Restriction map for
pF1KB5093
(Circular) (Six-base) MAPSORT of: pF1KB5093.seq Check: 1934 from: 1 to: 5966
pF1KB5093 5966 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 3004 3004
Size: 5966
Acc65I G'GTAC_C
Cuts at: 2982 2982
Size: 5966
AclI AA'CG_TT
Cuts at: 2233 5806 2233
Size: 3573 2393
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2572 3791 4223 4576 5238 2572
Size: 1219 432 353 662 3300
Fragments arranged by size:
3300 1219 662 432 353
AfeI AGC'GCT
Cuts at: 290 290
Size: 5966
AflII C'TTAA_G
Cuts at: 927 927
Size: 5966
AflIII A'CryG_T
Cuts at: 2559 3318 5118 5399 2559
Size: 759 1800 281 3126
Fragments arranged by size:
3126 1800 759 281
AlwNI CAG_nnn'CTG
Cuts at: 651 1060 1958 3271 4709 651
Size: 409 898 1313 1438 1908
Fragments arranged by size:
1908 1438 1313 898 409
ApaI G_GGCC'C
Cuts at: 118 170 118
Size: 52 5914
ApaLI G'TGCA_C
Cuts at: 616 4804 616
Size: 4188 1778
ApoI r'AATT_y
Cuts at: 823 1214 1316 1548 1927 2970 5319 5575 823
Size: 391 102 232 379 1043 2349 256 1214
Fragments arranged by size:
2349 1214 1043 391 379 256 232 102
AseI AT'TA_AT
Cuts at: 20 2014 2266 20
Size: 1994 252 3720
Fragments arranged by size:
3720 1994 252
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5966
AvaI C'yCGr_G
Cuts at: 262 541 2986 262
Size: 279 2445 3242
Fragments arranged by size:
3242 2445 279
AvrII C'CTAG_G
Cuts at: 2118 2118
Size: 5966
BamHI G'GATC_C
Cuts at: 2991 2991
Size: 5966
BanI G'GyrC_C
Cuts at: 180 198 609 1255 2982 3396 3644 3679 180
Size: 18 411 646 1727 414 248 35 2467
Fragments arranged by size:
2467 1727 646 414 411 248 35 18
BanII G_rGCy'C
Cuts at: 118 170 272 2980 4010 118
Size: 52 102 2708 1030 2074
Fragments arranged by size:
2708 2074 1030 102 52
BbeI G_GCGC'C
Cuts at: 184 202 3400 3648 184
Size: 18 3198 248 2502
Fragments arranged by size:
3198 2502 248 18
BbsI GAAGACnn'nnnn_
Cuts at: 2543 2543
Size: 5966
BbvCI CC'TCA_GC
Cuts at: 625 625
Size: 5966
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5932
BciVI GTATCCnnnnn_n'
Cuts at: 691 3864 4920 5966 691
Size: 3173 1056 1046 691
Fragments arranged by size:
3173 1056 1046 691
BclI T'GATC_A
Cuts at: 2420 3486 2420
Size: 1066 4900
BfrBI ATG'CAT
Cuts at: 334 334
Size: 5966
BglI GCCn_nnn'nGGC
Cuts at: 267 2110 267
Size: 1843 4123
BglII A'GATC_T
Cuts at: 671 1290 3481 5518 671
Size: 619 2191 2037 1119
Fragments arranged by size:
2191 2037 1119 619
BlpI GC'TnA_GC
Cuts at: 271 3078 271
Size: 2807 3159
Bme1580I G_kGCm'C
Cuts at: 118 170 263 620 3591 3684 4808 118
Size: 52 93 357 2971 93 1124 1276
Fragments arranged by size:
2971 1276 1124 357 93 93 52
BmgBI CAC'GTC
Cuts at: 2560 2560
Size: 5966
BmrI ACTGGGnnnn_n'
Cuts at: 464 1723 3178 3259 3594 5738 464
Size: 1259 1455 81 335 2144 692
Fragments arranged by size:
2144 1455 1259 692 335 81
BmtI G_CTAG'C
Cuts at: 2023 2023
Size: 5966
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2334 4333 2334
Size: 1999 3967
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 808 1558 427
Size: 381 750 4835
Fragments arranged by size:
4835 750 381
Bpu10I CC'TnA_GC
Cuts at: 625 1141 1458 625
Size: 516 317 5133
Fragments arranged by size:
5133 516 317
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 660 3142 4021 4735 5033 660
Size: 2482 879 714 298 1593
Fragments arranged by size:
2482 1593 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 784 1169 5670 784
Size: 385 4501 1080
Fragments arranged by size:
4501 1080 385
BsaAI yAC'GTr
Cuts at: 3319 3319
Size: 5966
BsaBI GATnn'nnATC
Cuts at: 6 917 3480 5517 6
Size: 911 2563 2037 455
Fragments arranged by size:
2563 2037 911 455
BsaHI Gr'CG_yC
Cuts at: 181 199 3397 3645 4347 5843 181
Size: 18 3198 248 702 1496 304
Fragments arranged by size:
3198 1496 702 304 248 18
BsaWI w'CCGG_w
Cuts at: 3155 3676 4354 4765 4912 5186 3155
Size: 521 678 411 147 274 3935
Fragments arranged by size:
3935 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1980 1980
Size: 5966
BseYI C'CCAG_C
Cuts at: 169 1969 3391 4149 4814 5222 169
Size: 1800 1422 758 665 408 913
Fragments arranged by size:
1800 1422 913 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1054 2896 1054
Size: 1842 4124
BsiEI CG_ry'CG
Cuts at: 104 164 222 257 3508 3554 4784 104
Size: 60 58 35 3251 46 1230 1286
Fragments arranged by size:
3251 1286 1230 60 58 46 35
BsiHKAI G_wGCw'C
Cuts at: 620 2980 3758 3948 4808 620
Size: 2360 778 190 860 1778
Fragments arranged by size:
2360 1778 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 118 170 263 272 620 2980 3591 3684 3758
Size: 52 93 9 348 2360 611 93 74
Cuts at: 3758 3948 4010 4808 118
Size: 190 62 798 1276
Fragments arranged by size:
2360 1276 798 611 348 190 93 93
74 62 52 9
BspHI T'CATG_A
Cuts at: 4398 4398
Size: 5966
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1784 3016 3230 3532 3913 1784
Size: 1232 214 302 381 3837
Fragments arranged by size:
3837 1232 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4258 4312 5796 5961 50
Size: 4208 54 1484 165 55
Fragments arranged by size:
4208 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2479 3878 2479
Size: 1399 4567
BsrFI r'CCGG_y
Cuts at: 3964 4145 3964
Size: 181 5785
BsrGI T'GTAC_A
Cuts at: 1672 1807 1672
Size: 135 5831
BssSI C'ACGA_G
Cuts at: 4237 4945 4237
Size: 708 5258
BstAPI GCAn_nnn'nTGC
Cuts at: 507 1958 3343 507
Size: 1451 1385 3130
Fragments arranged by size:
3130 1451 1385
BstBI TT'CG_AA
Cuts at: 4327 4327
Size: 5966
BstYI r'GATC_y
Cuts at: 1 671 1290 2991 3458 3481 3816 4062 4368
Size: 670 619 1701 467 23 335 246 306
Cuts at: 4368 4380 4466 5518 1
Size: 12 86 1052 449
Fragments arranged by size:
1701 1052 670 619 467 449 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 830 1483 2783 830
Size: 653 1300 4013
Fragments arranged by size:
4013 1300 653
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3916 4097 4260 3916
Size: 181 163 5622
Fragments arranged by size:
5622 181 163
BtsI GCAGTG_nn'
Cuts at: 1813 1959 2423 2768 3209 1813
Size: 146 464 345 441 4570
Fragments arranged by size:
4570 464 441 345 146
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2556 2591 2556
Size: 35 5931
DraI TTT'AAA
Cuts at: 1553 2532 2965 1553
Size: 979 433 4554
Fragments arranged by size:
4554 979 433
DraIII CAC_nnn'GTG
Cuts at: 1587 1587
Size: 5966
DrdI GACnn_nn'nnGTC
Cuts at: 3672 5016 3672
Size: 1344 4622
EaeI y'GGCC_r
Cuts at: 161 254 2270 3551 3725 4116 4143 5488 161
Size: 93 2016 1281 174 391 27 1345 639
Fragments arranged by size:
2016 1345 1281 639 391 174 93 27
EagI C'GGCC_G
Cuts at: 161 254 3551 161
Size: 93 3297 2576
Fragments arranged by size:
3297 2576 93
EarI CTCTTCn'nnn_
Cuts at: 353 2065 3989 4199 353
Size: 1712 1924 210 2120
Fragments arranged by size:
2120 1924 1712 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4174 4916 5062 5776 4174
Size: 742 146 714 4364
Fragments arranged by size:
4364 742 714 146
EcoICRI GAG'CTC
Cuts at: 2978 2978
Size: 5966
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 808 1558 2572 3791 4223 4576 5238 427
Size: 381 750 1014 1219 432 353 662 1155
Fragments arranged by size:
1219 1155 1014 750 662 432 381 353
EcoNI CCTnn'n_nnAGG
Cuts at: 1397 1682 1397
Size: 285 5681
EcoO109I rG'GnC_Cy
Cuts at: 2313 3105 2313
Size: 792 5174
EcoRI G'AATT_C
Cuts at: 2970 2970
Size: 5966
EcoRV GAT'ATC
Cuts at: 297 1274 297
Size: 977 4989
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1097 3985 1097
Size: 2888 3078
FspI TGC'GCA
Cuts at: 3747 3747
Size: 5966
HaeII r_GCGC'y
Cuts at: 184 202 278 292 652 3400 3648 4878 5649
Size: 18 76 14 360 2748 248 1230 771
Cuts at: 5649 184
Size: 501
Fragments arranged by size:
2748 1230 771 501 360 248 76 18
14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 645 677 1275 1307 1716 1748 1891 1923 2064
Size: 32 598 32 409 32 143 32 141
Cuts at: 2064 2096 3808 3838 3840 3870 645
Size: 32 1712 30 2 30 2741
Fragments arranged by size:
2741 1712 598 409 143 141 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 760 1479 3005 760
Size: 719 1526 3721
Fragments arranged by size:
3721 1526 719
HindIII A'AGCT_T
Cuts at: 2037 2037
Size: 5966
HpaI GTT'AAC
Cuts at: 1479 1479
Size: 5966
KasI G'GCGC_C
Cuts at: 180 198 3396 3644 180
Size: 18 3198 248 2502
Fragments arranged by size:
3198 2502 248 18
KpnI G_GTAC'C
Cuts at: 2986 2986
Size: 5966
MfeI C'AATT_G
Cuts at: 367 2373 367
Size: 2006 3960
MluI A'CGCG_T
Cuts at: 5399 5399
Size: 5966
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2822 4725 4909 2822
Size: 1903 184 3879
Fragments arranged by size:
3879 1903 184
MscI TGG'CCA
Cuts at: 3727 3727
Size: 5966
MslI CAynn'nnrTG
Cuts at: 944 3955 4082 944
Size: 3011 127 2828
Fragments arranged by size:
3011 2828 127
MspA1I CmG'CkG
Cuts at: 173 209 2747 3077 3391 3751 4535 4780 173
Size: 36 2538 330 314 360 784 245 1359
Fragments arranged by size:
2538 1359 784 360 330 314 245 36
NaeI GCC'GGC
Cuts at: 4147 4147
Size: 5966
NarI GG'CG_CC
Cuts at: 181 199 3397 3645 181
Size: 18 3198 248 2502
Fragments arranged by size:
3198 2502 248 18
NdeI CA'TA_TG
Cuts at: 1503 1503
Size: 5966
NgoMIV G'CCGG_C
Cuts at: 4145 4145
Size: 5966
NheI G'CTAG_C
Cuts at: 2019 2019
Size: 5966
NsiI A_TGCA'T
Cuts at: 336 336
Size: 5966
NspI r_CATG'y
Cuts at: 2353 2649 3019 3519 5122 2353
Size: 296 370 500 1603 3197
Fragments arranged by size:
3197 1603 500 370 296
PasI CC'CwG_GG
Cuts at: 144 144
Size: 5966
PciI A'CATG_T
Cuts at: 5118 5118
Size: 5966
PflMI CCAn_nnn'nTGG
Cuts at: 291 1206 1587 291
Size: 915 381 4670
Fragments arranged by size:
4670 915 381
PfoI T'CCnGG_A
Cuts at: 238 1574 238
Size: 1336 4630
PmeI GTTT'AAAC
Cuts at: 2965 2965
Size: 5966
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4412 4444 4412
Size: 32 5934
PpuMI rG'GwC_Cy
Cuts at: 2313 2313
Size: 5966
PsiI TTA'TAA
Cuts at: 809 809
Size: 5966
PspOMI G'GGCC_C
Cuts at: 114 166 114
Size: 52 5914
PstI C_TGCA'G
Cuts at: 208 3013 3698 208
Size: 2805 685 2476
Fragments arranged by size:
2805 2476 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5966
PvuII CAG'CTG
Cuts at: 209 2747 3391 3751 209
Size: 2538 644 360 2424
Fragments arranged by size:
2538 2424 644 360
RsrII CG'GwC_CG
Cuts at: 4161 4161
Size: 5966
SacI G_AGCT'C
Cuts at: 2980 2980
Size: 5966
SalI G'TCGA_C
Cuts at: 3003 3003
Size: 5966
SapI GCTCTTCn'nnn_
Cuts at: 353 2065 3989 4199 353
Size: 1712 1924 210 2120
Fragments arranged by size:
2120 1924 1712 210
SbfI CC_TGCA'GG
Cuts at: 3013 3013
Size: 5966
ScaI AGT'ACT
Cuts at: 5483 5483
Size: 5966
SexAI A'CCwGG_T
Cuts at: 2870 2870
Size: 5966
SfcI C'TryA_G
Cuts at: 33 204 643 2126 2198 3009 3694 4662 4853
Size: 171 439 1483 72 811 685 968 191
Cuts at: 4853 33
Size: 1146
Fragments arranged by size:
1483 1146 968 811 685 439 191 171
72
SfoI GGC'GCC
Cuts at: 182 200 3398 3646 182
Size: 18 3198 248 2502
Fragments arranged by size:
3198 2502 248 18
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5966
SmaI CCC'GGG
Cuts at: 2988 2988
Size: 5966
SmlI C'TyrA_G
Cuts at: 675 927 3121 4036 4750 5012 675
Size: 252 2194 915 714 262 1629
Fragments arranged by size:
2194 1629 915 714 262 252
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5966
SphI G_CATG'C
Cuts at: 3019 3519 3019
Size: 500 5466
SspI AAT'ATT
Cuts at: 1708 2167 1708
Size: 459 5507
StuI AGG'CCT
Cuts at: 406 1268 1403 2881 406
Size: 862 135 1478 3491
Fragments arranged by size:
3491 1478 862 135
StyI C'CwwG_G
Cuts at: 2118 2604 3100 3454 2118
Size: 486 496 354 4630
Fragments arranged by size:
4630 496 486 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 298 413 3549 3682 4141 4350 298
Size: 115 3136 133 459 209 1914
Fragments arranged by size:
3136 1914 459 209 133 115
TatI w'GTAC_w
Cuts at: 1672 1807 2648 5481 1672
Size: 135 841 2833 2157
Fragments arranged by size:
2833 2157 841 135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5483 5515 5483
Size: 32 5934
Tth111I GACn'n_nGTC
Cuts at: 3763 3763
Size: 5966
XbaI T'CTAG_A
Cuts at: 354 1287 2997 354
Size: 933 1710 3323
Fragments arranged by size:
3323 1710 933
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3461 3461
Size: 5966
XmaI C'CCGG_G
Cuts at: 2986 2986
Size: 5966
XmnI GAAnn'nnTTC
Cuts at: 479 2696 479
Size: 2217 3749
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI Bsu36I BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NheI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AgeI AhdI AleI AlfI AloI AscI
BaeI BsaXI BsiWI BsmI BsmBI BspEI BssHII BstEII
BstXI BstZ17I BtgI ClaI FseI FspAI NcoI NotI
NruI PacI PmlI PshAI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI