533 181 163
BtsI GCAGTG_nn'
Cuts at: 1365 1625 2120 1365
Size: 260 495 4122
Fragments arranged by size:
4122 495 260
DraI TTT'AAA
Cuts at: 381 1876 381
Size: 1495 3382
DrdI GACnn_nn'nnGTC
Cuts at: 2583 3927 2583
Size: 1344 3533
EaeI y'GGCC_r
Cuts at: 977 1634 2462 2636 3027 3054 4399 977
Size: 657 828 174 391 27 1345 1455
Fragments arranged by size:
1455 1345 828 657 391 174 27
EagI C'GGCC_G
Cuts at: 2462 2462
Size: 4877
EarI CTCTTCn'nnn_
Cuts at: 267 855 1136 1289 1292 1676 2900 3110 267
Size: 588 281 153 3 384 1224 210 2034
Fragments arranged by size:
2034 1224 588 384 281 210 153 3
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 230 3085 3827 3973 4687 230
Size: 2855 742 146 714 420
Fragments arranged by size:
2855 742 714 420 146
EcoICRI GAG'CTC
Cuts at: 1889 1889
Size: 4877
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 590 929 1089 1273 1318 1428 1535 1712 1804
Size: 339 160 184 45 110 107 177 92
Cuts at: 1804 2702 3134 3487 4149 590
Size: 898 432 353 662 1318
Fragments arranged by size:
1318 898 662 432 353 339 184 177
160 110 107 92 45
EcoNI CCTnn'n_nnAGG
Cuts at: 1576 1576
Size: 4877
EcoO109I rG'GnC_Cy
Cuts at: 2016 2016
Size: 4877
EcoRI G'AATT_C
Cuts at: 1881 1881
Size: 4877
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2896 2896
Size: 4877
FspI TGC'GCA
Cuts at: 1601 2658 1601
Size: 1057 3820
HaeII r_GCGC'y
Cuts at: 1229 2311 2559 3789 4560 1229
Size: 1082 248 1230 771 1546
Fragments arranged by size:
1546 1230 1082 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1018 1047 1050 1079 1288 1320 2719 2749 2751
Size: 29 3 29 209 32 1399 30 2
Cuts at: 2751 2781 1018
Size: 30 3114
Fragments arranged by size:
3114 1399 209 32 30 30 29 29
3 2
HincII GTy'rAC
Cuts at: 1916 1916
Size: 4877
KasI G'GCGC_C
Cuts at: 1225 2307 2555 1225
Size: 1082 248 3547
Fragments arranged by size:
3547 1082 248
KpnI G_GTAC'C
Cuts at: 1897 1897
Size: 4877
MluI A'CGCG_T
Cuts at: 4310 4310
Size: 4877
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1051 3636 3820 1051
Size: 2585 184 2108
Fragments arranged by size:
2585 2108 184
MscI TGG'CCA
Cuts at: 1636 2638 1636
Size: 1002 3875
MslI CAynn'nnrTG
Cuts at: 845 1004 1346 1552 2866 2993 845
Size: 159 342 206 1314 127 2729
Fragments arranged by size:
2729 1314 342 206 159 127
MspA1I CmG'CkG
Cuts at: 1850 1988 2302 2662 3446 3691 1850
Size: 138 314 360 784 245 3036
Fragments arranged by size:
3036 784 360 314 245 138
NaeI GCC'GGC
Cuts at: 3058 3058
Size: 4877
NarI GG'CG_CC
Cuts at: 1226 2308 2556 1226
Size: 1082 248 3547
Fragments arranged by size:
3547 1082 248
NcoI C'CATG_G
Cuts at: 974 974
Size: 4877
NgoMIV G'CCGG_C
Cuts at: 3056 3056
Size: 4877
NheI G'CTAG_C
Cuts at: 517 517
Size: 4877
NspI r_CATG'y
Cuts at: 1857 1930 2430 4033 1857
Size: 73 500 1603 2701
Fragments arranged by size:
2701 1603 500 73
PciI A'CATG_T
Cuts at: 4029 4029
Size: 4877
PflMI CCAn_nnn'nTGG
Cuts at: 1584 1584
Size: 4877
PmeI GTTT'AAAC
Cuts at: 1876 1876
Size: 4877
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3323 3355 3323
Size: 32 4845
PstI C_TGCA'G
Cuts at: 504 1924 2609 504
Size: 1420 685 2772
Fragments arranged by size:
2772 1420 685
PvuI CG_AT'CG
Cuts at: 104 151 104
Size: 47 4830
PvuII CAG'CTG
Cuts at: 2302 2662 2302
Size: 360 4517
RsrII CG'GwC_CG
Cuts at: 3072 3072
Size: 4877
SacI G_AGCT'C
Cuts at: 1891 1891
Size: 4877
SalI G'TCGA_C
Cuts at: 1914 1914
Size: 4877
SapI GCTCTTCn'nnn_
Cuts at: 1136 1289 1676 2900 3110 1136
Size: 153 387 1224 210 2903
Fragments arranged by size:
2903 1224 387 210 153
SbfI CC_TGCA'GG
Cuts at: 1924 1924
Size: 4877
ScaI AGT'ACT
Cuts at: 801 4394 801
Size: 3593 1284
SfcI C'TryA_G
Cuts at: 33 500 1737 1920 2605 3573 3764 33
Size: 467 1237 183 685 968 191 1146
Fragments arranged by size:
1237 1146 968 685 467 191 183
SfoI GGC'GCC
Cuts at: 1227 2309 2557 1227
Size: 1082 248 3547
Fragments arranged by size:
3547 1082 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4877
SmaI CCC'GGG
Cuts at: 1899 1899
Size: 4877
SmlI C'TyrA_G
Cuts at: 2032 2947 3661 3923 2032
Size: 915 714 262 2986
Fragments arranged by size:
2986 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4877
SphI G_CATG'C
Cuts at: 1930 2430 1930
Size: 500 4377
SspI AAT'ATT
Cuts at: 213 213
Size: 4877
StyI C'CwwG_G
Cuts at: 974 1327 2011 2365 974
Size: 353 684 354 3486
Fragments arranged by size:
3486 684 354 353
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2460 2593 3052 3261 2460
Size: 133 459 209 4076
Fragments arranged by size:
4076 459 209 133
TatI w'GTAC_w
Cuts at: 251 799 4392 251
Size: 548 3593 736
Fragments arranged by size:
3593 736 548
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 787 819 987 1019 4394 4426 787
Size: 32 168 32 3375 32 1238
Fragments arranged by size:
3375 1238 168 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 493 2674 493
Size: 2181 2696
XbaI T'CTAG_A
Cuts at: 1908 1908
Size: 4877
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1527 2372 1527
Size: 845 4032
XmaI C'CCGG_G
Cuts at: 1897 1897
Size: 4877
XmnI GAAnn'nnTTC
Cuts at: 1271 1271
Size: 4877
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PciI PflMI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BsaXI BsiWI BsmI
BspEI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI