Restriction map for
pF1KB5072
(Circular) (Six-base) MAPSORT of: pF1KB5072.seq Check: 9830 from: 1 to: 4961
pF1KB5072 4961 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 1058 1999 1058
Size: 941 4020
Acc65I G'GTAC_C
Cuts at: 1977 1977
Size: 4961
AclI AA'CG_TT
Cuts at: 4801 4801
Size: 4961
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 443 913 1736 2786 3218 3965 4233 443
Size: 470 823 1050 432 747 268 1171
Fragments arranged by size:
1171 1050 823 747 470 432 268
AflIII A'CryG_T
Cuts at: 2313 3417 4394 2313
Size: 1104 977 2880
Fragments arranged by size:
2880 1104 977
AgeI A'CCGG_T
Cuts at: 3349 3349
Size: 4961
AleI CACnn'nnGTG
Cuts at: 1510 1510
Size: 4961
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 469 469
Size: 4961
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 250 282 250
Size: 32 4929
AlwNI CAG_nnn'CTG
Cuts at: 329 2266 3833 329
Size: 1937 1567 1457
Fragments arranged by size:
1937 1567 1457
ApaLI G'TGCA_C
Cuts at: 3731 3731
Size: 4961
ApoI r'AATT_y
Cuts at: 1207 1624 1965 4314 4570 1207
Size: 417 341 2349 256 1598
Fragments arranged by size:
2349 1598 417 341 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4961
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4961
AvaI C'yCGr_G
Cuts at: 472 552 752 1030 1250 1981 472
Size: 80 200 278 220 731 3452
Fragments arranged by size:
3452 731 278 220 200 80
BamHI G'GATC_C
Cuts at: 1986 1986
Size: 4961
BanI G'GyrC_C
Cuts at: 660 886 1977 2391 2639 2674 660
Size: 226 1091 414 248 35 2947
Fragments arranged by size:
2947 1091 414 248 226 35
BanII G_rGCy'C
Cuts at: 1251 1975 3005 1251
Size: 724 1030 3207
Fragments arranged by size:
3207 1030 724
BbeI G_GCGC'C
Cuts at: 2395 2643 2395
Size: 248 4713
BbsI GAAGACnn'nnnn_
Cuts at: 1094 1283 1094
Size: 189 4772
BciVI GTATCCnnnnn_n'
Cuts at: 153 2859 3620 4961 153
Size: 2706 761 1341 153
Fragments arranged by size:
2706 1341 761 153
BclI T'GATC_A
Cuts at: 1581 2481 1581
Size: 900 4061
BglI GCCn_nnn'nGGC
Cuts at: 139 139
Size: 4961
BglII A'GATC_T
Cuts at: 2476 4513 2476
Size: 2037 2924
BlpI GC'TnA_GC
Cuts at: 121 2073 121
Size: 1952 3009
Bme1580I G_kGCm'C
Cuts at: 458 665 2586 2679 3735 458
Size: 207 1921 93 1056 1684
Fragments arranged by size:
1921 1684 1056 207 93
BmgBI CAC'GTC
Cuts at: 1887 1887
Size: 4961
BmrI ACTGGGnnnn_n'
Cuts at: 2173 2254 2589 4733 2173
Size: 81 335 2144 2401
Fragments arranged by size:
2401 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3328 3328
Size: 4961
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 479 762 479
Size: 283 4678
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2137 3016 3508 3806 2137
Size: 879 492 298 3292
Fragments arranged by size:
3292 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1837 4665 1837
Size: 2828 2133
BsaAI yAC'GTr
Cuts at: 2314 2314
Size: 4961
BsaBI GATnn'nnATC
Cuts at: 6 2475 4512 6
Size: 2469 2037 455
Fragments arranged by size:
2469 2037 455
BsaHI Gr'CG_yC
Cuts at: 1091 2392 2640 3342 4838 1091
Size: 1301 248 702 1496 1214
Fragments arranged by size:
1496 1301 1214 702 248
BsaWI w'CCGG_w
Cuts at: 2150 2671 3349 3623 3770 4181 2150
Size: 521 678 274 147 411 2930
Fragments arranged by size:
2930 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1238 1268 1238
Size: 30 4931
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 482 482
Size: 4961
BseYI C'CCAG_C
Cuts at: 513 533 619 2386 3144 3721 4217 513
Size: 20 86 1767 758 577 496 1257
Fragments arranged by size:
1767 1257 758 577 496 86 20
BsiEI CG_ry'CG
Cuts at: 104 2503 2549 3757 104
Size: 2399 46 1208 1308
Fragments arranged by size:
2399 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 711 1975 2753 2943 3735 711
Size: 1264 778 190 792 1937
Fragments arranged by size:
1937 1264 792 778 190
BsmI GAATG_Cn'
Cuts at: 1803 1803
Size: 4961
Bsp1286I G_dGCh'C
Cuts at: 458 665 711 1251 1975 2586 2679 2753 2943
Size: 207 46 540 724 611 93 74 190
Cuts at: 2943 3005 3735 458
Size: 62 730 1684
Fragments arranged by size:
1684 730 724 611 540 207 190 93
74 62 46
BspEI T'CCGG_A
Cuts at: 4181 4181
Size: 4961
BspHI T'CATG_A
Cuts at: 242 1928 4137 242
Size: 1686 2209 1066
Fragments arranged by size:
2209 1686 1066
BspMI ACCTGCnnnn'nnnn_
Cuts at: 604 2011 2225 2527 2908 604
Size: 1407 214 302 381 2657
Fragments arranged by size:
2657 1407 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1403 3253 3307 4791 4956 50
Size: 1353 1850 54 1484 165 55
Fragments arranged by size:
1850 1484 1353 165 55 54
BsrDI GCAATG_nn'
Cuts at: 134 665 1304 2873 134
Size: 531 639 1569 2222
Fragments arranged by size:
2222 1569 639 531
BsrFI r'CCGG_y
Cuts at: 2959 3140 3349 2959
Size: 181 209 4571
Fragments arranged by size:
4571 209 181
BssSI C'ACGA_G
Cuts at: 301 710 3232 3590 301
Size: 409 2522 358 1672
Fragments arranged by size:
2522 1672 409 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2338 2338
Size: 4961
BstBI TT'CG_AA
Cuts at: 3322 3322
Size: 4961
BstXI CCAn_nnnn'nTGG
Cuts at: 1881 1881
Size: 4961
BstYI r'GATC_y
Cuts at: 1 731 1070 1986 2453 2476 2811 3057 4069
Size: 730 339 916 467 23 335 246 1012
Cuts at: 4069 4155 4167 4513 1
Size: 86 12 346 449
Fragments arranged by size:
1012 916 730 467 449 346 339 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 1895 107
Size: 1788 3173
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2911 3092 3255 2911
Size: 181 163 4617
Fragments arranged by size:
4617 181 163
BtsI GCAGTG_nn'
Cuts at: 2204 2204
Size: 4961
DraI TTT'AAA
Cuts at: 1960 1960
Size: 4961
DrdI GACnn_nn'nnGTC
Cuts at: 2667 3525 2667
Size: 858 4103
EaeI y'GGCC_r
Cuts at: 114 140 1502 1914 2546 2720 3111 3138 4483
Size: 26 1362 412 632 174 391 27 1345
Cuts at: 4483 114
Size: 592
Fragments arranged by size:
1362 1345 632 592 412 391 174 27
26
EagI C'GGCC_G
Cuts at: 2546 2546
Size: 4961
EarI CTCTTCn'nnn_
Cuts at: 2984 3194 2984
Size: 210 4751
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 212 1421 3169 3479 3625 4771 212
Size: 1209 1748 310 146 1146 402
Fragments arranged by size:
1748 1209 1146 402 310 146
EcoICRI GAG'CTC
Cuts at: 1973 1973
Size: 4961
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 443 479 762 913 1736 2786 3218 3965 4233
Size: 36 283 151 823 1050 432 747 268
Cuts at: 4233 443
Size: 1171
Fragments arranged by size:
1171 1050 823 747 432 283 268 151
36
EcoO109I rG'GnC_Cy
Cuts at: 530 616 2100 530
Size: 86 1484 3391
Fragments arranged by size:
3391 1484 86
EcoRI G'AATT_C
Cuts at: 1965 1965
Size: 4961
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 784 1069 1741 2980 784
Size: 285 672 1239 2765
Fragments arranged by size:
2765 1239 672 285
FspI TGC'GCA
Cuts at: 2742 2742
Size: 4961
HaeII r_GCGC'y
Cuts at: 502 2395 2643 3665 4644 502
Size: 1893 248 1022 979 819
Fragments arranged by size:
1893 1022 979 819 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 374 406 432 464 1024 1056 1238 1262 1270
Size: 32 26 32 560 32 182 24 8
Cuts at: 1270 1294 1438 1470 1817 1849 2803 2833 2835
Size: 24 144 32 347 32 954 30 2
Cuts at: 2835 2865 374
Size: 30 2470
Fragments arranged by size:
2470 954 560 347 182 144 32 32
32 32 32 30 30 26 24 24
8 2
HincII GTy'rAC
Cuts at: 789 2000 789
Size: 1211 3750
HindIII A'AGCT_T
Cuts at: 766 1336 766
Size: 570 4391
KasI G'GCGC_C
Cuts at: 2391 2639 2391
Size: 248 4713
KpnI G_GTAC'C
Cuts at: 1981 1981
Size: 4961
MfeI C'AATT_G
Cuts at: 316 316
Size: 4961
MluI A'CGCG_T
Cuts at: 4394 4394
Size: 4961
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 411 1392 3632 3816 411
Size: 981 2240 184 1556
Fragments arranged by size:
2240 1556 981 184
MscI TGG'CCA
Cuts at: 1504 2722 1504
Size: 1218 3743
MslI CAynn'nnrTG
Cuts at: 1510 1879 2950 3077 1510
Size: 369 1071 127 3394
Fragments arranged by size:
3394 1071 369 127
MspA1I CmG'CkG
Cuts at: 120 421 919 1604 2072 2386 2746 3759 4004
Size: 301 498 685 468 314 360 1013 245
Cuts at: 4004 120
Size: 1077
Fragments arranged by size:
1077 1013 685 498 468 360 314 301
245
NaeI GCC'GGC
Cuts at: 3142 3142
Size: 4961
NarI GG'CG_CC
Cuts at: 2392 2640 2392
Size: 248 4713
NcoI C'CATG_G
Cuts at: 107 107
Size: 4961
NgoMIV G'CCGG_C
Cuts at: 3140 3140
Size: 4961
NspI r_CATG'y
Cuts at: 1539 2014 2514 3421 1539
Size: 475 500 907 3079
Fragments arranged by size:
3079 907 500 475
PciI A'CATG_T
Cuts at: 3417 3417
Size: 4961
PfoI T'CCnGG_A
Cuts at: 1162 1703 1162
Size: 541 4420
PmeI GTTT'AAAC
Cuts at: 1960 1960
Size: 4961
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4100 4132 4100
Size: 32 4929
PpuMI rG'GwC_Cy
Cuts at: 616 616
Size: 4961
PshAI GACnn'nnGTC
Cuts at: 961 961
Size: 4961
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1522 1554 1522
Size: 32 4929
PstI C_TGCA'G
Cuts at: 615 2008 2693 615
Size: 1393 685 2883
Fragments arranged by size:
2883 1393 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4961
PvuII CAG'CTG
Cuts at: 120 421 919 2386 2746 120
Size: 301 498 1467 360 2335
Fragments arranged by size:
2335 1467 498 360 301
RsrII CG'GwC_CG
Cuts at: 3156 3156
Size: 4961
SacI G_AGCT'C
Cuts at: 1975 1975
Size: 4961
SalI G'TCGA_C
Cuts at: 1998 1998
Size: 4961
SapI GCTCTTCn'nnn_
Cuts at: 2984 3194 2984
Size: 210 4751
SbfI CC_TGCA'GG
Cuts at: 2008 2008
Size: 4961
ScaI AGT'ACT
Cuts at: 4478 4478
Size: 4961
SfcI C'TryA_G
Cuts at: 33 611 812 1902 2004 2689 3682 3873 33
Size: 578 201 1090 102 685 993 191 1121
Fragments arranged by size:
1121 1090 993 685 578 201 191 102
SfiI GGCCn_nnn'nGGCC
Cuts at: 139 139
Size: 4961
SfoI GGC'GCC
Cuts at: 2393 2641 2393
Size: 248 4713
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4961
SmaI CCC'GGG
Cuts at: 1983 1983
Size: 4961
SmlI C'TyrA_G
Cuts at: 1030 2116 3031 3523 3785 1030
Size: 1086 915 492 262 2206
Fragments arranged by size:
2206 1086 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4961
SphI G_CATG'C
Cuts at: 2014 2514 2014
Size: 500 4461
SspI AAT'ATT
Cuts at: 1665 1665
Size: 4961
StuI AGG'CCT
Cuts at: 1330 1330
Size: 4961
StyI C'CwwG_G
Cuts at: 107 1470 1725 2095 2449 107
Size: 1363 255 370 354 2619
Fragments arranged by size:
2619 1363 370 354 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1680 2544 2677 3136 3345 1680
Size: 864 133 459 209 3296
Fragments arranged by size:
3296 864 459 209 133
TatI w'GTAC_w
Cuts at: 4476 4476
Size: 4961
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 690 722 1611 1643 4478 4510 690
Size: 32 889 32 2835 32 1141
Fragments arranged by size:
2835 1141 889 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2758 2758
Size: 4961
XbaI T'CTAG_A
Cuts at: 1219 1992 1219
Size: 773 4188
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 541 2456 541
Size: 1915 3046
XhoI C'TCGA_G
Cuts at: 1030 1030
Size: 4961
XmaI C'CCGG_G
Cuts at: 1981 1981
Size: 4961
XmnI GAAnn'nnTTC
Cuts at: 561 769 561
Size: 208 4753
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PpiI PpuMI PshAI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsgI BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PsiI
PspOMI PspXI SacII SanDI SexAI SgrAI SnaBI SrfI
SwaI ZraI