Restriction map for
pF1KB5162
(Circular) (Six-base) MAPSORT of: pF1KB5162.seq Check: 2032 from: 1 to: 3764
pF1KB5162 3764 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 802 802
Size: 3764
Acc65I G'GTAC_C
Cuts at: 780 780
Size: 3764
AclI AA'CG_TT
Cuts at: 3604 3604
Size: 3764
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1589 2021 2374 3036 1589
Size: 432 353 662 2317
Fragments arranged by size:
2317 662 432 353
AfeI AGC'GCT
Cuts at: 378 378
Size: 3764
AflIII A'CryG_T
Cuts at: 1116 2916 3197 1116
Size: 1800 281 1683
Fragments arranged by size:
1800 1683 281
AleI CACnn'nnGTG
Cuts at: 282 742 282
Size: 460 3304
AlwNI CAG_nnn'CTG
Cuts at: 1069 2507 1069
Size: 1438 2326
ApaLI G'TGCA_C
Cuts at: 2602 2602
Size: 3764
ApoI r'AATT_y
Cuts at: 768 3117 3373 768
Size: 2349 256 1159
Fragments arranged by size:
2349 1159 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3764
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
AvaI C'yCGr_G
Cuts at: 784 784
Size: 3764
BamHI G'GATC_C
Cuts at: 789 789
Size: 3764
BanI G'GyrC_C
Cuts at: 567 780 1194 1442 1477 567
Size: 213 414 248 35 2854
Fragments arranged by size:
2854 414 248 213 35
BanII G_rGCy'C
Cuts at: 778 1808 778
Size: 1030 2734
BbeI G_GCGC'C
Cuts at: 1198 1446 1198
Size: 248 3516
BciVI GTATCCnnnnn_n'
Cuts at: 1662 2718 3764 1662
Size: 1056 1046 1662
Fragments arranged by size:
1662 1056 1046
BclI T'GATC_A
Cuts at: 204 1284 204
Size: 1080 2684
BglII A'GATC_T
Cuts at: 514 1279 3316 514
Size: 765 2037 962
Fragments arranged by size:
2037 962 765
BlpI GC'TnA_GC
Cuts at: 157 328 876 157
Size: 171 548 3045
Fragments arranged by size:
3045 548 171
Bme1580I G_kGCm'C
Cuts at: 1389 1482 2606 1389
Size: 93 1124 2547
Fragments arranged by size:
2547 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 976 1057 1392 3536 976
Size: 81 335 2144 1204
Fragments arranged by size:
2144 1204 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 3764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 513 513
Size: 3764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 940 1819 2533 2831 166
Size: 774 879 714 298 1099
Fragments arranged by size:
1099 879 774 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3468 3468
Size: 3764
BsaAI yAC'GTr
Cuts at: 1117 1117
Size: 3764
BsaBI GATnn'nnATC
Cuts at: 6 519 1278 3315 6
Size: 513 759 2037 455
Fragments arranged by size:
2037 759 513 455
BsaHI Gr'CG_yC
Cuts at: 1195 1443 2145 3641 1195
Size: 248 702 1496 1318
Fragments arranged by size:
1496 1318 702 248
BsaWI w'CCGG_w
Cuts at: 749 953 1474 2152 2563 2710 2984 749
Size: 204 521 678 411 147 274 1529
Fragments arranged by size:
1529 678 521 411 274 204 147
BseYI C'CCAG_C
Cuts at: 630 1189 1947 2612 3020 630
Size: 559 758 665 408 1374
Fragments arranged by size:
1374 758 665 559 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 442
Size: 3764
BsiEI CG_ry'CG
Cuts at: 104 1306 1352 2582 104
Size: 1202 46 1230 1286
Fragments arranged by size:
1286 1230 1202 46
BsiHKAI G_wGCw'C
Cuts at: 440 778 1556 1746 2606 440
Size: 338 778 190 860 1598
Fragments arranged by size:
1598 860 778 338 190
Bsp1286I G_dGCh'C
Cuts at: 440 778 1389 1482 1556 1746 1808 2606 440
Size: 338 611 93 74 190 62 798 1598
Fragments arranged by size:
1598 798 611 338 190 93 74 62
BspHI T'CATG_A
Cuts at: 2196 2196
Size: 3764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 814 1028 1330 1711 814
Size: 214 302 381 2867
Fragments arranged by size:
2867 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2056 2110 3594 3759 50
Size: 2006 54 1484 165 55
Fragments arranged by size:
2006 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1676 1676
Size: 3764
BsrFI r'CCGG_y
Cuts at: 712 1762 1943 712
Size: 1050 181 2533
Fragments arranged by size:
2533 1050 181
BssSI C'ACGA_G
Cuts at: 2035 2743 2035
Size: 708 3056
BstAPI GCAn_nnn'nTGC
Cuts at: 1141 1141
Size: 3764
BstBI TT'CG_AA
Cuts at: 2125 2125
Size: 3764
BstXI CCAn_nnnn'nTGG
Cuts at: 284 622 284
Size: 338 3426
BstYI r'GATC_y
Cuts at: 1 514 789 1256 1279 1614 1860 2166 2178
Size: 513 275 467 23 335 246 306 12
Cuts at: 2178 2264 3316 1
Size: 86 1052 449
Fragments arranged by size:
1052 513 467 449 335 306 275 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 283 702 107
Size: 176 419 3169
Fragments arranged by size:
3169 419 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 102 1714 1895 2058 102
Size: 1612 181 163 1808
Fragments arranged by size:
1808 1612 181 163
BtsI GCAGTG_nn'
Cuts at: 1007 1007
Size: 3764
DraI TTT'AAA
Cuts at: 763 763
Size: 3764
DrdI GACnn_nn'nnGTC
Cuts at: 1470 2814 1470
Size: 1344 2420
EaeI y'GGCC_r
Cuts at: 1349 1523 1914 1941 3286 1349
Size: 174 391 27 1345 1827
Fragments arranged by size:
1827 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1349 1349
Size: 3764
EarI CTCTTCn'nnn_
Cuts at: 1787 1997 1787
Size: 210 3554
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 628 1972 2714 2860 3574 628
Size: 1344 742 146 714 818
Fragments arranged by size:
1344 818 742 714 146
EcoICRI GAG'CTC
Cuts at: 776 776
Size: 3764
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 513 1589 2021 2374 3036 513
Size: 1076 432 353 662 1241
Fragments arranged by size:
1241 1076 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 350 594 903 350
Size: 244 309 3211
Fragments arranged by size:
3211 309 244
EcoRI G'AATT_C
Cuts at: 768 768
Size: 3764
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 343 1783 343
Size: 1440 2324
FspI TGC'GCA
Cuts at: 1545 1545
Size: 3764
HaeII r_GCGC'y
Cuts at: 380 1198 1446 2676 3447 380
Size: 818 248 1230 771 697
Fragments arranged by size:
1230 818 771 697 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 638 670 1606 1636 1638 1668 638
Size: 32 936 30 2 30 2734
Fragments arranged by size:
2734 936 32 30 30 2
HincII GTy'rAC
Cuts at: 803 803
Size: 3764
KasI G'GCGC_C
Cuts at: 1194 1442 1194
Size: 248 3516
KpnI G_GTAC'C
Cuts at: 784 784
Size: 3764
MluI A'CGCG_T
Cuts at: 3197 3197
Size: 3764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2523 2707 2523
Size: 184 3580
MscI TGG'CCA
Cuts at: 1525 1525
Size: 3764
MslI CAynn'nnrTG
Cuts at: 268 282 742 1753 1880 268
Size: 14 460 1011 127 2152
Fragments arranged by size:
2152 1011 460 127 14
MspA1I CmG'CkG
Cuts at: 339 875 1189 1549 2333 2578 339
Size: 536 314 360 784 245 1525
Fragments arranged by size:
1525 784 536 360 314 245
NaeI GCC'GGC
Cuts at: 1945 1945
Size: 3764
NarI GG'CG_CC
Cuts at: 1195 1443 1195
Size: 248 3516
NcoI C'CATG_G
Cuts at: 107 107
Size: 3764
NgoMIV G'CCGG_C
Cuts at: 1943 1943
Size: 3764
NspI r_CATG'y
Cuts at: 720 817 1317 2920 720
Size: 97 500 1603 1564
Fragments arranged by size:
1603 1564 500 97
PciI A'CATG_T
Cuts at: 2916 2916
Size: 3764
PflMI CCAn_nnn'nTGG
Cuts at: 663 686 663
Size: 23 3741
PmeI GTTT'AAAC
Cuts at: 763 763
Size: 3764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2210 2242 2210
Size: 32 3732
PshAI GACnn'nnGTC
Cuts at: 586 586
Size: 3764
PstI C_TGCA'G
Cuts at: 147 811 1496 147
Size: 664 685 2415
Fragments arranged by size:
2415 685 664
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3764
PvuII CAG'CTG
Cuts at: 1189 1549 1189
Size: 360 3404
RsrII CG'GwC_CG
Cuts at: 1959 1959
Size: 3764
SacI G_AGCT'C
Cuts at: 778 778
Size: 3764
SalI G'TCGA_C
Cuts at: 801 801
Size: 3764
SapI GCTCTTCn'nnn_
Cuts at: 1787 1997 1787
Size: 210 3554
SbfI CC_TGCA'GG
Cuts at: 811 811
Size: 3764
ScaI AGT'ACT
Cuts at: 460 3281 460
Size: 2821 943
SfcI C'TryA_G
Cuts at: 33 143 807 1492 2460 2651 33
Size: 110 664 685 968 191 1146
Fragments arranged by size:
1146 968 685 664 191 110
SfoI GGC'GCC
Cuts at: 1196 1444 1196
Size: 248 3516
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
SmaI CCC'GGG
Cuts at: 786 786
Size: 3764
SmlI C'TyrA_G
Cuts at: 181 919 1834 2548 2810 181
Size: 738 915 714 262 1135
Fragments arranged by size:
1135 915 738 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3764
SphI G_CATG'C
Cuts at: 817 1317 817
Size: 500 3264
StyI C'CwwG_G
Cuts at: 107 311 898 1252 107
Size: 204 587 354 2619
Fragments arranged by size:
2619 587 354 204
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 459 1347 1480 1939 2148 459
Size: 888 133 459 209 2075
Fragments arranged by size:
2075 888 459 209 133
TatI w'GTAC_w
Cuts at: 458 3279 458
Size: 2821 943
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 404 436 3281 3313 404
Size: 32 2845 32 855
Fragments arranged by size:
2845 855 32 32
Tth111I GACn'n_nGTC
Cuts at: 1561 1561
Size: 3764
XbaI T'CTAG_A
Cuts at: 795 795
Size: 3764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1259 1259
Size: 3764
XmaI C'CCGG_G
Cuts at: 784 784
Size: 3764
XmnI GAAnn'nnTTC
Cuts at: 460 460
Size: 3764
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI
PshAI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI
BspEI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI