Restriction map for
pF1KB5144
(Circular) (Six-base) MAPSORT of: pF1KB5144.seq Check: 2456 from: 1 to: 5303
pF1KB5144 5303 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 2341 2341
Size: 5303
Acc65I G'GTAC_C
Cuts at: 2319 2319
Size: 5303
AclI AA'CG_TT
Cuts at: 5143 5143
Size: 5303
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 1952 3128 3560 3913 4575 293
Size: 1659 1176 432 353 662 1021
Fragments arranged by size:
1659 1176 1021 662 432 353
AflIII A'CryG_T
Cuts at: 959 2655 4455 4736 959
Size: 1696 1800 281 1526
Fragments arranged by size:
1800 1696 1526 281
AlwNI CAG_nnn'CTG
Cuts at: 2008 2290 2608 4046 2008
Size: 282 318 1438 3265
Fragments arranged by size:
3265 1438 318 282
ApaLI G'TGCA_C
Cuts at: 4141 4141
Size: 5303
ApoI r'AATT_y
Cuts at: 479 1218 1237 1592 1936 2172 2307 4656 4912
Size: 739 19 355 344 236 135 2349 256
Cuts at: 4912 479
Size: 870
Fragments arranged by size:
2349 870 739 355 344 256 236 135
19
AseI AT'TA_AT
Cuts at: 20 20
Size: 5303
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5303
AvaI C'yCGr_G
Cuts at: 2323 2323
Size: 5303
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 242 275 242
Size: 33 5270
BamHI G'GATC_C
Cuts at: 2206 2328 2206
Size: 122 5181
BanI G'GyrC_C
Cuts at: 2319 2733 2981 3016 2319
Size: 414 248 35 4606
Fragments arranged by size:
4606 414 248 35
BanII G_rGCy'C
Cuts at: 995 2317 3347 995
Size: 1322 1030 2951
Fragments arranged by size:
2951 1322 1030
BbeI G_GCGC'C
Cuts at: 2737 2985 2737
Size: 248 5055
BbsI GAAGACnn'nnnn_
Cuts at: 194 845 1606 1778 194
Size: 651 761 172 3719
Fragments arranged by size:
3719 761 651 172
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 2142 2176 90
Size: 34 2018 34 3217
Fragments arranged by size:
3217 2018 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2214 3201 4257 5303 2214
Size: 987 1056 1046 2214
Fragments arranged by size:
2214 1056 1046 987
BclI T'GATC_A
Cuts at: 836 2823 836
Size: 1987 3316
BfrBI ATG'CAT
Cuts at: 1208 1208
Size: 5303
BglII A'GATC_T
Cuts at: 2818 4855 2818
Size: 2037 3266
BlpI GC'TnA_GC
Cuts at: 1428 2415 1428
Size: 987 4316
Bme1580I G_kGCm'C
Cuts at: 2928 3021 4145 2928
Size: 93 1124 4086
Fragments arranged by size:
4086 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 535 1996 2515 2596 2931 5075 535
Size: 1461 519 81 335 2144 763
Fragments arranged by size:
2144 1461 763 519 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3670 3670
Size: 5303
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 618 1009 1023 618
Size: 391 14 4898
Fragments arranged by size:
4898 391 14
Bpu10I CC'TnA_GC
Cuts at: 971 971
Size: 5303
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1004 2479 3358 4072 4370 1004
Size: 1475 879 714 298 1937
Fragments arranged by size:
1937 1475 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5007 5007
Size: 5303
BsaAI yAC'GTr
Cuts at: 2656 2656
Size: 5303
BsaBI GATnn'nnATC
Cuts at: 6 793 2817 4854 6
Size: 787 2024 2037 455
Fragments arranged by size:
2037 2024 787 455
BsaHI Gr'CG_yC
Cuts at: 2734 2982 3684 5180 2734
Size: 248 702 1496 2857
Fragments arranged by size:
2857 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2492 3013 3691 4102 4249 4523 2492
Size: 521 678 411 147 274 3272
Fragments arranged by size:
3272 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1829 1829
Size: 5303
BseYI C'CCAG_C
Cuts at: 1788 1801 2728 3486 4151 4559 1788
Size: 13 927 758 665 408 2532
Fragments arranged by size:
2532 927 758 665 408 13
BsiEI CG_ry'CG
Cuts at: 104 2845 2891 4121 104
Size: 2741 46 1230 1286
Fragments arranged by size:
2741 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2317 3095 3285 4145 2317
Size: 778 190 860 3475
Fragments arranged by size:
3475 860 778 190
BsmI GAATG_Cn'
Cuts at: 845 922 950 845
Size: 77 28 5198
Fragments arranged by size:
5198 77 28
BsmBI CGTCTCn'nnnn_
Cuts at: 1219 1219
Size: 5303
Bsp1286I G_dGCh'C
Cuts at: 995 2317 2928 3021 3095 3285 3347 4145 995
Size: 1322 611 93 74 190 62 798 2153
Fragments arranged by size:
2153 1322 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 1232 3735 1232
Size: 2503 2800
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2353 2567 2869 3250 2353
Size: 214 302 381 4406
Fragments arranged by size:
4406 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3595 3649 5133 5298 50
Size: 3545 54 1484 165 55
Fragments arranged by size:
3545 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1058 1855 3215 1058
Size: 797 1360 3146
Fragments arranged by size:
3146 1360 797
BsrFI r'CCGG_y
Cuts at: 1423 3301 3482 1423
Size: 1878 181 3244
Fragments arranged by size:
3244 1878 181
BssSI C'ACGA_G
Cuts at: 3574 4282 3574
Size: 708 4595
BstAPI GCAn_nnn'nTGC
Cuts at: 636 2680 636
Size: 2044 3259
BstBI TT'CG_AA
Cuts at: 3664 3664
Size: 5303
BstYI r'GATC_y
Cuts at: 1 268 2206 2328 2795 2818 3153 3399 3705
Size: 267 1938 122 467 23 335 246 306
Cuts at: 3705 3717 3803 4855 1
Size: 12 86 1052 449
Fragments arranged by size:
1938 1052 467 449 335 306 267 246
122 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1035 1035
Size: 5303
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1468 2135 3253 3434 3597 1468
Size: 667 1118 181 163 3174
Fragments arranged by size:
3174 1118 667 181 163
BtsI GCAGTG_nn'
Cuts at: 1696 2546 1696
Size: 850 4453
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1139 1174 1139
Size: 35 5268
DraI TTT'AAA
Cuts at: 932 2302 932
Size: 1370 3933
DrdI GACnn_nn'nnGTC
Cuts at: 3009 4353 3009
Size: 1344 3959
EaeI y'GGCC_r
Cuts at: 234 2888 3062 3453 3480 4825 234
Size: 2654 174 391 27 1345 712
Fragments arranged by size:
2654 1345 712 391 174 27
EagI C'GGCC_G
Cuts at: 2888 2888
Size: 5303
EarI CTCTTCn'nnn_
Cuts at: 236 1349 1373 1391 3326 3536 236
Size: 1113 24 18 1935 210 2003
Fragments arranged by size:
2003 1935 1113 210 24 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3511 4253 4399 5113 3511
Size: 742 146 714 3701
Fragments arranged by size:
3701 742 714 146
EcoICRI GAG'CTC
Cuts at: 2315 2315
Size: 5303
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 618 1009 1023 1952 3128 3560 3913 4575
Size: 325 391 14 929 1176 432 353 662
Cuts at: 4575 293
Size: 1021
Fragments arranged by size:
1176 1021 929 662 432 391 353 325
14
EcoO109I rG'GnC_Cy
Cuts at: 2091 2442 2091
Size: 351 4952
EcoRI G'AATT_C
Cuts at: 479 2307 479
Size: 1828 3475
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 193 311 1612 3322 193
Size: 118 1301 1710 2174
Fragments arranged by size:
2174 1710 1301 118
FspI TGC'GCA
Cuts at: 3084 3084
Size: 5303
HaeII r_GCGC'y
Cuts at: 2737 2985 4215 4986 2737
Size: 248 1230 771 3054
Fragments arranged by size:
3054 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 260 292 1912 1944 2075 2107 2149 2151 2181
Size: 32 1620 32 131 32 42 2 30
Cuts at: 2181 2183 3145 3175 3177 3207 260
Size: 2 962 30 2 30 2356
Fragments arranged by size:
2356 1620 962 131 42 32 32 32
30 30 30 2 2 2
HincII GTy'rAC
Cuts at: 2342 2342
Size: 5303
HindIII A'AGCT_T
Cuts at: 973 973
Size: 5303
KasI G'GCGC_C
Cuts at: 2733 2981 2733
Size: 248 5055
KpnI G_GTAC'C
Cuts at: 2323 2323
Size: 5303
MfeI C'AATT_G
Cuts at: 941 941
Size: 5303
MluI A'CGCG_T
Cuts at: 4736 4736
Size: 5303
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 312 1975 4062 4246 312
Size: 1663 2087 184 1369
Fragments arranged by size:
2087 1663 1369 184
MscI TGG'CCA
Cuts at: 236 3064 236
Size: 2828 2475
MslI CAynn'nnrTG
Cuts at: 375 1103 3292 3419 375
Size: 728 2189 127 2259
Fragments arranged by size:
2259 2189 728 127
MspA1I CmG'CkG
Cuts at: 196 2414 2728 3088 3872 4117 196
Size: 2218 314 360 784 245 1382
Fragments arranged by size:
2218 1382 784 360 314 245
NaeI GCC'GGC
Cuts at: 3484 3484
Size: 5303
NarI GG'CG_CC
Cuts at: 2734 2982 2734
Size: 248 5055
NdeI CA'TA_TG
Cuts at: 891 891
Size: 5303
NgoMIV G'CCGG_C
Cuts at: 3482 3482
Size: 5303
NsiI A_TGCA'T
Cuts at: 1210 1210
Size: 5303
NspI r_CATG'y
Cuts at: 963 2356 2856 4459 963
Size: 1393 500 1603 1807
Fragments arranged by size:
1807 1603 1393 500
PciI A'CATG_T
Cuts at: 959 4455 959
Size: 3496 1807
PflMI CCAn_nnn'nTGG
Cuts at: 1001 2176 1001
Size: 1175 4128
PmeI GTTT'AAAC
Cuts at: 2302 2302
Size: 5303
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3749 3781 3749
Size: 32 5271
PpuMI rG'GwC_Cy
Cuts at: 2091 2091
Size: 5303
PstI C_TGCA'G
Cuts at: 1308 2350 3035 1308
Size: 1042 685 3576
Fragments arranged by size:
3576 1042 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5303
PvuII CAG'CTG
Cuts at: 196 2728 3088 196
Size: 2532 360 2411
Fragments arranged by size:
2532 2411 360
RsrII CG'GwC_CG
Cuts at: 3498 3498
Size: 5303
SacI G_AGCT'C
Cuts at: 2317 2317
Size: 5303
SalI G'TCGA_C
Cuts at: 2340 2340
Size: 5303
SapI GCTCTTCn'nnn_
Cuts at: 1373 1391 3326 3536 1373
Size: 18 1935 210 3140
Fragments arranged by size:
3140 1935 210 18
SbfI CC_TGCA'GG
Cuts at: 2350 2350
Size: 5303
ScaI AGT'ACT
Cuts at: 4820 4820
Size: 5303
SfcI C'TryA_G
Cuts at: 33 341 1304 2009 2346 3031 3999 4190 33
Size: 308 963 705 337 685 968 191 1146
Fragments arranged by size:
1146 968 963 705 685 337 308 191
SfoI GGC'GCC
Cuts at: 2735 2983 2735
Size: 248 5055
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5303
SmaI CCC'GGG
Cuts at: 2325 2325
Size: 5303
SmlI C'TyrA_G
Cuts at: 1019 2458 3373 4087 4349 1019
Size: 1439 915 714 262 1973
Fragments arranged by size:
1973 1439 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5303
SphI G_CATG'C
Cuts at: 2356 2856 2356
Size: 500 4803
SspI AAT'ATT
Cuts at: 747 1731 2187 747
Size: 984 456 3863
Fragments arranged by size:
3863 984 456
StuI AGG'CCT
Cuts at: 1520 1520
Size: 5303
StyI C'CwwG_G
Cuts at: 640 995 1521 2437 2791 640
Size: 355 526 916 354 3152
Fragments arranged by size:
3152 916 526 355 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2886 3019 3478 3687 2886
Size: 133 459 209 4502
Fragments arranged by size:
4502 459 209 133
TatI w'GTAC_w
Cuts at: 4818 4818
Size: 5303
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4820 4852 4820
Size: 32 5271
Tth111I GACn'n_nGTC
Cuts at: 3100 3100
Size: 5303
XbaI T'CTAG_A
Cuts at: 2334 2334
Size: 5303
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 801 2798 801
Size: 1997 3306
XmaI C'CCGG_G
Cuts at: 2323 2323
Size: 5303
XmnI GAAnn'nnTTC
Cuts at: 1358 1937 1358
Size: 579 4724
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NsiI NspI PciI PflMI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BbvCI BglI BmgBI BmtI
BsaXI BsgI BsiWI BspEI BsrGI BssHII BstEII BstXI
BstZ17I BtgI ClaI DraIII EcoNI EcoRV FseI FspAI
HpaI NcoI NheI NotI NruI PacI PasI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI