Restriction map for
pF1KB5083
(Circular) (Six-base) MAPSORT of: pF1KB5083.seq Check: 1591 from: 1 to: 5168
pF1KB5083 5168 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 329 428 919 997 2206 329
Size: 99 491 78 1209 3291
Fragments arranged by size:
3291 1209 491 99 78
Acc65I G'GTAC_C
Cuts at: 832 1658 2184 832
Size: 826 526 3816
Fragments arranged by size:
3816 826 526
AclI AA'CG_TT
Cuts at: 2162 5008 2162
Size: 2846 2322
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 875 1900 2993 3425 4172 4440 875
Size: 1025 1093 432 747 268 1603
Fragments arranged by size:
1603 1093 1025 747 432 268
AfeI AGC'GCT
Cuts at: 349 349
Size: 5168
AflIII A'CryG_T
Cuts at: 2520 3624 4601 2520
Size: 1104 977 3087
Fragments arranged by size:
3087 1104 977
AgeI A'CCGG_T
Cuts at: 3556 3556
Size: 5168
AhdI GACnn_n'nnGTC
Cuts at: 1134 1134
Size: 5168
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1055 1055
Size: 5168
AlwNI CAG_nnn'CTG
Cuts at: 171 1213 2473 4040 171
Size: 1042 1260 1567 1299
Fragments arranged by size:
1567 1299 1260 1042
ApaI G_GGCC'C
Cuts at: 903 903
Size: 5168
ApaLI G'TGCA_C
Cuts at: 3938 3938
Size: 5168
ApoI r'AATT_y
Cuts at: 512 1191 1977 2172 4521 4777 512
Size: 679 786 195 2349 256 903
Fragments arranged by size:
2349 903 786 679 256 195
AseI AT'TA_AT
Cuts at: 20 746 20
Size: 726 4442
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5168
AvaI C'yCGr_G
Cuts at: 223 300 2188 223
Size: 77 1888 3203
Fragments arranged by size:
3203 1888 77
BamHI G'GATC_C
Cuts at: 2193 2193
Size: 5168
BanI G'GyrC_C
Cuts at: 832 1658 1963 2184 2598 2846 2881 832
Size: 826 305 221 414 248 35 3119
Fragments arranged by size:
3119 826 414 305 248 221 35
BanII G_rGCy'C
Cuts at: 903 1367 2182 3212 903
Size: 464 815 1030 2859
Fragments arranged by size:
2859 1030 815 464
BbeI G_GCGC'C
Cuts at: 2602 2850 2602
Size: 248 4920
BbsI GAAGACnn'nnnn_
Cuts at: 1130 1292 1505 1902 1130
Size: 162 213 397 4396
Fragments arranged by size:
4396 397 213 162
BciVI GTATCCnnnnn_n'
Cuts at: 3066 3827 5168 3066
Size: 761 1341 3066
Fragments arranged by size:
3066 1341 761
BclI T'GATC_A
Cuts at: 1989 2688 1989
Size: 699 4469
BglII A'GATC_T
Cuts at: 311 720 1869 2683 4720 311
Size: 409 1149 814 2037 759
Fragments arranged by size:
2037 1149 814 759 409
BlpI GC'TnA_GC
Cuts at: 1597 2280 1597
Size: 683 4485
Bme1580I G_kGCm'C
Cuts at: 263 903 2793 2886 3942 263
Size: 640 1890 93 1056 1489
Fragments arranged by size:
1890 1489 1056 640 93
BmrI ACTGGGnnnn_n'
Cuts at: 350 1070 2380 2461 2796 4940 350
Size: 720 1310 81 335 2144 578
Fragments arranged by size:
2144 1310 720 578 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3535 3535
Size: 5168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1351 1351
Size: 5168
Bpu10I CC'TnA_GC
Cuts at: 1300 1793 1300
Size: 493 4675
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2344 3223 3715 4013 2344
Size: 879 492 298 3499
Fragments arranged by size:
3499 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4872 4872
Size: 5168
BsaAI yAC'GTr
Cuts at: 2521 2521
Size: 5168
BsaBI GATnn'nnATC
Cuts at: 6 2682 4719 6
Size: 2676 2037 455
Fragments arranged by size:
2676 2037 455
BsaHI Gr'CG_yC
Cuts at: 2599 2847 3549 5045 2599
Size: 248 702 1496 2722
Fragments arranged by size:
2722 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1125 2357 2878 3556 3830 3977 4388 1125
Size: 1232 521 678 274 147 411 1905
Fragments arranged by size:
1905 1232 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1773 1803 1773
Size: 30 5138
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1375 1861 1375
Size: 486 4682
BseYI C'CCAG_C
Cuts at: 171 369 1182 1339 1776 2593 3351 3928 4424
Size: 198 813 157 437 817 758 577 496
Cuts at: 4424 171
Size: 915
Fragments arranged by size:
915 817 813 758 577 496 437 198
