Restriction map for
pF1KB5068
(Circular) (Six-base) MAPSORT of: pF1KB5068.seq Check: 7771 from: 1 to: 4754
pF1KB5068 4754 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1126 1126
Size: 4754
AccI GT'mk_AC
Cuts at: 1792 1792
Size: 4754
Acc65I G'GTAC_C
Cuts at: 1770 1770
Size: 4754
AclI AA'CG_TT
Cuts at: 4594 4594
Size: 4754
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 148 491 1079 2579 3011 3364 4026 140
Size: 8 343 588 1500 432 353 662 868
Fragments arranged by size:
1500 868 662 588 432 353 343 8
AflII C'TTAA_G
Cuts at: 760 1486 760
Size: 726 4028
AflIII A'CryG_T
Cuts at: 2106 3906 4187 2106
Size: 1800 281 2673
Fragments arranged by size:
2673 1800 281
AleI CACnn'nnGTG
Cuts at: 964 964
Size: 4754
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 907 907
Size: 4754
AlwNI CAG_nnn'CTG
Cuts at: 420 469 1527 2059 3497 420
Size: 49 1058 532 1438 1677
Fragments arranged by size:
1677 1438 1058 532 49
ApaI G_GGCC'C
Cuts at: 261 1074 1106 1339 261
Size: 813 32 233 3676
Fragments arranged by size:
3676 813 233 32
ApaLI G'TGCA_C
Cuts at: 3592 3592
Size: 4754
ApoI r'AATT_y
Cuts at: 838 1723 1758 4107 4363 838
Size: 885 35 2349 256 1229
Fragments arranged by size:
2349 1229 885 256 35
AseI AT'TA_AT
Cuts at: 20 20
Size: 4754
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4754
AvaI C'yCGr_G
Cuts at: 254 437 1145 1774 254
Size: 183 708 629 3234
Fragments arranged by size:
3234 708 629 183
AvrII C'CTAG_G
Cuts at: 1510 1510
Size: 4754
BamHI G'GATC_C
Cuts at: 1779 1779
Size: 4754
BanI G'GyrC_C
Cuts at: 998 1770 2184 2432 2467 998
Size: 772 414 248 35 3285
Fragments arranged by size:
3285 772 414 248 35
BanII G_rGCy'C
Cuts at: 261 271 647 1074 1106 1339 1768 2798 261
Size: 10 376 427 32 233 429 1030 2217
Fragments arranged by size:
2217 1030 429 427 376 233 32 10
BbeI G_GCGC'C
Cuts at: 2188 2436 2188
Size: 248 4506
BbsI GAAGACnn'nnnn_
Cuts at: 283 479 839 1508 1643 283
Size: 196 360 669 135 3394
Fragments arranged by size:
3394 669 360 196 135
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1414 1448 1414
Size: 34 4720
BciVI GTATCCnnnnn_n'
Cuts at: 2652 3708 4754 2652
Size: 1056 1046 2652
Fragments arranged by size:
2652 1056 1046
BclI T'GATC_A
Cuts at: 2274 2274
Size: 4754
BglII A'GATC_T
Cuts at: 2269 4306 2269
Size: 2037 2717
BlpI GC'TnA_GC
Cuts at: 1866 1866
Size: 4754
Bme1580I G_kGCm'C
Cuts at: 261 1074 1106 1339 2379 2472 3596 261
Size: 813 32 233 1040 93 1124 1419
Fragments arranged by size:
1419 1124 1040 813 233 93 32
BmrI ACTGGGnnnn_n'
Cuts at: 136 1069 1966 2047 2382 4526 136
Size: 933 897 81 335 2144 364
Fragments arranged by size:
2144 933 897 364 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3121 3121
Size: 4754
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 978 1504 607
Size: 371 526 3857
Fragments arranged by size:
3857 526 371
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 631 712 847 1930 2809 3523 3821 631
Size: 81 135 1083 879 714 298 1564
Fragments arranged by size:
1564 1083 879 714 298 135 81
BsaI GGTCTCn'nnnn_
Cuts at: 4458 4458
Size: 4754
BsaAI yAC'GTr
Cuts at: 2107 2107
Size: 4754
BsaBI GATnn'nnATC
Cuts at: 6 2268 4305 6
Size: 2262 2037 455
Fragments arranged by size:
2262 2037 455
BsaHI Gr'CG_yC
Cuts at: 2185 2433 3135 4631 2185
Size: 248 702 1496 2308
Fragments arranged by size:
2308 1496 702 248
BsaWI w'CCGG_w
Cuts at: 336 1128 1361 1943 2464 3142 3553 3700 3974
Size: 792 233 582 521 678 411 147 274
Cuts at: 3974 336
Size: 1116
Fragments arranged by size:
1116 792 678 582 521 411 274 233
147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 646 1324 1384 646
Size: 678 60 4016
Fragments arranged by size:
4016 678 60
BseYI C'CCAG_C
Cuts at: 776 2179 2937 3602 4010 776
Size: 1403 758 665 408 1520
Fragments arranged by size:
1520 1403 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 284 2296 2342 3572 104
Size: 180 2012 46 1230 1286
Fragments arranged by size:
2012 1286 1230 180 46
BsiHKAI G_wGCw'C
Cuts at: 438 647 971 1181 1768 2546 2736 3596 438
Size: 209 324 210 587 778 190 860 1596
Fragments arranged by size:
1596 860 778 587 324 210 209 190
BsmI GAATG_Cn'
Cuts at: 1473 1473
Size: 4754
Bsp1286I G_dGCh'C
Cuts at: 261 271 438 647 971 1074 1106 1181 1339
Size: 10 167 209 324 103 32 75 158
Cuts at: 1339 1768 2379 2472 2546 2736 2798 3596 261
Size: 429 611 93 74 190 62 798 1419
Fragments arranged by size:
1419 798 611 429 324 209 190 167
158 103 93 75 74 62 32 10
BspEI T'CCGG_A
Cuts at: 1128 1361 1128
Size: 233 4521
BspHI T'CATG_A
Cuts at: 3186 3186
Size: 4754
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1126 1804 2018 2320 2701 1126
Size: 678 214 302 381 3179
Fragments arranged by size:
3179 678 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3046 3100 4584 4749 50
Size: 2996 54 1484 165 55
Fragments arranged by size:
2996 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2666 2666
Size: 4754
BsrFI r'CCGG_y
Cuts at: 923 2752 2933 923
Size: 1829 181 2744
Fragments arranged by size:
2744 1829 181
BssSI C'ACGA_G
Cuts at: 3025 3733 3025
Size: 708 4046
BstAPI GCAn_nnn'nTGC
Cuts at: 312 1054 2131 312
Size: 742 1077 2935
Fragments arranged by size:
2935 1077 742
BstBI TT'CG_AA
Cuts at: 134 3115 134
Size: 2981 1773
BstEII G'GTnAC_C
Cuts at: 753 753
Size: 4754
BstXI CCAn_nnnn'nTGG
Cuts at: 225 387 784 1225 225
Size: 162 397 441 3754
Fragments arranged by size:
3754 441 397 162
BstYI r'GATC_y
Cuts at: 1 1779 2246 2269 2604 2850 3156 3168 3254
Size: 1778 467 23 335 246 306 12 86
Cuts at: 3254 4306 1
Size: 1052 449
Fragments arranged by size:
1778 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1205 1205
Size: 4754
BtgI C'CryG_G
Cuts at: 107 1280 1611 107
Size: 1173 331 3250
Fragments arranged by size:
3250 1173 331
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2704 2885 3048 2704
Size: 181 163 4410
Fragments arranged by size:
4410 181 163
BtsI GCAGTG_nn'
Cuts at: 359 985 1562 1693 1997 359
Size: 626 577 131 304 3116
Fragments arranged by size:
3116 626 577 304 131
DraI TTT'AAA
Cuts at: 1753 1753
Size: 4754
DraIII CAC_nnn'GTG
Cuts at: 1167 1167
Size: 4754
DrdI GACnn_nn'nnGTC
Cuts at: 2460 3804 2460
Size: 1344 3410
EaeI y'GGCC_r
Cuts at: 281 452 2339 2513 2904 2931 4276 281
Size: 171 1887 174 391 27 1345 759
Fragments arranged by size:
1887 1345 759 391 174 171 27
EagI C'GGCC_G
Cuts at: 281 2339 281
Size: 2058 2696
EarI CTCTTCn'nnn_
Cuts at: 635 1457 2777 2987 635
Size: 822 1320 210 2402
Fragments arranged by size:
2402 1320 822 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2962 3704 3850 4564 2962
Size: 742 146 714 3152
Fragments arranged by size:
3152 742 714 146
EcoICRI GAG'CTC
Cuts at: 645 1766 645
Size: 1121 3633
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 148 491 607 978 1079 1504 2579 3011
Size: 8 343 116 371 101 425 1075 432
Cuts at: 3011 3364 4026 140
Size: 353 662 868
Fragments arranged by size:
1075 868 662 432 425 371 353 343
116 101 8
EcoNI CCTnn'n_nnAGG
Cuts at: 1659 1659
Size: 4754
EcoO109I rG'GnC_Cy
Cuts at: 243 527 1070 1071 1102 1335 1336 1515 1893
Size: 284 543 1 31 233 1 179 378
Cuts at: 1893 243
Size: 3104
Fragments arranged by size:
3104 543 378 284 233 179 31 1
1
EcoRI G'AATT_C
Cuts at: 1758 1758
Size: 4754
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 631 1512 2773 631
Size: 881 1261 2612
Fragments arranged by size:
2612 1261 881
FspI TGC'GCA
Cuts at: 1699 2535 1699
Size: 836 3918
HaeII r_GCGC'y
Cuts at: 2188 2436 3666 4437 2188
Size: 248 1230 771 2505
Fragments arranged by size:
2505 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 466 498 519 551 1105 1137 2596 2626 2628
Size: 32 21 32 554 32 1459 30 2
Cuts at: 2628 2658 466
Size: 30 2562
Fragments arranged by size:
2562 1459 554 32 32 32 30 30
21 2
HincII GTy'rAC
Cuts at: 903 1793 903
Size: 890 3864
HindIII A'AGCT_T
Cuts at: 1483 1483
Size: 4754
KasI G'GCGC_C
Cuts at: 2184 2432 2184
Size: 248 4506
KpnI G_GTAC'C
Cuts at: 1774 1774
Size: 4754
MluI A'CGCG_T
Cuts at: 4187 4187
Size: 4754
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3513 3697 3513
Size: 184 4570
MscI TGG'CCA
Cuts at: 454 2515 454
Size: 2061 2693
MslI CAynn'nnrTG
Cuts at: 964 2743 2870 964
Size: 1779 127 2848
Fragments arranged by size:
2848 1779 127
MspA1I CmG'CkG
Cuts at: 309 420 466 780 1524 1865 2179 2539 3323
Size: 111 46 314 744 341 314 360 784
Cuts at: 3323 3568 309
Size: 245 1495
Fragments arranged by size:
1495 784 744 360 341 314 314 245
111 46
NaeI GCC'GGC
Cuts at: 2935 2935
Size: 4754
NarI GG'CG_CC
Cuts at: 2185 2433 2185
Size: 248 4506
NcoI C'CATG_G
Cuts at: 107 107
Size: 4754
NgoMIV G'CCGG_C
Cuts at: 2933 2933
Size: 4754
NruI TCG'CGA
Cuts at: 192 192
Size: 4754
NspI r_CATG'y
Cuts at: 1807 2307 3910 1807
Size: 500 1603 2651
Fragments arranged by size:
2651 1603 500
PciI A'CATG_T
Cuts at: 3906 3906
Size: 4754
PfoI T'CCnGG_A
Cuts at: 206 271 206
Size: 65 4689
PmeI GTTT'AAAC
Cuts at: 1753 1753
Size: 4754
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 350 382 3200 3232 350
Size: 32 2818 32 1872
Fragments arranged by size:
2818 1872 32 32
PpuMI rG'GwC_Cy
Cuts at: 243 527 1515 243
Size: 284 988 3482
Fragments arranged by size:
3482 988 284
PspOMI G'GGCC_C
Cuts at: 257 1070 1102 1335 257
Size: 813 32 233 3676
Fragments arranged by size:
3676 813 233 32
PstI C_TGCA'G
Cuts at: 308 1399 1801 2486 308
Size: 1091 402 685 2576
Fragments arranged by size:
2576 1091 685 402
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4754
PvuII CAG'CTG
Cuts at: 309 420 466 780 1524 2179 2539 309
Size: 111 46 314 744 655 360 2524
Fragments arranged by size:
2524 744 655 360 314 111 46
RsrII CG'GwC_CG
Cuts at: 2949 2949
Size: 4754
SacI G_AGCT'C
Cuts at: 647 1768 647
Size: 1121 3633
SalI G'TCGA_C
Cuts at: 1791 1791
Size: 4754
SanDI GG'GwC_CC
Cuts at: 527 527
Size: 4754
SapI GCTCTTCn'nnn_
Cuts at: 635 1457 2777 2987 635
Size: 822 1320 210 2402
Fragments arranged by size:
2402 1320 822 210
SbfI CC_TGCA'GG
Cuts at: 1801 1801
Size: 4754
ScaI AGT'ACT
Cuts at: 4271 4271
Size: 4754
SexAI A'CCwGG_T
Cuts at: 1592 1592
Size: 4754
SfcI C'TryA_G
Cuts at: 33 304 1395 1797 2482 3450 3641 33
Size: 271 1091 402 685 968 191 1146
Fragments arranged by size:
1146 1091 968 685 402 271 191
SfoI GGC'GCC
Cuts at: 2186 2434 2186
Size: 248 4506
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4754
SmaI CCC'GGG
Cuts at: 1147 1776 1147
Size: 629 4125
SmlI C'TyrA_G
Cuts at: 646 727 760 862 1486 1909 2824 3538 3800
Size: 81 33 102 624 423 915 714 262
Cuts at: 3800 646
Size: 1600
Fragments arranged by size:
1600 915 714 624 423 262 102 81
33
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4754
SphI G_CATG'C
Cuts at: 1807 2307 1807
Size: 500 4254
StuI AGG'CCT
Cuts at: 362 1120 1210 362
Size: 758 90 3906
Fragments arranged by size:
3906 758 90
StyI C'CwwG_G
Cuts at: 107 380 1023 1510 1888 2242 107
Size: 273 643 487 378 354 2619
Fragments arranged by size:
2619 643 487 378 354 273
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2337 2470 2929 3138 2337
Size: 133 459 209 3953
Fragments arranged by size:
3953 459 209 133
TatI w'GTAC_w
Cuts at: 4269 4269
Size: 4754
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4271 4303 4271
Size: 32 4722
Tth111I GACn'n_nGTC
Cuts at: 478 1039 2551 478
Size: 561 1512 2681
Fragments arranged by size:
2681 1512 561
XbaI T'CTAG_A
Cuts at: 909 1785 909
Size: 876 3878
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2249 2249
Size: 4754
XmaI C'CCGG_G
Cuts at: 1145 1774 1145
Size: 629 4125
XmnI GAAnn'nnTTC
Cuts at: 1466 1466
Size: 4754
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PciI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AloI AscI BaeI BbvCI
BfrBI BglI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI
BsmBI BsrGI BssHII BstZ17I ClaI CspCI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NsiI PacI
PasI PflMI PmlI PshAI PsiI PspXI PsrI SacII
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI