Restriction map for
pF1KB5140
(Circular) (Six-base) MAPSORT of: pF1KB5140.seq Check: 8407 from: 1 to: 4292
pF1KB5140 4292 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 992 1330 992
Size: 338 3954
Acc65I G'GTAC_C
Cuts at: 1308 1308
Size: 4292
AclI AA'CG_TT
Cuts at: 4132 4132
Size: 4292
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2117 2549 2902 3564 2117
Size: 432 353 662 2845
Fragments arranged by size:
2845 662 432 353
AfeI AGC'GCT
Cuts at: 175 175
Size: 4292
AflIII A'CryG_T
Cuts at: 1644 3444 3725 1644
Size: 1800 281 2211
Fragments arranged by size:
2211 1800 281
AleI CACnn'nnGTG
Cuts at: 262 1024 1101 262
Size: 762 77 3453
Fragments arranged by size:
3453 762 77
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 535 535
Size: 4292
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 748 780 748
Size: 32 4260
AlwNI CAG_nnn'CTG
Cuts at: 753 1129 1597 3035 753
Size: 376 468 1438 2010
Fragments arranged by size:
2010 1438 468 376
ApaLI G'TGCA_C
Cuts at: 3130 3130
Size: 4292
ApoI r'AATT_y
Cuts at: 1296 3645 3901 1296
Size: 2349 256 1687
Fragments arranged by size:
2349 1687 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4292
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4292
AvaI C'yCGr_G
Cuts at: 136 505 901 934 1112 1312 136
Size: 369 396 33 178 200 3116
Fragments arranged by size:
3116 396 369 200 178 33
BamHI G'GATC_C
Cuts at: 1317 1317
Size: 4292
BanI G'GyrC_C
Cuts at: 158 278 334 479 659 1089 1308 1722 1970
Size: 120 56 145 180 430 219 414 248
Cuts at: 1970 2005 158
Size: 35 2445
Fragments arranged by size:
2445 430 414 248 219 180 145 120
56 35
BanII G_rGCy'C
Cuts at: 547 982 1306 2336 547
Size: 435 324 1030 2503
Fragments arranged by size:
2503 1030 435 324
BbeI G_GCGC'C
Cuts at: 162 282 338 663 1726 1974 162
Size: 120 56 325 1063 248 2480
Fragments arranged by size:
2480 1063 325 248 120 56
BbsI GAAGACnn'nnnn_
Cuts at: 615 615
Size: 4292
BbvCI CC'TCA_GC
Cuts at: 283 790 283
Size: 507 3785
BciVI GTATCCnnnnn_n'
Cuts at: 2190 3246 4292 2190
Size: 1056 1046 2190
Fragments arranged by size:
2190 1056 1046
BclI T'GATC_A
Cuts at: 1812 1812
Size: 4292
BglI GCCn_nnn'nGGC
Cuts at: 156 509 156
Size: 353 3939
BglII A'GATC_T
Cuts at: 587 1807 3844 587
Size: 1220 2037 1035
Fragments arranged by size:
2037 1220 1035
BlpI GC'TnA_GC
Cuts at: 1404 1404
Size: 4292
Bme1580I G_kGCm'C
Cuts at: 482 505 900 1094 1917 2010 3134 482
Size: 23 395 194 823 93 1124 1640
Fragments arranged by size:
1640 1124 823 395 194 93 23
BmgBI CAC'GTC
Cuts at: 1022 1022
Size: 4292
BmrI ACTGGGnnnn_n'
Cuts at: 1504 1585 1920 4064 1504
Size: 81 335 2144 1732
Fragments arranged by size:
2144 1732 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2659 2659
Size: 4292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 403 775 1057 394
Size: 9 372 282 3629
Fragments arranged by size:
3629 372 282 9
Bpu10I CC'TnA_GC
Cuts at: 283 790 283
Size: 507 3785
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1468 2347 3061 3359 1468
Size: 879 714 298 2401
Fragments arranged by size:
2401 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 448 454 3996 448
Size: 6 3542 744
Fragments arranged by size:
3542 744 6
BsaAI yAC'GTr
Cuts at: 180 1645 180
Size: 1465 2827
BsaBI GATnn'nnATC
Cuts at: 6 1148 1806 3843 6
Size: 1142 658 2037 455
Fragments arranged by size:
2037 1142 658 455
BsaHI Gr'CG_yC
Cuts at: 159 279 335 400 660 863 886 1723 1971
Size: 120 56 65 260 203 23 837 248
Cuts at: 1971 2673 4169 159
Size: 702 1496 282
Fragments arranged by size:
1496 837 702 282 260 248 203 120
65 56 23
BsaWI w'CCGG_w
Cuts at: 1481 2002 2680 3091 3238 3512 1481
Size: 521 678 411 147 274 2261
Fragments arranged by size:
2261 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 679 709 748 778 679
Size: 30 39 30 4193
Fragments arranged by size:
4193 39 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 160 618 687 1207 1210 160
Size: 458 69 520 3 3242
Fragments arranged by size:
3242 520 458 69 3
BseYI C'CCAG_C
Cuts at: 1168 1228 1717 2475 3140 3548 1168
Size: 60 489 758 665 408 1912
Fragments arranged by size:
1912 758 665 489 408 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 760 1125 226
Size: 534 365 3393
Fragments arranged by size:
3393 534 365
BsiEI CG_ry'CG
Cuts at: 104 168 363 819 1834 1880 3110 104
Size: 64 195 456 1015 46 1230 1286
Fragments arranged by size:
1286 1230 1015 456 195 64 46
BsiHKAI G_wGCw'C
Cuts at: 380 982 1113 1306 2084 2274 3134 380
Size: 602 131 193 778 190 860 1538
Fragments arranged by size:
1538 860 778 602 193 190 131
BsmBI CGTCTCn'nnnn_
Cuts at: 806 837 806
Size: 31 4261
Bsp1286I G_dGCh'C
Cuts at: 380 482 505 547 900 982 1094 1113 1306
Size: 102 23 42 353 82 112 19 193
Cuts at: 1306 1917 2010 2084 2274 2336 3134 380
Size: 611 93 74 190 62 798 1538
Fragments arranged by size:
1538 798 611 353 193 190 112 102
93 82 74 62 42 23 19
BspHI T'CATG_A
Cuts at: 2724 2724
Size: 4292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 278 1342 1556 1858 2239 278
Size: 1064 214 302 381 2331
Fragments arranged by size:
2331 1064 381 302 214
BsrBI CCG'CTC
Cuts at: 50 740 2584 2638 4122 4287 50
Size: 690 1844 54 1484 165 55
Fragments arranged by size:
1844 1484 690 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2204 2204
Size: 4292
BsrFI r'CCGG_y
Cuts at: 194 1172 2290 2471 194
Size: 978 1118 181 2015
Fragments arranged by size:
2015 1118 978 181
BssHII G'CGCG_C
Cuts at: 606 606
Size: 4292
BssSI C'ACGA_G
Cuts at: 853 1075 2563 3271 853
Size: 222 1488 708 1874
Fragments arranged by size:
1874 1488 708 222
BstAPI GCAn_nnn'nTGC
Cuts at: 1129 1669 1129
Size: 540 3752
BstBI TT'CG_AA
Cuts at: 2653 2653
Size: 4292
BstXI CCAn_nnnn'nTGG
Cuts at: 264 1176 264
Size: 912 3380
BstYI r'GATC_y
Cuts at: 1 587 1317 1784 1807 2142 2388 2694 2706
Size: 586 730 467 23 335 246 306 12
Cuts at: 2706 2792 3844 1
Size: 86 1052 449
Fragments arranged by size:
1052 730 586 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 154 162 363 548 638 953 107
Size: 47 8 201 185 90 315 3446
Fragments arranged by size:
3446 315 201 185 90 47 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2242 2423 2586 2242
Size: 181 163 3948
Fragments arranged by size:
3948 181 163
BtsI GCAGTG_nn'
Cuts at: 280 1113 1535 280
Size: 833 422 3037
Fragments arranged by size:
3037 833 422
DraI TTT'AAA
Cuts at: 1291 1291
Size: 4292
DraIII CAC_nnn'GTG
Cuts at: 395 639 708 395
Size: 244 69 3979
Fragments arranged by size:
3979 244 69
DrdI GACnn_nn'nnGTC
Cuts at: 1998 3342 1998
Size: 1344 2948
EaeI y'GGCC_r
Cuts at: 165 192 360 431 1877 2051 2442 2469 3814
Size: 27 168 71 1446 174 391 27 1345
Cuts at: 3814 165
Size: 643
Fragments arranged by size:
1446 1345 643 391 174 168 71 27
27
EagI C'GGCC_G
Cuts at: 165 360 1877 165
Size: 195 1517 2580
Fragments arranged by size:
2580 1517 195
EarI CTCTTCn'nnn_
Cuts at: 331 448 829 2315 2525 331
Size: 117 381 1486 210 2098
Fragments arranged by size:
2098 1486 381 210 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2500 3242 3388 4102 2500
Size: 742 146 714 2690
Fragments arranged by size:
2690 742 714 146
EcoICRI GAG'CTC
Cuts at: 980 1304 980
Size: 324 3968
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 403 775 1057 2117 2549 2902 3564 394
Size: 9 372 282 1060 432 353 662 1122
Fragments arranged by size:
1122 1060 662 432 372 353 282 9
EcoO109I rG'GnC_Cy
Cuts at: 1431 1431
Size: 4292
EcoRI G'AATT_C
Cuts at: 1296 1296
Size: 4292
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2311 2311
Size: 4292
FspI TGC'GCA
Cuts at: 2073 2073
Size: 4292
HaeII r_GCGC'y
Cuts at: 162 177 282 338 663 1726 1974 3204 3975
Size: 15 105 56 325 1063 248 1230 771
Cuts at: 3975 162
Size: 479
Fragments arranged by size:
1230 1063 771 479 325 248 105 56
15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 679 711 1261 1293 2134 2164 2166 2196 679
Size: 32 550 32 841 30 2 30 2775
Fragments arranged by size:
2775 841 550 32 32 30 30 2
HincII GTy'rAC
Cuts at: 411 1331 411
Size: 920 3372
KasI G'GCGC_C
Cuts at: 158 278 334 659 1722 1970 158
Size: 120 56 325 1063 248 2480
Fragments arranged by size:
2480 1063 325 248 120 56
KpnI G_GTAC'C
Cuts at: 1312 1312
Size: 4292
MluI A'CGCG_T
Cuts at: 3725 3725
Size: 4292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3051 3235 3051
Size: 184 4108
MscI TGG'CCA
Cuts at: 2053 2053
Size: 4292
MslI CAynn'nnrTG
Cuts at: 262 958 1024 1101 2281 2408 262
Size: 696 66 77 1180 127 2146
Fragments arranged by size:
2146 1180 696 127 77 66
MspA1I CmG'CkG
Cuts at: 156 164 365 774 840 1403 1717 2077 2861
Size: 8 201 409 66 563 314 360 784
Cuts at: 2861 3106 156
Size: 245 1342
Fragments arranged by size:
1342 784 563 409 360 314 245 201
66 8
NaeI GCC'GGC
Cuts at: 1174 2473 1174
Size: 1299 2993
NarI GG'CG_CC
Cuts at: 159 279 335 660 1723 1971 159
Size: 120 56 325 1063 248 2480
Fragments arranged by size:
2480 1063 325 248 120 56
NcoI C'CATG_G
Cuts at: 107 548 107
Size: 441 3851
NgoMIV G'CCGG_C
Cuts at: 1172 2471 1172
Size: 1299 2993
NotI GC'GGCC_GC
Cuts at: 165 165
Size: 4292
NspI r_CATG'y
Cuts at: 579 968 1345 1845 3448 579
Size: 389 377 500 1603 1423
Fragments arranged by size:
1603 1423 500 389 377
PciI A'CATG_T
Cuts at: 3444 3444
Size: 4292
PflMI CCAn_nnn'nTGG
Cuts at: 344 753 344
Size: 409 3883
PmeI GTTT'AAAC
Cuts at: 1291 1291
Size: 4292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 748 780 2738 2770 748
Size: 32 1958 32 2270
Fragments arranged by size:
2270 1958 32 32
PspXI vC'TCGA_Gb
Cuts at: 934 934
Size: 4292
PstI C_TGCA'G
Cuts at: 275 533 1339 2024 275
Size: 258 806 685 2543
Fragments arranged by size:
2543 806 685 258
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4292
PvuII CAG'CTG
Cuts at: 1717 2077 1717
Size: 360 3932
RsrII CG'GwC_CG
Cuts at: 2487 2487
Size: 4292
SacI G_AGCT'C
Cuts at: 982 1306 982
Size: 324 3968
SacII CC_GC'GG
Cuts at: 157 165 366 157
Size: 8 201 4083
Fragments arranged by size:
4083 201 8
SalI G'TCGA_C
Cuts at: 1329 1329
Size: 4292
SapI GCTCTTCn'nnn_
Cuts at: 331 2315 2525 331
Size: 1984 210 2098
Fragments arranged by size:
2098 1984 210
SbfI CC_TGCA'GG
Cuts at: 1339 1339
Size: 4292
ScaI AGT'ACT
Cuts at: 3809 3809
Size: 4292
SexAI A'CCwGG_T
Cuts at: 986 986
Size: 4292
SfcI C'TryA_G
Cuts at: 33 271 529 1335 2020 2988 3179 33
Size: 238 258 806 685 968 191 1146
Fragments arranged by size:
1146 968 806 685 258 238 191
SfoI GGC'GCC
Cuts at: 160 280 336 661 1724 1972 160
Size: 120 56 325 1063 248 2480
Fragments arranged by size:
2480 1063 325 248 120 56
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4292
SmaI CCC'GGG
Cuts at: 138 1314 138
Size: 1176 3116
SmlI C'TyrA_G
Cuts at: 901 934 1112 1447 2362 3076 3338 901
Size: 33 178 335 915 714 262 1855
Fragments arranged by size:
1855 915 714 335 262 178 33
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4292
SphI G_CATG'C
Cuts at: 1345 1845 1345
Size: 500 3792
SrfI GCCC'GGGC
Cuts at: 138 138
Size: 4292
StuI AGG'CCT
Cuts at: 715 715
Size: 4292
StyI C'CwwG_G
Cuts at: 107 548 1280 1426 1780 107
Size: 441 732 146 354 2619
Fragments arranged by size:
2619 732 441 354 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 472 799 1875 2008 2467 2676 472
Size: 327 1076 133 459 209 2088
Fragments arranged by size:
2088 1076 459 327 209 133
TatI w'GTAC_w
Cuts at: 3807 3807
Size: 4292
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 181 213 259 291 379 411 3809 3841 181
Size: 32 46 32 88 32 3398 32 632
Fragments arranged by size:
3398 632 88 46 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2089 2089
Size: 4292
XbaI T'CTAG_A
Cuts at: 1323 1323
Size: 4292
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1787 1787
Size: 4292
XhoI C'TCGA_G
Cuts at: 901 934 1112 901
Size: 33 178 4081
Fragments arranged by size:
4081 178 33
XmaI C'CCGG_G
Cuts at: 136 1312 136
Size: 1176 3116
XmnI GAAnn'nnTTC
Cuts at: 559 559
Size: 4292
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
NspI PciI PflMI PmeI PpiI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI ApaI AscI AvrII
BaeI BcgI BfrBI BmtI BsiWI BsmI BspEI BsrGI
BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NruI NsiI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI
PsrI SanDI SfiI SgrAI SnaBI SspI SwaI ZraI