157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 556
Size: 5168
BsiEI CG_ry'CG
Cuts at: 104 211 2710 2756 3964 104
Size: 107 2499 46 1208 1308
Fragments arranged by size:
2499 1308 1208 107 46
BsiHKAI G_wGCw'C
Cuts at: 1367 1603 1707 2182 2960 3150 3942 1367
Size: 236 104 475 778 190 792 2593
Fragments arranged by size:
2593 792 778 475 236 190 104
BsmI GAATG_Cn'
Cuts at: 1495 1580 1495
Size: 85 5083
Bsp1286I G_dGCh'C
Cuts at: 263 903 1367 1603 1707 2182 2793 2886 2960
Size: 640 464 236 104 475 611 93 74
Cuts at: 2960 3150 3212 3942 263
Size: 190 62 730 1489
Fragments arranged by size:
1489 730 640 611 475 464 236 190
104 93 74 62
BspEI T'CCGG_A
Cuts at: 1125 4388 1125
Size: 3263 1905
BspHI T'CATG_A
Cuts at: 696 4344 696
Size: 3648 1520
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2218 2432 2734 3115 2218
Size: 214 302 381 4271
Fragments arranged by size:
4271 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3460 3514 4998 5163 50
Size: 3410 54 1484 165 55
Fragments arranged by size:
3410 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1012 3080 1012
Size: 2068 3100
BsrFI r'CCGG_y
Cuts at: 3166 3347 3556 3166
Size: 181 209 4778
Fragments arranged by size:
4778 209 181
BssSI C'ACGA_G
Cuts at: 3439 3797 3439
Size: 358 4810
BstAPI GCAn_nnn'nTGC
Cuts at: 182 534 1213 1490 1786 2545 182
Size: 352 679 277 296 759 2805
Fragments arranged by size:
2805 759 679 352 296 277
BstBI TT'CG_AA
Cuts at: 1893 3529 1893
Size: 1636 3532
BstEII G'GTnAC_C
Cuts at: 1617 1617
Size: 5168
BstYI r'GATC_y
Cuts at: 1 311 720 1869 2193 2660 2683 3018 3264
Size: 310 409 1149 324 467 23 335 246
Cuts at: 3264 4276 4362 4374 4720 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1149 1012 467 449 409 346 335 324
310 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 920 998 920
Size: 78 5090
Bsu36I CC'TnA_GG
Cuts at: 664 664
Size: 5168
BtgI C'CryG_G
Cuts at: 107 755 1508 107
Size: 648 753 3767
Fragments arranged by size:
3767 753 648
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3118 3299 3462 3118
Size: 181 163 4824
Fragments arranged by size:
4824 181 163
BtsI GCAGTG_nn'
Cuts at: 1491 2411 1491
Size: 920 4248
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1368 1403 1368
Size: 35 5133
DraI TTT'AAA
Cuts at: 2167 2167
Size: 5168
DrdI GACnn_nn'nnGTC
Cuts at: 1678 2874 3732 1678
Size: 1196 858 3114
Fragments arranged by size:
3114 1196 858
EaeI y'GGCC_r
Cuts at: 208 1758 2753 2927 3318 3345 4690 208
Size: 1550 995 174 391 27 1345 686
Fragments arranged by size:
1550 1345 995 686 391 174 27
EagI C'GGCC_G
Cuts at: 208 2753 208
Size: 2545 2623
EarI CTCTTCn'nnn_
Cuts at: 3191 3401 3191
Size: 210 4958
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3376 3686 3832 4978 3376
Size: 310 146 1146 3566
Fragments arranged by size:
3566 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1365 2180 1365
Size: 815 4353
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 875 1351 1900 2993 3425 4172 4440 875
Size: 476 549 1093 432 747 268 1603
Fragments arranged by size:
1603 1093 747 549 476 432 268
EcoO109I rG'GnC_Cy
Cuts at: 900 938 1938 2307 900
Size: 38 1000 369 3761
Fragments arranged by size:
3761 1000 369 38
EcoRI G'AATT_C
Cuts at: 512 2172 512
Size: 1660 3508
EcoRV GAT'ATC
Cuts at: 2060 2060
Size: 5168
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 399 1212 2047 3187 399
Size: 813 835 1140 2380
Fragments arranged by size:
2380 1140 835 813
FspI TGC'GCA
Cuts at: 2949 2949
Size: 5168
HaeII r_GCGC'y
Cuts at: 172 351 2602 2850 3872 4851 172
Size: 179 2251 248 1022 979 489
Fragments arranged by size:
2251 1022 979 489 248 179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 647 679 1120 1152 1889 1921 2044 2076 3010
Size: 32 441 32 737 32 123 32 934
Cuts at: 3010 3040 3042 3072 647
Size: 30 2 30 2743
Fragments arranged by size:
2743 934 737 441 123 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 330 638 2207 330
Size: 308 1569 3291
Fragments arranged by size:
3291 1569 308
HindIII A'AGCT_T
Cuts at: 1297 1297
Size: 5168
KasI G'GCGC_C
Cuts at: 2598 2846 2598
Size: 248 4920
KpnI G_GTAC'C
Cuts at: 836 1662 2188 836
Size: 826 526 3816
Fragments arranged by size:
3816 826 526
MluI A'CGCG_T
Cuts at: 4601 4601
Size: 5168
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3839 4023 3839
Size: 184 4984
MscI TGG'CCA
Cuts at: 1760 2929 1760
Size: 1169 3999
MslI CAynn'nnrTG
Cuts at: 154 527 881 1611 3157 3284 154
Size: 373 354 730 1546 127 2038
Fragments arranged by size:
2038 1546 730 373 354 127
MspA1I CmG'CkG
Cuts at: 1210 1472 1596 2279 2593 2953 3966 4211 1210
Size: 262 124 683 314 360 1013 245 2167
Fragments arranged by size:
2167 1013 683 360 314 262 245 124
NaeI GCC'GGC
Cuts at: 3349 3349
Size: 5168
NarI GG'CG_CC
Cuts at: 2599 2847 2599
Size: 248 4920
NcoI C'CATG_G
Cuts at: 107 755 1508 107
Size: 648 753 3767
Fragments arranged by size:
3767 753 648
NgoMIV G'CCGG_C
Cuts at: 3347 3347
Size: 5168
NotI GC'GGCC_GC
Cuts at: 208 208
Size: 5168
NspI r_CATG'y
Cuts at: 532 1566 2145 2221 2721 3628 532
Size: 1034 579 76 500 907 2072
Fragments arranged by size:
2072 1034 907 579 500 76
PasI CC'CwG_GG
Cuts at: 903 903
Size: 5168
PciI A'CATG_T
Cuts at: 3624 3624
Size: 5168
PmeI GTTT'AAAC
Cuts at: 2167 2167
Size: 5168
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4307 4339 4307
Size: 32 5136
PshAI GACnn'nnGTC
Cuts at: 779 779
Size: 5168
PspOMI G'GGCC_C
Cuts at: 899 899
Size: 5168
PstI C_TGCA'G
Cuts at: 595 2215 2900 595
Size: 1620 685 2863
Fragments arranged by size:
2863 1620 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5168
PvuII CAG'CTG
Cuts at: 1210 1472 1596 2593 2953 1210
Size: 262 124 997 360 3425
Fragments arranged by size:
3425 997 360 262 124
RsrII CG'GwC_CG
Cuts at: 3363 3363
Size: 5168
SacI G_AGCT'C
Cuts at: 1367 2182 1367
Size: 815 4353
SalI G'TCGA_C
Cuts at: 328 2205 328
Size: 1877 3291
SapI GCTCTTCn'nnn_
Cuts at: 3191 3401 3191
Size: 210 4958
SbfI CC_TGCA'GG
Cuts at: 2215 2215
Size: 5168
ScaI AGT'ACT
Cuts at: 4685 4685
Size: 5168
SfcI C'TryA_G
Cuts at: 33 591 1473 2010 2211 2896 3889 4080 33
Size: 558 882 537 201 685 993 191 1121
Fragments arranged by size:
1121 993 882 685 558 537 201 191
SfoI GGC'GCC
Cuts at: 2600 2848 2600
Size: 248 4920
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5168
SmaI CCC'GGG
Cuts at: 2190 2190
Size: 5168
SmlI C'TyrA_G
Cuts at: 2323 3238 3730 3992 2323
Size: 915 492 262 3499
Fragments arranged by size:
3499 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5168
SphI G_CATG'C
Cuts at: 2221 2721 2221
Size: 500 4668
StyI C'CwwG_G
Cuts at: 107 755 803 1508 2302 2656 107
Size: 648 48 705 794 354 2619
Fragments arranged by size:
2619 794 705 648 354 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 769 2751 2884 3343 3552 769
Size: 1982 133 459 209 2385
Fragments arranged by size:
2385 1982 459 209 133
TatI w'GTAC_w
Cuts at: 4683 4683
Size: 5168
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4685 4717 4685
Size: 32 5136
Tth111I GACn'n_nGTC
Cuts at: 2965 2965
Size: 5168
XbaI T'CTAG_A
Cuts at: 2199 2199
Size: 5168
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2663 2663
Size: 5168
XmaI C'CCGG_G
Cuts at: 2188 2188
Size: 5168
XmnI GAAnn'nnTTC
Cuts at: 2030 2030
Size: 5168
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PasI PciI PmeI PpiI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AleI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmgBI BmtI BsiWI BsmBI
BsrGI BssHII BstXI ClaI DraIII EcoNI FseI FspAI
HpaI MfeI NdeI NheI NruI NsiI PacI PflMI
PfoI PmlI PpuMI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI