Restriction map for
pF1KB5185
(Circular) (Six-base) MAPSORT of: pF1KB5185.seq Check: 757 from: 1 to: 4307
pF1KB5185 4307 bp
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 942 942
Size: 4307
AccI GT'mk_AC
Cuts at: 743 1345 743
Size: 602 3705
Acc65I G'GTAC_C
Cuts at: 125 1323 125
Size: 1198 3109
AclI AA'CG_TT
Cuts at: 4147 4147
Size: 4307
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 1261 2132 2564 3311 3579 364
Size: 897 871 432 747 268 1092
Fragments arranged by size:
1092 897 871 747 432 268
AflIII A'CryG_T
Cuts at: 1659 2763 3740 1659
Size: 1104 977 2226
Fragments arranged by size:
2226 1104 977
AgeI A'CCGG_T
Cuts at: 2695 2695
Size: 4307
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 439 1222 439
Size: 783 3524
AlwNI CAG_nnn'CTG
Cuts at: 699 728 831 975 1612 3179 699
Size: 29 103 144 637 1567 1827
Fragments arranged by size:
1827 1567 637 144 103 29
ApaI G_GGCC'C
Cuts at: 806 1191 806
Size: 385 3922
ApaLI G'TGCA_C
Cuts at: 3077 3077
Size: 4307
ApoI r'AATT_y
Cuts at: 1311 3660 3916 1311
Size: 2349 256 1702
Fragments arranged by size:
2349 1702 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4307
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4307
AvaI C'yCGr_G
Cuts at: 888 1327 888
Size: 439 3868
BamHI G'GATC_C
Cuts at: 392 1332 392
Size: 940 3367
BanI G'GyrC_C
Cuts at: 125 877 949 1075 1134 1323 1737 1985 2020
Size: 752 72 126 59 189 414 248 35
Cuts at: 2020 125
Size: 2412
Fragments arranged by size:
2412 752 414 248 189 126 72 59
35
BanII G_rGCy'C
Cuts at: 190 806 1191 1321 2351 190
Size: 616 385 130 1030 2146
Fragments arranged by size:
2146 1030 616 385 130
BbeI G_GCGC'C
Cuts at: 1741 1989 1741
Size: 248 4059
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4273
BciVI GTATCCnnnnn_n'
Cuts at: 2205 2966 4307 2205
Size: 761 1341 2205
Fragments arranged by size:
2205 1341 761
BclI T'GATC_A
Cuts at: 1827 1827
Size: 4307
BglII A'GATC_T
Cuts at: 1822 3859 1822
Size: 2037 2270
BlpI GC'TnA_GC
Cuts at: 1419 1419
Size: 4307
Bme1580I G_kGCm'C
Cuts at: 356 806 882 1191 1932 2025 3081 356
Size: 450 76 309 741 93 1056 1582
Fragments arranged by size:
1582 1056 741 450 309 93 76
BmrI ACTGGGnnnn_n'
Cuts at: 1519 1600 1935 4079 1519
Size: 81 335 2144 1747
Fragments arranged by size:
2144 1747 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2674 2674
Size: 4307
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 757 760 808 1297 724
Size: 33 3 48 489 3734
Fragments arranged by size:
3734 489 48 33 3
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 664 926 1483 2362 2854 3152 628
Size: 36 262 557 879 492 298 1783
Fragments arranged by size:
1783 879 557 492 298 262 36
BsaI GGTCTCn'nnnn_
Cuts at: 4011 4011
Size: 4307
BsaAI yAC'GTr
Cuts at: 1660 1660
Size: 4307
BsaBI GATnn'nnATC
Cuts at: 6 1821 3858 6
Size: 1815 2037 455
Fragments arranged by size:
2037 1815 455
BsaHI Gr'CG_yC
Cuts at: 1738 1986 2688 4184 1738
Size: 248 702 1496 1861
Fragments arranged by size:
1861 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1496 2017 2695 2969 3116 3527 1496
Size: 521 678 274 147 411 2276
Fragments arranged by size:
2276 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 524 554 524
Size: 30 4277
BseYI C'CCAG_C
Cuts at: 795 799 1069 1732 2490 3067 3563 795
Size: 4 270 663 758 577 496 1539
Fragments arranged by size:
1539 758 663 577 496 270 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 823 823
Size: 4307
BsiEI CG_ry'CG
Cuts at: 104 1849 1895 3103 104
Size: 1745 46 1208 1308
Fragments arranged by size:
1745 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 776 1321 2099 2289 3081 776
Size: 545 778 190 792 2002
Fragments arranged by size:
2002 792 778 545 190
Bsp1286I G_dGCh'C
Cuts at: 190 356 776 806 882 1191 1321 1932 2025
Size: 166 420 30 76 309 130 611 93
Cuts at: 2025 2099 2289 2351 3081 190
Size: 74 190 62 730 1416
Fragments arranged by size:
1416 730 611 420 309 190 166 130
93 76 74 62 30
BspEI T'CCGG_A
Cuts at: 3527 3527
Size: 4307
BspHI T'CATG_A
Cuts at: 3483 3483
Size: 4307
BspMI ACCTGCnnnn'nnnn_
Cuts at: 107 942 1190 1357 1571 1873 2254 107
Size: 835 248 167 214 302 381 2160
Fragments arranged by size:
2160 835 381 302 248 214 167
BsrBI CCG'CTC
Cuts at: 50 216 366 2599 2653 4137 4302 50
Size: 166 150 2233 54 1484 165 55
Fragments arranged by size:
2233 1484 166 165 150 55 54
BsrDI GCAATG_nn'
Cuts at: 2219 2219
Size: 4307
BsrFI r'CCGG_y
Cuts at: 2305 2486 2695 2305
Size: 181 209 3917
Fragments arranged by size:
3917 209 181
BssSI C'ACGA_G
Cuts at: 470 2578 2936 470
Size: 2108 358 1841
Fragments arranged by size:
2108 1841 358
BstAPI GCAn_nnn'nTGC
Cuts at: 877 1684 877
Size: 807 3500
BstBI TT'CG_AA
Cuts at: 2668 2668
Size: 4307
BstEII G'GTnAC_C
Cuts at: 312 312
Size: 4307
BstXI CCAn_nnnn'nTGG
Cuts at: 412 1275 412
Size: 863 3444
BstYI r'GATC_y
Cuts at: 1 392 459 662 1332 1799 1822 2157 2403
Size: 391 67 203 670 467 23 335 246
Cuts at: 2403 3415 3501 3513 3859 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 670 467 449 391 346 335 246
203 86 67 23 12
Bsu36I CC'TnA_GG
Cuts at: 1288 1288
Size: 4307
BtgI C'CryG_G
Cuts at: 527 905 527
Size: 378 3929
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2257 2438 2601 2257
Size: 181 163 3963
Fragments arranged by size:
3963 181 163
BtsI GCAGTG_nn'
Cuts at: 767 1550 767
Size: 783 3524
DraI TTT'AAA
Cuts at: 1306 1306
Size: 4307
DraIII CAC_nnn'GTG
Cuts at: 1134 1134
Size: 4307
DrdI GACnn_nn'nnGTC
Cuts at: 2013 2871 2013
Size: 858 3449
EaeI y'GGCC_r
Cuts at: 1169 1265 1892 2066 2457 2484 3829 1169
Size: 96 627 174 391 27 1345 1647
Fragments arranged by size:
1647 1345 627 391 174 96 27
EagI C'GGCC_G
Cuts at: 1892 1892
Size: 4307
EarI CTCTTCn'nnn_
Cuts at: 1113 2330 2540 1113
Size: 1217 210 2880
Fragments arranged by size:
2880 1217 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 370 994 2515 2825 2971 4117 370
Size: 624 1521 310 146 1146 560
Fragments arranged by size:
1521 1146 624 560 310 146
EcoICRI GAG'CTC
Cuts at: 1319 1319
Size: 4307
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 724 757 760 808 1261 1297 2132 2564
Size: 360 33 3 48 453 36 835 432
Cuts at: 2564 3311 3579 364
Size: 747 268 1092
Fragments arranged by size:
1092 835 747 453 432 360 268 48
36 33 3
EcoNI CCTnn'n_nnAGG
Cuts at: 450 1015 450
Size: 565 3742
EcoO109I rG'GnC_Cy
Cuts at: 409 558 636 725 1020 1040 1188 1220 1292
Size: 149 78 89 295 20 148 32 72
Cuts at: 1292 1446 409
Size: 154 3270
Fragments arranged by size:
3270 295 154 149 148 89 78 72
32 20
EcoRI G'AATT_C
Cuts at: 1311 1311
Size: 4307
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 219 2326 219
Size: 2107 2200
FspI TGC'GCA
Cuts at: 2088 2088
Size: 4307
HaeII r_GCGC'y
Cuts at: 136 1088 1741 1989 3011 3990 136
Size: 952 653 248 1022 979 453
Fragments arranged by size:
1022 979 952 653 453 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 444 476 524 556 2149 2179 2181 2211 444
Size: 32 48 32 1593 30 2 30 2540
Fragments arranged by size:
2540 1593 48 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1346 1346
Size: 4307
KasI G'GCGC_C
Cuts at: 1737 1985 1737
Size: 248 4059
KpnI G_GTAC'C
Cuts at: 129 1327 129
Size: 1198 3109
MluI A'CGCG_T
Cuts at: 3740 3740
Size: 4307
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2978 3162 2978
Size: 184 4123
MscI TGG'CCA
Cuts at: 1267 2068 1267
Size: 801 3506
MslI CAynn'nnrTG
Cuts at: 607 2296 2423 607
Size: 1689 127 2491
Fragments arranged by size:
2491 1689 127
MspA1I CmG'CkG
Cuts at: 654 799 831 1073 1418 1732 2092 3105 3350
Size: 145 32 242 345 314 360 1013 245
Cuts at: 3350 654
Size: 1611
Fragments arranged by size:
1611 1013 360 345 314 245 242 145
32
NaeI GCC'GGC
Cuts at: 2488 2488
Size: 4307
NarI GG'CG_CC
Cuts at: 1738 1986 1738
Size: 248 4059
NgoMIV G'CCGG_C
Cuts at: 2486 2486
Size: 4307
NspI r_CATG'y
Cuts at: 1360 1860 2767 1360
Size: 500 907 2900
Fragments arranged by size:
2900 907 500
PasI CC'CwG_GG
Cuts at: 141 405 1215 141
Size: 264 810 3233
Fragments arranged by size:
3233 810 264
PciI A'CATG_T
Cuts at: 2763 2763
Size: 4307
PflMI CCAn_nnn'nTGG
Cuts at: 728 831 728
Size: 103 4204
PfoI T'CCnGG_A
Cuts at: 1094 1094
Size: 4307
PmeI GTTT'AAAC
Cuts at: 1306 1306
Size: 4307
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3446 3478 3446
Size: 32 4275
PpuMI rG'GwC_Cy
Cuts at: 409 558 409
Size: 149 4158
PspOMI G'GGCC_C
Cuts at: 802 1187 802
Size: 385 3922
PstI C_TGCA'G
Cuts at: 1354 2039 1354
Size: 685 3622
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4307
PvuII CAG'CTG
Cuts at: 799 831 1732 2092 799
Size: 32 901 360 3014
Fragments arranged by size:
3014 901 360 32
RsrII CG'GwC_CG
Cuts at: 2502 2502
Size: 4307
SacI G_AGCT'C
Cuts at: 1321 1321
Size: 4307
SalI G'TCGA_C
Cuts at: 1344 1344
Size: 4307
SanDI GG'GwC_CC
Cuts at: 558 558
Size: 4307
SapI GCTCTTCn'nnn_
Cuts at: 2330 2540 2330
Size: 210 4097
SbfI CC_TGCA'GG
Cuts at: 1354 1354
Size: 4307
ScaI AGT'ACT
Cuts at: 3824 3824
Size: 4307
SfcI C'TryA_G
Cuts at: 33 1000 1106 1350 2035 3028 3219 33
Size: 967 106 244 685 993 191 1121
Fragments arranged by size:
1121 993 967 685 244 191 106
SfoI GGC'GCC
Cuts at: 1739 1987 1739
Size: 248 4059
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4307
SmaI CCC'GGG
Cuts at: 890 1329 890
Size: 439 3868
SmlI C'TyrA_G
Cuts at: 643 679 941 1462 2377 2869 3131 643
Size: 36 262 521 915 492 262 1819
Fragments arranged by size:
1819 915 521 492 262 262 36
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4307
SphI G_CATG'C
Cuts at: 1360 1860 1360
Size: 500 3807
StyI C'CwwG_G
Cuts at: 632 1441 1795 632
Size: 809 354 3144
Fragments arranged by size:
3144 809 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 133 1093 1890 2023 2482 2691 133
Size: 960 797 133 459 209 1749
Fragments arranged by size:
1749 960 797 459 209 133
TatI w'GTAC_w
Cuts at: 3822 3822
Size: 4307
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1118 1150 3824 3856 1118
Size: 32 2674 32 1569
Fragments arranged by size:
2674 1569 32 32
Tth111I GACn'n_nGTC
Cuts at: 2104 2104
Size: 4307
XbaI T'CTAG_A
Cuts at: 1338 1338
Size: 4307
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1802 1802
Size: 4307
XmaI C'CCGG_G
Cuts at: 888 1327 888
Size: 439 3868
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BseRI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI