Restriction map for
pF1KB5091
(Circular) (Six-base) MAPSORT of: pF1KB5091.seq Check: 7707 from: 1 to: 4925
pF1KB5091 4925 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 1963 1963
Size: 4925
Acc65I G'GTAC_C
Cuts at: 1941 1941
Size: 4925
AclI AA'CG_TT
Cuts at: 365 1905 4765 365
Size: 1540 2860 525
Fragments arranged by size:
2860 1540 525
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1501 2750 3182 3535 4197 1501
Size: 1249 432 353 662 2229
Fragments arranged by size:
2229 1249 662 432 353
AflIII A'CryG_T
Cuts at: 2277 4077 4358 2277
Size: 1800 281 2844
Fragments arranged by size:
2844 1800 281
AleI CACnn'nnGTG
Cuts at: 1121 1121
Size: 4925
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 183 183
Size: 4925
AlwNI CAG_nnn'CTG
Cuts at: 2230 3668 2230
Size: 1438 3487
ApaLI G'TGCA_C
Cuts at: 328 3763 328
Size: 3435 1490
ApoI r'AATT_y
Cuts at: 280 1144 1204 1253 1929 4278 4534 280
Size: 864 60 49 676 2349 256 671
Fragments arranged by size:
2349 864 676 671 256 60 49
AseI AT'TA_AT
Cuts at: 20 20
Size: 4925
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4925
AvaI C'yCGr_G
Cuts at: 1945 1945
Size: 4925
BamHI G'GATC_C
Cuts at: 1950 1950
Size: 4925
BanI G'GyrC_C
Cuts at: 948 1941 2355 2603 2638 948
Size: 993 414 248 35 3235
Fragments arranged by size:
3235 993 414 248 35
BanII G_rGCy'C
Cuts at: 519 646 1826 1939 2969 519
Size: 127 1180 113 1030 2475
Fragments arranged by size:
2475 1180 1030 127 113
BbeI G_GCGC'C
Cuts at: 2359 2607 2359
Size: 248 4677
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4891
BciVI GTATCCnnnnn_n'
Cuts at: 860 1586 2823 3879 4925 860
Size: 726 1237 1056 1046 860
Fragments arranged by size:
1237 1056 1046 860 726
BclI T'GATC_A
Cuts at: 2445 2445
Size: 4925
BglII A'GATC_T
Cuts at: 2440 4477 2440
Size: 2037 2888
BlpI GC'TnA_GC
Cuts at: 148 2037 148
Size: 1889 3036
Bme1580I G_kGCm'C
Cuts at: 332 2550 2643 3767 332
Size: 2218 93 1124 1490
Fragments arranged by size:
2218 1490 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1320 2137 2218 2553 4697 1320
Size: 817 81 335 2144 1548
Fragments arranged by size:
2144 1548 817 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3292 3292
Size: 4925
Bpu10I CC'TnA_GC
Cuts at: 905 905
Size: 4925
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 881 1140 2101 2980 3694 3992 881
Size: 259 961 879 714 298 1814
Fragments arranged by size:
1814 961 879 714 298 259
BsaI GGTCTCn'nnnn_
Cuts at: 4629 4629
Size: 4925
BsaAI yAC'GTr
Cuts at: 2278 2278
Size: 4925
BsaBI GATnn'nnATC
Cuts at: 6 1880 2439 4476 6
Size: 1874 559 2037 455
Fragments arranged by size:
2037 1874 559 455
BsaHI Gr'CG_yC
Cuts at: 2356 2604 3306 4802 2356
Size: 248 702 1496 2479
Fragments arranged by size:
2479 1496 702 248
BsaWI w'CCGG_w
Cuts at: 276 1864 2114 2635 3313 3724 3871 4145 276
Size: 1588 250 521 678 411 147 274 1056
Fragments arranged by size:
1588 1056 678 521 411 274 250 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 884 884
Size: 4925
BseYI C'CCAG_C
Cuts at: 479 671 735 2350 3108 3773 4181 479
Size: 192 64 1615 758 665 408 1223
Fragments arranged by size:
1615 1223 758 665 408 192 64
BsiEI CG_ry'CG
Cuts at: 104 2467 2513 3743 104
Size: 2363 46 1230 1286
Fragments arranged by size:
2363 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 332 1647 1826 1939 2717 2907 3767 332
Size: 1315 179 113 778 190 860 1490
Fragments arranged by size:
1490 1315 860 778 190 179 113
BsmI GAATG_Cn'
Cuts at: 661 661
Size: 4925
Bsp1286I G_dGCh'C
Cuts at: 332 519 646 1647 1826 1939 2550 2643 2717
Size: 187 127 1001 179 113 611 93 74
Cuts at: 2717 2907 2969 3767 332
Size: 190 62 798 1490
Fragments arranged by size:
1490 1001 798 611 190 187 179 127
113 93 74 62
BspEI T'CCGG_A
Cuts at: 1864 1864
Size: 4925
BspHI T'CATG_A
Cuts at: 1257 3357 1257
Size: 2100 2825
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1975 2189 2491 2872 1975
Size: 214 302 381 4028
Fragments arranged by size:
4028 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1862 3217 3271 4755 4920 50
Size: 1812 1355 54 1484 165 55
Fragments arranged by size:
1812 1484 1355 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2837 2837
Size: 4925
BsrFI r'CCGG_y
Cuts at: 2923 3104 2923
Size: 181 4744
BssSI C'ACGA_G
Cuts at: 3196 3904 3196
Size: 708 4217
BstAPI GCAn_nnn'nTGC
Cuts at: 667 2302 667
Size: 1635 3290
BstBI TT'CG_AA
Cuts at: 390 3286 390
Size: 2896 2029
BstXI CCAn_nnnn'nTGG
Cuts at: 735 735
Size: 4925
BstYI r'GATC_y
Cuts at: 1 1815 1950 2417 2440 2775 3021 3327 3339
Size: 1814 135 467 23 335 246 306 12
Cuts at: 3339 3425 4477 1
Size: 86 1052 449
Fragments arranged by size:
1814 1052 467 449 335 306 246 135
86 23 12
BtgI C'CryG_G
Cuts at: 741 1628 741
Size: 887 4038
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1019 2875 3056 3219 1019
Size: 1856 181 163 2725
Fragments arranged by size:
2725 1856 181 163
BtsI GCAGTG_nn'
Cuts at: 1745 2168 1745
Size: 423 4502
ClaI AT'CG_AT
Cuts at: 1559 1559
Size: 4925
DraI TTT'AAA
Cuts at: 1924 1924
Size: 4925
DrdI GACnn_nn'nnGTC
Cuts at: 2631 3975 2631
Size: 1344 3581
EaeI y'GGCC_r
Cuts at: 1603 2510 2684 3075 3102 4447 1603
Size: 907 174 391 27 1345 2081
Fragments arranged by size:
2081 1345 907 391 174 27
EagI C'GGCC_G
Cuts at: 2510 2510
Size: 4925
EarI CTCTTCn'nnn_
Cuts at: 2948 3158 2948
Size: 210 4715
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3133 3875 4021 4735 3133
Size: 742 146 714 3323
Fragments arranged by size:
3323 742 714 146
EcoICRI GAG'CTC
Cuts at: 1824 1937 1824
Size: 113 4812
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1501 2750 3182 3535 4197 1501
Size: 1249 432 353 662 2229
Fragments arranged by size:
2229 1249 662 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 805 805
Size: 4925
EcoO109I rG'GnC_Cy
Cuts at: 2064 2064
Size: 4925
EcoRI G'AATT_C
Cuts at: 1929 1929
Size: 4925
EcoRV GAT'ATC
Cuts at: 1878 1878
Size: 4925
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2944 2944
Size: 4925
FspI TGC'GCA
Cuts at: 2706 2706
Size: 4925
HaeII r_GCGC'y
Cuts at: 148 2359 2607 3837 4608 148
Size: 2211 248 1230 771 465
Fragments arranged by size:
2211 1230 771 465 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 426 458 837 869 876 908 1113 1145 2767
Size: 32 379 32 7 32 205 32 1622
Cuts at: 2767 2797 2799 2829 426
Size: 30 2 30 2522
Fragments arranged by size:
2522 1622 379 205 32 32 32 32
30 30 7 2
HincII GTy'rAC
Cuts at: 416 1964 416
Size: 1548 3377
KasI G'GCGC_C
Cuts at: 2355 2603 2355
Size: 248 4677
KpnI G_GTAC'C
Cuts at: 1945 1945
Size: 4925
MluI A'CGCG_T
Cuts at: 4358 4358
Size: 4925
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1517 1620 3684 3868 1517
Size: 103 2064 184 2574
Fragments arranged by size:
2574 2064 184 103
MscI TGG'CCA
Cuts at: 2686 2686
Size: 4925
MslI CAynn'nnrTG
Cuts at: 1121 2914 3041 1121
Size: 1793 127 3005
Fragments arranged by size:
3005 1793 127
MspA1I CmG'CkG
Cuts at: 2036 2350 2710 3494 3739 2036
Size: 314 360 784 245 3222
Fragments arranged by size:
3222 784 360 314 245
NaeI GCC'GGC
Cuts at: 3106 3106
Size: 4925
NarI GG'CG_CC
Cuts at: 2356 2604 2356
Size: 248 4677
NcoI C'CATG_G
Cuts at: 741 1628 741
Size: 887 4038
NgoMIV G'CCGG_C
Cuts at: 3104 3104
Size: 4925
NspI r_CATG'y
Cuts at: 1978 2478 4081 1978
Size: 500 1603 2822
Fragments arranged by size:
2822 1603 500
PasI CC'CwG_GG
Cuts at: 639 639
Size: 4925
PciI A'CATG_T
Cuts at: 4077 4077
Size: 4925
PflMI CCAn_nnn'nTGG
Cuts at: 747 977 747
Size: 230 4695
PmeI GTTT'AAAC
Cuts at: 1924 1924
Size: 4925
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1033 1065 3371 3403 1033
Size: 32 2306 32 2555
Fragments arranged by size:
2555 2306 32 32
PstI C_TGCA'G
Cuts at: 1533 1972 2657 1533
Size: 439 685 3801
Fragments arranged by size:
3801 685 439
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4925
PvuII CAG'CTG
Cuts at: 2350 2710 2350
Size: 360 4565
RsrII CG'GwC_CG
Cuts at: 3120 3120
Size: 4925
SacI G_AGCT'C
Cuts at: 1826 1939 1826
Size: 113 4812
SalI G'TCGA_C
Cuts at: 1962 1962
Size: 4925
SapI GCTCTTCn'nnn_
Cuts at: 2948 3158 2948
Size: 210 4715
SbfI CC_TGCA'GG
Cuts at: 1972 1972
Size: 4925
ScaI AGT'ACT
Cuts at: 1892 4442 1892
Size: 2550 2375
SfcI C'TryA_G
Cuts at: 33 1529 1968 2653 3621 3812 33
Size: 1496 439 685 968 191 1146
Fragments arranged by size:
1496 1146 968 685 439 191
SfoI GGC'GCC
Cuts at: 2357 2605 2357
Size: 248 4677
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4925
SmaI CCC'GGG
Cuts at: 1947 1947
Size: 4925
SmlI C'TyrA_G
Cuts at: 896 1119 2080 2995 3709 3971 896
Size: 223 961 915 714 262 1850
Fragments arranged by size:
1850 961 915 714 262 223
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4925
SphI G_CATG'C
Cuts at: 1978 2478 1978
Size: 500 4425
StyI C'CwwG_G
Cuts at: 551 741 1628 2059 2413 551
Size: 190 887 431 354 3063
Fragments arranged by size:
3063 887 431 354 190
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 194 544 2508 2641 3100 3309 194
Size: 350 1964 133 459 209 1810
Fragments arranged by size:
1964 1810 459 350 209 133
TatI w'GTAC_w
Cuts at: 1395 1890 4440 1395
Size: 495 2550 1880
Fragments arranged by size:
2550 1880 495
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4442 4474 4442
Size: 32 4893
Tth111I GACn'n_nGTC
Cuts at: 2722 2722
Size: 4925
XbaI T'CTAG_A
Cuts at: 1956 1956
Size: 4925
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2420 2420
Size: 4925
XmaI C'CCGG_G
Cuts at: 1945 1945
Size: 4925
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI BpmI BsaXI BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I CspCI DraIII FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI