Restriction map for
pF1KB5128
(Circular) (Six-base) MAPSORT of: pF1KB5128.seq Check: 8027 from: 1 to: 5498
pF1KB5128 5498 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1604 1604
Size: 5498
AatII G_ACGT'C
Cuts at: 1436 1436
Size: 5498
AccI GT'mk_AC
Cuts at: 2168 2536 2168
Size: 368 5130
Acc65I G'GTAC_C
Cuts at: 2514 2514
Size: 5498
AclI AA'CG_TT
Cuts at: 5338 5338
Size: 5498
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2425 3323 3755 4108 4770 2425
Size: 898 432 353 662 3153
Fragments arranged by size:
3153 898 662 432 353
AfeI AGC'GCT
Cuts at: 1347 1347
Size: 5498
AflIII A'CryG_T
Cuts at: 2850 4650 4931 2850
Size: 1800 281 3417
Fragments arranged by size:
3417 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 858 2803 4241 858
Size: 1945 1438 2115
Fragments arranged by size:
2115 1945 1438
ApaI G_GGCC'C
Cuts at: 2296 2296
Size: 5498
ApaLI G'TGCA_C
Cuts at: 832 4336 832
Size: 3504 1994
ApoI r'AATT_y
Cuts at: 125 1211 1299 2502 4851 5107 125
Size: 1086 88 1203 2349 256 516
Fragments arranged by size:
2349 1203 1086 516 256 88
AseI AT'TA_AT
Cuts at: 20 20
Size: 5498
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5498
AvaI C'yCGr_G
Cuts at: 187 296 1542 1835 1890 2287 2518 187
Size: 109 1246 293 55 397 231 3167
Fragments arranged by size:
3167 1246 397 293 231 109 55
AvrII C'CTAG_G
Cuts at: 766 766
Size: 5498
BamHI G'GATC_C
Cuts at: 2523 2523
Size: 5498
BanI G'GyrC_C
Cuts at: 1546 1699 1830 2283 2326 2514 2928 3176 3211
Size: 153 131 453 43 188 414 248 35
Cuts at: 3211 1546
Size: 3833
Fragments arranged by size:
3833 453 414 248 188 153 131 43
35
BanII G_rGCy'C
Cuts at: 389 2296 2512 3542 389
Size: 1907 216 1030 2345
Fragments arranged by size:
2345 1907 1030 216
BbeI G_GCGC'C
Cuts at: 1703 2932 3180 1703
Size: 1229 248 4021
Fragments arranged by size:
4021 1229 248
BbsI GAAGACnn'nnnn_
Cuts at: 595 595
Size: 5498
BbvCI CC'TCA_GC
Cuts at: 575 575
Size: 5498
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 319 353 319
Size: 34 5464
BciVI GTATCCnnnnn_n'
Cuts at: 3396 4452 5498 3396
Size: 1056 1046 3396
Fragments arranged by size:
3396 1056 1046
BclI T'GATC_A
Cuts at: 486 797 3018 486
Size: 311 2221 2966
Fragments arranged by size:
2966 2221 311
BglI GCCn_nnn'nGGC
Cuts at: 247 1678 247
Size: 1431 4067
BglII A'GATC_T
Cuts at: 3013 5050 3013
Size: 2037 3461
BlpI GC'TnA_GC
Cuts at: 1993 2610 1993
Size: 617 4881
Bme1580I G_kGCm'C
Cuts at: 836 1549 1835 2288 2296 3123 3216 4340 836
Size: 713 286 453 8 827 93 1124 1994
Fragments arranged by size:
1994 1124 827 713 453 286 93 8
BmrI ACTGGGnnnn_n'
Cuts at: 2710 2791 3126 5270 2710
Size: 81 335 2144 2938
Fragments arranged by size:
2938 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 246 3865 246
Size: 3619 1879
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 368
Size: 5498
Bpu10I CC'TnA_GC
Cuts at: 575 575
Size: 5498
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1909 2674 3553 4267 4565 1909
Size: 765 879 714 298 2842
Fragments arranged by size:
2842 879 765 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1317 1333 1431 1620 5202 1317
Size: 16 98 189 3582 1613
Fragments arranged by size:
3582 1613 189 98 16
BsaAI yAC'GTr
Cuts at: 2082 2851 2082
Size: 769 4729
BsaBI GATnn'nnATC
Cuts at: 6 3012 5049 6
Size: 3006 2037 455
Fragments arranged by size:
3006 2037 455
BsaHI Gr'CG_yC
Cuts at: 1433 1700 2929 3177 3879 5375 1433
Size: 267 1229 248 702 1496 1556
Fragments arranged by size:
1556 1496 1229 702 267 248
BsaWI w'CCGG_w
Cuts at: 1228 2687 3208 3886 4297 4444 4718 1228
Size: 1459 521 678 411 147 274 2008
Fragments arranged by size:
2008 1459 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 727 1091 2265 727
Size: 364 1174 3960
Fragments arranged by size:
3960 1174 364
BseYI C'CCAG_C
Cuts at: 1403 2105 2923 3681 4346 4754 1403
Size: 702 818 758 665 408 2147
Fragments arranged by size:
2147 818 758 702 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 871 1441 1492 1803 2062 2265 709
Size: 162 570 51 311 259 203 3942
Fragments arranged by size:
3942 570 311 259 203 162 51
BsiEI CG_ry'CG
Cuts at: 104 1844 3040 3086 4316 104
Size: 1740 1196 46 1230 1286
Fragments arranged by size:
1740 1286 1230 1196 46
BsiHKAI G_wGCw'C
Cuts at: 389 521 836 2087 2222 2512 3290 3480 4340
Size: 132 315 1251 135 290 778 190 860
Cuts at: 4340 389
Size: 1547
Fragments arranged by size:
1547 1251 860 778 315 290 190 135
132
BsmBI CGTCTCn'nnnn_
Cuts at: 1183 1183
Size: 5498
Bsp1286I G_dGCh'C
Cuts at: 389 521 836 1549 1835 2087 2222 2288 2296
Size: 132 315 713 286 252 135 66 8
Cuts at: 2296 2512 3123 3216 3290 3480 3542 4340 389
Size: 216 611 93 74 190 62 798 1547
Fragments arranged by size:
1547 798 713 611 315 286 252 216
190 135 132 93 74 66 62 8
BspEI T'CCGG_A
Cuts at: 1228 1228
Size: 5498
BspHI T'CATG_A
Cuts at: 3930 3930
Size: 5498
BspMI ACCTGCnnnn'nnnn_
Cuts at: 843 1413 1604 2123 2548 2762 3064 3445 843
Size: 570 191 519 425 214 302 381 2896
Fragments arranged by size:
2896 570 519 425 381 302 214 191
BsrBI CCG'CTC
Cuts at: 50 227 3790 3844 5328 5493 50
Size: 177 3563 54 1484 165 55
Fragments arranged by size:
3563 1484 177 165 55 54
BsrDI GCAATG_nn'
Cuts at: 603 3410 603
Size: 2807 2691
BsrFI r'CCGG_y
Cuts at: 441 3496 3677 441
Size: 3055 181 2262
Fragments arranged by size:
3055 2262 181
BssSI C'ACGA_G
Cuts at: 739 2023 3769 4477 739
Size: 1284 1746 708 1760
Fragments arranged by size:
1760 1746 1284 708
BstAPI GCAn_nnn'nTGC
Cuts at: 327 2875 327
Size: 2548 2950
BstBI TT'CG_AA
Cuts at: 789 1031 3859 789
Size: 242 2828 2428
Fragments arranged by size:
2828 2428 242
BstEII G'GTnAC_C
Cuts at: 758 1560 1860 758
Size: 802 300 4396
Fragments arranged by size:
4396 802 300
BstXI CCAn_nnnn'nTGG
Cuts at: 258 1872 258
Size: 1614 3884
BstYI r'GATC_y
Cuts at: 1 255 2523 2990 3013 3348 3594 3900 3912
Size: 254 2268 467 23 335 246 306 12
Cuts at: 3912 3998 5050 1
Size: 86 1052 449
Fragments arranged by size:
2268 1052 467 449 335 306 254 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1651 2126 1651
Size: 475 5023
BtgI C'CryG_G
Cuts at: 1710 1931 1710
Size: 221 5277
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1350 3448 3629 3792 1350
Size: 2098 181 163 3056
Fragments arranged by size:
3056 2098 181 163
BtsI GCAGTG_nn'
Cuts at: 1524 2741 1524
Size: 1217 4281
DraI TTT'AAA
Cuts at: 2497 2497
Size: 5498
DrdI GACnn_nn'nnGTC
Cuts at: 3204 4548 3204
Size: 1344 4154
EaeI y'GGCC_r
Cuts at: 248 1635 1841 2063 3083 3257 3648 3675 5020
Size: 1387 206 222 1020 174 391 27 1345
Cuts at: 5020 248
Size: 726
Fragments arranged by size:
1387 1345 1020 726 391 222 206 174
27
EagI C'GGCC_G
Cuts at: 1841 3083 1841
Size: 1242 4256
EarI CTCTTCn'nnn_
Cuts at: 377 2209 2215 3521 3731 377
Size: 1832 6 1306 210 2144
Fragments arranged by size:
2144 1832 1306 210 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3706 4448 4594 5308 3706
Size: 742 146 714 3896
Fragments arranged by size:
3896 742 714 146
EcoICRI GAG'CTC
Cuts at: 387 2510 387
Size: 2123 3375
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 2425 3323 3755 4108 4770 368
Size: 2057 898 432 353 662 1096
Fragments arranged by size:
2057 1096 898 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 855 1587 1671 2292 2293 2333 2637 855
Size: 732 84 621 1 40 304 3716
Fragments arranged by size:
3716 732 621 304 84 40 1
EcoRI G'AATT_C
Cuts at: 125 1211 2502 125
Size: 1086 1291 3121
Fragments arranged by size:
3121 1291 1086
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3517 3517
Size: 5498
FspI TGC'GCA
Cuts at: 1978 3279 1978
Size: 1301 4197
FspAI rTGC'GCAy
Cuts at: 1978 1978
Size: 5498
HaeII r_GCGC'y
Cuts at: 748 1349 1529 1703 1741 2932 3180 4410 5181
Size: 601 180 174 38 1191 248 1230 771
Cuts at: 5181 748
Size: 1065
Fragments arranged by size:
1230 1191 1065 771 601 248 180 174
38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 114 146 742 774 1525 1557 1873 1905 2086
Size: 32 596 32 751 32 316 32 181
Cuts at: 2086 2118 3340 3370 3372 3402 114
Size: 32 1222 30 2 30 2210
Fragments arranged by size:
2210 1222 751 596 316 181 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2537 2537
Size: 5498
KasI G'GCGC_C
Cuts at: 1699 2928 3176 1699
Size: 1229 248 4021
Fragments arranged by size:
4021 1229 248
KpnI G_GTAC'C
Cuts at: 2518 2518
Size: 5498
MfeI C'AATT_G
Cuts at: 884 884
Size: 5498
MluI A'CGCG_T
Cuts at: 4931 4931
Size: 5498
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 191 1454 4257 4441 191
Size: 1263 2803 184 1248
Fragments arranged by size:
2803 1263 1248 184
MscI TGG'CCA
Cuts at: 250 3259 250
Size: 3009 2489
MslI CAynn'nnrTG
Cuts at: 781 1111 1417 3487 3614 781
Size: 330 306 2070 127 2665
Fragments arranged by size:
2665 2070 330 306 127
MspA1I CmG'CkG
Cuts at: 333 1712 1797 1920 2609 2923 3283 4067 4312
Size: 1379 85 123 689 314 360 784 245
Cuts at: 4312 333
Size: 1519
Fragments arranged by size:
1519 1379 784 689 360 314 245 123
85
NaeI GCC'GGC
Cuts at: 3679 3679
Size: 5498
NarI GG'CG_CC
Cuts at: 1700 2929 3177 1700
Size: 1229 248 4021
Fragments arranged by size:
4021 1229 248
NdeI CA'TA_TG
Cuts at: 843 843
Size: 5498
NgoMIV G'CCGG_C
Cuts at: 3677 3677
Size: 5498
NspI r_CATG'y
Cuts at: 2551 3051 4654 2551
Size: 500 1603 3395
Fragments arranged by size:
3395 1603 500
PciI A'CATG_T
Cuts at: 4650 4650
Size: 5498
PfoI T'CCnGG_A
Cuts at: 431 473 857 431
Size: 42 384 5072
Fragments arranged by size:
5072 384 42
PmeI GTTT'AAAC
Cuts at: 2497 2497
Size: 5498
PmlI CAC'GTG
Cuts at: 2082 2082
Size: 5498
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2383 2415 3944 3976 2383
Size: 32 1529 32 3905
Fragments arranged by size:
3905 1529 32 32
PpuMI rG'GwC_Cy
Cuts at: 855 1587 2333 855
Size: 732 746 4020
Fragments arranged by size:
4020 746 732
PshAI GACnn'nnGTC
Cuts at: 1195 1195
Size: 5498
PspOMI G'GGCC_C
Cuts at: 2292 2292
Size: 5498
PspXI vC'TCGA_Gb
Cuts at: 187 187
Size: 5498
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 395 427 395
Size: 32 5466
PstI C_TGCA'G
Cuts at: 1679 1802 2545 3230 1679
Size: 123 743 685 3947
Fragments arranged by size:
3947 743 685 123
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5498
PvuII CAG'CTG
Cuts at: 333 1920 2923 3283 333
Size: 1587 1003 360 2548
Fragments arranged by size:
2548 1587 1003 360
RsrII CG'GwC_CG
Cuts at: 3693 3693
Size: 5498
SacI G_AGCT'C
Cuts at: 389 2512 389
Size: 2123 3375
SacII CC_GC'GG
Cuts at: 1713 1713
Size: 5498
SalI G'TCGA_C
Cuts at: 2535 2535
Size: 5498
SanDI GG'GwC_CC
Cuts at: 2333 2333
Size: 5498
SapI GCTCTTCn'nnn_
Cuts at: 377 2209 3521 3731 377
Size: 1832 1312 210 2144
Fragments arranged by size:
2144 1832 1312 210
SbfI CC_TGCA'GG
Cuts at: 1679 2545 1679
Size: 866 4632
ScaI AGT'ACT
Cuts at: 5015 5015
Size: 5498
SexAI A'CCwGG_T
Cuts at: 1856 2171 1856
Size: 315 5183
SfcI C'TryA_G
Cuts at: 33 1100 1675 1798 2541 3226 4194 4385 33
Size: 1067 575 123 743 685 968 191 1146
Fragments arranged by size:
1146 1067 968 743 685 575 191 123
SfoI GGC'GCC
Cuts at: 1701 2930 3178 1701
Size: 1229 248 4021
Fragments arranged by size:
4021 1229 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5498
SmaI CCC'GGG
Cuts at: 298 1837 2520 298
Size: 1539 683 3276
Fragments arranged by size:
3276 1539 683
SmlI C'TyrA_G
Cuts at: 187 1924 2653 3568 4282 4544 187
Size: 1737 729 915 714 262 1141
Fragments arranged by size:
1737 1141 915 729 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5498
SphI G_CATG'C
Cuts at: 2551 3051 2551
Size: 500 4998
SspI AAT'ATT
Cuts at: 500 2414 500
Size: 1914 3584
StuI AGG'CCT
Cuts at: 1082 1082
Size: 5498
StyI C'CwwG_G
Cuts at: 251 766 1865 2632 2986 251
Size: 515 1099 767 354 2763
Fragments arranged by size:
2763 1099 767 515 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1349 1404 1474 3081 3214 3673 3882 1349
Size: 55 70 1607 133 459 209 2965
Fragments arranged by size:
2965 1607 459 209 133 70 55
TatI w'GTAC_w
Cuts at: 411 1352 5013 411
Size: 941 3661 896
Fragments arranged by size:
3661 941 896
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5015 5047 5015
Size: 32 5466
Tth111I GACn'n_nGTC
Cuts at: 2337 3295 2337
Size: 958 4540
XbaI T'CTAG_A
Cuts at: 2529 2529
Size: 5498
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2993 2993
Size: 5498
XhoI C'TCGA_G
Cuts at: 187 187
Size: 5498
XmaI C'CCGG_G
Cuts at: 296 1835 2518 296
Size: 1539 683 3276
Fragments arranged by size:
3276 1539 683
ZraI GAC'GTC
Cuts at: 1434 1434
Size: 5498
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NspI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AleI AlfI AloI AscI BaeI
BfrBI BmgBI BmtI BsaXI BsiWI BsmI BsrGI BssHII
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI HindIII
HpaI NcoI NheI NotI NruI NsiI PacI PasI
PflMI PsiI SfiI SgrAI SnaBI SrfI SwaI XmnI