Restriction map for
pF1KB5134
(Circular) (Six-base) MAPSORT of: pF1KB5134.seq Check: 8180 from: 1 to: 5501
pF1KB5134 5501 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 206 234 206
Size: 28 5473
AccI GT'mk_AC
Cuts at: 923 2539 923
Size: 1616 3885
Acc65I G'GTAC_C
Cuts at: 2517 2517
Size: 5501
AclI AA'CG_TT
Cuts at: 5341 5341
Size: 5501
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 641 1677 3326 3758 4111 4773 641
Size: 1036 1649 432 353 662 1369
Fragments arranged by size:
1649 1369 1036 662 432 353
AfeI AGC'GCT
Cuts at: 256 256
Size: 5501
AflIII A'CryG_T
Cuts at: 479 2853 4653 4934 479
Size: 2374 1800 281 1046
Fragments arranged by size:
2374 1800 1046 281
AlwNI CAG_nnn'CTG
Cuts at: 1300 2806 4244 1300
Size: 1506 1438 2557
Fragments arranged by size:
2557 1506 1438
ApaLI G'TGCA_C
Cuts at: 1162 4339 1162
Size: 3177 2324
ApoI r'AATT_y
Cuts at: 538 1725 2505 4854 5110 538
Size: 1187 780 2349 256 929
Fragments arranged by size:
2349 1187 929 780 256
AseI AT'TA_AT
Cuts at: 20 563 1406 20
Size: 543 843 4115
Fragments arranged by size:
4115 843 543
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5501
AvaI C'yCGr_G
Cuts at: 2521 2521
Size: 5501
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2188 2221 2188
Size: 33 5468
BamHI G'GATC_C
Cuts at: 2526 2526
Size: 5501
BanI G'GyrC_C
Cuts at: 194 218 1303 1960 2517 2931 3179 3214 194
Size: 24 1085 657 557 414 248 35 2481
Fragments arranged by size:
2481 1085 657 557 414 248 35 24
BanII G_rGCy'C
Cuts at: 474 1247 2231 2356 2377 2515 3545 474
Size: 773 984 125 21 138 1030 2430
Fragments arranged by size:
2430 1030 984 773 138 125 21
BbeI G_GCGC'C
Cuts at: 1964 2935 3183 1964
Size: 971 248 4282
Fragments arranged by size:
4282 971 248
BbsI GAAGACnn'nnnn_
Cuts at: 2126 2126
Size: 5501
BciVI GTATCCnnnnn_n'
Cuts at: 718 3399 4455 5501 718
Size: 2681 1056 1046 718
Fragments arranged by size:
2681 1056 1046 718
BclI T'GATC_A
Cuts at: 1605 1826 3021 1605
Size: 221 1195 4085
Fragments arranged by size:
4085 1195 221
BglII A'GATC_T
Cuts at: 3016 5053 3016
Size: 2037 3464
BlpI GC'TnA_GC
Cuts at: 2613 2613
Size: 5501
Bme1580I G_kGCm'C
Cuts at: 197 221 1166 1908 3126 3219 4343 197
Size: 24 945 742 1218 93 1124 1355
Fragments arranged by size:
1355 1218 1124 945 742 93 24
BmgBI CAC'GTC
Cuts at: 1896 1896
Size: 5501
BmrI ACTGGGnnnn_n'
Cuts at: 468 1519 2439 2713 2794 3129 5273 468
Size: 1051 920 274 81 335 2144 696
Fragments arranged by size:
2144 1051 920 696 335 274 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3868 3868
Size: 5501
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 2134 544
Size: 1590 3911
Bpu10I CC'TnA_GC
Cuts at: 491 1951 2371 491
Size: 1460 420 3621
Fragments arranged by size:
3621 1460 420
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2677 3556 4270 4568 2677
Size: 879 714 298 3610
Fragments arranged by size:
3610 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 586 5205 586
Size: 4619 882
BsaAI yAC'GTr
Cuts at: 807 2854 807
Size: 2047 3454
BsaBI GATnn'nnATC
Cuts at: 6 1972 3015 5052 6
Size: 1966 1043 2037 455
Fragments arranged by size:
2037 1966 1043 455
BsaHI Gr'CG_yC
Cuts at: 1961 2932 3180 3882 5378 1961
Size: 971 248 702 1496 2084
Fragments arranged by size:
2084 1496 971 702 248
BsaWI w'CCGG_w
Cuts at: 576 2690 3211 3889 4300 4447 4721 576
Size: 2114 521 678 411 147 274 1356
Fragments arranged by size:
2114 1356 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 517 547 2213 2243 2295 2325 517
Size: 30 1666 30 52 30 3693
Fragments arranged by size:
3693 1666 52 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 313 811 2300 313
Size: 498 1489 3514
Fragments arranged by size:
3514 1489 498
BseYI C'CCAG_C
Cuts at: 269 2926 3684 4349 4757 269
Size: 2657 758 665 408 1013
Fragments arranged by size:
2657 1013 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 1670 571
Size: 1099 4402
BsiEI CG_ry'CG
Cuts at: 104 1374 3043 3089 4319 104
Size: 1270 1669 46 1230 1286
Fragments arranged by size:
1669 1286 1270 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1166 1957 2377 2515 3293 3483 4343 1166
Size: 791 420 138 778 190 860 2324
Fragments arranged by size:
2324 860 791 778 420 190 138
Bsp1286I G_dGCh'C
Cuts at: 197 221 474 1166 1247 1908 1957 2231 2356
Size: 24 253 692 81 661 49 274 125
Cuts at: 2356 2377 2515 3126 3219 3293 3483 3545 4343
Size: 21 138 611 93 74 190 62 798
Cuts at: 4343 197
Size: 1355
Fragments arranged by size:
1355 798 692 661 611 274 253 190
138 125 93 81 74 62 49 24
21
BspEI T'CCGG_A
Cuts at: 576 576
Size: 5501
BspHI T'CATG_A
Cuts at: 3933 3933
Size: 5501
BspMI ACCTGCnnnn'nnnn_
Cuts at: 206 234 2551 2765 3067 3448 206
Size: 28 2317 214 302 381 2259
Fragments arranged by size:
2317 2259 381 302 214 28
BsrBI CCG'CTC
Cuts at: 50 186 3793 3847 5331 5496 50
Size: 136 3607 54 1484 165 55
Fragments arranged by size:
3607 1484 165 136 55 54
BsrDI GCAATG_nn'
Cuts at: 1194 3413 1194
Size: 2219 3282
BsrFI r'CCGG_y
Cuts at: 1945 3499 3680 1945
Size: 1554 181 3766
Fragments arranged by size:
3766 1554 181
BsrGI T'GTAC_A
Cuts at: 701 701
Size: 5501
BssSI C'ACGA_G
Cuts at: 589 2274 3772 4480 589
Size: 1685 1498 708 1610
Fragments arranged by size:
1685 1610 1498 708
BstAPI GCAn_nnn'nTGC
Cuts at: 158 2878 158
Size: 2720 2781
BstBI TT'CG_AA
Cuts at: 3862 3862
Size: 5501
BstXI CCAn_nnnn'nTGG
Cuts at: 453 828 2250 453
Size: 375 1422 3704
Fragments arranged by size:
3704 1422 375
BstYI r'GATC_y
Cuts at: 1 987 2526 2993 3016 3351 3597 3903 3915
Size: 986 1539 467 23 335 246 306 12
Cuts at: 3915 4001 5053 1
Size: 86 1052 449
Fragments arranged by size:
1539 1052 986 467 449 335 306 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 924 924
Size: 5501
BtgI C'CryG_G
Cuts at: 213 1307 2303 213
Size: 1094 996 3411
Fragments arranged by size:
3411 1094 996
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1847 3451 3632 3795 1847
Size: 1604 181 163 3553
Fragments arranged by size:
3553 1604 181 163
BtsI GCAGTG_nn'
Cuts at: 1906 2744 1906
Size: 838 4663
ClaI AT'CG_AT
Cuts at: 1370 1370
Size: 5501
DraI TTT'AAA
Cuts at: 2500 2500
Size: 5501
DraIII CAC_nnn'GTG
Cuts at: 810 810
Size: 5501
DrdI GACnn_nn'nnGTC
Cuts at: 3207 4551 3207
Size: 1344 4157
EaeI y'GGCC_r
Cuts at: 517 1529 1670 2233 2360 2387 3086 3260 3651
Size: 1012 141 563 127 27 699 174 391
Cuts at: 3651 3678 5023 517
Size: 27 1345 995
Fragments arranged by size:
1345 1012 995 699 563 391 174 141
127 27 27
EagI C'GGCC_G
Cuts at: 3086 3086
Size: 5501
EarI CTCTTCn'nnn_
Cuts at: 458 966 3524 3734 458
Size: 508 2558 210 2225
Fragments arranged by size:
2558 2225 508 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 860 3709 4451 4597 5311 860
Size: 2849 742 146 714 1050
Fragments arranged by size:
2849 1050 742 714 146
EcoICRI GAG'CTC
Cuts at: 2375 2513 2375
Size: 138 5363
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 641 1677 2134 3326 3758 4111 4773 544
Size: 97 1036 457 1192 432 353 662 1272
Fragments arranged by size:
1272 1192 1036 662 457 432 353 97
EcoNI CCTnn'n_nnAGG
Cuts at: 1583 1583
Size: 5501
EcoO109I rG'GnC_Cy
Cuts at: 1539 2366 2434 2640 1539
Size: 827 68 206 4400
Fragments arranged by size:
4400 827 206 68
EcoRI G'AATT_C
Cuts at: 538 2505 538
Size: 1967 3534
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1821 3520 1821
Size: 1699 3802
FspI TGC'GCA
Cuts at: 3282 3282
Size: 5501
HaeII r_GCGC'y
Cuts at: 258 692 1964 2242 2935 3183 4413 5184 258
Size: 434 1272 278 693 248 1230 771 575
Fragments arranged by size:
1272 1230 771 693 575 434 278 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 337 369 409 441 473 517 549 2213 2245
Size: 32 40 32 32 44 32 1664 32
Cuts at: 2245 3343 3373 3375 3405 337
Size: 1098 30 2 30 2433
Fragments arranged by size:
2433 1664 1098 44 40 32 32 32
32 32 30 30 2
HincII GTy'rAC
Cuts at: 1551 2540 1551
Size: 989 4512
KasI G'GCGC_C
Cuts at: 1960 2931 3179 1960
Size: 971 248 4282
Fragments arranged by size:
4282 971 248
KpnI G_GTAC'C
Cuts at: 2521 2521
Size: 5501
MfeI C'AATT_G
Cuts at: 1634 1634
Size: 5501
MluI A'CGCG_T
Cuts at: 4934 4934
Size: 5501
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 434 515 1649 4260 4444 434
Size: 81 1134 2611 184 1491
Fragments arranged by size:
2611 1491 1134 184 81
MscI TGG'CCA
Cuts at: 1531 3262 1531
Size: 1731 3770
MslI CAynn'nnrTG
Cuts at: 167 409 484 3490 3617 167
Size: 242 75 3006 127 2051
Fragments arranged by size:
3006 2051 242 127 75
MspA1I CmG'CkG
Cuts at: 1297 2612 2926 3286 4070 4315 1297
Size: 1315 314 360 784 245 2483
Fragments arranged by size:
2483 1315 784 360 314 245
NaeI GCC'GGC
Cuts at: 1947 3682 1947
Size: 1735 3766
NarI GG'CG_CC
Cuts at: 1961 2932 3180 1961
Size: 971 248 4282
Fragments arranged by size:
4282 971 248
NcoI C'CATG_G
Cuts at: 213 213
Size: 5501
NdeI CA'TA_TG
Cuts at: 839 839
Size: 5501
NgoMIV G'CCGG_C
Cuts at: 1945 3680 1945
Size: 1735 3766
NspI r_CATG'y
Cuts at: 156 1327 2217 2554 3054 4657 156
Size: 1171 890 337 500 1603 1000
Fragments arranged by size:
1603 1171 1000 890 500 337
PasI CC'CwG_GG
Cuts at: 955 955
Size: 5501
PciI A'CATG_T
Cuts at: 4653 4653
Size: 5501
PflMI CCAn_nnn'nTGG
Cuts at: 997 1417 997
Size: 420 5081
PfoI T'CCnGG_A
Cuts at: 554 554
Size: 5501
PmeI GTTT'AAAC
Cuts at: 2500 2500
Size: 5501
PmlI CAC'GTG
Cuts at: 807 807
Size: 5501
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3947 3979 3947
Size: 32 5469
PpuMI rG'GwC_Cy
Cuts at: 1539 2366 2434 1539
Size: 827 68 4606
Fragments arranged by size:
4606 827 68
PshAI GACnn'nnGTC
Cuts at: 886 886
Size: 5501
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 358 390 358
Size: 32 5469
PstI C_TGCA'G
Cuts at: 2548 3233 2548
Size: 685 4816
PvuI CG_AT'CG
Cuts at: 104 1374 104
Size: 1270 4231
PvuII CAG'CTG
Cuts at: 1297 2926 3286 1297
Size: 1629 360 3512
Fragments arranged by size:
3512 1629 360
RsrII CG'GwC_CG
Cuts at: 3696 3696
Size: 5501
SacI G_AGCT'C
Cuts at: 2377 2515 2377
Size: 138 5363
SalI G'TCGA_C
Cuts at: 2538 2538
Size: 5501
SanDI GG'GwC_CC
Cuts at: 2366 2366
Size: 5501
SapI GCTCTTCn'nnn_
Cuts at: 3524 3734 3524
Size: 210 5291
SbfI CC_TGCA'GG
Cuts at: 2548 2548
Size: 5501
ScaI AGT'ACT
Cuts at: 5018 5018
Size: 5501
SfcI C'TryA_G
Cuts at: 33 250 1109 1134 2422 2544 3229 4197 4388
Size: 217 859 25 1288 122 685 968 191
Cuts at: 4388 33
Size: 1146
Fragments arranged by size:
1288 1146 968 859 685 217 191 122
25
SfoI GGC'GCC
Cuts at: 1962 2933 3181 1962
Size: 971 248 4282
Fragments arranged by size:
4282 971 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5501
SmaI CCC'GGG
Cuts at: 2523 2523
Size: 5501
SmlI C'TyrA_G
Cuts at: 2656 3571 4285 4547 2656
Size: 915 714 262 3610
Fragments arranged by size:
3610 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5501
SphI G_CATG'C
Cuts at: 156 1327 2554 3054 156
Size: 1171 1227 500 2603
Fragments arranged by size:
2603 1227 1171 500
SspI AAT'ATT
Cuts at: 1026 1026
Size: 5501
StuI AGG'CCT
Cuts at: 1930 1930
Size: 5501
StyI C'CwwG_G
Cuts at: 213 791 821 2635 2989 213
Size: 578 30 1814 354 2725
Fragments arranged by size:
2725 1814 578 354 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 820 898 3084 3217 3676 3885 820
Size: 78 2186 133 459 209 2436
Fragments arranged by size:
2436 2186 459 209 133 78
TatI w'GTAC_w
Cuts at: 302 476 701 2210 5016 302
Size: 174 225 1509 2806 787
Fragments arranged by size:
2806 1509 787 225 174
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1392 1424 2295 2327 5018 5050 1392
Size: 32 871 32 2691 32 1843
Fragments arranged by size:
2691 1843 871 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3298 3298
Size: 5501
XbaI T'CTAG_A
Cuts at: 2532 2532
Size: 5501
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 450 519 1524 2158 2996 450
Size: 69 1005 634 838 2955
Fragments arranged by size:
2955 1005 838 634 69
XmaI C'CCGG_G
Cuts at: 2521 2521
Size: 5501
XmnI GAAnn'nnTTC
Cuts at: 283 1712 283
Size: 1429 4072
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AleI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BfrBI BglI BmtI BsiWI
BsmI BsmBI BssHII BstEII Bsu36I CspCI EcoRV FseI
FspAI HindIII HpaI NheI NotI NruI NsiI PacI
PsiI PspOMI PspXI SacII SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI ZraI