Restriction map for
pF1KB5142
(Circular) (Six-base) MAPSORT of: pF1KB5142.seq Check: 9271 from: 1 to: 4406
pF1KB5142 4406 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 1444 1444
Size: 4406
Acc65I G'GTAC_C
Cuts at: 1043 1422 1043
Size: 379 4027
AclI AA'CG_TT
Cuts at: 4246 4246
Size: 4406
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 923 971 2231 2663 3410 3678 923
Size: 48 1260 432 747 268 1651
Fragments arranged by size:
1651 1260 747 432 268 48
AflIII A'CryG_T
Cuts at: 368 1758 2862 3839 368
Size: 1390 1104 977 935
Fragments arranged by size:
1390 1104 977 935
AgeI A'CCGG_T
Cuts at: 2794 2794
Size: 4406
AlwNI CAG_nnn'CTG
Cuts at: 1711 3278 1711
Size: 1567 2839
ApaI G_GGCC'C
Cuts at: 937 937
Size: 4406
ApaLI G'TGCA_C
Cuts at: 3176 3176
Size: 4406
ApoI r'AATT_y
Cuts at: 391 1410 3759 4015 391
Size: 1019 2349 256 782
Fragments arranged by size:
2349 1019 782 256
AseI AT'TA_AT
Cuts at: 20 671 20
Size: 651 3755
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4406
AvaI C'yCGr_G
Cuts at: 1426 1426
Size: 4406
BamHI G'GATC_C
Cuts at: 1431 1431
Size: 4406
BanI G'GyrC_C
Cuts at: 998 1043 1422 1836 2084 2119 998
Size: 45 379 414 248 35 3285
Fragments arranged by size:
3285 414 379 248 45 35
BanII G_rGCy'C
Cuts at: 937 1420 2450 937
Size: 483 1030 2893
Fragments arranged by size:
2893 1030 483
BbeI G_GCGC'C
Cuts at: 1002 1840 2088 1002
Size: 838 248 3320
Fragments arranged by size:
3320 838 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 342 376 1375 1409 342
Size: 34 999 34 3339
Fragments arranged by size:
3339 999 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 272 636 2304 3065 4406 272
Size: 364 1668 761 1341 272
Fragments arranged by size:
1668 1341 761 364 272
BclI T'GATC_A
Cuts at: 1926 1926
Size: 4406
BfrBI ATG'CAT
Cuts at: 1080 1080
Size: 4406
BglII A'GATC_T
Cuts at: 1921 3958 1921
Size: 2037 2369
BlpI GC'TnA_GC
Cuts at: 1088 1518 1088
Size: 430 3976
Bme1580I G_kGCm'C
Cuts at: 937 2031 2124 3180 937
Size: 1094 93 1056 2163
Fragments arranged by size:
2163 1094 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 297 723 1618 1699 2034 4178 297
Size: 426 895 81 335 2144 525
Fragments arranged by size:
2144 895 525 426 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2773 2773
Size: 4406
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1582 2461 2953 3251 1582
Size: 879 492 298 2737
Fragments arranged by size:
2737 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4110 4110
Size: 4406
BsaAI yAC'GTr
Cuts at: 1759 1759
Size: 4406
BsaBI GATnn'nnATC
Cuts at: 6 1920 3957 6
Size: 1914 2037 455
Fragments arranged by size:
2037 1914 455
BsaHI Gr'CG_yC
Cuts at: 747 999 1837 2085 2787 4283 747
Size: 252 838 248 702 1496 870
Fragments arranged by size:
1496 870 838 702 252 248
BsaWI w'CCGG_w
Cuts at: 1595 2116 2794 3068 3215 3626 1595
Size: 521 678 274 147 411 2375
Fragments arranged by size:
2375 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 668 715 832 668
Size: 47 117 4242
Fragments arranged by size:
4242 117 47
BseYI C'CCAG_C
Cuts at: 288 1831 2589 3166 3662 288
Size: 1543 758 577 496 1032
Fragments arranged by size:
1543 1032 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 504 504
Size: 4406
BsiEI CG_ry'CG
Cuts at: 104 1948 1994 3202 104
Size: 1844 46 1208 1308
Fragments arranged by size:
1844 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 297 1420 2198 2388 3180 297
Size: 1123 778 190 792 1523
Fragments arranged by size:
1523 1123 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 297 937 1420 2031 2124 2198 2388 2450 3180
Size: 640 483 611 93 74 190 62 730
Cuts at: 3180 297
Size: 1523
Fragments arranged by size:
1523 730 640 611 483 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3626 3626
Size: 4406
BspHI T'CATG_A
Cuts at: 345 3582 345
Size: 3237 1169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1456 1670 1972 2353 1456
Size: 214 302 381 3509
Fragments arranged by size:
3509 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2698 2752 4236 4401 50
Size: 2648 54 1484 165 55
Fragments arranged by size:
2648 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 201 1082 2318 201
Size: 881 1236 2289
Fragments arranged by size:
2289 1236 881
BsrFI r'CCGG_y
Cuts at: 2404 2585 2794 2404
Size: 181 209 4016
Fragments arranged by size:
4016 209 181
BssSI C'ACGA_G
Cuts at: 2677 3035 2677
Size: 358 4048
BstAPI GCAn_nnn'nTGC
Cuts at: 1783 1783
Size: 4406
BstBI TT'CG_AA
Cuts at: 1324 2767 1324
Size: 1443 2963
BstYI r'GATC_y
Cuts at: 1 340 1431 1898 1921 2256 2502 3514 3600
Size: 339 1091 467 23 335 246 1012 86
Cuts at: 3600 3612 3958 1
Size: 12 346 449
Fragments arranged by size:
1091 1012 467 449 346 339 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 929 929
Size: 4406
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2356 2537 2700 2356
Size: 181 163 4062
Fragments arranged by size:
4062 181 163
BtsI GCAGTG_nn'
Cuts at: 639 1649 639
Size: 1010 3396
ClaI AT'CG_AT
Cuts at: 1385 1385
Size: 4406
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1097 1132 1097
Size: 35 4371
DraI TTT'AAA
Cuts at: 1405 1405
Size: 4406
DraIII CAC_nnn'GTG
Cuts at: 793 793
Size: 4406
DrdI GACnn_nn'nnGTC
Cuts at: 1359 2112 2970 1359
Size: 753 858 2795
Fragments arranged by size:
2795 858 753
EaeI y'GGCC_r
Cuts at: 1991 2165 2556 2583 3928 1991
Size: 174 391 27 1345 2469
Fragments arranged by size:
2469 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1991 1991
Size: 4406
EarI CTCTTCn'nnn_
Cuts at: 692 857 1145 2429 2639 692
Size: 165 288 1284 210 2459
Fragments arranged by size:
2459 1284 288 210 165
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2614 2924 3070 4216 2614
Size: 310 146 1146 2804
Fragments arranged by size:
2804 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1418 1418
Size: 4406
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 923 971 2231 2663 3410 3678 923
Size: 48 1260 432 747 268 1651
Fragments arranged by size:
1651 1260 747 432 268 48
EcoO109I rG'GnC_Cy
Cuts at: 934 1545 934
Size: 611 3795
EcoRI G'AATT_C
Cuts at: 1410 1410
Size: 4406
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1108 2425 1108
Size: 1317 3089
FspI TGC'GCA
Cuts at: 2187 2187
Size: 4406
HaeII r_GCGC'y
Cuts at: 244 1002 1840 2088 3110 4089 244
Size: 758 838 248 1022 979 561
Fragments arranged by size:
1022 979 838 758 561 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 150 182 1255 1287 2248 2278 2280 2310 150
Size: 32 1073 32 961 30 2 30 2246
Fragments arranged by size:
2246 1073 961 32 32 30 30 2
HincII GTy'rAC
Cuts at: 123 219 228 1445 123
Size: 96 9 1217 3084
Fragments arranged by size:
3084 1217 96 9
KasI G'GCGC_C
Cuts at: 998 1836 2084 998
Size: 838 248 3320
Fragments arranged by size:
3320 838 248
KpnI G_GTAC'C
Cuts at: 1047 1426 1047
Size: 379 4027
MfeI C'AATT_G
Cuts at: 812 812
Size: 4406
MluI A'CGCG_T
Cuts at: 3839 3839
Size: 4406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 280 353 1151 3077 3261 280
Size: 73 798 1926 184 1425
Fragments arranged by size:
1926 1425 798 184 73
MscI TGG'CCA
Cuts at: 2167 2167
Size: 4406
MslI CAynn'nnrTG
Cuts at: 1312 2395 2522 1312
Size: 1083 127 3196
Fragments arranged by size:
3196 1083 127
MspA1I CmG'CkG
Cuts at: 1166 1517 1831 2191 3204 3449 1166
Size: 351 314 360 1013 245 2123
Fragments arranged by size:
2123 1013 360 351 314 245
NaeI GCC'GGC
Cuts at: 2587 2587
Size: 4406
NarI GG'CG_CC
Cuts at: 999 1837 2085 999
Size: 838 248 3320
Fragments arranged by size:
3320 838 248
NcoI C'CATG_G
Cuts at: 929 929
Size: 4406
NgoMIV G'CCGG_C
Cuts at: 2585 2585
Size: 4406
NsiI A_TGCA'T
Cuts at: 1082 1082
Size: 4406
NspI r_CATG'y
Cuts at: 372 439 498 1459 1959 2866 372
Size: 67 59 961 500 907 1912
Fragments arranged by size:
1912 961 907 500 67 59
PciI A'CATG_T
Cuts at: 368 2862 368
Size: 2494 1912
PmeI GTTT'AAAC
Cuts at: 1405 1405
Size: 4406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3545 3577 3545
Size: 32 4374
PsiI TTA'TAA
Cuts at: 1243 1243
Size: 4406
PspOMI G'GGCC_C
Cuts at: 933 933
Size: 4406
PstI C_TGCA'G
Cuts at: 1453 2138 1453
Size: 685 3721
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4406
PvuII CAG'CTG
Cuts at: 1166 1831 2191 1166
Size: 665 360 3381
Fragments arranged by size:
3381 665 360
RsrII CG'GwC_CG
Cuts at: 2601 2601
Size: 4406
SacI G_AGCT'C
Cuts at: 1420 1420
Size: 4406
SalI G'TCGA_C
Cuts at: 1443 1443
Size: 4406
SapI GCTCTTCn'nnn_
Cuts at: 2429 2639 2429
Size: 210 4196
SbfI CC_TGCA'GG
Cuts at: 1453 1453
Size: 4406
ScaI AGT'ACT
Cuts at: 3923 3923
Size: 4406
SfcI C'TryA_G
Cuts at: 33 1449 2134 3127 3318 33
Size: 1416 685 993 191 1121
Fragments arranged by size:
1416 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1000 1838 2086 1000
Size: 838 248 3320
Fragments arranged by size:
3320 838 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4406
SmaI CCC'GGG
Cuts at: 1428 1428
Size: 4406
SmlI C'TyrA_G
Cuts at: 1561 2476 2968 3230 1561
Size: 915 492 262 2737
Fragments arranged by size:
2737 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4406
SphI G_CATG'C
Cuts at: 498 1459 1959 498
Size: 961 500 2945
Fragments arranged by size:
2945 961 500
SspI AAT'ATT
Cuts at: 274 274
Size: 4406
StuI AGG'CCT
Cuts at: 1180 1180
Size: 4406
StyI C'CwwG_G
Cuts at: 176 929 1540 1894 176
Size: 753 611 354 2688
Fragments arranged by size:
2688 753 611 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1989 2122 2581 2790 1989
Size: 133 459 209 3605
Fragments arranged by size:
3605 459 209 133
TatI w'GTAC_w
Cuts at: 3921 3921
Size: 4406
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3923 3955 3923
Size: 32 4374
Tth111I GACn'n_nGTC
Cuts at: 2203 2203
Size: 4406
XbaI T'CTAG_A
Cuts at: 1437 1437
Size: 4406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1244 1901 1244
Size: 657 3749
XmaI C'CCGG_G
Cuts at: 1426 1426
Size: 4406
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PmeI PpiI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI
HindIII HpaI NdeI NheI NotI NruI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
XmnI ZraI