Restriction map for
pF1KB5154
(Circular) (Six-base) MAPSORT of: pF1KB5154.seq Check: 3566 from: 1 to: 4997
pF1KB5154 4997 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AatII G_ACGT'C
Cuts at: 1562 1937 1562
Size: 375 4622
AccI GT'mk_AC
Cuts at: 516 940 2035 516
Size: 424 1095 3478
Fragments arranged by size:
3478 1095 424
Acc65I G'GTAC_C
Cuts at: 2013 2013
Size: 4997
AclI AA'CG_TT
Cuts at: 4837 4837
Size: 4997
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 308 1607 1726 2822 3254 3607 4269 308
Size: 1299 119 1096 432 353 662 1036
Fragments arranged by size:
1299 1096 1036 662 432 353 119
AflIII A'CryG_T
Cuts at: 2349 4149 4430 2349
Size: 1800 281 2916
Fragments arranged by size:
2916 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 130 1232 2302 3740 130
Size: 1102 1070 1438 1387
Fragments arranged by size:
1438 1387 1102 1070
ApaLI G'TGCA_C
Cuts at: 3835 3835
Size: 4997
ApoI r'AATT_y
Cuts at: 865 951 1471 1645 2001 4350 4606 865
Size: 86 520 174 356 2349 256 1256
Fragments arranged by size:
2349 1256 520 356 256 174 86
AseI AT'TA_AT
Cuts at: 20 1577 20
Size: 1557 3440
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4997
AvaI C'yCGr_G
Cuts at: 1171 2017 1171
Size: 846 4151
AvrII C'CTAG_G
Cuts at: 1437 1437
Size: 4997
BamHI G'GATC_C
Cuts at: 2022 2022
Size: 4997
BanI G'GyrC_C
Cuts at: 145 1363 1906 2013 2427 2675 2710 145
Size: 1218 543 107 414 248 35 2432
Fragments arranged by size:
2432 1218 543 414 248 107 35
BanII G_rGCy'C
Cuts at: 272 409 1275 2011 3041 272
Size: 137 866 736 1030 2228
Fragments arranged by size:
2228 1030 866 736 137
BbeI G_GCGC'C
Cuts at: 1910 2431 2679 1910
Size: 521 248 4228
Fragments arranged by size:
4228 521 248
BbsI GAAGACnn'nnnn_
Cuts at: 676 1595 676
Size: 919 4078
BciVI GTATCCnnnnn_n'
Cuts at: 369 387 1868 2895 3951 4997 369
Size: 18 1481 1027 1056 1046 369
Fragments arranged by size:
1481 1056 1046 1027 369 18
BclI T'GATC_A
Cuts at: 2517 2517
Size: 4997
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4997
BglII A'GATC_T
Cuts at: 1286 2512 4549 1286
Size: 1226 2037 1734
Fragments arranged by size:
2037 1734 1226
BlpI GC'TnA_GC
Cuts at: 1073 1145 2109 1073
Size: 72 964 3961
Fragments arranged by size:
3961 964 72
Bme1580I G_kGCm'C
Cuts at: 2622 2715 3839 2622
Size: 93 1124 3780
Fragments arranged by size:
3780 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 266 729 1272 1429 2209 2290 2625 4769 266
Size: 463 543 157 780 81 335 2144 494
Fragments arranged by size:
2144 780 543 494 463 335 157 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 264 1361 3364 264
Size: 1097 2003 1897
Fragments arranged by size:
2003 1897 1097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 1235 257
Size: 978 4019
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2173 3052 3766 4064 2173
Size: 879 714 298 3106
Fragments arranged by size:
3106 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 738 1211 4701 738
Size: 473 3490 1034
Fragments arranged by size:
3490 1034 473
BsaAI yAC'GTr
Cuts at: 1947 2350 1947
Size: 403 4594
BsaBI GATnn'nnATC
Cuts at: 6 662 1127 2511 4548 6
Size: 656 465 1384 2037 455
Fragments arranged by size:
2037 1384 656 465 455
BsaHI Gr'CG_yC
Cuts at: 1559 1907 1934 2428 2676 3378 4874 1559
Size: 348 27 494 248 702 1496 1682
Fragments arranged by size:
1682 1496 702 494 348 248 27
BsaWI w'CCGG_w
Cuts at: 584 2186 2707 3385 3796 3943 4217 584
Size: 1602 521 678 411 147 274 1364
Fragments arranged by size:
1602 1364 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1959 1989 1959
Size: 30 4967
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 221 1010 221
Size: 789 4208
BseYI C'CCAG_C
Cuts at: 370 1193 1430 1797 1901 2422 3180 3845 4253
Size: 823 237 367 104 521 758 665 408
Cuts at: 4253 370
Size: 1114
Fragments arranged by size:
1114 823 758 665 521 408 367 237
104
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1806 1806
Size: 4997
BsiEI CG_ry'CG
Cuts at: 104 170 2539 2585 3815 104
Size: 66 2369 46 1230 1286
Fragments arranged by size:
2369 1286 1230 66 46
BsiHKAI G_wGCw'C
Cuts at: 1275 1922 2011 2789 2979 3839 1275
Size: 647 89 778 190 860 2433
Fragments arranged by size:
2433 860 778 647 190 89
BsmBI CGTCTCn'nnnn_
Cuts at: 1550 1550
Size: 4997
Bsp1286I G_dGCh'C
Cuts at: 272 409 1275 1922 2011 2622 2715 2789 2979
Size: 137 866 647 89 611 93 74 190
Cuts at: 2979 3041 3839 272
Size: 62 798 1430
Fragments arranged by size:
1430 866 798 647 611 190 137 93
89 74 62
BspEI T'CCGG_A
Cuts at: 584 584
Size: 4997
BspHI T'CATG_A
Cuts at: 524 3429 524
Size: 2905 2092
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2047 2261 2563 2944 2047
Size: 214 302 381 4100
Fragments arranged by size:
4100 381 302 214
BsrBI CCG'CTC
Cuts at: 50 166 229 3289 3343 4827 4992 50
Size: 116 63 3060 54 1484 165 55
Fragments arranged by size:
3060 1484 165 116 63 55 54
BsrDI GCAATG_nn'
Cuts at: 460 543 1609 2909 460
Size: 83 1066 1300 2548
Fragments arranged by size:
2548 1300 1066 83
BsrFI r'CCGG_y
Cuts at: 207 1298 2995 3176 207
Size: 1091 1697 181 2028
Fragments arranged by size:
2028 1697 1091 181
BsrGI T'GTAC_A
Cuts at: 466 1811 466
Size: 1345 3652
BssSI C'ACGA_G
Cuts at: 3268 3976 3268
Size: 708 4289
BstAPI GCAn_nnn'nTGC
Cuts at: 554 2374 554
Size: 1820 3177
BstBI TT'CG_AA
Cuts at: 3358 3358
Size: 4997
BstXI CCAn_nnnn'nTGG
Cuts at: 565 1522 565
Size: 957 4040
BstYI r'GATC_y
Cuts at: 1 245 828 843 1286 1869 2022 2489 2512
Size: 244 583 15 443 583 153 467 23
Cuts at: 2512 2847 3093 3399 3411 3497 4549 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 583 583 467 449 443 335 306
246 244 153 86 23 15 12
BtgI C'CryG_G
Cuts at: 107 410 1910 1981 107
Size: 303 1500 71 3123
Fragments arranged by size:
3123 1500 303 71
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2947 3128 3291 2947
Size: 181 163 4653
Fragments arranged by size:
4653 181 163
BtsI GCAGTG_nn'
Cuts at: 936 2240 936
Size: 1304 3693
DraI TTT'AAA
Cuts at: 956 1996 956
Size: 1040 3957
DrdI GACnn_nn'nnGTC
Cuts at: 642 1605 2703 4047 642
Size: 963 1098 1344 1592
Fragments arranged by size:
1592 1344 1098 963
EaeI y'GGCC_r
Cuts at: 132 426 626 763 2582 2756 3147 3174 4519
Size: 294 200 137 1819 174 391 27 1345
Cuts at: 4519 132
Size: 610
Fragments arranged by size:
1819 1345 610 391 294 200 174 137
27
EagI C'GGCC_G
Cuts at: 2582 2582
Size: 4997
EarI CTCTTCn'nnn_
Cuts at: 185 413 1542 1693 3020 3230 185
Size: 228 1129 151 1327 210 1952
Fragments arranged by size:
1952 1327 1129 228 210 151
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1503 3205 3947 4093 4807 1503
Size: 1702 742 146 714 1693
Fragments arranged by size:
1702 1693 742 714 146
EcoICRI GAG'CTC
Cuts at: 1273 2009 1273
Size: 736 4261
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 308 1235 1607 1726 2822 3254 3607 4269
Size: 51 927 372 119 1096 432 353 662
Cuts at: 4269 257
Size: 985
Fragments arranged by size:
1096 985 927 662 432 372 353 119
51
EcoNI CCTnn'n_nnAGG
Cuts at: 158 158
Size: 4997
EcoO109I rG'GnC_Cy
Cuts at: 127 885 1340 2136 127
Size: 758 455 796 2988
Fragments arranged by size:
2988 796 758 455
EcoRI G'AATT_C
Cuts at: 2001 2001
Size: 4997
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3016 3016
Size: 4997
FspI TGC'GCA
Cuts at: 2778 2778
Size: 4997
HaeII r_GCGC'y
Cuts at: 1080 1910 2431 2679 3909 4680 1080
Size: 830 521 248 1230 771 1397
Fragments arranged by size:
1397 1230 830 771 521 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 230 262 322 354 381 413 493 525 1959
Size: 32 60 32 27 32 80 32 1434
Cuts at: 1959 1991 2839 2869 2871 2901 230
Size: 32 848 30 2 30 2326
Fragments arranged by size:
2326 1434 848 80 60 32 32 32
32 32 30 30 27 2
HincII GTy'rAC
Cuts at: 2036 2036
Size: 4997
KasI G'GCGC_C
Cuts at: 1906 2427 2675 1906
Size: 521 248 4228
Fragments arranged by size:
4228 521 248
KpnI G_GTAC'C
Cuts at: 2017 2017
Size: 4997
MluI A'CGCG_T
Cuts at: 4430 4430
Size: 4997
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 283 1293 3756 3940 283
Size: 1010 2463 184 1340
Fragments arranged by size:
2463 1340 1010 184
MscI TGG'CCA
Cuts at: 134 628 765 2758 134
Size: 494 137 1993 2373
Fragments arranged by size:
2373 1993 494 137
MslI CAynn'nnrTG
Cuts at: 563 2986 3113 563
Size: 2423 127 2447
Fragments arranged by size:
2447 2423 127
MspA1I CmG'CkG
Cuts at: 217 983 1411 2108 2422 2782 3566 3811 217
Size: 766 428 697 314 360 784 245 1403
Fragments arranged by size:
1403 784 766 697 428 360 314 245
NaeI GCC'GGC
Cuts at: 3178 3178
Size: 4997
NarI GG'CG_CC
Cuts at: 1907 2428 2676 1907
Size: 521 248 4228
Fragments arranged by size:
4228 521 248
NcoI C'CATG_G
Cuts at: 107 1981 107
Size: 1874 3123
NgoMIV G'CCGG_C
Cuts at: 3176 3176
Size: 4997
NspI r_CATG'y
Cuts at: 2050 2550 4153 2050
Size: 500 1603 2894
Fragments arranged by size:
2894 1603 500
PciI A'CATG_T
Cuts at: 4149 4149
Size: 4997
PfoI T'CCnGG_A
Cuts at: 234 1212 234
Size: 978 4019
PmeI GTTT'AAAC
Cuts at: 1996 1996
Size: 4997
PmlI CAC'GTG
Cuts at: 1947 1947
Size: 4997
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3443 3475 3443
Size: 32 4965
PpuMI rG'GwC_Cy
Cuts at: 127 885 127
Size: 758 4239
PsiI TTA'TAA
Cuts at: 1739 1739
Size: 4997
PstI C_TGCA'G
Cuts at: 2044 2729 2044
Size: 685 4312
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4997
PvuII CAG'CTG
Cuts at: 2422 2782 2422
Size: 360 4637
RsrII CG'GwC_CG
Cuts at: 3192 3192
Size: 4997
SacI G_AGCT'C
Cuts at: 1275 2011 1275
Size: 736 4261
SalI G'TCGA_C
Cuts at: 2034 2034
Size: 4997
SapI GCTCTTCn'nnn_
Cuts at: 1693 3020 3230 1693
Size: 1327 210 3460
Fragments arranged by size:
3460 1327 210
SbfI CC_TGCA'GG
Cuts at: 2044 2044
Size: 4997
ScaI AGT'ACT
Cuts at: 4514 4514
Size: 4997
SfcI C'TryA_G
Cuts at: 33 534 1224 2040 2725 3693 3884 33
Size: 501 690 816 685 968 191 1146
Fragments arranged by size:
1146 968 816 690 685 501 191
SfoI GGC'GCC
Cuts at: 1908 2429 2677 1908
Size: 521 248 4228
Fragments arranged by size:
4228 521 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4997
SmaI CCC'GGG
Cuts at: 2019 2019
Size: 4997
SmlI C'TyrA_G
Cuts at: 2152 3067 3781 4043 2152
Size: 915 714 262 3106
Fragments arranged by size:
3106 915 714 262
SpeI A'CTAG_T
Cuts at: 67 943 67
Size: 876 4121
SphI G_CATG'C
Cuts at: 2050 2550 2050
Size: 500 4497
SspI AAT'ATT
Cuts at: 1733 1733
Size: 4997
StyI C'CwwG_G
Cuts at: 107 1437 1981 2131 2485 107
Size: 1330 544 150 354 2619
Fragments arranged by size:
2619 1330 544 354 150
SwaI ATTT'AAAT
Cuts at: 956 956
Size: 4997
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 427 839 2580 2713 3172 3381 427
Size: 412 1741 133 459 209 2043
Fragments arranged by size:
2043 1741 459 412 209 133
TatI w'GTAC_w
Cuts at: 466 1811 4512 466
Size: 1345 2701 951
Fragments arranged by size:
2701 1345 951
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4514 4546 4514
Size: 32 4965
Tth111I GACn'n_nGTC
Cuts at: 2794 2794
Size: 4997
XbaI T'CTAG_A
Cuts at: 2028 2028
Size: 4997
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1519 2492 1519
Size: 973 4024
XmaI C'CCGG_G
Cuts at: 2017 2017
Size: 4997
ZraI GAC'GTC
Cuts at: 1560 1935 1560
Size: 375 4622
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PmlI
PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AleI AlfI AloI
ApaI AscI BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsiWI BsmI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI XhoI XmnI