Restriction map for
pF1KB5086
(Circular) (Six-base) MAPSORT of: pF1KB5086.seq Check: 8290 from: 1 to: 5093
pF1KB5086 5093 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 1858 2131 1858
Size: 273 4820
Acc65I G'GTAC_C
Cuts at: 2109 2109
Size: 5093
AclI AA'CG_TT
Cuts at: 1181 4933 1181
Size: 3752 1341
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1066 1087 1125 1228 1495 2918 3350 3703 4365
Size: 21 38 103 267 1423 432 353 662
Cuts at: 4365 1066
Size: 1794
Fragments arranged by size:
1794 1423 662 432 353 267 103 38
21
AfeI AGC'GCT
Cuts at: 1489 1489
Size: 5093
AflIII A'CryG_T
Cuts at: 1013 2445 4245 4526 1013
Size: 1432 1800 281 1580
Fragments arranged by size:
1800 1580 1432 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1444 1988 1444
Size: 544 4549
AlwNI CAG_nnn'CTG
Cuts at: 1062 1749 2084 2398 3836 1062
Size: 687 335 314 1438 2319
Fragments arranged by size:
2319 1438 687 335 314
ApaI G_GGCC'C
Cuts at: 1372 1873 1900 1372
Size: 501 27 4565
Fragments arranged by size:
4565 501 27
ApaLI G'TGCA_C
Cuts at: 1618 3931 1618
Size: 2313 2780
ApoI r'AATT_y
Cuts at: 2097 4446 4702 2097
Size: 2349 256 2488
Fragments arranged by size:
2488 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5093
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5093
AvaI C'yCGr_G
Cuts at: 142 156 1296 2113 142
Size: 14 1140 817 3122
Fragments arranged by size:
3122 1140 817 14
BamHI G'GATC_C
Cuts at: 2118 2118
Size: 5093
BanI G'GyrC_C
Cuts at: 1783 2109 2523 2771 2806 1783
Size: 326 414 248 35 4070
Fragments arranged by size:
4070 414 326 248 35
BanII G_rGCy'C
Cuts at: 149 289 1372 1873 1900 2107 3137 149
Size: 140 1083 501 27 207 1030 2105
Fragments arranged by size:
2105 1083 1030 501 207 140 27
BbeI G_GCGC'C
Cuts at: 2527 2775 2527
Size: 248 4845
BbsI GAAGACnn'nnnn_
Cuts at: 864 864
Size: 5093
BbvCI CC'TCA_GC
Cuts at: 1994 1994
Size: 5093
BciVI GTATCCnnnnn_n'
Cuts at: 2991 4047 5093 2991
Size: 1056 1046 2991
Fragments arranged by size:
2991 1056 1046
BclI T'GATC_A
Cuts at: 1340 2613 1340
Size: 1273 3820
BglI GCCn_nnn'nGGC
Cuts at: 178 776 178
Size: 598 4495
BglII A'GATC_T
Cuts at: 797 1172 1200 2608 4645 797
Size: 375 28 1408 2037 1245
Fragments arranged by size:
2037 1408 1245 375 28
BlpI GC'TnA_GC
Cuts at: 2205 2205
Size: 5093
Bme1580I G_kGCm'C
Cuts at: 1162 1372 1622 1873 1900 2718 2811 3935 1162
Size: 210 250 251 27 818 93 1124 2320
Fragments arranged by size:
2320 1124 818 251 250 210 93 27
BmrI ACTGGGnnnn_n'
Cuts at: 1691 2305 2386 2721 4865 1691
Size: 614 81 335 2144 1919
Fragments arranged by size:
2144 1919 614 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 168 3460 168
Size: 3292 1801
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 577 805 1786 1955 1972 577
Size: 228 981 169 17 3698
Fragments arranged by size:
3698 981 228 169 17
Bpu10I CC'TnA_GC
Cuts at: 1994 2000 1994
Size: 6 5087
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2269 3148 3862 4160 2269
Size: 879 714 298 3202
Fragments arranged by size:
3202 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 349 4797 349
Size: 4448 645
BsaAI yAC'GTr
Cuts at: 2446 2446
Size: 5093
BsaBI GATnn'nnATC
Cuts at: 6 2607 4644 6
Size: 2601 2037 455
Fragments arranged by size:
2601 2037 455
BsaHI Gr'CG_yC
Cuts at: 2524 2772 3474 4970 2524
Size: 248 702 1496 2647
Fragments arranged by size:
2647 1496 702 248
BsaWI w'CCGG_w
Cuts at: 527 1499 2282 2803 3481 3892 4039 4313 527
Size: 972 783 521 678 411 147 274 1307
Fragments arranged by size:
1307 972 783 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1213 1243 1273 1303 1213
Size: 30 30 30 5003
Fragments arranged by size:
5003 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1216 1267 1270 1789 1938 1216
Size: 51 3 519 149 4371
Fragments arranged by size:
4371 519 149 51 3
BseYI C'CCAG_C
Cuts at: 148 499 1365 2005 2518 3276 3941 4349 148
Size: 351 866 640 513 758 665 408 892
Fragments arranged by size:
892 866 758 665 640 513 408 351
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1123 1717 1123
Size: 594 4499
BsiEI CG_ry'CG
Cuts at: 104 182 2635 2681 3911 104
Size: 78 2453 46 1230 1286
Fragments arranged by size:
2453 1286 1230 78 46
BsiHKAI G_wGCw'C
Cuts at: 1622 1926 2107 2885 3075 3935 1622
Size: 304 181 778 190 860 2780
Fragments arranged by size:
2780 860 778 304 190 181
BsmBI CGTCTCn'nnnn_
Cuts at: 1880 1880
Size: 5093
Bsp1286I G_dGCh'C
Cuts at: 149 289 1162 1372 1622 1873 1900 1926 2107
Size: 140 873 210 250 251 27 26 181
Cuts at: 2107 2718 2811 2885 3075 3137 3935 149
Size: 611 93 74 190 62 798 1307
Fragments arranged by size:
1307 873 798 611 251 250 210 190
181 140 93 74 62 27 26
BspEI T'CCGG_A
Cuts at: 527 1499 527
Size: 972 4121
BspHI T'CATG_A
Cuts at: 846 3525 846
Size: 2679 2414
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1739 2143 2357 2659 3040 1739
Size: 404 214 302 381 3792
Fragments arranged by size:
3792 404 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3385 3439 4923 5088 50
Size: 3335 54 1484 165 55
Fragments arranged by size:
3335 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 753 930 1397 3005 753
Size: 177 467 1608 2841
Fragments arranged by size:
2841 1608 467 177
BsrFI r'CCGG_y
Cuts at: 117 2018 3091 3272 117
Size: 1901 1073 181 1938
Fragments arranged by size:
1938 1901 1073 181
BsrGI T'GTAC_A
Cuts at: 1698 1698
Size: 5093
BssSI C'ACGA_G
Cuts at: 3364 4072 3364
Size: 708 4385
BstAPI GCAn_nnn'nTGC
Cuts at: 1468 2470 1468
Size: 1002 4091
BstBI TT'CG_AA
Cuts at: 3454 3454
Size: 5093
BstXI CCAn_nnnn'nTGG
Cuts at: 273 711 273
Size: 438 4655
BstYI r'GATC_y
Cuts at: 1 797 1088 1172 1200 2118 2585 2608 2943
Size: 796 291 84 28 918 467 23 335
Cuts at: 2943 3189 3495 3507 3593 4645 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 918 796 467 449 335 306 291
246 86 84 28 23 12
BtgI C'CryG_G
Cuts at: 107 173 195 771 1613 107
Size: 66 22 576 842 3587
Fragments arranged by size:
3587 842 576 66 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1402 3043 3224 3387 1402
Size: 1641 181 163 3108
Fragments arranged by size:
3108 1641 181 163
BtsI GCAGTG_nn'
Cuts at: 578 714 2016 2336 578
Size: 136 1302 320 3335
Fragments arranged by size:
3335 1302 320 136
DraI TTT'AAA
Cuts at: 2092 2092
Size: 5093
DraIII CAC_nnn'GTG
Cuts at: 2046 2046
Size: 5093
DrdI GACnn_nn'nnGTC
Cuts at: 118 2799 4143 118
Size: 2681 1344 1068
Fragments arranged by size:
2681 1344 1068
EaeI y'GGCC_r
Cuts at: 164 179 642 1452 2678 2852 3243 3270 4615
Size: 15 463 810 1226 174 391 27 1345
Cuts at: 4615 164
Size: 642
Fragments arranged by size:
1345 1226 810 642 463 391 174 27
15
EagI C'GGCC_G
Cuts at: 179 2678 179
Size: 2499 2594
EarI CTCTTCn'nnn_
Cuts at: 436 673 3116 3326 436
Size: 237 2443 210 2203
Fragments arranged by size:
2443 2203 237 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 466 661 3301 4043 4189 4903 466
Size: 195 2640 742 146 714 656
Fragments arranged by size:
2640 742 714 656 195 146
EcoICRI GAG'CTC
Cuts at: 2105 2105
Size: 5093
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 577 805 1066 1087 1125 1228 1495 1786 1955
Size: 228 261 21 38 103 267 291 169
Cuts at: 1955 1972 2918 3350 3703 4365 577
Size: 17 946 432 353 662 1305
Fragments arranged by size:
1305 946 662 432 353 291 267 261
228 169 103 38 21 17
EcoO109I rG'GnC_Cy
Cuts at: 615 1869 1870 1896 2060 2232 615
Size: 1254 1 26 164 172 3476
Fragments arranged by size:
3476 1254 172 164 26 1
EcoRI G'AATT_C
Cuts at: 2097 2097
Size: 5093
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3112 3112
Size: 5093
FspI TGC'GCA
Cuts at: 1678 2874 1678
Size: 1196 3897
HaeII r_GCGC'y
Cuts at: 238 1062 1491 2040 2527 2775 4005 4776 238
Size: 824 429 549 487 248 1230 771 555
Fragments arranged by size:
1230 824 771 555 549 487 429 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 873 905 1273 1305 1325 1357 1930 1962 2935
Size: 32 368 32 20 32 573 32 973
Cuts at: 2935 2965 2967 2997 873
Size: 30 2 30 2969
Fragments arranged by size:
2969 973 573 368 32 32 32 32
30 30 20 2
HincII GTy'rAC
Cuts at: 1859 2132 1859
Size: 273 4820
KasI G'GCGC_C
Cuts at: 2523 2771 2523
Size: 248 4845
KpnI G_GTAC'C
Cuts at: 2113 2113
Size: 5093
MluI A'CGCG_T
Cuts at: 4526 4526
Size: 5093
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1160 1202 3852 4036 1160
Size: 42 2650 184 2217
Fragments arranged by size:
2650 2217 184 42
MscI TGG'CCA
Cuts at: 1454 2854 1454
Size: 1400 3693
MslI CAynn'nnrTG
Cuts at: 680 910 1513 3082 3209 680
Size: 230 603 1569 127 2564
Fragments arranged by size:
2564 1569 603 230 127
MspA1I CmG'CkG
Cuts at: 175 1746 1972 2081 2204 2518 2878 3662 3907
Size: 1571 226 109 123 314 360 784 245
Cuts at: 3907 175
Size: 1361
Fragments arranged by size:
1571 1361 784 360 314 245 226 123
109
NaeI GCC'GGC
Cuts at: 2020 3274 2020
Size: 1254 3839
NarI GG'CG_CC
Cuts at: 2524 2772 2524
Size: 248 4845
NcoI C'CATG_G
Cuts at: 107 1613 107
Size: 1506 3587
NgoMIV G'CCGG_C
Cuts at: 2018 3272 2018
Size: 1254 3839
NotI GC'GGCC_GC
Cuts at: 179 179
Size: 5093
NspI r_CATG'y
Cuts at: 909 1017 1431 2146 2646 4249 909
Size: 108 414 715 500 1603 1753
Fragments arranged by size:
1753 1603 715 500 414 108
PasI CC'CwG_GG
Cuts at: 2055 2055
Size: 5093
PciI A'CATG_T
Cuts at: 1013 4245 1013
Size: 3232 1861
PflMI CCAn_nnn'nTGG
Cuts at: 1614 1749 1614
Size: 135 4958
PfoI T'CCnGG_A
Cuts at: 554 1757 554
Size: 1203 3890
PmeI GTTT'AAAC
Cuts at: 2092 2092
Size: 5093
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3539 3571 3539
Size: 32 5061
PpuMI rG'GwC_Cy
Cuts at: 615 615
Size: 5093
PshAI GACnn'nnGTC
Cuts at: 1016 1016
Size: 5093
PspOMI G'GGCC_C
Cuts at: 1368 1869 1896 1368
Size: 501 27 4565
Fragments arranged by size:
4565 501 27
PstI C_TGCA'G
Cuts at: 220 2140 2825 220
Size: 1920 685 2488
Fragments arranged by size:
2488 1920 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5093
PvuII CAG'CTG
Cuts at: 1972 2518 2878 1972
Size: 546 360 4187
Fragments arranged by size:
4187 546 360
RsrII CG'GwC_CG
Cuts at: 3288 3288
Size: 5093
SacI G_AGCT'C
Cuts at: 2107 2107
Size: 5093
SacII CC_GC'GG
Cuts at: 176 176
Size: 5093
SalI G'TCGA_C
Cuts at: 1857 2130 1857
Size: 273 4820
SapI GCTCTTCn'nnn_
Cuts at: 436 3116 3326 436
Size: 2680 210 2203
Fragments arranged by size:
2680 2203 210
SbfI CC_TGCA'GG
Cuts at: 2140 2140
Size: 5093
ScaI AGT'ACT
Cuts at: 4610 4610
Size: 5093
SexAI A'CCwGG_T
Cuts at: 521 521
Size: 5093
SfcI C'TryA_G
Cuts at: 33 216 1245 1952 2136 2821 3789 3980 33
Size: 183 1029 707 184 685 968 191 1146
Fragments arranged by size:
1146 1029 968 707 685 191 184 183
SfiI GGCCn_nnn'nGGCC
Cuts at: 178 178
Size: 5093
SfoI GGC'GCC
Cuts at: 2525 2773 2525
Size: 248 4845
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5093
SmaI CCC'GGG
Cuts at: 158 1298 2115 158
Size: 1140 817 3136
Fragments arranged by size:
3136 1140 817
SmlI C'TyrA_G
Cuts at: 2248 3163 3877 4139 2248
Size: 915 714 262 3202
Fragments arranged by size:
3202 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5093
SphI G_CATG'C
Cuts at: 909 1431 2146 2646 909
Size: 522 715 500 3356
Fragments arranged by size:
3356 715 522 500
StuI AGG'CCT
Cuts at: 424 1337 424
Size: 913 4180
StyI C'CwwG_G
Cuts at: 107 1379 1613 1772 2227 2581 107
Size: 1272 234 159 455 354 2619
Fragments arranged by size:
2619 1272 455 354 234 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2676 2809 3268 3477 2676
Size: 133 459 209 4292
Fragments arranged by size:
4292 459 209 133
TatI w'GTAC_w
Cuts at: 1698 4608 1698
Size: 2910 2183
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4610 4642 4610
Size: 32 5061
Tth111I GACn'n_nGTC
Cuts at: 2890 2890
Size: 5093
XbaI T'CTAG_A
Cuts at: 2124 2124
Size: 5093
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 954 1656 2588 954
Size: 702 932 3459
Fragments arranged by size:
3459 932 702
XmaI C'CCGG_G
Cuts at: 156 1296 2113 156
Size: 1140 817 3136
Fragments arranged by size:
3136 1140 817
XmnI GAAnn'nnTTC
Cuts at: 667 1213 667
Size: 546 4547
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AloI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NruI
NsiI PacI PmlI PsiI PspXI PsrI SanDI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI