Restriction map for
pF1KB5095
(Circular) (Six-base) MAPSORT of: pF1KB5095.seq Check: 4577 from: 1 to: 6035
pF1KB5095 6035 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 1628 3073 1628
Size: 1445 4590
Acc65I G'GTAC_C
Cuts at: 238 3051 238
Size: 2813 3222
AclI AA'CG_TT
Cuts at: 5875 5875
Size: 6035
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2111 2492 3860 4292 4645 5307 2111
Size: 381 1368 432 353 662 2839
Fragments arranged by size:
2839 1368 662 432 381 353
AflIII A'CryG_T
Cuts at: 416 673 3387 5187 5468 416
Size: 257 2714 1800 281 983
Fragments arranged by size:
2714 1800 983 281 257
AleI CACnn'nnGTG
Cuts at: 1010 1697 1894 2011 1010
Size: 687 197 117 5034
Fragments arranged by size:
5034 687 197 117
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 358 818 2092 2158 358
Size: 460 1274 66 4235
Fragments arranged by size:
4235 1274 460 66
AlwNI CAG_nnn'CTG
Cuts at: 2032 2673 2803 3340 4778 2032
Size: 641 130 537 1438 3289
Fragments arranged by size:
3289 1438 641 537 130
ApaLI G'TGCA_C
Cuts at: 1327 4873 1327
Size: 3546 2489
ApoI r'AATT_y
Cuts at: 756 1478 1486 2257 3039 5388 5644 756
Size: 722 8 771 782 2349 256 1147
Fragments arranged by size:
2349 1147 782 771 722 256 8
AseI AT'TA_AT
Cuts at: 20 1951 20
Size: 1931 4104
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6035
AvaI C'yCGr_G
Cuts at: 389 2691 2815 3055 389
Size: 2302 124 240 3369
Fragments arranged by size:
3369 2302 240 124
BamHI G'GATC_C
Cuts at: 1923 2022 2228 3060 1923
Size: 99 206 832 4898
Fragments arranged by size:
4898 832 206 99
BanI G'GyrC_C
Cuts at: 238 1564 3051 3465 3713 3748 238
Size: 1326 1487 414 248 35 2525
Fragments arranged by size:
2525 1487 1326 414 248 35
BanII G_rGCy'C
Cuts at: 895 3049 4079 895
Size: 2154 1030 2851
Fragments arranged by size:
2851 2154 1030
BbeI G_GCGC'C
Cuts at: 3469 3717 3469
Size: 248 5787
BbsI GAAGACnn'nnnn_
Cuts at: 1781 2117 1781
Size: 336 5699
BbvCI CC'TCA_GC
Cuts at: 896 896
Size: 6035
BciVI GTATCCnnnnn_n'
Cuts at: 396 841 3933 4989 6035 396
Size: 445 3092 1056 1046 396
Fragments arranged by size:
3092 1056 1046 445 396
BclI T'GATC_A
Cuts at: 2675 3555 2675
Size: 880 5155
BfrBI ATG'CAT
Cuts at: 795 1182 795
Size: 387 5648
BglI GCCn_nnn'nGGC
Cuts at: 2903 2903
Size: 6035
BglII A'GATC_T
Cuts at: 3550 5587 3550
Size: 2037 3998
BlpI GC'TnA_GC
Cuts at: 2932 3147 2932
Size: 215 5820
Bme1580I G_kGCm'C
Cuts at: 1331 3660 3753 4877 1331
Size: 2329 93 1124 2489
Fragments arranged by size:
2489 2329 1124 93
BmgBI CAC'GTC
Cuts at: 676 1019 1896 676
Size: 343 877 4815
Fragments arranged by size:
4815 877 343
BmrI ACTGGGnnnn_n'
Cuts at: 2848 3247 3328 3663 5807 2848
Size: 399 81 335 2144 3076
Fragments arranged by size:
3076 2144 399 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 542 2156 2706 4402 542
Size: 1614 550 1696 2175
Fragments arranged by size:
2175 1696 1614 550
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1094 1643 1801 2054 1094
Size: 549 158 253 5075
Fragments arranged by size:
5075 549 253 158
Bpu10I CC'TnA_GC
Cuts at: 896 896
Size: 6035
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1530 1878 3211 4090 4804 5102 1530
Size: 348 1333 879 714 298 2463
Fragments arranged by size:
2463 1333 879 714 348 298
BsaI GGTCTCn'nnnn_
Cuts at: 5739 5739
Size: 6035
BsaAI yAC'GTr
Cuts at: 3388 3388
Size: 6035
BsaBI GATnn'nnATC
Cuts at: 6 3549 5586 6
Size: 3543 2037 455
Fragments arranged by size:
3543 2037 455
BsaHI Gr'CG_yC
Cuts at: 3466 3714 4416 5912 3466
Size: 248 702 1496 3589
Fragments arranged by size:
3589 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2733 3224 3745 4423 4834 4981 5255 2733
Size: 491 521 678 411 147 274 3513
Fragments arranged by size:
3513 678 521 491 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 734 764 2687 2717 734
Size: 30 1923 30 4052
Fragments arranged by size:
4052 1923 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 584 2628 3001 3015 584
Size: 2044 373 14 3604
Fragments arranged by size:
3604 2044 373 14
BseYI C'CCAG_C
Cuts at: 735 3460 4218 4883 5291 735
Size: 2725 758 665 408 1479
Fragments arranged by size:
2725 1479 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1457 2098 1457
Size: 641 5394
BsiEI CG_ry'CG
Cuts at: 104 3577 3623 4853 104
Size: 3473 46 1230 1286
Fragments arranged by size:
3473 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1331 3049 3827 4017 4877 1331
Size: 1718 778 190 860 2489
Fragments arranged by size:
2489 1718 860 778 190
BsmI GAATG_Cn'
Cuts at: 2277 2277
Size: 6035
BsmBI CGTCTCn'nnnn_
Cuts at: 2812 2812
Size: 6035
Bsp1286I G_dGCh'C
Cuts at: 895 1331 3049 3660 3753 3827 4017 4079 4877
Size: 436 1718 611 93 74 190 62 798
Cuts at: 4877 895
Size: 2053
Fragments arranged by size:
2053 1718 798 611 436 190 93 74
62
BspHI T'CATG_A
Cuts at: 1085 2454 4467 1085
Size: 1369 2013 2653
Fragments arranged by size:
2653 2013 1369
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3085 3299 3601 3982 3085
Size: 214 302 381 5138
Fragments arranged by size:
5138 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4327 4381 5865 6030 50
Size: 4277 54 1484 165 55
Fragments arranged by size:
4277 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2753 3947 2753
Size: 1194 4841
BsrFI r'CCGG_y
Cuts at: 2927 4033 4214 2927
Size: 1106 181 4748
Fragments arranged by size:
4748 1106 181
BsrGI T'GTAC_A
Cuts at: 484 2373 2486 484
Size: 1889 113 4033
Fragments arranged by size:
4033 1889 113
BssSI C'ACGA_G
Cuts at: 4306 5014 4306
Size: 708 5327
BstAPI GCAn_nnn'nTGC
Cuts at: 2655 3412 2655
Size: 757 5278
BstBI TT'CG_AA
Cuts at: 4396 4396
Size: 6035
BstEII G'GTnAC_C
Cuts at: 949 1699 949
Size: 750 5285
BstXI CCAn_nnnn'nTGG
Cuts at: 1651 1825 2013 1651
Size: 174 188 5673
Fragments arranged by size:
5673 188 174
BstYI r'GATC_y
Cuts at: 1 1407 1794 1923 2022 2228 3060 3527 3550
Size: 1406 387 129 99 206 832 467 23
Cuts at: 3550 3885 4131 4437 4449 4535 5587 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1406 1052 832 467 449 387 335 306
246 206 129 99 86 23 12
BstZ17I GTA'TAC
Cuts at: 1629 1629
Size: 6035
BtgI C'CryG_G
Cuts at: 107 107
Size: 6035
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3985 4166 4329 3985
Size: 181 163 5691
Fragments arranged by size:
5691 181 163
BtsI GCAGTG_nn'
Cuts at: 1986 2342 2952 3278 1986
Size: 356 610 326 4743
Fragments arranged by size:
4743 610 356 326
ClaI AT'CG_AT
Cuts at: 2550 2550
Size: 6035
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 990 1025 1838 1873 990
Size: 35 813 35 5152
Fragments arranged by size:
5152 813 35 35
DraI TTT'AAA
Cuts at: 201 2241 3034 201
Size: 2040 793 3202
Fragments arranged by size:
3202 2040 793
DraIII CAC_nnn'GTG
Cuts at: 1558 2092 2111 2516 1558
Size: 534 19 405 5077
Fragments arranged by size:
5077 534 405 19
DrdI GACnn_nn'nnGTC
Cuts at: 3741 5085 3741
Size: 1344 4691
EaeI y'GGCC_r
Cuts at: 114 2750 3620 3794 4185 4212 5557 114
Size: 2636 870 174 391 27 1345 592
Fragments arranged by size:
2636 1345 870 592 391 174 27
EagI C'GGCC_G
Cuts at: 3620 3620
Size: 6035
EarI CTCTTCn'nnn_
Cuts at: 1598 2114 2826 2978 4058 4268 1598
Size: 516 712 152 1080 210 3365
Fragments arranged by size:
3365 1080 712 516 210 152
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4243 4985 5131 5845 4243
Size: 742 146 714 4433
Fragments arranged by size:
4433 742 714 146
EcoICRI GAG'CTC
Cuts at: 3047 3047
Size: 6035
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1094 1643 1801 2054 2111 2492 3860 4292 4645
Size: 549 158 253 57 381 1368 432 353
Cuts at: 4645 5307 1094
Size: 662 1822
Fragments arranged by size:
1822 1368 662 549 432 381 353 253
158 57
EcoNI CCTnn'n_nnAGG
Cuts at: 1559 2144 2375 1559
Size: 585 231 5219
Fragments arranged by size:
5219 585 231
EcoO109I rG'GnC_Cy
Cuts at: 1942 2786 3174 1942
Size: 844 388 4803
Fragments arranged by size:
4803 844 388
EcoRI G'AATT_C
Cuts at: 756 1478 3039 756
Size: 722 1561 3752
Fragments arranged by size:
3752 1561 722
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2544 4054 2544
Size: 1510 4525
FspI TGC'GCA
Cuts at: 2434 3816 2434
Size: 1382 4653
HaeII r_GCGC'y
Cuts at: 3469 3717 4947 5718 3469
Size: 248 1230 771 3786
Fragments arranged by size:
3786 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1705 1737 3877 3907 3909 3939 1705
Size: 32 2140 30 2 30 3801
Fragments arranged by size:
3801 2140 32 30 30 2
HincII GTy'rAC
Cuts at: 723 2002 3074 723
Size: 1279 1072 3684
Fragments arranged by size:
3684 1279 1072
KasI G'GCGC_C
Cuts at: 3465 3713 3465
Size: 248 5787
KpnI G_GTAC'C
Cuts at: 242 3055 242
Size: 2813 3222
MfeI C'AATT_G
Cuts at: 1766 1766
Size: 6035
MluI A'CGCG_T
Cuts at: 5468 5468
Size: 6035
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 534 546 2206 4794 4978 534
Size: 12 1660 2588 184 1591
Fragments arranged by size:
2588 1660 1591 184 12
MscI TGG'CCA
Cuts at: 2752 3796 2752
Size: 1044 4991
MslI CAynn'nnrTG
Cuts at: 340 1010 1697 1894 2011 4024 4151 340
Size: 670 687 197 117 2013 127 2224
Fragments arranged by size:
2224 2013 687 670 197 127 117
MspA1I CmG'CkG
Cuts at: 158 900 3146 3460 3820 4604 4849 158
Size: 742 2246 314 360 784 245 1344
Fragments arranged by size:
2246 1344 784 742 360 314 245
NaeI GCC'GGC
Cuts at: 4216 4216
Size: 6035
NarI GG'CG_CC
Cuts at: 3466 3714 3466
Size: 248 5787
NcoI C'CATG_G
Cuts at: 107 107
Size: 6035
NgoMIV G'CCGG_C
Cuts at: 4214 4214
Size: 6035
NsiI A_TGCA'T
Cuts at: 797 1184 797
Size: 387 5648
NspI r_CATG'y
Cuts at: 297 339 420 705 799 1075 1971 3088 3588
Size: 42 81 285 94 276 896 1117 500
Cuts at: 3588 5191 297
Size: 1603 1141
Fragments arranged by size:
1603 1141 1117 896 500 285 276 94
81 42
PciI A'CATG_T
Cuts at: 416 5187 416
Size: 4771 1264
PflMI CCAn_nnn'nTGG
Cuts at: 2032 2032
Size: 6035
PfoI T'CCnGG_A
Cuts at: 2579 2579
Size: 6035
PmeI GTTT'AAAC
Cuts at: 3034 3034
Size: 6035
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2798 2830 4481 4513 2798
Size: 32 1651 32 4320
Fragments arranged by size:
4320 1651 32 32
PsiI TTA'TAA
Cuts at: 779 779
Size: 6035
PspXI vC'TCGA_Gb
Cuts at: 389 389
Size: 6035
PstI C_TGCA'G
Cuts at: 1812 3082 3767 1812
Size: 1270 685 4080
Fragments arranged by size:
4080 1270 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6035
PvuII CAG'CTG
Cuts at: 158 900 3460 3820 158
Size: 742 2560 360 2373
Fragments arranged by size:
2560 2373 742 360
RsrII CG'GwC_CG
Cuts at: 4230 4230
Size: 6035
SacI G_AGCT'C
Cuts at: 3049 3049
Size: 6035
SalI G'TCGA_C
Cuts at: 3072 3072
Size: 6035
SapI GCTCTTCn'nnn_
Cuts at: 4058 4268 4058
Size: 210 5825
SbfI CC_TGCA'GG
Cuts at: 3082 3082
Size: 6035
ScaI AGT'ACT
Cuts at: 1814 2837 5552 1814
Size: 1023 2715 2297
Fragments arranged by size:
2715 2297 1023
SexAI A'CCwGG_T
Cuts at: 1370 1554 1679 1370
Size: 184 125 5726
Fragments arranged by size:
5726 184 125
SfcI C'TryA_G
Cuts at: 33 598 807 1228 1343 1808 2907 3078 3763
Size: 565 209 421 115 465 1099 171 685
Cuts at: 3763 4731 4922 33
Size: 968 191 1146
Fragments arranged by size:
1146 1099 968 685 565 465 421 209
191 171 115
SfoI GGC'GCC
Cuts at: 3467 3715 3467
Size: 248 5787
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6035
SmaI CCC'GGG
Cuts at: 3057 3057
Size: 6035
SmlI C'TyrA_G
Cuts at: 389 1509 1857 2815 3190 4105 4819 5081 389
Size: 1120 348 958 375 915 714 262 1343
Fragments arranged by size:
1343 1120 958 915 714 375 348 262
SpeI A'CTAG_T
Cuts at: 67 1497 67
Size: 1430 4605
SphI G_CATG'C
Cuts at: 297 1075 1971 3088 3588 297
Size: 778 896 1117 500 2744
Fragments arranged by size:
2744 1117 896 778 500
SspI AAT'ATT
Cuts at: 501 501
Size: 6035
StyI C'CwwG_G
Cuts at: 107 2877 3169 3523 107
Size: 2770 292 354 2619
Fragments arranged by size:
2770 2619 354 292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3618 3751 4210 4419 3618
Size: 133 459 209 5234
Fragments arranged by size:
5234 459 209 133
TatI w'GTAC_w
Cuts at: 484 594 1812 2373 2486 2835 5550 484
Size: 110 1218 561 113 349 2715 969
Fragments arranged by size:
2715 1218 969 561 349 113 110
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 734 766 5552 5584 734
Size: 32 4786 32 1185
Fragments arranged by size:
4786 1185 32 32
Tth111I GACn'n_nGTC
Cuts at: 1705 3832 1705
Size: 2127 3908
XbaI T'CTAG_A
Cuts at: 1662 3066 1662
Size: 1404 4631
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1711 3530 1711
Size: 1819 4216
XhoI C'TCGA_G
Cuts at: 389 2815 389
Size: 2426 3609
XmaI C'CCGG_G
Cuts at: 3055 3055
Size: 6035
XmnI GAAnn'nnTTC
Cuts at: 1993 1993
Size: 6035
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PflMI PfoI PmeI PpiI PsiI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI ApaI
AscI AvrII BaeI BcgI BmtI BsiWI BspEI BssHII
Bsu36I EcoRV FseI FspAI HindIII HpaI NdeI NheI
NotI NruI PacI PasI PmlI PpuMI PshAI PspOMI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI StuI
SwaI ZraI