Restriction map for
pF1KB5182
(Circular) (Six-base) MAPSORT of: pF1KB5182.seq Check: 3291 from: 1 to: 4628
pF1KB5182 4628 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 1666 1666
Size: 4628
Acc65I G'GTAC_C
Cuts at: 1644 1644
Size: 4628
AclI AA'CG_TT
Cuts at: 4468 4468
Size: 4628
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1559 1621 2453 2885 3238 3900 1559
Size: 62 832 432 353 662 2287
Fragments arranged by size:
2287 832 662 432 353 62
AflIII A'CryG_T
Cuts at: 287 1166 1980 3780 4061 287
Size: 879 814 1800 281 854
Fragments arranged by size:
1800 879 854 814 281
AleI CACnn'nnGTG
Cuts at: 1159 1159
Size: 4628
AlwNI CAG_nnn'CTG
Cuts at: 1197 1933 3371 1197
Size: 736 1438 2454
Fragments arranged by size:
2454 1438 736
ApaI G_GGCC'C
Cuts at: 339 486 1275 339
Size: 147 789 3692
Fragments arranged by size:
3692 789 147
ApaLI G'TGCA_C
Cuts at: 3466 3466
Size: 4628
ApoI r'AATT_y
Cuts at: 1057 1632 3981 4237 1057
Size: 575 2349 256 1448
Fragments arranged by size:
2349 1448 575 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4628
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
AvaI C'yCGr_G
Cuts at: 270 627 1563 1648 270
Size: 357 936 85 3250
Fragments arranged by size:
3250 936 357 85
BamHI G'GATC_C
Cuts at: 1653 1653
Size: 4628
BanI G'GyrC_C
Cuts at: 682 1644 2058 2306 2341 682
Size: 962 414 248 35 2969
Fragments arranged by size:
2969 962 414 248 35
BanII G_rGCy'C
Cuts at: 339 486 1275 1642 2672 339
Size: 147 789 367 1030 2295
Fragments arranged by size:
2295 1030 789 367 147
BbeI G_GCGC'C
Cuts at: 2062 2310 2062
Size: 248 4380
BbsI GAAGACnn'nnnn_
Cuts at: 904 925 904
Size: 21 4607
BciVI GTATCCnnnnn_n'
Cuts at: 434 616 2526 3582 4628 434
Size: 182 1910 1056 1046 434
Fragments arranged by size:
1910 1056 1046 434 182
BclI T'GATC_A
Cuts at: 857 2148 857
Size: 1291 3337
BglI GCCn_nnn'nGGC
Cuts at: 343 343
Size: 4628
BglII A'GATC_T
Cuts at: 864 1604 2143 4180 864
Size: 740 539 2037 1312
Fragments arranged by size:
2037 1312 740 539
BlpI GC'TnA_GC
Cuts at: 1740 1740
Size: 4628
Bme1580I G_kGCm'C
Cuts at: 339 365 486 659 1106 1275 2253 2346 3470
Size: 26 121 173 447 169 978 93 1124
Cuts at: 3470 339
Size: 1497
Fragments arranged by size:
1497 1124 978 447 173 169 121 93
26
BmrI ACTGGGnnnn_n'
Cuts at: 660 919 1840 1921 2256 4400 660
Size: 259 921 81 335 2144 888
Fragments arranged by size:
2144 921 888 335 259 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2995 2995
Size: 4628
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 435 560 1381 435
Size: 125 821 3682
Fragments arranged by size:
3682 821 125
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1378 1804 2683 3397 3695 1378
Size: 426 879 714 298 2311
Fragments arranged by size:
2311 879 714 426 298
BsaI GGTCTCn'nnnn_
Cuts at: 1489 4332 1489
Size: 2843 1785
BsaAI yAC'GTr
Cuts at: 1981 1981
Size: 4628
BsaBI GATnn'nnATC
Cuts at: 6 997 1474 2142 4179 6
Size: 991 477 668 2037 455
Fragments arranged by size:
2037 991 668 477 455
BsaHI Gr'CG_yC
Cuts at: 2059 2307 3009 4505 2059
Size: 248 702 1496 2182
Fragments arranged by size:
2182 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1460 1586 1817 2338 3016 3427 3574 3848 1460
Size: 126 231 521 678 411 147 274 2240
Fragments arranged by size:
2240 678 521 411 274 231 147 126
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 433 463 681 711 433
Size: 30 218 30 4350
Fragments arranged by size:
4350 218 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 185 320 1108 1378 185
Size: 135 788 270 3435
Fragments arranged by size:
3435 788 270 135
BseYI C'CCAG_C
Cuts at: 202 395 1404 2053 2811 3476 3884 202
Size: 193 1009 649 758 665 408 946
Fragments arranged by size:
1009 946 758 665 649 408 193
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 140
Size: 4628
BsiEI CG_ry'CG
Cuts at: 104 1208 2170 2216 3446 104
Size: 1104 962 46 1230 1286
Fragments arranged by size:
1286 1230 1104 962 46
BsiHKAI G_wGCw'C
Cuts at: 1025 1261 1642 2420 2610 3470 1025
Size: 236 381 778 190 860 2183
Fragments arranged by size:
2183 860 778 381 236 190
Bsp1286I G_dGCh'C
Cuts at: 339 365 486 659 1025 1106 1261 1275 1642
Size: 26 121 173 366 81 155 14 367
Cuts at: 1642 2253 2346 2420 2610 2672 3470 339
Size: 611 93 74 190 62 798 1497
Fragments arranged by size:
1497 798 611 367 366 190 173 155
121 93 81 74 62 26 14
BspHI T'CATG_A
Cuts at: 3060 3060
Size: 4628
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1678 1892 2194 2575 1678
Size: 214 302 381 3731
Fragments arranged by size:
3731 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2920 2974 4458 4623 50
Size: 2870 54 1484 165 55
Fragments arranged by size:
2870 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1023 1623 2540 1023
Size: 600 917 3111
Fragments arranged by size:
3111 917 600
BsrFI r'CCGG_y
Cuts at: 2626 2807 2626
Size: 181 4447
BssSI C'ACGA_G
Cuts at: 2899 3607 2899
Size: 708 3920
BstAPI GCAn_nnn'nTGC
Cuts at: 606 2005 606
Size: 1399 3229
BstBI TT'CG_AA
Cuts at: 1086 2989 1086
Size: 1903 2725
BstXI CCAn_nnnn'nTGG
Cuts at: 222 222
Size: 4628
BstYI r'GATC_y
Cuts at: 1 818 864 998 1604 1653 2120 2143 2478
Size: 817 46 134 606 49 467 23 335
Cuts at: 2478 2724 3030 3042 3128 4180 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 817 606 467 449 335 306 246
134 86 49 46 23 12
BtgI C'CryG_G
Cuts at: 1379 1379
Size: 4628
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2578 2759 2922 2578
Size: 181 163 4284
Fragments arranged by size:
4284 181 163
BtsI GCAGTG_nn'
Cuts at: 1871 1871
Size: 4628
DraI TTT'AAA
Cuts at: 1627 1627
Size: 4628
DraIII CAC_nnn'GTG
Cuts at: 163 163
Size: 4628
DrdI GACnn_nn'nnGTC
Cuts at: 2334 3678 2334
Size: 1344 3284
EaeI y'GGCC_r
Cuts at: 233 281 1205 1376 2213 2387 2778 2805 4150
Size: 48 924 171 837 174 391 27 1345
Cuts at: 4150 233
Size: 711
Fragments arranged by size:
1345 924 837 711 391 174 171 48
27
EagI C'GGCC_G
Cuts at: 1205 2213 1205
Size: 1008 3620
EarI CTCTTCn'nnn_
Cuts at: 1070 1324 2651 2861 1070
Size: 254 1327 210 2837
Fragments arranged by size:
2837 1327 254 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 188 458 2836 3578 3724 4438 188
Size: 270 2378 742 146 714 378
Fragments arranged by size:
2378 742 714 378 270 146
EcoICRI GAG'CTC
Cuts at: 1640 1640
Size: 4628
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 435 560 1381 1559 1621 2453 2885 3238 3900
Size: 125 821 178 62 832 432 353 662
Cuts at: 3900 435
Size: 1163
Fragments arranged by size:
1163 832 821 662 432 353 178 125
62
EcoNI CCTnn'n_nnAGG
Cuts at: 736 1075 1363 736
Size: 339 288 4001
Fragments arranged by size:
4001 339 288
EcoO109I rG'GnC_Cy
Cuts at: 335 336 482 483 533 729 1356 1767 335
Size: 1 146 1 50 196 627 411 3196
Fragments arranged by size:
3196 627 411 196 146 50 1 1
EcoRI G'AATT_C
Cuts at: 1057 1632 1057
Size: 575 4053
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 921 2647 921
Size: 1726 2902
FspI TGC'GCA
Cuts at: 2409 2409
Size: 4628
HaeII r_GCGC'y
Cuts at: 2062 2310 3540 4311 2062
Size: 248 1230 771 2379
Fragments arranged by size:
2379 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 253 285 671 703 849 856 881 888 1501
Size: 32 386 32 146 7 25 7 613
Cuts at: 1501 1533 2470 2500 2502 2532 253
Size: 32 937 30 2 30 2349
Fragments arranged by size:
2349 937 613 386 146 32 32 32
30 30 25 7 7 2
HincII GTy'rAC
Cuts at: 1667 1667
Size: 4628
KasI G'GCGC_C
Cuts at: 2058 2306 2058
Size: 248 4380
KpnI G_GTAC'C
Cuts at: 1648 1648
Size: 4628
MluI A'CGCG_T
Cuts at: 4061 4061
Size: 4628
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 824 3387 3571 824
Size: 2563 184 1881
Fragments arranged by size:
2563 1881 184
MscI TGG'CCA
Cuts at: 235 2389 235
Size: 2154 2474
MslI CAynn'nnrTG
Cuts at: 1159 1480 2617 2744 1159
Size: 321 1137 127 3043
Fragments arranged by size:
3043 1137 321 127
MspA1I CmG'CkG
Cuts at: 154 652 810 1113 1739 2053 2413 3197 3442
Size: 498 158 303 626 314 360 784 245
Cuts at: 3442 154
Size: 1340
Fragments arranged by size:
1340 784 626 498 360 314 303 245
158
NaeI GCC'GGC
Cuts at: 2809 2809
Size: 4628
NarI GG'CG_CC
Cuts at: 2059 2307 2059
Size: 248 4380
NgoMIV G'CCGG_C
Cuts at: 2807 2807
Size: 4628
NspI r_CATG'y
Cuts at: 210 291 318 1170 1374 1681 2181 3784 210
Size: 81 27 852 204 307 500 1603 1054
Fragments arranged by size:
1603 1054 852 500 307 204 81 27
PasI CC'CwG_GG
Cuts at: 173 331 340 478 529 541 589 173
Size: 158 9 138 51 12 48 4212
Fragments arranged by size:
4212 158 138 51 48 12 9
PciI A'CATG_T
Cuts at: 287 1166 3780 287
Size: 879 2614 1135
Fragments arranged by size:
2614 1135 879
PflMI CCAn_nnn'nTGG
Cuts at: 166 589 166
Size: 423 4205
PfoI T'CCnGG_A
Cuts at: 451 1261 1505 451
Size: 810 244 3574
Fragments arranged by size:
3574 810 244
PmeI GTTT'AAAC
Cuts at: 1627 1627
Size: 4628
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1225 1257 3074 3106 1225
Size: 32 1817 32 2747
Fragments arranged by size:
2747 1817 32 32
PpuMI rG'GwC_Cy
Cuts at: 729 1356 729
Size: 627 4001
PshAI GACnn'nnGTC
Cuts at: 925 925
Size: 4628
PspOMI G'GGCC_C
Cuts at: 335 482 1271 335
Size: 147 789 3692
Fragments arranged by size:
3692 789 147
PstI C_TGCA'G
Cuts at: 1675 2360 1675
Size: 685 3943
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4628
PvuII CAG'CTG
Cuts at: 154 2053 2413 154
Size: 1899 360 2369
Fragments arranged by size:
2369 1899 360
RsrII CG'GwC_CG
Cuts at: 2823 2823
Size: 4628
SacI G_AGCT'C
Cuts at: 1642 1642
Size: 4628
SalI G'TCGA_C
Cuts at: 1665 1665
Size: 4628
SapI GCTCTTCn'nnn_
Cuts at: 1324 2651 2861 1324
Size: 1327 210 3091
Fragments arranged by size:
3091 1327 210
SbfI CC_TGCA'GG
Cuts at: 1675 1675
Size: 4628
ScaI AGT'ACT
Cuts at: 4145 4145
Size: 4628
SexAI A'CCwGG_T
Cuts at: 159 1277 159
Size: 1118 3510
SfcI C'TryA_G
Cuts at: 33 1671 2356 3324 3515 33
Size: 1638 685 968 191 1146
Fragments arranged by size:
1638 1146 968 685 191
SfoI GGC'GCC
Cuts at: 2060 2308 2060
Size: 248 4380
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
SmaI CCC'GGG
Cuts at: 629 1650 629
Size: 1021 3607
SmlI C'TyrA_G
Cuts at: 1393 1783 2698 3412 3674 1393
Size: 390 915 714 262 2347
Fragments arranged by size:
2347 915 714 390 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4628
SphI G_CATG'C
Cuts at: 210 1374 1681 2181 210
Size: 1164 307 500 2657
Fragments arranged by size:
2657 1164 500 307
StyI C'CwwG_G
Cuts at: 1762 2116 1762
Size: 354 4274
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1513 2211 2344 2803 3012 1513
Size: 698 133 459 209 3129
Fragments arranged by size:
3129 698 459 209 133
TatI w'GTAC_w
Cuts at: 4143 4143
Size: 4628
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 681 713 4145 4177 681
Size: 32 3432 32 1132
Fragments arranged by size:
3432 1132 32 32
Tth111I GACn'n_nGTC
Cuts at: 2425 2425
Size: 4628
XbaI T'CTAG_A
Cuts at: 1659 1659
Size: 4628
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2123 2123
Size: 4628
XmaI C'CCGG_G
Cuts at: 627 1648 627
Size: 1021 3607
XmnI GAAnn'nnTTC
Cuts at: 751 751
Size: 4628
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PmlI PsiI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI