Restriction map for
pF1KB5147
(Circular) (Six-base) MAPSORT of: pF1KB5147.seq Check: 6257 from: 1 to: 4175
pF1KB5147 4175 bp
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AccI GT'mk_AC
Cuts at: 1213 1213
Size: 4175
Acc65I G'GTAC_C
Cuts at: 481 630 1191 481
Size: 149 561 3465
Fragments arranged by size:
3465 561 149
AclI AA'CG_TT
Cuts at: 4015 4015
Size: 4175
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 2000 2432 3179 3447 184
Size: 1816 432 747 268 912
Fragments arranged by size:
1816 912 747 432 268
AflIII A'CryG_T
Cuts at: 1158 1527 2631 3608 1158
Size: 369 1104 977 1725
Fragments arranged by size:
1725 1104 977 369
AgeI A'CCGG_T
Cuts at: 2563 2563
Size: 4175
AlwNI CAG_nnn'CTG
Cuts at: 402 1480 3047 402
Size: 1078 1567 1530
Fragments arranged by size:
1567 1530 1078
ApaLI G'TGCA_C
Cuts at: 2945 2945
Size: 4175
ApoI r'AATT_y
Cuts at: 1179 3528 3784 1179
Size: 2349 256 1570
Fragments arranged by size:
2349 1570 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4175
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4175
AvaI C'yCGr_G
Cuts at: 1195 1195
Size: 4175
BamHI G'GATC_C
Cuts at: 1200 1200
Size: 4175
BanI G'GyrC_C
Cuts at: 481 630 1191 1605 1853 1888 481
Size: 149 561 414 248 35 2768
Fragments arranged by size:
2768 561 414 248 149 35
BanII G_rGCy'C
Cuts at: 201 1189 2219 201
Size: 988 1030 2157
Fragments arranged by size:
2157 1030 988
BbeI G_GCGC'C
Cuts at: 1609 1857 1609
Size: 248 3927
BbsI GAAGACnn'nnnn_
Cuts at: 1039 1039
Size: 4175
BbvCI CC'TCA_GC
Cuts at: 407 947 407
Size: 540 3635
BciVI GTATCCnnnnn_n'
Cuts at: 796 2073 2834 4175 796
Size: 1277 761 1341 796
Fragments arranged by size:
1341 1277 796 761
BclI T'GATC_A
Cuts at: 1695 1695
Size: 4175
BfrBI ATG'CAT
Cuts at: 868 868
Size: 4175
BglII A'GATC_T
Cuts at: 1690 3727 1690
Size: 2037 2138
BlpI GC'TnA_GC
Cuts at: 1287 1287
Size: 4175
Bme1580I G_kGCm'C
Cuts at: 1800 1893 2949 1800
Size: 93 1056 3026
Fragments arranged by size:
3026 1056 93
BmgBI CAC'GTC
Cuts at: 708 708
Size: 4175
BmrI ACTGGGnnnn_n'
Cuts at: 463 1121 1387 1468 1803 3947 463
Size: 658 266 81 335 2144 691
Fragments arranged by size:
2144 691 658 335 266 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 344 2542 344
Size: 2198 1977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 124 267 314 872 124
Size: 143 47 558 3427
Fragments arranged by size:
3427 558 143 47
Bpu10I CC'TnA_GC
Cuts at: 407 947 407
Size: 540 3635
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1351 2230 2722 3020 1351
Size: 879 492 298 2506
Fragments arranged by size:
2506 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 455 3879 455
Size: 3424 751
BsaAI yAC'GTr
Cuts at: 1159 1528 1159
Size: 369 3806
BsaBI GATnn'nnATC
Cuts at: 6 773 1689 3726 6
Size: 767 916 2037 455
Fragments arranged by size:
2037 916 767 455
BsaHI Gr'CG_yC
Cuts at: 1606 1854 2556 4052 1606
Size: 248 702 1496 1729
Fragments arranged by size:
1729 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1364 1885 2563 2837 2984 3395 1364
Size: 521 678 274 147 411 2144
Fragments arranged by size:
2144 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 623 653 870 885 900 915 623
Size: 30 217 15 15 15 3883
Fragments arranged by size:
3883 217 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 473 598 127
Size: 346 125 3704
Fragments arranged by size:
3704 346 125
BseYI C'CCAG_C
Cuts at: 1600 2358 2935 3431 1600
Size: 758 577 496 2344
Fragments arranged by size:
2344 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1717 1763 2971 104
Size: 1613 46 1208 1308
Fragments arranged by size:
1613 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1189 1967 2157 2949 1189
Size: 778 190 792 2415
Fragments arranged by size:
2415 792 778 190
BsmI GAATG_Cn'
Cuts at: 153 153
Size: 4175
BsmBI CGTCTCn'nnnn_
Cuts at: 139 698 139
Size: 559 3616
Bsp1286I G_dGCh'C
Cuts at: 201 1189 1800 1893 1967 2157 2219 2949 201
Size: 988 611 93 74 190 62 730 1427
Fragments arranged by size:
1427 988 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3395 3395
Size: 4175
BspHI T'CATG_A
Cuts at: 3351 3351
Size: 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1225 1439 1741 2122 1225
Size: 214 302 381 3278
Fragments arranged by size:
3278 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2467 2521 4005 4170 50
Size: 2417 54 1484 165 55
Fragments arranged by size:
2417 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2087 2087
Size: 4175
BsrFI r'CCGG_y
Cuts at: 2173 2354 2563 2173
Size: 181 209 3785
Fragments arranged by size:
3785 209 181
BssSI C'ACGA_G
Cuts at: 2446 2804 2446
Size: 358 3817
BstAPI GCAn_nnn'nTGC
Cuts at: 1552 1552
Size: 4175
BstBI TT'CG_AA
Cuts at: 2536 2536
Size: 4175
BstYI r'GATC_y
Cuts at: 1 984 1200 1667 1690 2025 2271 3283 3369
Size: 983 216 467 23 335 246 1012 86
Cuts at: 3369 3381 3727 1
Size: 12 346 449
Fragments arranged by size:
1012 983 467 449 346 335 246 216
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 115 115
Size: 4175
BtgI C'CryG_G
Cuts at: 182 585 182
Size: 403 3772
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2125 2306 2469 2125
Size: 181 163 3831
Fragments arranged by size:
3831 181 163
BtsI GCAGTG_nn'
Cuts at: 504 1418 504
Size: 914 3261
DraI TTT'AAA
Cuts at: 734 1174 734
Size: 440 3735
DraIII CAC_nnn'GTG
Cuts at: 183 183
Size: 4175
DrdI GACnn_nn'nnGTC
Cuts at: 1881 2739 1881
Size: 858 3317
EaeI y'GGCC_r
Cuts at: 492 1010 1760 1934 2325 2352 3697 492
Size: 518 750 174 391 27 1345 970
Fragments arranged by size:
1345 970 750 518 391 174 27
EagI C'GGCC_G
Cuts at: 1760 1760
Size: 4175
EarI CTCTTCn'nnn_
Cuts at: 678 1019 2198 2408 678
Size: 341 1179 210 2445
Fragments arranged by size:
2445 1179 341 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2383 2693 2839 3985 2383
Size: 310 146 1146 2573
Fragments arranged by size:
2573 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1187 1187
Size: 4175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 124 184 267 314 872 2000 2432 3179 3447
Size: 60 83 47 558 1128 432 747 268
Cuts at: 3447 124
Size: 852
Fragments arranged by size:
1128 852 747 558 432 268 83 60
47
EcoO109I rG'GnC_Cy
Cuts at: 1314 1314
Size: 4175
EcoRI G'AATT_C
Cuts at: 1179 1179
Size: 4175
EcoRV GAT'ATC
Cuts at: 747 747
Size: 4175
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2194 2194
Size: 4175
FspI TGC'GCA
Cuts at: 1956 1956
Size: 4175
HaeII r_GCGC'y
Cuts at: 1609 1857 2879 3858 1609
Size: 248 1022 979 1926
Fragments arranged by size:
1926 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 354 386 770 802 2017 2047 2049 2079 354
Size: 32 384 32 1215 30 2 30 2450
Fragments arranged by size:
2450 1215 384 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1214 1214
Size: 4175
HindIII A'AGCT_T
Cuts at: 1071 1071
Size: 4175
KasI G'GCGC_C
Cuts at: 1605 1853 1605
Size: 248 3927
KpnI G_GTAC'C
Cuts at: 485 634 1195 485
Size: 149 561 3465
Fragments arranged by size:
3465 561 149
MluI A'CGCG_T
Cuts at: 3608 3608
Size: 4175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2846 3030 2846
Size: 184 3991
MscI TGG'CCA
Cuts at: 494 1012 1936 494
Size: 518 924 2733
Fragments arranged by size:
2733 924 518
MslI CAynn'nnrTG
Cuts at: 2164 2291 2164
Size: 127 4048
MspA1I CmG'CkG
Cuts at: 247 443 1286 1600 1960 2973 3218 247
Size: 196 843 314 360 1013 245 1204
Fragments arranged by size:
1204 1013 843 360 314 245 196
NaeI GCC'GGC
Cuts at: 2356 2356
Size: 4175
NarI GG'CG_CC
Cuts at: 1606 1854 1606
Size: 248 3927
NcoI C'CATG_G
Cuts at: 585 585
Size: 4175
NgoMIV G'CCGG_C
Cuts at: 2354 2354
Size: 4175
NsiI A_TGCA'T
Cuts at: 870 870
Size: 4175
NspI r_CATG'y
Cuts at: 939 1228 1728 2635 939
Size: 289 500 907 2479
Fragments arranged by size:
2479 907 500 289
PciI A'CATG_T
Cuts at: 2631 2631
Size: 4175
PflMI CCAn_nnn'nTGG
Cuts at: 183 402 471 183
Size: 219 69 3887
Fragments arranged by size:
3887 219 69
PfoI T'CCnGG_A
Cuts at: 652 652
Size: 4175
PmeI GTTT'AAAC
Cuts at: 1174 1174
Size: 4175
PmlI CAC'GTG
Cuts at: 1159 1159
Size: 4175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3314 3346 3314
Size: 32 4143
PstI C_TGCA'G
Cuts at: 1222 1907 1222
Size: 685 3490
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4175
PvuII CAG'CTG
Cuts at: 443 1600 1960 443
Size: 1157 360 2658
Fragments arranged by size:
2658 1157 360
RsrII CG'GwC_CG
Cuts at: 2370 2370
Size: 4175
SacI G_AGCT'C
Cuts at: 1189 1189
Size: 4175
SalI G'TCGA_C
Cuts at: 1212 1212
Size: 4175
SapI GCTCTTCn'nnn_
Cuts at: 2198 2408 2198
Size: 210 3965
SbfI CC_TGCA'GG
Cuts at: 1222 1222
Size: 4175
ScaI AGT'ACT
Cuts at: 3692 3692
Size: 4175
SfcI C'TryA_G
Cuts at: 33 438 619 1037 1218 1903 2896 3087 33
Size: 405 181 418 181 685 993 191 1121
Fragments arranged by size:
1121 993 685 418 405 191 181 181
SfoI GGC'GCC
Cuts at: 1607 1855 1607
Size: 248 3927
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4175
SmaI CCC'GGG
Cuts at: 1197 1197
Size: 4175
SmlI C'TyrA_G
Cuts at: 1330 2245 2737 2999 1330
Size: 915 492 262 2506
Fragments arranged by size:
2506 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4175
SphI G_CATG'C
Cuts at: 1228 1728 1228
Size: 500 3675
StyI C'CwwG_G
Cuts at: 585 1309 1663 585
Size: 724 354 3097
Fragments arranged by size:
3097 724 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1758 1891 2350 2559 1758
Size: 133 459 209 3374
Fragments arranged by size:
3374 459 209 133
TatI w'GTAC_w
Cuts at: 3690 3690
Size: 4175
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 885 917 3692 3724 885
Size: 32 2775 32 1336
Fragments arranged by size:
2775 1336 32 32
Tth111I GACn'n_nGTC
Cuts at: 1972 1972
Size: 4175
XbaI T'CTAG_A
Cuts at: 1206 1206
Size: 4175
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 399 650 1670 399
Size: 251 1020 2904
Fragments arranged by size:
2904 1020 251
XmaI C'CCGG_G
Cuts at: 1195 1195
Size: 4175
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PmlI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BglI BmtI BsgI
BsiWI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
EcoNI FseI FspAI HpaI MfeI NdeI NheI NotI
NruI PacI PasI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI