Restriction map for
pF1KB5136
(Circular) (Six-base) MAPSORT of: pF1KB5136.seq Check: 1594 from: 1 to: 3911
pF1KB5136 3911 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 688 688
Size: 3911
AccI GT'mk_AC
Cuts at: 949 949
Size: 3911
Acc65I G'GTAC_C
Cuts at: 927 927
Size: 3911
AclI AA'CG_TT
Cuts at: 753 3751 753
Size: 2998 913
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 1736 2168 2915 3183 376
Size: 1360 432 747 268 1104
Fragments arranged by size:
1360 1104 747 432 268
AflIII A'CryG_T
Cuts at: 1263 2367 3344 1263
Size: 1104 977 1830
Fragments arranged by size:
1830 1104 977
AgeI A'CCGG_T
Cuts at: 2299 2299
Size: 3911
AlwNI CAG_nnn'CTG
Cuts at: 689 1216 2783 689
Size: 527 1567 1817
Fragments arranged by size:
1817 1567 527
ApaLI G'TGCA_C
Cuts at: 2681 2681
Size: 3911
ApoI r'AATT_y
Cuts at: 915 3264 3520 915
Size: 2349 256 1306
Fragments arranged by size:
2349 1306 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3911
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3911
AvaI C'yCGr_G
Cuts at: 931 931
Size: 3911
BamHI G'GATC_C
Cuts at: 936 936
Size: 3911
BanI G'GyrC_C
Cuts at: 927 1341 1589 1624 927
Size: 414 248 35 3214
Fragments arranged by size:
3214 414 248 35
BanII G_rGCy'C
Cuts at: 925 1955 925
Size: 1030 2881
BbeI G_GCGC'C
Cuts at: 1345 1593 1345
Size: 248 3663
BbsI GAAGACnn'nnnn_
Cuts at: 431 431
Size: 3911
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3877
BciVI GTATCCnnnnn_n'
Cuts at: 1809 2570 3911 1809
Size: 761 1341 1809
Fragments arranged by size:
1809 1341 761
BclI T'GATC_A
Cuts at: 1431 1431
Size: 3911
BglII A'GATC_T
Cuts at: 1426 3463 1426
Size: 2037 1874
BlpI GC'TnA_GC
Cuts at: 1023 1023
Size: 3911
Bme1580I G_kGCm'C
Cuts at: 425 1536 1629 2685 425
Size: 1111 93 1056 1651
Fragments arranged by size:
1651 1111 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 403 422 1123 1204 1539 3683 403
Size: 19 701 81 335 2144 631
Fragments arranged by size:
2144 701 631 335 81 19
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2278 2278
Size: 3911
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1087 1966 2458 2756 1087
Size: 879 492 298 2242
Fragments arranged by size:
2242 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 734 3615 734
Size: 2881 1030
BsaAI yAC'GTr
Cuts at: 796 1264 796
Size: 468 3443
BsaBI GATnn'nnATC
Cuts at: 6 1425 3462 6
Size: 1419 2037 455
Fragments arranged by size:
2037 1419 455
BsaHI Gr'CG_yC
Cuts at: 1342 1590 2292 3788 1342
Size: 248 702 1496 1465
Fragments arranged by size:
1496 1465 702 248
BsaWI w'CCGG_w
Cuts at: 1100 1621 2299 2573 2720 3131 1100
Size: 521 678 274 147 411 1880
Fragments arranged by size:
1880 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 686 686
Size: 3911
BseYI C'CCAG_C
Cuts at: 291 1336 2094 2671 3167 291
Size: 1045 758 577 496 1035
Fragments arranged by size:
1045 1035 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 358
Size: 3911
BsiEI CG_ry'CG
Cuts at: 104 1453 1499 2707 104
Size: 1349 46 1208 1308
Fragments arranged by size:
1349 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 615 925 1703 1893 2685 615
Size: 310 778 190 792 1841
Fragments arranged by size:
1841 792 778 310 190
Bsp1286I G_dGCh'C
Cuts at: 425 615 925 1536 1629 1703 1893 1955 2685
Size: 190 310 611 93 74 190 62 730
Cuts at: 2685 425
Size: 1651
Fragments arranged by size:
1651 730 611 310 190 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3131 3131
Size: 3911
BspHI T'CATG_A
Cuts at: 3087 3087
Size: 3911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 688 961 1175 1477 1858 688
Size: 273 214 302 381 2741
Fragments arranged by size:
2741 381 302 273 214
BsrBI CCG'CTC
Cuts at: 50 2203 2257 3741 3906 50
Size: 2153 54 1484 165 55
Fragments arranged by size:
2153 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1823 1823
Size: 3911
BsrFI r'CCGG_y
Cuts at: 1909 2090 2299 1909
Size: 181 209 3521
Fragments arranged by size:
3521 209 181
BssSI C'ACGA_G
Cuts at: 2182 2540 2182
Size: 358 3553
BstAPI GCAn_nnn'nTGC
Cuts at: 689 1288 689
Size: 599 3312
BstBI TT'CG_AA
Cuts at: 222 2272 222
Size: 2050 1861
BstYI r'GATC_y
Cuts at: 1 936 1403 1426 1761 2007 3019 3105 3117
Size: 935 467 23 335 246 1012 86 12
Cuts at: 3117 3463 1
Size: 346 449
Fragments arranged by size:
1012 935 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1861 2042 2205 1861
Size: 181 163 3567
Fragments arranged by size:
3567 181 163
BtsI GCAGTG_nn'
Cuts at: 690 1154 690
Size: 464 3447
DraI TTT'AAA
Cuts at: 910 910
Size: 3911
DrdI GACnn_nn'nnGTC
Cuts at: 1617 2475 1617
Size: 858 3053
EaeI y'GGCC_r
Cuts at: 513 658 1496 1670 2061 2088 3433 513
Size: 145 838 174 391 27 1345 991
Fragments arranged by size:
1345 991 838 391 174 145 27
EagI C'GGCC_G
Cuts at: 1496 1496
Size: 3911
EarI CTCTTCn'nnn_
Cuts at: 1934 2144 1934
Size: 210 3701
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 432 2119 2429 2575 3721 432
Size: 1687 310 146 1146 622
Fragments arranged by size:
1687 1146 622 310 146
EcoICRI GAG'CTC
Cuts at: 923 923
Size: 3911
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 1736 2168 2915 3183 376
Size: 1360 432 747 268 1104
Fragments arranged by size:
1360 1104 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 288 1050 288
Size: 762 3149
EcoRI G'AATT_C
Cuts at: 915 915
Size: 3911
EcoRV GAT'ATC
Cuts at: 189 189
Size: 3911
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1930 1930
Size: 3911
FspI TGC'GCA
Cuts at: 561 1692 561
Size: 1131 2780
HaeII r_GCGC'y
Cuts at: 1345 1593 2615 3594 1345
Size: 248 1022 979 1662
Fragments arranged by size:
1662 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 389 421 731 763 1753 1783 1785 1815 389
Size: 32 310 32 990 30 2 30 2485
Fragments arranged by size:
2485 990 310 32 32 30 30 2
HincII GTy'rAC
Cuts at: 729 950 729
Size: 221 3690
HpaI GTT'AAC
Cuts at: 729 729
Size: 3911
KasI G'GCGC_C
Cuts at: 1341 1589 1341
Size: 248 3663
KpnI G_GTAC'C
Cuts at: 931 931
Size: 3911
MfeI C'AATT_G
Cuts at: 327 327
Size: 3911
MluI A'CGCG_T
Cuts at: 3344 3344
Size: 3911
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2582 2766 2582
Size: 184 3727
MscI TGG'CCA
Cuts at: 515 660 1672 515
Size: 145 1012 2754
Fragments arranged by size:
2754 1012 145
MslI CAynn'nnrTG
Cuts at: 1900 2027 1900
Size: 127 3784
MspA1I CmG'CkG
Cuts at: 1022 1336 1696 2709 2954 1022
Size: 314 360 1013 245 1979
Fragments arranged by size:
1979 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2092 2092
Size: 3911
NarI GG'CG_CC
Cuts at: 1342 1590 1342
Size: 248 3663
NgoMIV G'CCGG_C
Cuts at: 2090 2090
Size: 3911
NspI r_CATG'y
Cuts at: 964 1464 2371 964
Size: 500 907 2504
Fragments arranged by size:
2504 907 500
PciI A'CATG_T
Cuts at: 2367 2367
Size: 3911
PfoI T'CCnGG_A
Cuts at: 470 470
Size: 3911
PmeI GTTT'AAAC
Cuts at: 910 910
Size: 3911
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3050 3082 3050
Size: 32 3879
PpuMI rG'GwC_Cy
Cuts at: 288 288
Size: 3911
PsiI TTA'TAA
Cuts at: 856 856
Size: 3911
PstI C_TGCA'G
Cuts at: 685 958 1643 685
Size: 273 685 2953
Fragments arranged by size:
2953 685 273
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3911
PvuII CAG'CTG
Cuts at: 1336 1696 1336
Size: 360 3551
RsrII CG'GwC_CG
Cuts at: 2106 2106
Size: 3911
SacI G_AGCT'C
Cuts at: 925 925
Size: 3911
SalI G'TCGA_C
Cuts at: 948 948
Size: 3911
SanDI GG'GwC_CC
Cuts at: 288 288
Size: 3911
SapI GCTCTTCn'nnn_
Cuts at: 1934 2144 1934
Size: 210 3701
SbfI CC_TGCA'GG
Cuts at: 958 958
Size: 3911
ScaI AGT'ACT
Cuts at: 3428 3428
Size: 3911
SfcI C'TryA_G
Cuts at: 33 681 954 1639 2632 2823 33
Size: 648 273 685 993 191 1121
Fragments arranged by size:
1121 993 685 648 273 191
SfoI GGC'GCC
Cuts at: 1343 1591 1343
Size: 248 3663
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3911
SmaI CCC'GGG
Cuts at: 933 933
Size: 3911
SmlI C'TyrA_G
Cuts at: 1066 1981 2473 2735 1066
Size: 915 492 262 2242
Fragments arranged by size:
2242 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3911
SphI G_CATG'C
Cuts at: 964 1464 964
Size: 500 3411
SspI AAT'ATT
Cuts at: 177 177
Size: 3911
StyI C'CwwG_G
Cuts at: 1045 1399 1045
Size: 354 3557
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 392 1494 1627 2086 2295 392
Size: 1102 133 459 209 2008
Fragments arranged by size:
2008 1102 459 209 133
TatI w'GTAC_w
Cuts at: 3426 3426
Size: 3911
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3428 3460 3428
Size: 32 3879
Tth111I GACn'n_nGTC
Cuts at: 414 745 1708 414
Size: 331 963 2617
Fragments arranged by size:
2617 963 331
XbaI T'CTAG_A
Cuts at: 942 942
Size: 3911
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1406 1406
Size: 3911
XmaI C'CCGG_G
Cuts at: 931 931
Size: 3911
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI FseI FspAI HindIII NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI ZraI