Restriction map for
pF1KB5098
(Circular) (Six-base) MAPSORT of: pF1KB5098.seq Check: 495 from: 1 to: 6587
pF1KB5098 6587 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2805 2805
Size: 6587
AccI GT'mk_AC
Cuts at: 1742 3625 1742
Size: 1883 4704
Acc65I G'GTAC_C
Cuts at: 3603 3603
Size: 6587
AclI AA'CG_TT
Cuts at: 6427 6427
Size: 6587
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1144 1524 2673 4412 4844 5591 5859 1144
Size: 380 1149 1739 432 747 268 1872
Fragments arranged by size:
1872 1739 1149 747 432 380 268
AflIII A'CryG_T
Cuts at: 2061 3939 5043 6020 2061
Size: 1878 1104 977 2628
Fragments arranged by size:
2628 1878 1104 977
AgeI A'CCGG_T
Cuts at: 1369 4975 1369
Size: 3606 2981
AleI CACnn'nnGTG
Cuts at: 1624 3370 1624
Size: 1746 4841
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1388 2008 2092 1388
Size: 620 84 5883
Fragments arranged by size:
5883 620 84
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1942 1974 1942
Size: 32 6555
AlwNI CAG_nnn'CTG
Cuts at: 2031 2460 3892 5459 2031
Size: 429 1432 1567 3159
Fragments arranged by size:
3159 1567 1432 429
ApaLI G'TGCA_C
Cuts at: 2818 5357 2818
Size: 2539 4048
ApoI r'AATT_y
Cuts at: 3591 5940 6196 3591
Size: 2349 256 3982
Fragments arranged by size:
3982 2349 256
AseI AT'TA_AT
Cuts at: 20 2897 20
Size: 2877 3710
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6587
AvaI C'yCGr_G
Cuts at: 220 254 286 679 904 1954 2483 2949 2991
Size: 34 32 393 225 1050 529 466 42
Cuts at: 2991 3403 3427 3475 3607 220
Size: 412 24 48 132 3200
Fragments arranged by size:
3200 1050 529 466 412 393 225 132
48 42 34 32 24
AvrII C'CTAG_G
Cuts at: 3237 3237
Size: 6587
BamHI G'GATC_C
Cuts at: 1976 3612 1976
Size: 1636 4951
BanI G'GyrC_C
Cuts at: 126 176 420 705 860 1372 1796 2100 3215
Size: 50 244 285 155 512 424 304 1115
Cuts at: 3215 3603 4017 4265 4300 126
Size: 388 414 248 35 2413
Fragments arranged by size:
2413 1115 512 424 414 388 304 285
248 244 155 50 35
BanII G_rGCy'C
Cuts at: 254 293 926 1208 1640 1888 1955 2149 2594
Size: 39 633 282 432 248 67 194 445
Cuts at: 2594 2720 3308 3404 3428 3601 4631 254
Size: 126 588 96 24 173 1030 2210
Fragments arranged by size:
2210 1030 633 588 445 432 282 248
194 173 126 96 67 39 24
BbeI G_GCGC'C
Cuts at: 130 180 709 864 3219 4021 4269 130
Size: 50 529 155 2355 802 248 2448
Fragments arranged by size:
2448 2355 802 529 248 155 50
BbsI GAAGACnn'nnnn_
Cuts at: 2917 2917
Size: 6587
BbvCI CC'TCA_GC
Cuts at: 2243 2243
Size: 6587
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1402 1436 2622 2656 1402
Size: 34 1186 34 5333
Fragments arranged by size:
5333 1186 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2337 2424 4485 5246 6587 2337
Size: 87 2061 761 1341 2337
Fragments arranged by size:
2337 2061 1341 761 87
BclI T'GATC_A
Cuts at: 4107 4107
Size: 6587
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 6587
BglII A'GATC_T
Cuts at: 1901 1916 4102 6139 1901
Size: 15 2186 2037 2349
Fragments arranged by size:
2349 2186 2037 15
BlpI GC'TnA_GC
Cuts at: 136 1091 3699 136
Size: 955 2608 3024
Fragments arranged by size:
3024 2608 955
Bme1580I G_kGCm'C
Cuts at: 169 425 1344 1852 2105 2822 3201 4212 4305
Size: 256 919 508 253 717 379 1011 93
Cuts at: 4305 5361 169
Size: 1056 1395
Fragments arranged by size:
1395 1056 1011 919 717 508 379 256
253 93
BmgBI CAC'GTC
Cuts at: 267 267
Size: 6587
BmrI ACTGGGnnnn_n'
Cuts at: 1425 1954 2172 3435 3799 3880 4215 6359 1425
Size: 529 218 1263 364 81 335 2144 1653
Fragments arranged by size:
2144 1653 1263 529 364 335 218 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4954 4954
Size: 6587
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 664 1482 1732 2510 2734 191
Size: 473 818 250 778 224 4044
Fragments arranged by size:
4044 818 778 473 250 224
Bpu10I CC'TnA_GC
Cuts at: 248 773 2243 3289 248
Size: 525 1470 1046 3546
Fragments arranged by size:
3546 1470 1046 525
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1882 3763 4642 5134 5432 1882
Size: 1881 879 492 298 3037
Fragments arranged by size:
3037 1881 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 736 781 1156 2511 3352 3430 3454 3547 6291
Size: 45 375 1355 841 78 24 93 2744
Cuts at: 6291 736
Size: 1032
Fragments arranged by size:
2744 1355 1032 841 375 93 78 45
24
BsaAI yAC'GTr
Cuts at: 1026 2062 3940 1026
Size: 1036 1878 3673
Fragments arranged by size:
3673 1878 1036
BsaBI GATnn'nnATC
Cuts at: 6 4101 6138 6
Size: 4095 2037 455
Fragments arranged by size:
4095 2037 455
BsaHI Gr'CG_yC
Cuts at: 127 177 706 861 1240 1475 3156 3171 3216
Size: 50 529 155 379 235 1681 15 45
Cuts at: 3216 4018 4266 4968 6464 127
Size: 802 248 702 1496 250
Fragments arranged by size:
1681 1496 802 702 529 379 250 248
235 155 50 45 15
BsaWI w'CCGG_w
Cuts at: 366 1369 2543 3776 4297 4975 5249 5396 5807
Size: 1003 1174 1233 521 678 274 147 411
Cuts at: 5807 366
Size: 1146
Fragments arranged by size:
1233 1174 1146 1003 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1219 1249 1942 1972 2491 2521 3240 3270 3391
Size: 30 693 30 519 30 719 30 121
Cuts at: 3391 3415 3421 3439 3445 3469 1219
Size: 24 6 18 6 24 4337
Fragments arranged by size:
4337 719 693 519 121 30 30 30
30 24 24 18 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 226 697 754 796 799 912 946 973 1058
Size: 471 57 42 3 113 34 27 85
Cuts at: 1058 1132 1174 1234 1243 1246 1305 1858 2200
Size: 74 42 60 9 3 59 553 342
Cuts at: 2200 2260 3505 3508 3511 3514 3523 3526 226
Size: 60 1245 3 3 3 9 3 3287
Fragments arranged by size:
3287 1245 553 471 342 113 85 74
60 60 59 57 42 42 34 27
9 9 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 667 948 1719 1972 1995 2169 2379 3338 3464
Size: 281 771 253 23 174 210 959 126
Cuts at: 3464 4012 4770 5347 5843 667
Size: 548 758 577 496 1411
Fragments arranged by size:
1411 959 771 758 577 548 496 281
253 210 174 126 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2098 2098
Size: 6587
BsiEI CG_ry'CG
Cuts at: 104 116 353 1353 4129 4175 5383 104
Size: 12 237 1000 2776 46 1208 1308
Fragments arranged by size:
2776 1308 1208 1000 237 46 12
BsiHKAI G_wGCw'C
Cuts at: 767 1640 1729 1888 2234 2720 2822 3295 3601
Size: 873 89 159 346 486 102 473 306
Cuts at: 3601 4379 4569 5361 767
Size: 778 190 792 1993
Fragments arranged by size:
1993 873 792 778 486 473 346 306
190 159 102 89
BsmI GAATG_Cn'
Cuts at: 3532 3532
Size: 6587
Bsp1286I G_dGCh'C
Cuts at: 169 254 293 425 767 926 1208 1344 1640
Size: 85 39 132 342 159 282 136 296
Cuts at: 1640 1729 1852 1888 1955 2105 2149 2234 2594
Size: 89 123 36 67 150 44 85 360
Cuts at: 2594 2720 2822 3201 3295 3308 3404 3428 3601
Size: 126 102 379 94 13 96 24 173
Cuts at: 3601 4212 4305 4379 4569 4631 5361 169
Size: 611 93 74 190 62 730 1395
Fragments arranged by size:
1395 730 611 379 360 342 296 282
190 173 159 150 136 132 126 123
102 96 94 93 89 85 85 74
67 62 44 39 36 24 13
BspEI T'CCGG_A
Cuts at: 366 5807 366
Size: 5441 1146
BspHI T'CATG_A
Cuts at: 1826 2751 5763 1826
Size: 925 3012 2650
Fragments arranged by size:
3012 2650 925
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2705 2805 3013 3637 3851 4153 4534 2705
Size: 100 208 624 214 302 381 4758
Fragments arranged by size:
4758 624 381 302 214 208 100
BsrBI CCG'CTC
Cuts at: 50 909 2406 4879 4933 6417 6582 50
Size: 859 1497 2473 54 1484 165 55
Fragments arranged by size:
2473 1497 1484 859 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1551 2348 2864 3015 4499 1551
Size: 797 516 151 1484 3639
Fragments arranged by size:
3639 1484 797 516 151
BsrFI r'CCGG_y
Cuts at: 1352 1369 1647 2626 3104 4585 4766 4975 1352
Size: 17 278 979 478 1481 181 209 2964
Fragments arranged by size:
2964 1481 979 478 278 209 181 17
BsrGI T'GTAC_A
Cuts at: 1568 1568
Size: 6587
BssSI C'ACGA_G
Cuts at: 1889 4858 5216 1889
Size: 2969 358 3260
Fragments arranged by size:
3260 2969 358
BstAPI GCAn_nnn'nTGC
Cuts at: 189 1700 3964 189
Size: 1511 2264 2812
Fragments arranged by size:
2812 2264 1511
BstBI TT'CG_AA
Cuts at: 4948 4948
Size: 6587
BstXI CCAn_nnnn'nTGG
Cuts at: 1275 1685 2712 1275
Size: 410 1027 5150
Fragments arranged by size:
5150 1027 410
BstYI r'GATC_y
Cuts at: 1 1304 1901 1916 1976 2217 3612 4079 4102
Size: 1303 597 15 60 241 1395 467 23
Cuts at: 4102 4437 4683 5695 5781 5793 6139 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1395 1303 1012 597 467 449 346 335
246 241 86 60 23 15 12
Bsu36I CC'TnA_GG
Cuts at: 515 929 515
Size: 414 6173
BtgI C'CryG_G
Cuts at: 107 298 1329 1910 1980 2321 2330 2471 2598
Size: 191 1031 581 70 341 9 141 127
Cuts at: 2598 2823 107
Size: 225 3871
Fragments arranged by size:
3871 1031 581 341 225 191 141 127
70 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2063 3113 4537 4718 4881 2063
Size: 1050 1424 181 163 3769
Fragments arranged by size:
3769 1424 1050 181 163
BtsI GCAGTG_nn'
Cuts at: 652 1184 1967 3830 652
Size: 532 783 1863 3409
Fragments arranged by size:
3409 1863 783 532
DraI TTT'AAA
Cuts at: 3586 3586
Size: 6587
DraIII CAC_nnn'GTG
Cuts at: 2100 2907 3038 3252 2100
Size: 807 131 214 5435
Fragments arranged by size:
5435 807 214 131
DrdI GACnn_nn'nnGTC
Cuts at: 4293 5151 4293
Size: 858 5729
EaeI y'GGCC_r
Cuts at: 113 340 350 649 916 1350 1772 2462 3089
Size: 227 10 299 267 434 422 690 627
Cuts at: 3089 3525 4172 4346 4737 4764 6109 113
Size: 436 647 174 391 27 1345 591
Fragments arranged by size:
1345 690 647 627 591 436 434 422
391 299 267 227 174 27 10
EagI C'GGCC_G
Cuts at: 113 350 1350 4172 113
Size: 237 1000 2822 2528
Fragments arranged by size:
2822 2528 1000 237
EarI CTCTTCn'nnn_
Cuts at: 604 1103 3482 3500 4610 4820 604
Size: 499 2379 18 1110 210 2371
Fragments arranged by size:
2379 2371 1110 499 210 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1061 4795 5105 5251 6397 1061
Size: 3734 310 146 1146 1251
Fragments arranged by size:
3734 1251 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1638 1886 2718 3599 1638
Size: 248 832 881 4626
Fragments arranged by size:
4626 881 832 248
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 664 1144 1482 1524 1732 2510 2673 2734
Size: 473 480 338 42 208 778 163 61
Cuts at: 2734 4412 4844 5591 5859 191
Size: 1678 432 747 268 919
Fragments arranged by size:
1678 919 778 747 480 473 432 338
268 208 163 61 42
EcoNI CCTnn'n_nnAGG
Cuts at: 2077 2077
Size: 6587
EcoO109I rG'GnC_Cy
Cuts at: 273 412 443 614 939 1427 2259 2271 3241
Size: 139 31 171 325 488 832 12 970
Cuts at: 3241 3312 3726 273
Size: 71 414 3134
Fragments arranged by size:
3134 970 832 488 414 325 171 139
71 31 12
EcoRI G'AATT_C
Cuts at: 3591 3591
Size: 6587
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 450 2388 4606 450
Size: 1938 2218 2431
Fragments arranged by size:
2431 2218 1938
FspI TGC'GCA
Cuts at: 2068 4368 2068
Size: 2300 4287
HaeII r_GCGC'y
Cuts at: 130 180 709 864 1098 2170 2797 2999 3219
Size: 50 529 155 234 1072 627 202 220
Cuts at: 3219 4021 4269 5291 6270 130
Size: 802 248 1022 979 447
Fragments arranged by size:
1072 1022 979 802 627 529 447 248
234 220 202 155 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 957 989 2209 2241 2491 2523 4429 4459 4461
Size: 32 1220 32 250 32 1906 30 2
Cuts at: 4461 4491 957
Size: 30 3053
Fragments arranged by size:
3053 1906 1220 250 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2646 3015 3626 2646
Size: 369 611 5607
Fragments arranged by size:
5607 611 369
KasI G'GCGC_C
Cuts at: 126 176 705 860 3215 4017 4265 126
Size: 50 529 155 2355 802 248 2448
Fragments arranged by size:
2448 2355 802 529 248 155 50
KpnI G_GTAC'C
Cuts at: 3607 3607
Size: 6587
MluI A'CGCG_T
Cuts at: 6020 6020
Size: 6587
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1205 3086 3329 3425 5258 5442 1205
Size: 1881 243 96 1833 184 2350
Fragments arranged by size:
2350 1881 1833 243 184 96
MscI TGG'CCA
Cuts at: 651 918 1774 3091 3527 4348 651
Size: 267 856 1317 436 821 2890
Fragments arranged by size:
2890 1317 856 821 436 267
MslI CAynn'nnrTG
Cuts at: 1273 1624 1831 2756 2806 2884 3091 3370 4576
Size: 351 207 925 50 78 207 279 1206
Cuts at: 4576 4703 1273
Size: 127 3157
Fragments arranged by size:
3157 1206 925 351 279 207 207 127
78 50
MspA1I CmG'CkG
Cuts at: 140 300 999 2457 2600 2825 3334 3468 3698
Size: 160 699 1458 143 225 509 134 230
Cuts at: 3698 4012 4372 5385 5630 140
Size: 314 360 1013 245 1097
Fragments arranged by size:
1458 1097 1013 699 509 360 314 245
230 225 160 143 134
NaeI GCC'GGC
Cuts at: 1354 4768 1354
Size: 3414 3173
NarI GG'CG_CC
Cuts at: 127 177 706 861 3216 4018 4266 127
Size: 50 529 155 2355 802 248 2448
Fragments arranged by size:
2448 2355 802 529 248 155 50
NcoI C'CATG_G
Cuts at: 107 1910 1980 2471 107
Size: 1803 70 491 4223
Fragments arranged by size:
4223 1803 491 70
NgoMIV G'CCGG_C
Cuts at: 1352 4766 1352
Size: 3414 3173
NspI r_CATG'y
Cuts at: 1365 1707 1818 3640 4140 5047 1365
Size: 342 111 1822 500 907 2905
Fragments arranged by size:
2905 1822 907 500 342 111
PasI CC'CwG_GG
Cuts at: 1485 1930 3308 1485
Size: 445 1378 4764
Fragments arranged by size:
4764 1378 445
PciI A'CATG_T
Cuts at: 5043 5043
Size: 6587
PflMI CCAn_nnn'nTGG
Cuts at: 1335 1335
Size: 6587
PfoI T'CCnGG_A
Cuts at: 732 2213 3077 732
Size: 1481 864 4242
Fragments arranged by size:
4242 1481 864
PmeI GTTT'AAAC
Cuts at: 3586 3586
Size: 6587
PmlI CAC'GTG
Cuts at: 2062 2062
Size: 6587
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1942 1974 5726 5758 1942
Size: 32 3752 32 2771
Fragments arranged by size:
3752 2771 32 32
PpuMI rG'GwC_Cy
Cuts at: 443 1427 2259 2271 443
Size: 984 832 12 4759
Fragments arranged by size:
4759 984 832 12
PspXI vC'TCGA_Gb
Cuts at: 220 220
Size: 6587
PstI C_TGCA'G
Cuts at: 3164 3634 4319 3164
Size: 470 685 5432
Fragments arranged by size:
5432 685 470
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6587
PvuII CAG'CTG
Cuts at: 140 2457 3468 4012 4372 140
Size: 2317 1011 544 360 2355
Fragments arranged by size:
2355 2317 1011 544 360
RsrII CG'GwC_CG
Cuts at: 2669 4782 2669
Size: 2113 4474
SacI G_AGCT'C
Cuts at: 1640 1888 2720 3601 1640
Size: 248 832 881 4626
Fragments arranged by size:
4626 881 832 248
SacII CC_GC'GG
Cuts at: 301 2601 2826 301
Size: 2300 225 4062
Fragments arranged by size:
4062 2300 225
SalI G'TCGA_C
Cuts at: 3624 3624
Size: 6587
SanDI GG'GwC_CC
Cuts at: 443 2259 2271 443
Size: 1816 12 4759
Fragments arranged by size:
4759 1816 12
SapI GCTCTTCn'nnn_
Cuts at: 4610 4820 4610
Size: 210 6377
SbfI CC_TGCA'GG
Cuts at: 3634 3634
Size: 6587
ScaI AGT'ACT
Cuts at: 6104 6104
Size: 6587
SexAI A'CCwGG_T
Cuts at: 2096 2096
Size: 6587
SfcI C'TryA_G
Cuts at: 33 1743 3160 3630 4315 5308 5499 33
Size: 1710 1417 470 685 993 191 1121
Fragments arranged by size:
1710 1417 1121 993 685 470 191
SfoI GGC'GCC
Cuts at: 128 178 707 862 3217 4019 4267 128
Size: 50 529 155 2355 802 248 2448
Fragments arranged by size:
2448 2355 802 529 248 155 50
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6587
SgrAI Cr'CCGG_yG
Cuts at: 2626 2626
Size: 6587
SmaI CCC'GGG
Cuts at: 256 2485 2951 3609 256
Size: 2229 466 658 3234
Fragments arranged by size:
3234 2229 658 466
SmlI C'TyrA_G
Cuts at: 220 1897 3742 4657 5149 5411 220
Size: 1677 1845 915 492 262 1396
Fragments arranged by size:
1845 1677 1396 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6587
SphI G_CATG'C
Cuts at: 1707 3640 4140 1707
Size: 1933 500 4154
Fragments arranged by size:
4154 1933 500
StuI AGG'CCT
Cuts at: 1895 1895
Size: 6587
StyI C'CwwG_G
Cuts at: 107 416 425 434 482 500 533 548 581
Size: 309 9 9 48 18 33 15 33
Cuts at: 581 1154 1910 1980 2471 3237 3416 3721 4075
Size: 573 756 70 491 766 179 305 354
Cuts at: 4075 107
Size: 2619
Fragments arranged by size:
2619 766 756 573 491 354 309 305
179 70 48 33 33 18 15 9
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 211 495 561 594 805 1180 1498 1551 2602
Size: 284 66 33 211 375 318 53 1051
Cuts at: 2602 3406 4170 4303 4762 4971 211
Size: 804 764 133 459 209 1827
Fragments arranged by size:
1827 1051 804 764 459 375 318 284
211 209 133 66 53 33
TatI w'GTAC_w
Cuts at: 851 1568 6102 851
Size: 717 4534 1336
Fragments arranged by size:
4534 1336 717
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1219 1251 2046 2078 3240 3272 6104 6136 1219
Size: 32 795 32 1162 32 2832 32 1670
Fragments arranged by size:
2832 1670 1162 795 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2503 4384 2503
Size: 1881 4706
XbaI T'CTAG_A
Cuts at: 3618 3618
Size: 6587
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 432 1530 1782 4082 432
Size: 1098 252 2300 2937
Fragments arranged by size:
2937 2300 1098 252
XhoI C'TCGA_G
Cuts at: 220 220
Size: 6587
XmaI C'CCGG_G
Cuts at: 254 2483 2949 3607 254
Size: 2229 466 658 3234
Fragments arranged by size:
3234 2229 658 466
XmnI GAAnn'nnTTC
Cuts at: 2371 2371
Size: 6587
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI ApaI AscI BaeI BfrBI
BmtI BsiWI BsmBI BssHII BstEII BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PshAI PsiI PspOMI PsrI
SfiI SnaBI SrfI SspI SwaI ZraI