Restriction map for
pF1KB5152
(Circular) (Six-base) MAPSORT of: pF1KB5152.seq Check: 9327 from: 1 to: 5075
pF1KB5152 5075 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1561 1561
Size: 5075
AccI GT'mk_AC
Cuts at: 2113 2113
Size: 5075
Acc65I G'GTAC_C
Cuts at: 2091 2091
Size: 5075
AclI AA'CG_TT
Cuts at: 4915 4915
Size: 5075
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 315 1690 2900 3332 3685 4347 156
Size: 159 1375 1210 432 353 662 884
Fragments arranged by size:
1375 1210 884 662 432 353 159
AflIII A'CryG_T
Cuts at: 1628 2427 4227 4508 1628
Size: 799 1800 281 2195
Fragments arranged by size:
2195 1800 799 281
AlwNI CAG_nnn'CTG
Cuts at: 1194 1639 2380 3818 1194
Size: 445 741 1438 2451
Fragments arranged by size:
2451 1438 741 445
ApaI G_GGCC'C
Cuts at: 518 821 1686 518
Size: 303 865 3907
Fragments arranged by size:
3907 865 303
ApaLI G'TGCA_C
Cuts at: 3913 3913
Size: 5075
ApoI r'AATT_y
Cuts at: 2079 4428 4684 2079
Size: 2349 256 2470
Fragments arranged by size:
2470 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5075
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5075
AvaI C'yCGr_G
Cuts at: 511 798 1067 1217 1450 1679 1775 2095 511
Size: 287 269 150 233 229 96 320 3491
Fragments arranged by size:
3491 320 287 269 233 229 150 96
BamHI G'GATC_C
Cuts at: 2100 2100
Size: 5075
BanI G'GyrC_C
Cuts at: 129 340 424 1234 1516 1573 2091 2505 2753
Size: 211 84 810 282 57 518 414 248
Cuts at: 2753 2788 129
Size: 35 2416
Fragments arranged by size:
2416 810 518 414 282 248 211 84
57 35
BanII G_rGCy'C
Cuts at: 155 518 788 821 838 968 1066 1183 1230
Size: 363 270 33 17 130 98 117 47
Cuts at: 1230 1686 2089 3119 155
Size: 456 403 1030 2111
Fragments arranged by size:
2111 1030 456 403 363 270 130 117
98 47 33 17
BbeI G_GCGC'C
Cuts at: 133 428 2509 2757 133
Size: 295 2081 248 2451
Fragments arranged by size:
2451 2081 295 248
BbsI GAAGACnn'nnnn_
Cuts at: 637 1295 637
Size: 658 4417
BbvCI CC'TCA_GC
Cuts at: 777 990 777
Size: 213 4862
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1100 1134 1100
Size: 34 5041
BciVI GTATCCnnnnn_n'
Cuts at: 2973 4029 5075 2973
Size: 1056 1046 2973
Fragments arranged by size:
2973 1056 1046
BclI T'GATC_A
Cuts at: 2595 2595
Size: 5075
BglI GCCn_nnn'nGGC
Cuts at: 226 809 875 1061 1466 1514 1582 1681 226
Size: 583 66 186 405 48 68 99 3620
Fragments arranged by size:
3620 583 405 186 99 68 66 48
BglII A'GATC_T
Cuts at: 2590 4627 2590
Size: 2037 3038
BlpI GC'TnA_GC
Cuts at: 2187 2187
Size: 5075
Bme1580I G_kGCm'C
Cuts at: 142 343 518 821 1339 1686 2066 2700 2793
Size: 201 175 303 518 347 380 634 93
Cuts at: 2793 3917 142
Size: 1124 1300
Fragments arranged by size:
1300 1124 634 518 380 347 303 201
175 93
BmgBI CAC'GTC
Cuts at: 1631 1631
Size: 5075
BmrI ACTGGGnnnn_n'
Cuts at: 543 2287 2368 2703 4847 543
Size: 1744 81 335 2144 771
Fragments arranged by size:
2144 1744 771 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3442 3442
Size: 5075
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 810 1005 810
Size: 195 4880
Bpu10I CC'TnA_GC
Cuts at: 273 672 777 990 2038 273
Size: 399 105 213 1048 3310
Fragments arranged by size:
3310 1048 399 213 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 1125 2251 3130 3844 4142 277
Size: 848 1126 879 714 298 1210
Fragments arranged by size:
1210 1126 879 848 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1267 1994 4779 1267
Size: 727 2785 1563
Fragments arranged by size:
2785 1563 727
BsaAI yAC'GTr
Cuts at: 753 1007 1314 2428 753
Size: 254 307 1114 3400
Fragments arranged by size:
3400 1114 307 254
BsaBI GATnn'nnATC
Cuts at: 6 2589 4626 6
Size: 2583 2037 455
Fragments arranged by size:
2583 2037 455
BsaHI Gr'CG_yC
Cuts at: 130 425 1016 2506 2754 3456 4952 130
Size: 295 591 1490 248 702 1496 253
Fragments arranged by size:
1496 1490 702 591 295 253 248
BsaWI w'CCGG_w
Cuts at: 626 2264 2785 3463 3874 4021 4295 626
Size: 1638 521 678 411 147 274 1406
Fragments arranged by size:
1638 1406 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 544 574 938 968 1615 1645 544
Size: 30 364 30 647 30 3974
Fragments arranged by size:
3974 647 364 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 297 978 1621 1636 1666 1669 297
Size: 681 643 15 30 3 3703
Fragments arranged by size:
3703 681 643 30 15 3
BseYI C'CCAG_C
Cuts at: 268 319 667 724 825 1246 1410 1504 1821
Size: 51 348 57 101 421 164 94 317
Cuts at: 1821 2500 3258 3923 4331 268
Size: 679 758 665 408 1012
Fragments arranged by size:
1012 758 679 665 421 408 348 317
164 101 94 57 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 895 1825 1867 754
Size: 141 930 42 3962
Fragments arranged by size:
3962 930 141 42
BsiEI CG_ry'CG
Cuts at: 104 1586 2617 2663 3893 104
Size: 1482 1031 46 1230 1286
Fragments arranged by size:
1482 1286 1230 1031 46
BsiHKAI G_wGCw'C
Cuts at: 155 788 1319 2089 2867 3057 3917 155
Size: 633 531 770 778 190 860 1313
Fragments arranged by size:
1313 860 778 770 633 531 190
BsiWI C'GTAC_G
Cuts at: 1007 1007
Size: 5075
BsmBI CGTCTCn'nnnn_
Cuts at: 440 1023 440
Size: 583 4492
Bsp1286I G_dGCh'C
Cuts at: 142 155 343 518 788 821 838 968 1066
Size: 13 188 175 270 33 17 130 98
Cuts at: 1066 1183 1230 1319 1339 1686 2066 2089 2700
Size: 117 47 89 20 347 380 23 611
Cuts at: 2700 2793 2867 3057 3119 3917 142
Size: 93 74 190 62 798 1300
Fragments arranged by size:
1300 798 611 380 347 270 190 188
175 130 117 98 93 89 74 62
47 33 23 20 17 13
BspEI T'CCGG_A
Cuts at: 626 626
Size: 5075
BspHI T'CATG_A
Cuts at: 3507 3507
Size: 5075
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1561 2125 2339 2641 3022 1561
Size: 564 214 302 381 3614
Fragments arranged by size:
3614 564 381 302 214
BsrBI CCG'CTC
Cuts at: 50 413 420 589 1743 1782 1932 3367 3421
Size: 363 7 169 1154 39 150 1435 54
Cuts at: 3421 4905 5070 50
Size: 1484 165 55
Fragments arranged by size:
1484 1435 1154 363 169 165 150 55
54 39 7
BsrDI GCAATG_nn'
Cuts at: 2987 2987
Size: 5075
BsrFI r'CCGG_y
Cuts at: 212 263 1342 1396 3073 3254 212
Size: 51 1079 54 1677 181 2033
Fragments arranged by size:
2033 1677 1079 181 54 51
BssSI C'ACGA_G
Cuts at: 919 3346 4054 919
Size: 2427 708 1940
Fragments arranged by size:
2427 1940 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1359 1923 2452 1359
Size: 564 529 3982
Fragments arranged by size:
3982 564 529
BstBI TT'CG_AA
Cuts at: 3436 3436
Size: 5075
BstXI CCAn_nnnn'nTGG
Cuts at: 387 943 1410 387
Size: 556 467 4052
Fragments arranged by size:
4052 556 467
BstYI r'GATC_y
Cuts at: 1 2100 2567 2590 2925 3171 3477 3489 3575
Size: 2099 467 23 335 246 306 12 86
Cuts at: 3575 4627 1
Size: 1052 449
Fragments arranged by size:
2099 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1386 1386
Size: 5075
BtgI C'CryG_G
Cuts at: 1175 1266 1331 1371 1577 1175
Size: 91 65 40 206 4673
Fragments arranged by size:
4673 206 91 65 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3025 3206 3369 3025
Size: 181 163 4731
Fragments arranged by size:
4731 181 163
BtsI GCAGTG_nn'
Cuts at: 888 2318 888
Size: 1430 3645
DraI TTT'AAA
Cuts at: 2074 2074
Size: 5075
DraIII CAC_nnn'GTG
Cuts at: 1332 1332
Size: 5075
DrdI GACnn_nn'nnGTC
Cuts at: 2781 4125 2781
Size: 1344 3731
EaeI y'GGCC_r
Cuts at: 227 1368 1583 1763 2660 2834 3225 3252 4597
Size: 1141 215 180 897 174 391 27 1345
Cuts at: 4597 227
Size: 705
Fragments arranged by size:
1345 1141 897 705 391 215 180 174
27
EagI C'GGCC_G
Cuts at: 1583 2660 1583
Size: 1077 3998
EarI CTCTTCn'nnn_
Cuts at: 378 1967 3098 3308 378
Size: 1589 1131 210 2145
Fragments arranged by size:
2145 1589 1131 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 597 702 3283 4025 4171 4885 597
Size: 105 2581 742 146 714 787
Fragments arranged by size:
2581 787 742 714 146 105
EcoICRI GAG'CTC
Cuts at: 153 786 2087 153
Size: 633 1301 3141
Fragments arranged by size:
3141 1301 633
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 315 810 1005 1690 2900 3332 3685 4347
Size: 159 495 195 685 1210 432 353 662
Cuts at: 4347 156
Size: 884
Fragments arranged by size:
1210 884 685 662 495 432 353 195
159
EcoNI CCTnn'n_nnAGG
Cuts at: 469 1220 1300 1885 469
Size: 751 80 585 3659
Fragments arranged by size:
3659 751 585 80
EcoO109I rG'GnC_Cy
Cuts at: 515 542 633 1191 1674 2214 515
Size: 27 91 558 483 540 3376
Fragments arranged by size:
3376 558 540 483 91 27
EcoRI G'AATT_C
Cuts at: 2079 2079
Size: 5075
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3094 3094
Size: 5075
FseI GG_CCGG'CC
Cuts at: 216 216
Size: 5075
FspI TGC'GCA
Cuts at: 2856 2856
Size: 5075
HaeII r_GCGC'y
Cuts at: 133 428 1381 1758 2509 2757 3987 4758 133
Size: 295 953 377 751 248 1230 771 450
Fragments arranged by size:
1230 953 771 751 450 377 295 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 383 415 1615 1647 2917 2947 2949 2979 383
Size: 32 1200 32 1270 30 2 30 2479
Fragments arranged by size:
2479 1270 1200 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2114 2114
Size: 5075
KasI G'GCGC_C
Cuts at: 129 424 2505 2753 129
Size: 295 2081 248 2451
Fragments arranged by size:
2451 2081 295 248
KpnI G_GTAC'C
Cuts at: 2095 2095
Size: 5075
MluI A'CGCG_T
Cuts at: 4508 4508
Size: 5075
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1416 1977 3834 4018 1416
Size: 561 1857 184 2473
Fragments arranged by size:
2473 1857 561 184
MscI TGG'CCA
Cuts at: 1370 1765 2836 1370
Size: 395 1071 3609
Fragments arranged by size:
3609 1071 395
MslI CAynn'nnrTG
Cuts at: 1312 3064 3191 1312
Size: 1752 127 3196
Fragments arranged by size:
3196 1752 127
MspA1I CmG'CkG
Cuts at: 189 290 846 939 1043 1081 1636 2186 2500
Size: 101 556 93 104 38 555 550 314
Cuts at: 2500 2860 3644 3889 189
Size: 360 784 245 1375
Fragments arranged by size:
1375 784 556 555 550 360 314 245
104 101 93 38
NaeI GCC'GGC
Cuts at: 214 1344 3256 214
Size: 1130 1912 2033
Fragments arranged by size:
2033 1912 1130
NarI GG'CG_CC
Cuts at: 130 425 2506 2754 130
Size: 295 2081 248 2451
Fragments arranged by size:
2451 2081 295 248
NgoMIV G'CCGG_C
Cuts at: 212 1342 3254 212
Size: 1130 1912 2033
Fragments arranged by size:
2033 1912 1130
NotI GC'GGCC_GC
Cuts at: 1583 1583
Size: 5075
NspI r_CATG'y
Cuts at: 1353 1833 2128 2628 4231 1353
Size: 480 295 500 1603 2197
Fragments arranged by size:
2197 1603 500 480 295
PasI CC'CwG_GG
Cuts at: 1188 1188
Size: 5075
PciI A'CATG_T
Cuts at: 4227 4227
Size: 5075
PflMI CCAn_nnn'nTGG
Cuts at: 1194 1332 1194
Size: 138 4937
PfoI T'CCnGG_A
Cuts at: 164 164
Size: 5075
PmeI GTTT'AAAC
Cuts at: 2074 2074
Size: 5075
PmlI CAC'GTG
Cuts at: 1314 1314
Size: 5075
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3521 3553 3521
Size: 32 5043
PpuMI rG'GwC_Cy
Cuts at: 542 633 542
Size: 91 4984
PshAI GACnn'nnGTC
Cuts at: 1259 1259
Size: 5075
PspOMI G'GGCC_C
Cuts at: 514 817 1682 514
Size: 303 865 3907
Fragments arranged by size:
3907 865 303
PspXI vC'TCGA_Gb
Cuts at: 1217 1217
Size: 5075
PstI C_TGCA'G
Cuts at: 1277 2122 2807 1277
Size: 845 685 3545
Fragments arranged by size:
3545 845 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5075
PvuII CAG'CTG
Cuts at: 939 1081 2500 2860 939
Size: 142 1419 360 3154
Fragments arranged by size:
3154 1419 360 142
RsrII CG'GwC_CG
Cuts at: 3270 3270
Size: 5075
SacI G_AGCT'C
Cuts at: 155 788 2089 155
Size: 633 1301 3141
Fragments arranged by size:
3141 1301 633
SalI G'TCGA_C
Cuts at: 2112 2112
Size: 5075
SapI GCTCTTCn'nnn_
Cuts at: 3098 3308 3098
Size: 210 4865
SbfI CC_TGCA'GG
Cuts at: 2122 2122
Size: 5075
ScaI AGT'ACT
Cuts at: 553 4592 553
Size: 4039 1036
SfcI C'TryA_G
Cuts at: 33 1273 1551 2118 2803 3771 3962 33
Size: 1240 278 567 685 968 191 1146
Fragments arranged by size:
1240 1146 968 685 567 278 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1681 1681
Size: 5075
SfoI GGC'GCC
Cuts at: 131 426 2507 2755 131
Size: 295 2081 248 2451
Fragments arranged by size:
2451 2081 295 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5075
SmaI CCC'GGG
Cuts at: 513 800 1681 1777 2097 513
Size: 287 881 96 320 3491
Fragments arranged by size:
3491 881 320 287 96
SmlI C'TyrA_G
Cuts at: 256 1140 1217 2230 3145 3859 4121 256
Size: 884 77 1013 915 714 262 1210
Fragments arranged by size:
1210 1013 915 884 714 262 77
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5075
SphI G_CATG'C
Cuts at: 2128 2628 2128
Size: 500 4575
StuI AGG'CCT
Cuts at: 1430 1810 2036 1430
Size: 380 226 4469
Fragments arranged by size:
4469 380 226
StyI C'CwwG_G
Cuts at: 2209 2563 2209
Size: 354 4721
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 358 681 817 1402 1887 2045 2658 2791 3250
Size: 323 136 585 485 158 613 133 459
Cuts at: 3250 3459 358
Size: 209 1974
Fragments arranged by size:
1974 613 585 485 459 323 209 158
136 133
TatI w'GTAC_w
Cuts at: 551 4590 551
Size: 4039 1036
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 938 970 4592 4624 938
Size: 32 3622 32 1389
Fragments arranged by size:
3622 1389 32 32
Tth111I GACn'n_nGTC
Cuts at: 2872 2872
Size: 5075
XbaI T'CTAG_A
Cuts at: 2106 2106
Size: 5075
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2570 2570
Size: 5075
XhoI C'TCGA_G
Cuts at: 1217 1217
Size: 5075
XmaI C'CCGG_G
Cuts at: 511 798 1679 1775 2095 511
Size: 287 881 96 320 3491
Fragments arranged by size:
3491 881 320 287 96
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI AloI
AscI AvrII BaeI BfrBI BmtI BsmI BsrGI BssHII
BstEII BstZ17I ClaI CspCI EcoRV FspAI HindIII HpaI
MfeI NcoI NdeI NheI NruI NsiI PacI PsiI
PsrI SacII SanDI SexAI SgrAI SnaBI SrfI SspI
SwaI XmnI ZraI