Restriction map for
pF1KB5103
(Circular) (Six-base) MAPSORT of: pF1KB5103.seq Check: 5203 from: 1 to: 5213
pF1KB5103 5213 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 1595 2251 1595
Size: 656 4557
Acc65I G'GTAC_C
Cuts at: 2229 2229
Size: 5213
AclI AA'CG_TT
Cuts at: 5053 5053
Size: 5213
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 896 1907 3038 3470 3823 4485 133
Size: 763 1011 1131 432 353 662 861
Fragments arranged by size:
1131 1011 861 763 662 432 353
AflIII A'CryG_T
Cuts at: 1307 1634 2565 4365 4646 1307
Size: 327 931 1800 281 1874
Fragments arranged by size:
1874 1800 931 327 281
AlwNI CAG_nnn'CTG
Cuts at: 1917 1940 2518 3956 1917
Size: 23 578 1438 3174
Fragments arranged by size:
3174 1438 578 23
ApaLI G'TGCA_C
Cuts at: 443 4051 443
Size: 3608 1605
ApoI r'AATT_y
Cuts at: 547 884 1499 1552 1656 2217 4566 4822 547
Size: 337 615 53 104 561 2349 256 938
Fragments arranged by size:
2349 938 615 561 337 256 104 53
AseI AT'TA_AT
Cuts at: 20 20
Size: 5213
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5213
AvaI C'yCGr_G
Cuts at: 360 2233 360
Size: 1873 3340
BamHI G'GATC_C
Cuts at: 2238 2238
Size: 5213
BanI G'GyrC_C
Cuts at: 221 371 799 2229 2643 2891 2926 221
Size: 150 428 1430 414 248 35 2508
Fragments arranged by size:
2508 1430 428 414 248 150 35
BanII G_rGCy'C
Cuts at: 1394 2227 3257 1394
Size: 833 1030 3350
Fragments arranged by size:
3350 1030 833
BbeI G_GCGC'C
Cuts at: 375 2647 2895 375
Size: 2272 248 2693
Fragments arranged by size:
2693 2272 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1363 1397 90
Size: 34 1239 34 3906
Fragments arranged by size:
3906 1239 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1095 3111 4167 5213 1095
Size: 2016 1056 1046 1095
Fragments arranged by size:
2016 1095 1056 1046
BclI T'GATC_A
Cuts at: 2154 2733 2154
Size: 579 4634
BglI GCCn_nnn'nGGC
Cuts at: 1193 1193
Size: 5213
BglII A'GATC_T
Cuts at: 2728 4765 2728
Size: 2037 3176
BlpI GC'TnA_GC
Cuts at: 961 2325 961
Size: 1364 3849
Bme1580I G_kGCm'C
Cuts at: 447 804 2838 2931 4055 447
Size: 357 2034 93 1124 1605
Fragments arranged by size:
2034 1605 1124 357 93
BmrI ACTGGGnnnn_n'
Cuts at: 2425 2506 2841 4985 2425
Size: 81 335 2144 2653
Fragments arranged by size:
2653 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3580 3580
Size: 5213
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 397 448 1654 1917 304
Size: 93 51 1206 263 3600
Fragments arranged by size:
3600 1206 263 93 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2389 3268 3982 4280 2389
Size: 879 714 298 3322
Fragments arranged by size:
3322 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 500 4917 500
Size: 4417 796
BsaAI yAC'GTr
Cuts at: 1635 2566 1635
Size: 931 4282
BsaBI GATnn'nnATC
Cuts at: 6 1424 2727 4764 6
Size: 1418 1303 2037 455
Fragments arranged by size:
2037 1418 1303 455
BsaHI Gr'CG_yC
Cuts at: 372 2644 2892 3594 5090 372
Size: 2272 248 702 1496 495
Fragments arranged by size:
2272 1496 702 495 248
BsaWI w'CCGG_w
Cuts at: 1750 2402 2923 3601 4012 4159 4433 1750
Size: 652 521 678 411 147 274 2530
Fragments arranged by size:
2530 678 652 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 228 956 228
Size: 728 4485
BseYI C'CCAG_C
Cuts at: 278 1991 2638 3396 4061 4469 278
Size: 1713 647 758 665 408 1022
Fragments arranged by size:
1713 1022 758 665 647 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1892 1941 1892
Size: 49 5164
BsiEI CG_ry'CG
Cuts at: 104 2755 2801 4031 104
Size: 2651 46 1230 1286
Fragments arranged by size:
2651 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 447 1933 2227 3005 3195 4055 447
Size: 1486 294 778 190 860 1605
Fragments arranged by size:
1605 1486 860 778 294 190
BsmI GAATG_Cn'
Cuts at: 970 1891 970
Size: 921 4292
BsmBI CGTCTCn'nnnn_
Cuts at: 835 835
Size: 5213
Bsp1286I G_dGCh'C
Cuts at: 447 804 1394 1933 2227 2838 2931 3005 3195
Size: 357 590 539 294 611 93 74 190
Cuts at: 3195 3257 4055 447
Size: 62 798 1605
Fragments arranged by size:
1605 798 611 590 539 357 294 190
93 74 62
BspHI T'CATG_A
Cuts at: 1050 3645 1050
Size: 2595 2618
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1914 2134 2263 2477 2779 3160 1914
Size: 220 129 214 302 381 3967
Fragments arranged by size:
3967 381 302 220 214 129
BsrBI CCG'CTC
Cuts at: 50 203 3505 3559 5043 5208 50
Size: 153 3302 54 1484 165 55
Fragments arranged by size:
3302 1484 165 153 55 54
BsrDI GCAATG_nn'
Cuts at: 3125 3125
Size: 5213
BsrFI r'CCGG_y
Cuts at: 3211 3392 3211
Size: 181 5032
BsrGI T'GTAC_A
Cuts at: 1202 1202
Size: 5213
BssSI C'ACGA_G
Cuts at: 3484 4192 3484
Size: 708 4505
BstAPI GCAn_nnn'nTGC
Cuts at: 2590 2590
Size: 5213
BstBI TT'CG_AA
Cuts at: 3574 3574
Size: 5213
BstYI r'GATC_y
Cuts at: 1 1857 2187 2238 2705 2728 3063 3309 3615
Size: 1856 330 51 467 23 335 246 306
Cuts at: 3615 3627 3713 4765 1
Size: 12 86 1052 449
Fragments arranged by size:
1856 1052 467 449 335 330 306 246
86 51 23 12
BstZ17I GTA'TAC
Cuts at: 1596 1596
Size: 5213
Bsu36I CC'TnA_GG
Cuts at: 1880 1880
Size: 5213
BtgI C'CryG_G
Cuts at: 146 1895 1964 146
Size: 1749 69 3395
Fragments arranged by size:
3395 1749 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3163 3344 3507 3163
Size: 181 163 4869
Fragments arranged by size:
4869 181 163
BtsI GCAGTG_nn'
Cuts at: 1699 2456 1699
Size: 757 4456
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1015 1050 1015
Size: 35 5178
DraI TTT'AAA
Cuts at: 2212 2212
Size: 5213
DrdI GACnn_nn'nnGTC
Cuts at: 2919 4263 2919
Size: 1344 3869
EaeI y'GGCC_r
Cuts at: 143 2798 2972 3363 3390 4735 143
Size: 2655 174 391 27 1345 621
Fragments arranged by size:
2655 1345 621 391 174 27
EagI C'GGCC_G
Cuts at: 2798 2798
Size: 5213
EarI CTCTTCn'nnn_
Cuts at: 719 838 3236 3446 719
Size: 119 2398 210 2486
Fragments arranged by size:
2486 2398 210 119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3421 4163 4309 5023 3421
Size: 742 146 714 3611
Fragments arranged by size:
3611 742 714 146
EcoICRI GAG'CTC
Cuts at: 2225 2225
Size: 5213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 304 397 448 896 1654 1907 1917 3038
Size: 171 93 51 448 758 253 10 1121
Cuts at: 3038 3470 3823 4485 133
Size: 432 353 662 861
Fragments arranged by size:
1121 861 758 662 448 432 353 253
171 93 51 10
EcoO109I rG'GnC_Cy
Cuts at: 1730 2352 1730
Size: 622 4591
EcoRI G'AATT_C
Cuts at: 547 1499 1552 2217 547
Size: 952 53 665 3543
Fragments arranged by size:
3543 952 665 53
EcoRV GAT'ATC
Cuts at: 2089 2089
Size: 5213
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2107 3232 2107
Size: 1125 4088
FspI TGC'GCA
Cuts at: 2994 2994
Size: 5213
HaeII r_GCGC'y
Cuts at: 249 375 2647 2895 4125 4896 249
Size: 126 2272 248 1230 771 566
Fragments arranged by size:
2272 1230 771 566 248 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1163 1195 1812 1844 2158 2190 3055 3085 3087
Size: 32 617 32 314 32 865 30 2
Cuts at: 3087 3117 1163
Size: 30 3259
Fragments arranged by size:
3259 865 617 314 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2252 2252
Size: 5213
KasI G'GCGC_C
Cuts at: 371 2643 2891 371
Size: 2272 248 2693
Fragments arranged by size:
2693 2272 248
KpnI G_GTAC'C
Cuts at: 2233 2233
Size: 5213
MluI A'CGCG_T
Cuts at: 4646 4646
Size: 5213
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3972 4156 3972
Size: 184 5029
MscI TGG'CCA
Cuts at: 2974 2974
Size: 5213
MslI CAynn'nnrTG
Cuts at: 1306 2045 2167 3202 3329 1306
Size: 739 122 1035 127 3190
Fragments arranged by size:
3190 1035 739 127 122
MspA1I CmG'CkG
Cuts at: 282 2324 2638 2998 3782 4027 282
Size: 2042 314 360 784 245 1468
Fragments arranged by size:
2042 1468 784 360 314 245
NaeI GCC'GGC
Cuts at: 3394 3394
Size: 5213
NarI GG'CG_CC
Cuts at: 372 2644 2892 372
Size: 2272 248 2693
Fragments arranged by size:
2693 2272 248
NgoMIV G'CCGG_C
Cuts at: 3392 3392
Size: 5213
NspI r_CATG'y
Cuts at: 1311 2036 2266 2766 4369 1311
Size: 725 230 500 1603 2155
Fragments arranged by size:
2155 1603 725 500 230
PciI A'CATG_T
Cuts at: 1307 4365 1307
Size: 3058 2155
PmeI GTTT'AAAC
Cuts at: 2212 2212
Size: 5213
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3659 3691 3659
Size: 32 5181
PpuMI rG'GwC_Cy
Cuts at: 1730 1730
Size: 5213
PstI C_TGCA'G
Cuts at: 1984 2011 2260 2945 1984
Size: 27 249 685 4252
Fragments arranged by size:
4252 685 249 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5213
PvuII CAG'CTG
Cuts at: 282 2638 2998 282
Size: 2356 360 2497
Fragments arranged by size:
2497 2356 360
RsrII CG'GwC_CG
Cuts at: 3408 3408
Size: 5213
SacI G_AGCT'C
Cuts at: 2227 2227
Size: 5213
SalI G'TCGA_C
Cuts at: 2250 2250
Size: 5213
SapI GCTCTTCn'nnn_
Cuts at: 3236 3446 3236
Size: 210 5003
SbfI CC_TGCA'GG
Cuts at: 2260 2260
Size: 5213
ScaI AGT'ACT
Cuts at: 515 4730 515
Size: 4215 998
SfcI C'TryA_G
Cuts at: 33 1504 1734 1980 2007 2256 2941 3909 4100
Size: 1471 230 246 27 249 685 968 191
Cuts at: 4100 33
Size: 1146
Fragments arranged by size:
1471 1146 968 685 249 246 230 191
27
SfoI GGC'GCC
Cuts at: 373 2645 2893 373
Size: 2272 248 2693
Fragments arranged by size:
2693 2272 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5213
SmaI CCC'GGG
Cuts at: 362 2235 362
Size: 1873 3340
SmlI C'TyrA_G
Cuts at: 2368 3283 3997 4259 2368
Size: 915 714 262 3322
Fragments arranged by size:
3322 915 714 262
SpeI A'CTAG_T
Cuts at: 67 1723 67
Size: 1656 3557
SphI G_CATG'C
Cuts at: 2266 2766 2266
Size: 500 4713
StyI C'CwwG_G
Cuts at: 1333 2347 2701 1333
Size: 1014 354 3845
Fragments arranged by size:
3845 1014 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2076 2796 2929 3388 3597 2076
Size: 720 133 459 209 3692
Fragments arranged by size:
3692 720 459 209 133
TatI w'GTAC_w
Cuts at: 513 1202 4728 513
Size: 689 3526 998
Fragments arranged by size:
3526 998 689
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1619 1651 4730 4762 1619
Size: 32 3079 32 2070
Fragments arranged by size:
3079 2070 32 32
Tth111I GACn'n_nGTC
Cuts at: 3010 3010
Size: 5213
XbaI T'CTAG_A
Cuts at: 2244 2244
Size: 5213
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1831 2708 1831
Size: 877 4336
XmaI C'CCGG_G
Cuts at: 360 2233 360
Size: 1873 3340
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbsI BbvCI BfrBI
BmgBI BmtI Bpu10I BsaXI BsiWI BspEI BssHII BstEII
BstXI ClaI DraIII EcoNI FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI