Restriction map for
pF1KB5138
(Circular) (Six-base) MAPSORT of: pF1KB5138.seq Check: 3838 from: 1 to: 5498
pF1KB5138 5498 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 2536 2536
Size: 5498
Acc65I G'GTAC_C
Cuts at: 2514 2514
Size: 5498
AclI AA'CG_TT
Cuts at: 5338 5338
Size: 5498
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 2341 2398 3323 3755 4108 4770 706
Size: 1635 57 925 432 353 662 1434
Fragments arranged by size:
1635 1434 925 662 432 353 57
AflIII A'CryG_T
Cuts at: 1290 2850 4650 4931 1290
Size: 1560 1800 281 1857
Fragments arranged by size:
1857 1800 1560 281
AleI CACnn'nnGTG
Cuts at: 670 1216 670
Size: 546 4952
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1843 1843
Size: 5498
AlwNI CAG_nnn'CTG
Cuts at: 1068 1086 2235 2803 4241 1068
Size: 18 1149 568 1438 2325
Fragments arranged by size:
2325 1438 1149 568 18
ApaLI G'TGCA_C
Cuts at: 4336 4336
Size: 5498
ApoI r'AATT_y
Cuts at: 397 608 811 1398 2153 2502 4851 5107 397
Size: 211 203 587 755 349 2349 256 788
Fragments arranged by size:
2349 788 755 587 349 256 211 203
AseI AT'TA_AT
Cuts at: 20 1589 2474 20
Size: 1569 885 3044
Fragments arranged by size:
3044 1569 885
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5498
AvaI C'yCGr_G
Cuts at: 285 2518 285
Size: 2233 3265
BamHI G'GATC_C
Cuts at: 2523 2523
Size: 5498
BanI G'GyrC_C
Cuts at: 895 2025 2514 2928 3176 3211 895
Size: 1130 489 414 248 35 3182
Fragments arranged by size:
3182 1130 489 414 248 35
BanII G_rGCy'C
Cuts at: 223 1028 1891 2512 3542 223
Size: 805 863 621 1030 2179
Fragments arranged by size:
2179 1030 863 805 621
BbeI G_GCGC'C
Cuts at: 899 2932 3180 899
Size: 2033 248 3217
Fragments arranged by size:
3217 2033 248
BbsI GAAGACnn'nnnn_
Cuts at: 644 847 1214 1771 2140 644
Size: 203 367 557 369 4002
Fragments arranged by size:
4002 557 369 367 203
BbvCI CC'TCA_GC
Cuts at: 1784 1979 1784
Size: 195 5303
BciVI GTATCCnnnnn_n'
Cuts at: 3396 4452 5498 3396
Size: 1056 1046 3396
Fragments arranged by size:
3396 1056 1046
BclI T'GATC_A
Cuts at: 3018 3018
Size: 5498
BglII A'GATC_T
Cuts at: 1171 3013 5050 1171
Size: 1842 2037 1619
Fragments arranged by size:
2037 1842 1619
BlpI GC'TnA_GC
Cuts at: 1079 2610 1079
Size: 1531 3967
Bme1580I G_kGCm'C
Cuts at: 736 3123 3216 4340 736
Size: 2387 93 1124 1894
Fragments arranged by size:
2387 1894 1124 93
BmgBI CAC'GTC
Cuts at: 2199 2199
Size: 5498
BmrI ACTGGGnnnn_n'
Cuts at: 1359 2710 2791 3126 5270 1359
Size: 1351 81 335 2144 1587
Fragments arranged by size:
2144 1587 1351 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 698 1770 1910 3865 698
Size: 1072 140 1955 2331
Fragments arranged by size:
2331 1955 1072 140
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 1709 2087 2164 226
Size: 1483 378 77 3560
Fragments arranged by size:
3560 1483 378 77
Bpu10I CC'TnA_GC
Cuts at: 1784 1979 1784
Size: 195 5303
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1628 2287 2674 3553 4267 4565 1628
Size: 659 387 879 714 298 2561
Fragments arranged by size:
2561 879 714 659 387 298
BsaI GGTCTCn'nnnn_
Cuts at: 1421 5202 1421
Size: 3781 1717
BsaAI yAC'GTr
Cuts at: 540 1291 2851 540
Size: 751 1560 3187
Fragments arranged by size:
3187 1560 751
BsaBI GATnn'nnATC
Cuts at: 6 587 707 3012 5049 6
Size: 581 120 2305 2037 455
Fragments arranged by size:
2305 2037 581 455 120
BsaHI Gr'CG_yC
Cuts at: 896 2929 3177 3879 5375 896
Size: 2033 248 702 1496 1019
Fragments arranged by size:
2033 1496 1019 702 248
BsaWI w'CCGG_w
Cuts at: 995 1413 2332 2687 3208 3886 4297 4444 4718
Size: 418 919 355 521 678 411 147 274
Cuts at: 4718 995
Size: 1775
Fragments arranged by size:
1775 919 678 521 418 411 355 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 237 267 237
Size: 30 5468
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 400 1967 2383 400
Size: 1567 416 3515
Fragments arranged by size:
3515 1567 416
BseYI C'CCAG_C
Cuts at: 887 1121 1510 2243 2923 3681 4346 4754 887
Size: 234 389 733 680 758 665 408 1631
Fragments arranged by size:
1631 758 733 680 665 408 389 234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1000 1940 1000
Size: 940 4558
BsiEI CG_ry'CG
Cuts at: 104 3040 3086 4316 104
Size: 2936 46 1230 1286
Fragments arranged by size:
2936 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2512 3290 3480 4340 2512
Size: 778 190 860 3670
Fragments arranged by size:
3670 860 778 190
BsmI GAATG_Cn'
Cuts at: 684 1470 684
Size: 786 4712
Bsp1286I G_dGCh'C
Cuts at: 223 736 1028 1891 2512 3123 3216 3290 3480
Size: 513 292 863 621 611 93 74 190
Cuts at: 3480 3542 4340 223
Size: 62 798 1381
Fragments arranged by size:
1381 863 798 621 611 513 292 190
93 74 62
BspEI T'CCGG_A
Cuts at: 995 995
Size: 5498
BspHI T'CATG_A
Cuts at: 3930 3930
Size: 5498
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1022 2548 2762 3064 3445 1022
Size: 1526 214 302 381 3075
Fragments arranged by size:
3075 1526 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3790 3844 5328 5493 50
Size: 3740 54 1484 165 55
Fragments arranged by size:
3740 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 448 3410 448
Size: 2962 2536
BsrFI r'CCGG_y
Cuts at: 3496 3677 3496
Size: 181 5317
BssSI C'ACGA_G
Cuts at: 3769 4477 3769
Size: 708 4790
BstAPI GCAn_nnn'nTGC
Cuts at: 270 1654 1663 2875 270
Size: 1384 9 1212 2893
Fragments arranged by size:
2893 1384 1212 9
BstBI TT'CG_AA
Cuts at: 3859 3859
Size: 5498
BstYI r'GATC_y
Cuts at: 1 1171 1716 2523 2990 3013 3348 3594 3900
Size: 1170 545 807 467 23 335 246 306
Cuts at: 3900 3912 3998 5050 1
Size: 12 86 1052 449
Fragments arranged by size:
1170 1052 807 545 467 449 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 310 310
Size: 5498
BtgI C'CryG_G
Cuts at: 107 975 1478 1836 1960 2080 2090 2310 107
Size: 868 503 358 124 120 10 220 3295
Fragments arranged by size:
3295 868 503 358 220 124 120 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1262 3448 3629 3792 1262
Size: 2186 181 163 2968
Fragments arranged by size:
2968 2186 181 163
BtsI GCAGTG_nn'
Cuts at: 1109 2741 1109
Size: 1632 3866
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1887 1922 1887
Size: 35 5463
DraI TTT'AAA
Cuts at: 278 2497 278
Size: 2219 3279
DrdI GACnn_nn'nnGTC
Cuts at: 3204 4548 3204
Size: 1344 4154
EaeI y'GGCC_r
Cuts at: 722 915 1475 2140 3083 3257 3648 3675 5020
Size: 193 560 665 943 174 391 27 1345
Cuts at: 5020 722
Size: 1200
Fragments arranged by size:
1345 1200 943 665 560 391 193 174
27
EagI C'GGCC_G
Cuts at: 3083 3083
Size: 5498
EarI CTCTTCn'nnn_
Cuts at: 225 1010 1337 2213 2360 2384 2438 3521 3731
Size: 785 327 876 147 24 54 1083 210
Cuts at: 3731 225
Size: 1992
Fragments arranged by size:
1992 1083 876 785 327 210 147 54
24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3706 4448 4594 5308 3706
Size: 742 146 714 3896
Fragments arranged by size:
3896 742 714 146
EcoICRI GAG'CTC
Cuts at: 2510 2510
Size: 5498
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 706 1709 2087 2164 2341 2398 3323 3755
Size: 480 1003 378 77 177 57 925 432
Cuts at: 3755 4108 4770 226
Size: 353 662 954
Fragments arranged by size:
1003 954 925 662 480 432 378 353
177 77 57
EcoNI CCTnn'n_nnAGG
Cuts at: 1336 1336
Size: 5498
EcoO109I rG'GnC_Cy
Cuts at: 1347 1483 2065 2637 1347
Size: 136 582 572 4208
Fragments arranged by size:
4208 582 572 136
EcoRI G'AATT_C
Cuts at: 2502 2502
Size: 5498
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3517 3517
Size: 5498
FspI TGC'GCA
Cuts at: 965 3279 965
Size: 2314 3184
HaeII r_GCGC'y
Cuts at: 899 2932 3180 4410 5181 899
Size: 2033 248 1230 771 1216
Fragments arranged by size:
2033 1230 1216 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1253 1285 2052 2084 3340 3370 3372 3402 1253
Size: 32 767 32 1256 30 2 30 3349
Fragments arranged by size:
3349 1256 767 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1059 2537 1059
Size: 1478 4020
KasI G'GCGC_C
Cuts at: 895 2928 3176 895
Size: 2033 248 3217
Fragments arranged by size:
3217 2033 248
KpnI G_GTAC'C
Cuts at: 2518 2518
Size: 5498
MfeI C'AATT_G
Cuts at: 1051 1051
Size: 5498
MluI A'CGCG_T
Cuts at: 4931 4931
Size: 5498
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 489 4257 4441 489
Size: 3768 184 1546
Fragments arranged by size:
3768 1546 184
MscI TGG'CCA
Cuts at: 724 917 3259 724
Size: 193 2342 2963
Fragments arranged by size:
2963 2342 193
MslI CAynn'nnrTG
Cuts at: 670 1216 1835 3487 3614 670
Size: 546 619 1652 127 2554
Fragments arranged by size:
2554 1652 619 546 127
MspA1I CmG'CkG
Cuts at: 435 1068 1083 2292 2609 2923 3283 4067 4312
Size: 633 15 1209 317 314 360 784 245
Cuts at: 4312 435
Size: 1621
Fragments arranged by size:
1621 1209 784 633 360 317 314 245
15
NaeI GCC'GGC
Cuts at: 3679 3679
Size: 5498
NarI GG'CG_CC
Cuts at: 896 2929 3177 896
Size: 2033 248 3217
Fragments arranged by size:
3217 2033 248
NcoI C'CATG_G
Cuts at: 107 975 1478 1836 1960 107
Size: 868 503 358 124 3645
Fragments arranged by size:
3645 868 503 358 124
NgoMIV G'CCGG_C
Cuts at: 3677 3677
Size: 5498
NspI r_CATG'y
Cuts at: 394 2551 3051 4654 394
Size: 2157 500 1603 1238
Fragments arranged by size:
2157 1603 1238 500
PasI CC'CwG_GG
Cuts at: 1021 1021
Size: 5498
PciI A'CATG_T
Cuts at: 4650 4650
Size: 5498
PflMI CCAn_nnn'nTGG
Cuts at: 1068 1068
Size: 5498
PmeI GTTT'AAAC
Cuts at: 2497 2497
Size: 5498
PmlI CAC'GTG
Cuts at: 1291 1291
Size: 5498
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3944 3976 3944
Size: 32 5466
PpuMI rG'GwC_Cy
Cuts at: 1347 1483 1347
Size: 136 5362
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 760 792 2278 2310 760
Size: 32 1486 32 3948
Fragments arranged by size:
3948 1486 32 32
PstI C_TGCA'G
Cuts at: 2545 3230 2545
Size: 685 4813
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5498
PvuII CAG'CTG
Cuts at: 435 1068 1083 2923 3283 435
Size: 633 15 1840 360 2650
Fragments arranged by size:
2650 1840 633 360 15
RsrII CG'GwC_CG
Cuts at: 3693 3693
Size: 5498
SacI G_AGCT'C
Cuts at: 2512 2512
Size: 5498
SalI G'TCGA_C
Cuts at: 2535 2535
Size: 5498
SapI GCTCTTCn'nnn_
Cuts at: 2438 3521 3731 2438
Size: 1083 210 4205
Fragments arranged by size:
4205 1083 210
SbfI CC_TGCA'GG
Cuts at: 2545 2545
Size: 5498
ScaI AGT'ACT
Cuts at: 774 5015 774
Size: 4241 1257
SfcI C'TryA_G
Cuts at: 33 560 2541 3226 4194 4385 33
Size: 527 1981 685 968 191 1146
Fragments arranged by size:
1981 1146 968 685 527 191
SfoI GGC'GCC
Cuts at: 897 2930 3178 897
Size: 2033 248 3217
Fragments arranged by size:
3217 2033 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5498
SmaI CCC'GGG
Cuts at: 2520 2520
Size: 5498
SmlI C'TyrA_G
Cuts at: 1643 2302 2653 3568 4282 4544 1643
Size: 659 351 915 714 262 2597
Fragments arranged by size:
2597 915 714 659 351 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5498
SphI G_CATG'C
Cuts at: 394 2551 3051 394
Size: 2157 500 2841
Fragments arranged by size:
2841 2157 500
StuI AGG'CCT
Cuts at: 1943 1943
Size: 5498
StyI C'CwwG_G
Cuts at: 107 725 975 1478 1836 1905 1960 2168 2632
Size: 618 250 503 358 69 55 208 464
Cuts at: 2632 2986 107
Size: 354 2619
Fragments arranged by size:
2619 618 503 464 358 354 250 208
69 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1445 1524 3081 3214 3673 3882 1445
Size: 79 1557 133 459 209 3061
Fragments arranged by size:
3061 1557 459 209 133 79
TatI w'GTAC_w
Cuts at: 247 772 2261 5013 247
Size: 525 1489 2752 732
Fragments arranged by size:
2752 1489 732 525
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 237 269 5015 5047 237
Size: 32 4746 32 688
Fragments arranged by size:
4746 688 32 32
Tth111I GACn'n_nGTC
Cuts at: 2235 3295 2235
Size: 1060 4438
XbaI T'CTAG_A
Cuts at: 1921 2529 1921
Size: 608 4890
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2993 2993
Size: 5498
XmaI C'CCGG_G
Cuts at: 2518 2518
Size: 5498
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmtI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI DraIII
EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI
NruI NsiI PacI PfoI PshAI PsiI PspOMI PspXI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI