Restriction map for
pF1KB5099
(Circular) (Six-base) MAPSORT of: pF1KB5099.seq Check: 1717 from: 1 to: 5423
pF1KB5099 5423 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 2461 2461
Size: 5423
Acc65I G'GTAC_C
Cuts at: 2439 2439
Size: 5423
AclI AA'CG_TT
Cuts at: 2417 5263 2417
Size: 2846 2577
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 1532 1781 1789 2389 3248 3680 4033 4695
Size: 784 249 8 600 859 432 353 662
Cuts at: 4695 748
Size: 1476
Fragments arranged by size:
1476 859 784 662 600 432 353 249
8
AflII C'TTAA_G
Cuts at: 1177 2299 1177
Size: 1122 4301
AflIII A'CryG_T
Cuts at: 2775 4575 4856 2775
Size: 1800 281 3342
Fragments arranged by size:
3342 1800 281
AleI CACnn'nnGTG
Cuts at: 536 536
Size: 5423
AlwNI CAG_nnn'CTG
Cuts at: 2728 4166 2728
Size: 1438 3985
ApaI G_GGCC'C
Cuts at: 2310 2310
Size: 5423
ApaLI G'TGCA_C
Cuts at: 4261 4261
Size: 5423
ApoI r'AATT_y
Cuts at: 283 522 748 768 1113 1327 1649 2427 4776
Size: 239 226 20 345 214 322 778 2349
Cuts at: 4776 5032 283
Size: 256 674
Fragments arranged by size:
2349 778 674 345 322 256 239 226
214 20
AseI AT'TA_AT
Cuts at: 20 20
Size: 5423
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5423
AvaI C'yCGr_G
Cuts at: 2443 2443
Size: 5423
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 699 732 699
Size: 33 5390
BamHI G'GATC_C
Cuts at: 2448 2448
Size: 5423
BanI G'GyrC_C
Cuts at: 2439 2853 3101 3136 2439
Size: 414 248 35 4726
Fragments arranged by size:
4726 414 248 35
BanII G_rGCy'C
Cuts at: 2310 2437 3467 2310
Size: 127 1030 4266
Fragments arranged by size:
4266 1030 127
BbeI G_GCGC'C
Cuts at: 2857 3105 2857
Size: 248 5175
BciVI GTATCCnnnnn_n'
Cuts at: 1486 3321 4377 5423 1486
Size: 1835 1056 1046 1486
Fragments arranged by size:
1835 1486 1056 1046
BclI T'GATC_A
Cuts at: 2943 2943
Size: 5423
BfrBI ATG'CAT
Cuts at: 292 1887 292
Size: 1595 3828
BglII A'GATC_T
Cuts at: 2938 4975 2938
Size: 2037 3386
BlpI GC'TnA_GC
Cuts at: 2535 2535
Size: 5423
Bme1580I G_kGCm'C
Cuts at: 2310 3048 3141 4265 2310
Size: 738 93 1124 3468
Fragments arranged by size:
3468 1124 738 93
BmrI ACTGGGnnnn_n'
Cuts at: 248 2635 2716 3051 5195 248
Size: 2387 81 335 2144 476
Fragments arranged by size:
2387 2144 476 335 81
BmtI G_CTAG'C
Cuts at: 145 145
Size: 5423
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3790 3790
Size: 5423
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 530 530
Size: 5423
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 428 1594 2599 3478 4192 4490 428
Size: 1166 1005 879 714 298 1361
Fragments arranged by size:
1361 1166 1005 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5127 5127
Size: 5423
BsaAI yAC'GTr
Cuts at: 2776 2776
Size: 5423
BsaBI GATnn'nnATC
Cuts at: 6 1961 2937 4974 6
Size: 1955 976 2037 455
Fragments arranged by size:
2037 1955 976 455
BsaHI Gr'CG_yC
Cuts at: 2854 3102 3804 5300 2854
Size: 248 702 1496 2977
Fragments arranged by size:
2977 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2612 3133 3811 4222 4369 4643 2612
Size: 521 678 411 147 274 3392
Fragments arranged by size:
3392 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1706 1706
Size: 5423
BseYI C'CCAG_C
Cuts at: 1828 2261 2848 3606 4271 4679 1828
Size: 433 587 758 665 408 2572
Fragments arranged by size:
2572 758 665 587 433 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 138 495 138
Size: 357 5066
BsiEI CG_ry'CG
Cuts at: 104 2965 3011 4241 104
Size: 2861 46 1230 1286
Fragments arranged by size:
2861 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 371 2239 2437 3215 3405 4265 371
Size: 1868 198 778 190 860 1529
Fragments arranged by size:
1868 1529 860 778 198 190
BsmI GAATG_Cn'
Cuts at: 432 2340 432
Size: 1908 3515
Bsp1286I G_dGCh'C
Cuts at: 371 2239 2310 2437 3048 3141 3215 3405 3467
Size: 1868 71 127 611 93 74 190 62
Cuts at: 3467 4265 371
Size: 798 1529
Fragments arranged by size:
1868 1529 798 611 190 127 93 74
71 62
BspHI T'CATG_A
Cuts at: 659 737 1953 3855 659
Size: 78 1216 1902 2227
Fragments arranged by size:
2227 1902 1216 78
BspMI ACCTGCnnnn'nnnn_
Cuts at: 983 1023 1806 2161 2256 2473 2687 2989 3370
Size: 40 783 355 95 217 214 302 381
Cuts at: 3370 983
Size: 3036
Fragments arranged by size:
3036 783 381 355 302 217 214 95
40
BsrBI CCG'CTC
Cuts at: 50 3715 3769 5253 5418 50
Size: 3665 54 1484 165 55
Fragments arranged by size:
3665 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1647 1945 2005 2342 3335 1647
Size: 298 60 337 993 3735
Fragments arranged by size:
3735 993 337 298 60
BsrFI r'CCGG_y
Cuts at: 677 3421 3602 677
Size: 2744 181 2498
Fragments arranged by size:
2744 2498 181
BssSI C'ACGA_G
Cuts at: 3694 4402 3694
Size: 708 4715
BstAPI GCAn_nnn'nTGC
Cuts at: 2800 2800
Size: 5423
BstBI TT'CG_AA
Cuts at: 3784 3784
Size: 5423
BstYI r'GATC_y
Cuts at: 1 249 1962 2448 2915 2938 3273 3519 3825
Size: 248 1713 486 467 23 335 246 306
Cuts at: 3825 3837 3923 4975 1
Size: 12 86 1052 449
Fragments arranged by size:
1713 1052 486 467 449 335 306 248
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2146 2146
Size: 5423
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3373 3554 3717 3373
Size: 181 163 5079
Fragments arranged by size:
5079 181 163
BtsI GCAGTG_nn'
Cuts at: 371 803 887 2666 371
Size: 432 84 1779 3128
Fragments arranged by size:
3128 1779 432 84
DraI TTT'AAA
Cuts at: 1431 1655 2422 1431
Size: 224 767 4432
Fragments arranged by size:
4432 767 224
DrdI GACnn_nn'nnGTC
Cuts at: 3129 4473 3129
Size: 1344 4079
EaeI y'GGCC_r
Cuts at: 3008 3182 3573 3600 4945 3008
Size: 174 391 27 1345 3486
Fragments arranged by size:
3486 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3008 3008
Size: 5423
EarI CTCTTCn'nnn_
Cuts at: 3446 3656 3446
Size: 210 5213
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1285 3631 4373 4519 5233 1285
Size: 2346 742 146 714 1475
Fragments arranged by size:
2346 1475 742 714 146
EcoICRI GAG'CTC
Cuts at: 2435 2435
Size: 5423
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 530 748 1532 1781 1789 2389 3248 3680 4033
Size: 218 784 249 8 600 859 432 353
Cuts at: 4033 4695 530
Size: 662 1258
Fragments arranged by size:
1258 859 784 662 600 432 353 249
218 8
EcoO109I rG'GnC_Cy
Cuts at: 2150 2184 2306 2307 2562 2150
Size: 34 122 1 255 5011
Fragments arranged by size:
5011 255 122 34 1
EcoRI G'AATT_C
Cuts at: 522 2427 522
Size: 1905 3518
EcoRV GAT'ATC
Cuts at: 735 735
Size: 5423
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1125 3442 1125
Size: 2317 3106
FspI TGC'GCA
Cuts at: 2157 3204 2157
Size: 1047 4376
HaeII r_GCGC'y
Cuts at: 2857 3105 4335 5106 2857
Size: 248 1230 771 3174
Fragments arranged by size:
3174 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1067 1099 3265 3295 3297 3327 1067
Size: 32 2166 30 2 30 3163
Fragments arranged by size:
3163 2166 32 30 30 2
HincII GTy'rAC
Cuts at: 2462 2462
Size: 5423
HindIII A'AGCT_T
Cuts at: 500 851 500
Size: 351 5072
KasI G'GCGC_C
Cuts at: 2853 3101 2853
Size: 248 5175
KpnI G_GTAC'C
Cuts at: 2443 2443
Size: 5423
MfeI C'AATT_G
Cuts at: 1673 1673
Size: 5423
MluI A'CGCG_T
Cuts at: 4856 4856
Size: 5423
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1877 1990 4182 4366 1877
Size: 113 2192 184 2934
Fragments arranged by size:
2934 2192 184 113
MscI TGG'CCA
Cuts at: 3184 3184
Size: 5423
MslI CAynn'nnrTG
Cuts at: 536 875 3412 3539 536
Size: 339 2537 127 2420
Fragments arranged by size:
2537 2420 339 127
MspA1I CmG'CkG
Cuts at: 1921 2534 2848 3208 3992 4237 1921
Size: 613 314 360 784 245 3107
Fragments arranged by size:
3107 784 613 360 314 245
NaeI GCC'GGC
Cuts at: 3604 3604
Size: 5423
NarI GG'CG_CC
Cuts at: 2854 3102 2854
Size: 248 5175
NgoMIV G'CCGG_C
Cuts at: 3602 3602
Size: 5423
NheI G'CTAG_C
Cuts at: 141 141
Size: 5423
NsiI A_TGCA'T
Cuts at: 294 1889 294
Size: 1595 3828
NspI r_CATG'y
Cuts at: 2476 2976 4579 2476
Size: 500 1603 3320
Fragments arranged by size:
3320 1603 500
PciI A'CATG_T
Cuts at: 4575 4575
Size: 5423
PfoI T'CCnGG_A
Cuts at: 546 546
Size: 5423
PmeI GTTT'AAAC
Cuts at: 2422 2422
Size: 5423
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3869 3901 3869
Size: 32 5391
PpuMI rG'GwC_Cy
Cuts at: 2150 2184 2150
Size: 34 5389
PsiI TTA'TAA
Cuts at: 1439 1439
Size: 5423
PspOMI G'GGCC_C
Cuts at: 2306 2306
Size: 5423
PstI C_TGCA'G
Cuts at: 810 2470 3155 810
Size: 1660 685 3078
Fragments arranged by size:
3078 1660 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5423
PvuII CAG'CTG
Cuts at: 1921 2848 3208 1921
Size: 927 360 4136
Fragments arranged by size:
4136 927 360
RsrII CG'GwC_CG
Cuts at: 3618 3618
Size: 5423
SacI G_AGCT'C
Cuts at: 2437 2437
Size: 5423
SalI G'TCGA_C
Cuts at: 2460 2460
Size: 5423
SapI GCTCTTCn'nnn_
Cuts at: 3446 3656 3446
Size: 210 5213
SbfI CC_TGCA'GG
Cuts at: 2470 2470
Size: 5423
ScaI AGT'ACT
Cuts at: 4940 4940
Size: 5423
SfcI C'TryA_G
Cuts at: 33 806 2466 3151 4119 4310 33
Size: 773 1660 685 968 191 1146
Fragments arranged by size:
1660 1146 968 773 685 191
SfoI GGC'GCC
Cuts at: 2855 3103 2855
Size: 248 5175
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5423
SmaI CCC'GGG
Cuts at: 2445 2445
Size: 5423
SmlI C'TyrA_G
Cuts at: 443 1177 1573 2299 2578 3493 4207 4469 443
Size: 734 396 726 279 915 714 262 1397
Fragments arranged by size:
1397 915 734 726 714 396 279 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5423
SphI G_CATG'C
Cuts at: 2476 2976 2476
Size: 500 4923
SspI AAT'ATT
Cuts at: 1315 1426 1315
Size: 111 5312
StyI C'CwwG_G
Cuts at: 828 2557 2911 828
Size: 1729 354 3340
Fragments arranged by size:
3340 1729 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3006 3139 3598 3807 3006
Size: 133 459 209 4622
Fragments arranged by size:
4622 459 209 133
TatI w'GTAC_w
Cuts at: 439 1390 2046 2088 4938 439
Size: 951 656 42 2850 924
Fragments arranged by size:
2850 951 924 656 42
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4940 4972 4940
Size: 32 5391
Tth111I GACn'n_nGTC
Cuts at: 997 3220 997
Size: 2223 3200
XbaI T'CTAG_A
Cuts at: 2454 2454
Size: 5423
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2918 2918
Size: 5423
XmaI C'CCGG_G
Cuts at: 2443 2443
Size: 5423
XmnI GAAnn'nnTTC
Cuts at: 503 1241 1563 503
Size: 738 322 4363
Fragments arranged by size:
4363 738 322
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NheI NsiI NspI PciI PfoI PmeI
PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AlfI AloI AscI
AvrII BbsI BbvCI BcgI BglI BmgBI Bpu10I BsaXI
BsiWI BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I
BtgI ClaI CspCI DraIII EcoNI FseI FspAI HpaI
NcoI NdeI NotI NruI PacI PasI PflMI PmlI
PshAI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI