Restriction map for
pF1KB5188
(Circular) (Six-base) MAPSORT of: pF1KB5188.seq Check: 671 from: 1 to: 4379
pF1KB5188 4379 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AatII G_ACGT'C
Cuts at: 1024 1024
Size: 4379
AccI GT'mk_AC
Cuts at: 1184 1417 1184
Size: 233 4146
Acc65I G'GTAC_C
Cuts at: 1395 1395
Size: 4379
AclI AA'CG_TT
Cuts at: 4219 4219
Size: 4379
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2204 2636 2989 3651 2204
Size: 432 353 662 2932
Fragments arranged by size:
2932 662 432 353
AflIII A'CryG_T
Cuts at: 1731 3531 3812 1731
Size: 1800 281 2298
Fragments arranged by size:
2298 1800 281
AleI CACnn'nnGTG
Cuts at: 799 844 799
Size: 45 4334
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 909 922 1075 909
Size: 13 153 4213
Fragments arranged by size:
4213 153 13
AlwNI CAG_nnn'CTG
Cuts at: 170 239 922 1684 3122 170
Size: 69 683 762 1438 1427
Fragments arranged by size:
1438 1427 762 683 69
ApaI G_GGCC'C
Cuts at: 822 822
Size: 4379
ApaLI G'TGCA_C
Cuts at: 660 3217 660
Size: 2557 1822
ApoI r'AATT_y
Cuts at: 1383 3732 3988 1383
Size: 2349 256 1774
Fragments arranged by size:
2349 1774 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4379
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4379
AvaI C'yCGr_G
Cuts at: 1399 1399
Size: 4379
BamHI G'GATC_C
Cuts at: 1004 1404 1004
Size: 400 3979
BanI G'GyrC_C
Cuts at: 457 1122 1369 1395 1809 2057 2092 457
Size: 665 247 26 414 248 35 2744
Fragments arranged by size:
2744 665 414 248 247 35 26
BanII G_rGCy'C
Cuts at: 277 822 1393 2423 277
Size: 545 571 1030 2233
Fragments arranged by size:
2233 1030 571 545
BbeI G_GCGC'C
Cuts at: 1813 2061 1813
Size: 248 4131
BbsI GAAGACnn'nnnn_
Cuts at: 194 1098 194
Size: 904 3475
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1071 1105 1071
Size: 34 4345
BciVI GTATCCnnnnn_n'
Cuts at: 2277 3333 4379 2277
Size: 1056 1046 2277
Fragments arranged by size:
2277 1056 1046
BclI T'GATC_A
Cuts at: 1899 1899
Size: 4379
BglI GCCn_nnn'nGGC
Cuts at: 473 473
Size: 4379
BglII A'GATC_T
Cuts at: 579 1894 3931 579
Size: 1315 2037 1027
Fragments arranged by size:
2037 1315 1027
BlpI GC'TnA_GC
Cuts at: 276 1491 276
Size: 1215 3164
Bme1580I G_kGCm'C
Cuts at: 664 822 1125 2004 2097 3221 664
Size: 158 303 879 93 1124 1822
Fragments arranged by size:
1822 1124 879 303 158 93
BmrI ACTGGGnnnn_n'
Cuts at: 1143 1591 1672 2007 4151 1143
Size: 448 81 335 2144 1371
Fragments arranged by size:
2144 1371 448 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 291 296 2746 291
Size: 5 2450 1924
Fragments arranged by size:
2450 1924 5
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 261 261
Size: 4379
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1555 2434 3148 3446 1555
Size: 879 714 298 2488
Fragments arranged by size:
2488 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 646 4083 646
Size: 3437 942
BsaAI yAC'GTr
Cuts at: 999 1732 999
Size: 733 3646
BsaBI GATnn'nnATC
Cuts at: 6 1893 3930 6
Size: 1887 2037 455
Fragments arranged by size:
2037 1887 455
BsaHI Gr'CG_yC
Cuts at: 1021 1810 2058 2760 4256 1021
Size: 789 248 702 1496 1144
Fragments arranged by size:
1496 1144 789 702 248
BsaWI w'CCGG_w
Cuts at: 776 1568 2089 2767 3178 3325 3599 776
Size: 792 521 678 411 147 274 1556
Fragments arranged by size:
1556 792 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 643 673 872 902 643
Size: 30 199 30 4120
Fragments arranged by size:
4120 199 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 395 574 1363 395
Size: 179 789 3411
Fragments arranged by size:
3411 789 179
BseYI C'CCAG_C
Cuts at: 1043 1118 1804 2562 3227 3635 1043
Size: 75 686 758 665 408 1787
Fragments arranged by size:
1787 758 686 665 408 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 905 511
Size: 394 3985
BsiEI CG_ry'CG
Cuts at: 104 302 509 1921 1967 3197 104
Size: 198 207 1412 46 1230 1286
Fragments arranged by size:
1412 1286 1230 207 198 46
BsiHKAI G_wGCw'C
Cuts at: 277 664 1256 1393 2171 2361 3221 277
Size: 387 592 137 778 190 860 1435
Fragments arranged by size:
1435 860 778 592 387 190 137
BsmBI CGTCTCn'nnnn_
Cuts at: 1255 1255
Size: 4379
Bsp1286I G_dGCh'C
Cuts at: 277 664 822 1125 1256 1393 2004 2097 2171
Size: 387 158 303 131 137 611 93 74
Cuts at: 2171 2361 2423 3221 277
Size: 190 62 798 1435
Fragments arranged by size:
1435 798 611 387 303 190 158 137
131 93 74 62
BspEI T'CCGG_A
Cuts at: 776 776
Size: 4379
BspHI T'CATG_A
Cuts at: 608 2811 608
Size: 2203 2176
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1429 1643 1945 2326 1429
Size: 214 302 381 3482
Fragments arranged by size:
3482 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2671 2725 4209 4374 50
Size: 2621 54 1484 165 55
Fragments arranged by size:
2621 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 636 2291 636
Size: 1655 2724
BsrFI r'CCGG_y
Cuts at: 301 2377 2558 301
Size: 2076 181 2122
Fragments arranged by size:
2122 2076 181
BsrGI T'GTAC_A
Cuts at: 1244 1244
Size: 4379
BssSI C'ACGA_G
Cuts at: 2650 3358 2650
Size: 708 3671
BstAPI GCAn_nnn'nTGC
Cuts at: 170 464 1756 170
Size: 294 1292 2793
Fragments arranged by size:
2793 1292 294
BstBI TT'CG_AA
Cuts at: 2740 2740
Size: 4379
BstEII G'GTnAC_C
Cuts at: 1074 1074
Size: 4379
BstXI CCAn_nnnn'nTGG
Cuts at: 114 1101 1209 114
Size: 987 108 3284
Fragments arranged by size:
3284 987 108
BstYI r'GATC_y
Cuts at: 1 579 1004 1404 1871 1894 2229 2475 2781
Size: 578 425 400 467 23 335 246 306
Cuts at: 2781 2793 2879 3931 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 578 467 449 425 400 335 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 491 677 722 491
Size: 186 45 4148
Fragments arranged by size:
4148 186 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1074 2329 2510 2673 1074
Size: 1255 181 163 2780
Fragments arranged by size:
2780 1255 181 163
BtsI GCAGTG_nn'
Cuts at: 893 1622 893
Size: 729 3650
DraI TTT'AAA
Cuts at: 1378 1378
Size: 4379
DrdI GACnn_nn'nnGTC
Cuts at: 1152 2085 3429 1152
Size: 933 1344 2102
Fragments arranged by size:
2102 1344 933
EaeI y'GGCC_r
Cuts at: 674 725 743 791 1199 1964 2138 2529 2556
Size: 51 18 48 408 765 174 391 27
Cuts at: 2556 3901 674
Size: 1345 1152
Fragments arranged by size:
1345 1152 765 408 391 174 51 48
27 18
EagI C'GGCC_G
Cuts at: 1964 1964
Size: 4379
EarI CTCTTCn'nnn_
Cuts at: 768 2402 2612 768
Size: 1634 210 2535
Fragments arranged by size:
2535 1634 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2587 3329 3475 4189 2587
Size: 742 146 714 2777
Fragments arranged by size:
2777 742 714 146
EcoICRI GAG'CTC
Cuts at: 275 1391 275
Size: 1116 3263
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 261 2204 2636 2989 3651 261
Size: 1943 432 353 662 989
Fragments arranged by size:
1943 989 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 819 1518 819
Size: 699 3680
EcoRI G'AATT_C
Cuts at: 1383 1383
Size: 4379
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 211 2398 211
Size: 2187 2192
FspI TGC'GCA
Cuts at: 2160 2160
Size: 4379
HaeII r_GCGC'y
Cuts at: 240 1813 2061 3291 4062 240
Size: 1573 248 1230 771 557
Fragments arranged by size:
1573 1230 771 557 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2221 2251 2253 2283 2221
Size: 30 2 30 4317
Fragments arranged by size:
4317 30 30 2
HincII GTy'rAC
Cuts at: 1418 1418
Size: 4379
KasI G'GCGC_C
Cuts at: 1809 2057 1809
Size: 248 4131
KpnI G_GTAC'C
Cuts at: 1399 1399
Size: 4379
MluI A'CGCG_T
Cuts at: 3812 3812
Size: 4379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3138 3322 3138
Size: 184 4195
MscI TGG'CCA
Cuts at: 727 745 793 2140 727
Size: 18 48 1347 2966
Fragments arranged by size:
2966 1347 48 18
MslI CAynn'nnrTG
Cuts at: 112 439 676 799 844 2368 2495 112
Size: 327 237 123 45 1524 127 1996
Fragments arranged by size:
1996 1524 327 237 127 123 45
MspA1I CmG'CkG
Cuts at: 167 1143 1226 1490 1804 2164 2948 3193 167
Size: 976 83 264 314 360 784 245 1353
Fragments arranged by size:
1353 976 784 360 314 264 245 83
NaeI GCC'GGC
Cuts at: 2560 2560
Size: 4379
NarI GG'CG_CC
Cuts at: 1810 2058 1810
Size: 248 4131
NcoI C'CATG_G
Cuts at: 677 722 677
Size: 45 4334
NgoMIV G'CCGG_C
Cuts at: 2558 2558
Size: 4379
NspI r_CATG'y
Cuts at: 603 1432 1932 3535 603
Size: 829 500 1603 1447
Fragments arranged by size:
1603 1447 829 500
PasI CC'CwG_GG
Cuts at: 1305 1305
Size: 4379
PciI A'CATG_T
Cuts at: 3531 3531
Size: 4379
PflMI CCAn_nnn'nTGG
Cuts at: 624 624
Size: 4379
PfoI T'CCnGG_A
Cuts at: 219 219
Size: 4379
PmeI GTTT'AAAC
Cuts at: 1378 1378
Size: 4379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2825 2857 2825
Size: 32 4347
PspOMI G'GGCC_C
Cuts at: 818 818
Size: 4379
PstI C_TGCA'G
Cuts at: 1013 1302 1426 2111 1013
Size: 289 124 685 3281
Fragments arranged by size:
3281 685 289 124
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4379
PvuII CAG'CTG
Cuts at: 1143 1226 1804 2164 1143
Size: 83 578 360 3358
Fragments arranged by size:
3358 578 360 83
RsrII CG'GwC_CG
Cuts at: 2574 2574
Size: 4379
SacI G_AGCT'C
Cuts at: 277 1393 277
Size: 1116 3263
SalI G'TCGA_C
Cuts at: 1416 1416
Size: 4379
SapI GCTCTTCn'nnn_
Cuts at: 2402 2612 2402
Size: 210 4169
SbfI CC_TGCA'GG
Cuts at: 1426 1426
Size: 4379
ScaI AGT'ACT
Cuts at: 3896 3896
Size: 4379
SfcI C'TryA_G
Cuts at: 33 1009 1138 1298 1422 2107 3075 3266 33
Size: 976 129 160 124 685 968 191 1146
Fragments arranged by size:
1146 976 968 685 191 160 129 124
SfoI GGC'GCC
Cuts at: 1811 2059 1811
Size: 248 4131
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4379
SmaI CCC'GGG
Cuts at: 1401 1401
Size: 4379
SmlI C'TyrA_G
Cuts at: 1534 2449 3163 3425 1534
Size: 915 714 262 2488
Fragments arranged by size:
2488 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4379
SphI G_CATG'C
Cuts at: 603 1432 1932 603
Size: 829 500 3050
Fragments arranged by size:
3050 829 500
StyI C'CwwG_G
Cuts at: 677 722 1513 1867 677
Size: 45 791 354 3189
Fragments arranged by size:
3189 791 354 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 252 1962 2095 2554 2763 252
Size: 1710 133 459 209 1868
Fragments arranged by size:
1868 1710 459 209 133
TatI w'GTAC_w
Cuts at: 1244 3894 1244
Size: 2650 1729
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 643 675 872 904 3896 3928 643
Size: 32 197 32 2992 32 1094
Fragments arranged by size:
2992 1094 197 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2176 2176
Size: 4379
XbaI T'CTAG_A
Cuts at: 1410 1410
Size: 4379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 468 1874 468
Size: 1406 2973
XmaI C'CCGG_G
Cuts at: 1399 1399
Size: 4379
ZraI GAC'GTC
Cuts at: 1022 1022
Size: 4379
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI BsmI
BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI