Restriction map for
pF1KB5139
(Circular) (Six-base) MAPSORT of: pF1KB5139.seq Check: 3662 from: 1 to: 4814
pF1KB5139 4814 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1327 1327
Size: 4814
AccI GT'mk_AC
Cuts at: 803 1852 803
Size: 1049 3765
Acc65I G'GTAC_C
Cuts at: 1146 1830 1146
Size: 684 4130
AclI AA'CG_TT
Cuts at: 4654 4654
Size: 4814
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1578 1662 2639 3071 3818 4086 1578
Size: 84 977 432 747 268 2306
Fragments arranged by size:
2306 977 747 432 268 84
AfeI AGC'GCT
Cuts at: 838 922 1270 838
Size: 84 348 4382
Fragments arranged by size:
4382 348 84
AflIII A'CryG_T
Cuts at: 2166 3270 4247 2166
Size: 1104 977 2733
Fragments arranged by size:
2733 1104 977
AgeI A'CCGG_T
Cuts at: 3202 3202
Size: 4814
AleI CACnn'nnGTG
Cuts at: 238 467 238
Size: 229 4585
AlwNI CAG_nnn'CTG
Cuts at: 198 2119 3686 198
Size: 1921 1567 1326
Fragments arranged by size:
1921 1567 1326
ApaI G_GGCC'C
Cuts at: 133 176 276 519 749 1185 1284 133
Size: 43 100 243 230 436 99 3663
Fragments arranged by size:
3663 436 243 230 100 99 43
ApaLI G'TGCA_C
Cuts at: 700 877 1378 3584 700
Size: 177 501 2206 1930
Fragments arranged by size:
2206 1930 501 177
ApoI r'AATT_y
Cuts at: 1818 4167 4423 1818
Size: 2349 256 2209
Fragments arranged by size:
2349 2209 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4814
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4814
AvaI C'yCGr_G
Cuts at: 907 1086 1834 907
Size: 179 748 3887
Fragments arranged by size:
3887 748 179
BamHI G'GATC_C
Cuts at: 1839 1839
Size: 4814
BanI G'GyrC_C
Cuts at: 253 341 605 1146 1729 1830 2244 2492 2527
Size: 88 264 541 583 101 414 248 35
Cuts at: 2527 253
Size: 2540
Fragments arranged by size:
2540 583 541 414 264 248 101 88
35
BanII G_rGCy'C
Cuts at: 133 176 276 519 594 749 1096 1136 1185
Size: 43 100 243 75 155 347 40 49
Cuts at: 1185 1284 1828 2858 133
Size: 99 544 1030 2089
Fragments arranged by size:
2089 1030 544 347 243 155 100 99
75 49 43 40
BbeI G_GCGC'C
Cuts at: 1733 2248 2496 1733
Size: 515 248 4051
Fragments arranged by size:
4051 515 248
BbsI GAAGACnn'nnnn_
Cuts at: 1357 1357
Size: 4814
BbvCI CC'TCA_GC
Cuts at: 220 710 220
Size: 490 4324
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4780
BciVI GTATCCnnnnn_n'
Cuts at: 2712 3473 4814 2712
Size: 761 1341 2712
Fragments arranged by size:
2712 1341 761
BclI T'GATC_A
Cuts at: 2334 2334
Size: 4814
BglI GCCn_nnn'nGGC
Cuts at: 625 625
Size: 4814
BglII A'GATC_T
Cuts at: 2329 4366 2329
Size: 2037 2777
BlpI GC'TnA_GC
Cuts at: 1926 1926
Size: 4814
Bme1580I G_kGCm'C
Cuts at: 133 176 258 276 288 519 704 749 881
Size: 43 82 18 12 231 185 45 132
Cuts at: 881 907 1185 1284 1382 2439 2532 3588 133
Size: 26 278 99 98 1057 93 1056 1359
Fragments arranged by size:
1359 1057 1056 278 231 185 132 99
98 93 82 45 43 26 18 12
BmgBI CAC'GTC
Cuts at: 236 236
Size: 4814
BmrI ACTGGGnnnn_n'
Cuts at: 240 663 2026 2107 2442 4586 240
Size: 423 1363 81 335 2144 468
Fragments arranged by size:
2144 1363 468 423 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3181 3181
Size: 4814
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 739 1285 739
Size: 546 4268
Bpu10I CC'TnA_GC
Cuts at: 220 710 220
Size: 490 4324
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1990 2869 3361 3659 1990
Size: 879 492 298 3145
Fragments arranged by size:
3145 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4518 4518
Size: 4814
BsaAI yAC'GTr
Cuts at: 705 2167 705
Size: 1462 3352
BsaBI GATnn'nnATC
Cuts at: 6 2328 4365 6
Size: 2322 2037 455
Fragments arranged by size:
2322 2037 455
BsaHI Gr'CG_yC
Cuts at: 1730 2245 2493 3195 4691 1730
Size: 515 248 702 1496 1853
Fragments arranged by size:
1853 1496 702 515 248
BsaWI w'CCGG_w
Cuts at: 2003 2524 3202 3476 3623 4034 2003
Size: 521 678 274 147 411 2783
Fragments arranged by size:
2783 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 785 815 785
Size: 30 4784
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 736 1423 1786 736
Size: 687 363 3764
Fragments arranged by size:
3764 687 363
BseYI C'CCAG_C
Cuts at: 152 311 488 1097 2239 2997 3574 4070 152
Size: 159 177 609 1142 758 577 496 896
Fragments arranged by size:
1142 896 758 609 577 496 177 159
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 397
Size: 4814
BsiEI CG_ry'CG
Cuts at: 104 807 1002 1130 1211 1419 1476 1671 2356
Size: 703 195 128 81 208 57 195 685
Cuts at: 2356 2402 3610 104
Size: 46 1208 1308
Fragments arranged by size:
1308 1208 703 685 208 195 195 128
81 57 46
BsiHKAI G_wGCw'C
Cuts at: 594 704 881 1196 1382 1828 2606 2796 3588
Size: 110 177 315 186 446 778 190 792
Cuts at: 3588 594
Size: 1820
Fragments arranged by size:
1820 792 778 446 315 190 186 177
110
BsmBI CGTCTCn'nnnn_
Cuts at: 555 555
Size: 4814
Bsp1286I G_dGCh'C
Cuts at: 133 176 258 276 288 519 594 704 749
Size: 43 82 18 12 231 75 110 45
Cuts at: 749 881 907 1096 1136 1185 1196 1284 1382
Size: 132 26 189 40 49 11 88 98
Cuts at: 1382 1828 2439 2532 2606 2796 2858 3588 133
Size: 446 611 93 74 190 62 730 1359
Fragments arranged by size:
1359 730 611 446 231 190 189 132
110 98 93 88 82 75 74 62
49 45 43 40 26 18 12 11
BspEI T'CCGG_A
Cuts at: 4034 4034
Size: 4814
BspHI T'CATG_A
Cuts at: 3990 3990
Size: 4814
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1034 1327 1864 2078 2380 2761 1034
Size: 293 537 214 302 381 3087
Fragments arranged by size:
3087 537 381 302 293 214
BsrBI CCG'CTC
Cuts at: 50 891 1308 1392 1644 3106 3160 4644 4809
Size: 841 417 84 252 1462 54 1484 165
Cuts at: 4809 50
Size: 55
Fragments arranged by size:
1484 1462 841 417 252 165 84 55
54
BsrDI GCAATG_nn'
Cuts at: 2726 2726
Size: 4814
BsrFI r'CCGG_y
Cuts at: 338 953 967 1108 1158 1538 1552 1622 2812
Size: 615 14 141 50 380 14 70 1190
Cuts at: 2812 2993 3202 338
Size: 181 209 1950
Fragments arranged by size:
1950 1190 615 380 209 181 141 70
50 14 14
BssSI C'ACGA_G
Cuts at: 3085 3443 3085
Size: 358 4456
BstAPI GCAn_nnn'nTGC
Cuts at: 995 2191 995
Size: 1196 3618
BstBI TT'CG_AA
Cuts at: 3175 3175
Size: 4814
BstEII G'GTnAC_C
Cuts at: 771 771
Size: 4814
BstXI CCAn_nnnn'nTGG
Cuts at: 147 469 741 147
Size: 322 272 4220
Fragments arranged by size:
4220 322 272
BstYI r'GATC_y
Cuts at: 1 1839 2306 2329 2664 2910 3922 4008 4020
Size: 1838 467 23 335 246 1012 86 12
Cuts at: 4020 4366 1
Size: 346 449
Fragments arranged by size:
1838 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 140 213 767 1124 140
Size: 73 554 357 3830
Fragments arranged by size:
3830 554 357 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 262 525 2764 2945 3108 262
Size: 263 2239 181 163 1968
Fragments arranged by size:
2239 1968 263 181 163
BtsI GCAGTG_nn'
Cuts at: 409 2057 409
Size: 1648 3166
DraI TTT'AAA
Cuts at: 1813 1813
Size: 4814
DraIII CAC_nnn'GTG
Cuts at: 1362 1362
Size: 4814
DrdI GACnn_nn'nnGTC
Cuts at: 2520 3378 2520
Size: 858 3956
EaeI y'GGCC_r
Cuts at: 137 378 654 999 1127 1416 1752 2399 2573
Size: 241 276 345 128 289 336 647 174
Cuts at: 2573 2964 2991 4336 137
Size: 391 27 1345 615
Fragments arranged by size:
1345 647 615 391 345 336 289 276
241 174 128 27
EagI C'GGCC_G
Cuts at: 999 1127 1416 2399 999
Size: 128 289 983 3414
Fragments arranged by size:
3414 983 289 128
EarI CTCTTCn'nnn_
Cuts at: 2837 3047 2837
Size: 210 4604
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1501 3022 3332 3478 4624 1501
Size: 1521 310 146 1146 1691
Fragments arranged by size:
1691 1521 1146 310 146
EcoICRI GAG'CTC
Cuts at: 592 1826 592
Size: 1234 3580
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 739 1285 1578 1662 2639 3071 3818 4086 739
Size: 546 293 84 977 432 747 268 1467
Fragments arranged by size:
1467 977 747 546 432 293 268 84
EcoNI CCTnn'n_nnAGG
Cuts at: 202 721 202
Size: 519 4295
EcoO109I rG'GnC_Cy
Cuts at: 129 130 173 272 714 745 746 1181 1182
Size: 1 43 99 442 31 1 435 1
Cuts at: 1182 1203 1953 129
Size: 21 750 2990
Fragments arranged by size:
2990 750 442 435 99 43 31 21
1 1 1
EcoRI G'AATT_C
Cuts at: 1818 1818
Size: 4814
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 873 2833 873
Size: 1960 2854
FspI TGC'GCA
Cuts at: 2595 2595
Size: 4814
HaeII r_GCGC'y
Cuts at: 840 924 1272 1733 2248 2496 3518 4497 840
Size: 84 348 461 515 248 1022 979 1157
Fragments arranged by size:
1157 1022 979 515 461 348 248 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 785 817 2656 2686 2688 2718 785
Size: 32 1839 30 2 30 2881
Fragments arranged by size:
2881 1839 32 30 30 2
HincII GTy'rAC
Cuts at: 804 1853 804
Size: 1049 3765
KasI G'GCGC_C
Cuts at: 1729 2244 2492 1729
Size: 515 248 4051
Fragments arranged by size:
4051 515 248
KpnI G_GTAC'C
Cuts at: 1150 1834 1150
Size: 684 4130
MluI A'CGCG_T
Cuts at: 4247 4247
Size: 4814
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1631 1715 3485 3669 1631
Size: 84 1770 184 2776
Fragments arranged by size:
2776 1770 184 84
MscI TGG'CCA
Cuts at: 139 1754 2575 139
Size: 1615 821 2378
Fragments arranged by size:
2378 1615 821
MslI CAynn'nnrTG
Cuts at: 238 281 467 2803 2930 238
Size: 43 186 2336 127 2122
Fragments arranged by size:
2336 2122 186 127 43
MspA1I CmG'CkG
Cuts at: 215 526 769 1101 1126 1235 1290 1925 2239
Size: 311 243 332 25 109 55 635 314
Cuts at: 2239 2599 3612 3857 215
Size: 360 1013 245 1172
Fragments arranged by size:
1172 1013 635 360 332 314 311 245
243 109 55 25
NaeI GCC'GGC
Cuts at: 1110 2995 1110
Size: 1885 2929
NarI GG'CG_CC
Cuts at: 1730 2245 2493 1730
Size: 515 248 4051
Fragments arranged by size:
4051 515 248
NcoI C'CATG_G
Cuts at: 140 140
Size: 4814
NdeI CA'TA_TG
Cuts at: 640 640
Size: 4814
NgoMIV G'CCGG_C
Cuts at: 1108 2993 1108
Size: 1885 2929
NotI GC'GGCC_GC
Cuts at: 999 1416 999
Size: 417 4397
NspI r_CATG'y
Cuts at: 1867 2367 3274 1867
Size: 500 907 3407
Fragments arranged by size:
3407 907 500
PasI CC'CwG_GG
Cuts at: 198 755 1431 198
Size: 557 676 3581
Fragments arranged by size:
3581 676 557
PciI A'CATG_T
Cuts at: 3270 3270
Size: 4814
PflMI CCAn_nnn'nTGG
Cuts at: 198 198
Size: 4814
PfoI T'CCnGG_A
Cuts at: 1721 1721
Size: 4814
PmeI GTTT'AAAC
Cuts at: 1813 1813
Size: 4814
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3953 3985 3953
Size: 32 4782
PpuMI rG'GwC_Cy
Cuts at: 1203 1203
Size: 4814
PspOMI G'GGCC_C
Cuts at: 129 172 272 515 745 1181 1280 129
Size: 43 100 243 230 436 99 3663
Fragments arranged by size:
3663 436 243 230 100 99 43
PstI C_TGCA'G
Cuts at: 1861 2546 1861
Size: 685 4129
PvuI CG_AT'CG
Cuts at: 104 1211 1476 104
Size: 1107 265 3442
Fragments arranged by size:
3442 1107 265
PvuII CAG'CTG
Cuts at: 526 1101 1235 2239 2599 526
Size: 575 134 1004 360 2741
Fragments arranged by size:
2741 1004 575 360 134
RsrII CG'GwC_CG
Cuts at: 3009 3009
Size: 4814
SacI G_AGCT'C
Cuts at: 594 1828 594
Size: 1234 3580
SacII CC_GC'GG
Cuts at: 216 770 1127 216
Size: 554 357 3903
Fragments arranged by size:
3903 554 357
SalI G'TCGA_C
Cuts at: 802 1851 802
Size: 1049 3765
SapI GCTCTTCn'nnn_
Cuts at: 2837 3047 2837
Size: 210 4604
SbfI CC_TGCA'GG
Cuts at: 1861 1861
Size: 4814
ScaI AGT'ACT
Cuts at: 4331 4331
Size: 4814
SexAI A'CCwGG_T
Cuts at: 1358 1610 1358
Size: 252 4562
SfcI C'TryA_G
Cuts at: 33 1230 1857 2542 3535 3726 33
Size: 1197 627 685 993 191 1121
Fragments arranged by size:
1197 1121 993 685 627 191
SfoI GGC'GCC
Cuts at: 1731 2246 2494 1731
Size: 515 248 4051
Fragments arranged by size:
4051 515 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4814
SgrAI Cr'CCGG_yG
Cuts at: 338 953 967 1538 1552 1622 338
Size: 615 14 571 14 70 3530
Fragments arranged by size:
3530 615 571 70 14 14
SmaI CCC'GGG
Cuts at: 1088 1836 1088
Size: 748 4066
SmlI C'TyrA_G
Cuts at: 1969 2884 3376 3638 1969
Size: 915 492 262 3145
Fragments arranged by size:
3145 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4814
SphI G_CATG'C
Cuts at: 1867 2367 1867
Size: 500 4314
StuI AGG'CCT
Cuts at: 1507 1507
Size: 4814
StyI C'CwwG_G
Cuts at: 140 734 1948 2302 140
Size: 594 1214 354 2652
Fragments arranged by size:
2652 1214 594 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 303 480 1211 1717 2397 2530 2989 3198 303
Size: 177 731 506 680 133 459 209 1919
Fragments arranged by size:
1919 731 680 506 459 209 177 133
TatI w'GTAC_w
Cuts at: 4329 4329
Size: 4814
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1039 1071 4331 4363 1039
Size: 32 3260 32 1490
Fragments arranged by size:
3260 1490 32 32
Tth111I GACn'n_nGTC
Cuts at: 2611 2611
Size: 4814
XbaI T'CTAG_A
Cuts at: 1845 1845
Size: 4814
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 444 2309 444
Size: 1865 2949
XmaI C'CCGG_G
Cuts at: 1086 1834 1086
Size: 748 4066
XmnI GAAnn'nnTTC
Cuts at: 1339 1339
Size: 4814
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BaeI
BfrBI BmtI BsiWI BsmI BsrGI BssHII BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NheI NruI NsiI PacI PmlI PshAI PsiI PspXI
PsrI SanDI SfiI SnaBI SrfI SspI SwaI XhoI
ZraI