Restriction map for
pF1KB5060
(Circular) (Six-base) MAPSORT of: pF1KB5060.seq Check: 891 from: 1 to: 4868
pF1KB5060 4868 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AatII G_ACGT'C
Cuts at: 1709 1709
Size: 4868
AccI GT'mk_AC
Cuts at: 1550 1906 1550
Size: 356 4512
Acc65I G'GTAC_C
Cuts at: 1884 1884
Size: 4868
AclI AA'CG_TT
Cuts at: 4708 4708
Size: 4868
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 1093 2693 3125 3478 4140 454
Size: 639 1600 432 353 662 1182
Fragments arranged by size:
1600 1182 662 639 432 353
AflIII A'CryG_T
Cuts at: 2220 4020 4301 2220
Size: 1800 281 2787
Fragments arranged by size:
2787 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 417 417
Size: 4868
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 859 891 859
Size: 32 4836
AlwNI CAG_nnn'CTG
Cuts at: 327 660 954 1248 1278 1373 2173 3611 327
Size: 333 294 294 30 95 800 1438 1584
Fragments arranged by size:
1584 1438 800 333 294 294 95 30
ApaI G_GGCC'C
Cuts at: 300 300
Size: 4868
ApaLI G'TGCA_C
Cuts at: 384 1379 3706 384
Size: 995 2327 1546
Fragments arranged by size:
2327 1546 995
ApoI r'AATT_y
Cuts at: 1252 1872 4221 4477 1252
Size: 620 2349 256 1643
Fragments arranged by size:
2349 1643 620 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4868
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4868
AvaI C'yCGr_G
Cuts at: 1888 1888
Size: 4868
BamHI G'GATC_C
Cuts at: 1893 1893
Size: 4868
BanI G'GyrC_C
Cuts at: 196 1050 1884 2298 2546 2581 196
Size: 854 834 414 248 35 2483
Fragments arranged by size:
2483 854 834 414 248 35
BanII G_rGCy'C
Cuts at: 300 524 1175 1610 1694 1882 2912 300
Size: 224 651 435 84 188 1030 2256
Fragments arranged by size:
2256 1030 651 435 224 188 84
BbeI G_GCGC'C
Cuts at: 200 2302 2550 200
Size: 2102 248 2518
Fragments arranged by size:
2518 2102 248
BbsI GAAGACnn'nnnn_
Cuts at: 1240 1240
Size: 4868
BciVI GTATCCnnnnn_n'
Cuts at: 1305 2766 3822 4868 1305
Size: 1461 1056 1046 1305
Fragments arranged by size:
1461 1305 1056 1046
BclI T'GATC_A
Cuts at: 2388 2388
Size: 4868
BglI GCCn_nnn'nGGC
Cuts at: 242 322 406 560 811 242
Size: 80 84 154 251 4299
Fragments arranged by size:
4299 251 154 84 80
BglII A'GATC_T
Cuts at: 221 542 2383 4420 221
Size: 321 1841 2037 669
Fragments arranged by size:
2037 1841 669 321
BlpI GC'TnA_GC
Cuts at: 1980 1980
Size: 4868
Bme1580I G_kGCm'C
Cuts at: 247 300 388 716 1055 1268 1383 1749 1802
Size: 53 88 328 339 213 115 366 53
Cuts at: 1802 2493 2586 3710 247
Size: 691 93 1124 1405
Fragments arranged by size:
1405 1124 691 366 339 328 213 115
93 88 53 53
BmrI ACTGGGnnnn_n'
Cuts at: 992 1736 2080 2161 2496 4640 992
Size: 744 344 81 335 2144 1220
Fragments arranged by size:
2144 1220 744 344 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1125 3235 1125
Size: 2110 2758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 498 667 952 1110 1227 1368 1395 498
Size: 169 285 158 117 141 27 3971
Fragments arranged by size:
3971 285 169 158 141 117 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2044 2923 3637 3935 2044
Size: 879 714 298 2977
Fragments arranged by size:
2977 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4572 4572
Size: 4868
BsaAI yAC'GTr
Cuts at: 2221 2221
Size: 4868
BsaBI GATnn'nnATC
Cuts at: 6 760 2382 4419 6
Size: 754 1622 2037 455
Fragments arranged by size:
2037 1622 754 455
BsaHI Gr'CG_yC
Cuts at: 197 602 923 1706 2299 2547 3249 4745 197
Size: 405 321 783 593 248 702 1496 320
Fragments arranged by size:
1496 783 702 593 405 321 320 248
BsaWI w'CCGG_w
Cuts at: 2057 2578 3256 3667 3814 4088 2057
Size: 521 678 411 147 274 2837
Fragments arranged by size:
2837 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 730 760 1573 1603 730
Size: 30 813 30 3995
Fragments arranged by size:
3995 813 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 847 1132 1183 1587 1767 1839 847
Size: 285 51 404 180 72 3876
Fragments arranged by size:
3876 404 285 180 72 51
BseYI C'CCAG_C
Cuts at: 1054 1094 1507 2293 3051 3716 4124 1054
Size: 40 413 786 758 665 408 1798
Fragments arranged by size:
1798 786 758 665 413 408 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 459 610 459
Size: 151 4717
BsiEI CG_ry'CG
Cuts at: 104 2410 2456 3686 104
Size: 2306 46 1230 1286
Fragments arranged by size:
2306 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 388 496 524 1175 1383 1610 1774 1882 2660
Size: 108 28 651 208 227 164 108 778
Cuts at: 2660 2850 3710 388
Size: 190 860 1546
Fragments arranged by size:
1546 860 778 651 227 208 190 164
108 108 28
BsmBI CGTCTCn'nnnn_
Cuts at: 452 452
Size: 4868
Bsp1286I G_dGCh'C
Cuts at: 247 300 388 496 524 716 1055 1175 1268
Size: 53 88 108 28 192 339 120 93
Cuts at: 1268 1383 1610 1694 1749 1774 1802 1882 2493
Size: 115 227 84 55 25 28 80 611
Cuts at: 2493 2586 2660 2850 2912 3710 247
Size: 93 74 190 62 798 1405
Fragments arranged by size:
1405 798 611 339 227 192 190 120
115 108 93 93 88 84 80 74
62 55 53 28 28 25
BspHI T'CATG_A
Cuts at: 3300 3300
Size: 4868
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1022 1918 2132 2434 2815 1022
Size: 896 214 302 381 3075
Fragments arranged by size:
3075 896 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3160 3214 4698 4863 50
Size: 3110 54 1484 165 55
Fragments arranged by size:
3110 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 687 1652 2780 687
Size: 965 1128 2775
Fragments arranged by size:
2775 1128 965
BsrFI r'CCGG_y
Cuts at: 2866 3047 2866
Size: 181 4687
BssSI C'ACGA_G
Cuts at: 451 1213 3139 3847 451
Size: 762 1926 708 1472
Fragments arranged by size:
1926 1472 762 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2245 2245
Size: 4868
BstBI TT'CG_AA
Cuts at: 3229 3229
Size: 4868
BstXI CCAn_nnnn'nTGG
Cuts at: 369 696 1062 1749 369
Size: 327 366 687 3488
Fragments arranged by size:
3488 687 366 327
BstYI r'GATC_y
Cuts at: 1 221 542 1142 1893 2360 2383 2718 2964
Size: 220 321 600 751 467 23 335 246
Cuts at: 2964 3270 3282 3368 4420 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 751 600 467 449 335 321 306
246 220 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1166 1851 1166
Size: 685 4183
BtgI C'CryG_G
Cuts at: 716 1079 716
Size: 363 4505
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2818 2999 3162 2818
Size: 181 163 4524
Fragments arranged by size:
4524 181 163
BtsI GCAGTG_nn'
Cuts at: 914 2111 914
Size: 1197 3671
DraI TTT'AAA
Cuts at: 1867 1867
Size: 4868
DraIII CAC_nnn'GTG
Cuts at: 251 1263 251
Size: 1012 3856
DrdI GACnn_nn'nnGTC
Cuts at: 2574 3918 2574
Size: 1344 3524
EaeI y'GGCC_r
Cuts at: 185 407 537 618 911 2453 2627 3018 3045
Size: 222 130 81 293 1542 174 391 27
Cuts at: 3045 4390 185
Size: 1345 663
Fragments arranged by size:
1542 1345 663 391 293 222 174 130
81 27
EagI C'GGCC_G
Cuts at: 2453 2453
Size: 4868
EarI CTCTTCn'nnn_
Cuts at: 130 430 1522 1699 1762 2891 3101 130
Size: 300 1092 177 63 1129 210 1897
Fragments arranged by size:
1897 1129 1092 300 210 177 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1816 3076 3818 3964 4678 1816
Size: 1260 742 146 714 2006
Fragments arranged by size:
2006 1260 742 714 146
EcoICRI GAG'CTC
Cuts at: 522 1173 1608 1880 522
Size: 651 435 272 3510
Fragments arranged by size:
3510 651 435 272
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 498 667 952 1093 1110 1227 1368 1395
Size: 44 169 285 141 17 117 141 27
Cuts at: 1395 2693 3125 3478 4140 454
Size: 1298 432 353 662 1182
Fragments arranged by size:
1298 1182 662 432 353 285 169 141
141 117 44 27 17
EcoNI CCTnn'n_nnAGG
Cuts at: 664 862 1072 1306 1519 664
Size: 198 210 234 213 4013
Fragments arranged by size:
4013 234 213 210 198
EcoO109I rG'GnC_Cy
Cuts at: 267 297 358 531 657 726 867 1218 1245
Size: 30 61 173 126 69 141 351 27
Cuts at: 1245 1417 2007 267
Size: 172 590 3128
Fragments arranged by size:
3128 590 351 173 172 141 126 69
61 30 27
EcoRI G'AATT_C
Cuts at: 1252 1872 1252
Size: 620 4248
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1164 2887 1164
Size: 1723 3145
FspI TGC'GCA
Cuts at: 837 2649 837
Size: 1812 3056
HaeII r_GCGC'y
Cuts at: 200 2302 2550 3780 4551 200
Size: 2102 248 1230 771 517
Fragments arranged by size:
2102 1230 771 517 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 403 435 2710 2740 2742 2772 403
Size: 32 2275 30 2 30 2499
Fragments arranged by size:
2499 2275 32 30 30 2
HincII GTy'rAC
Cuts at: 1907 1907
Size: 4868
KasI G'GCGC_C
Cuts at: 196 2298 2546 196
Size: 2102 248 2518
Fragments arranged by size:
2518 2102 248
KpnI G_GTAC'C
Cuts at: 1888 1888
Size: 4868
MluI A'CGCG_T
Cuts at: 4301 4301
Size: 4868
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1044 3627 3811 1044
Size: 2583 184 2101
Fragments arranged by size:
2583 2101 184
MscI TGG'CCA
Cuts at: 539 620 913 2629 539
Size: 81 293 1716 2778
Fragments arranged by size:
2778 1716 293 81
MslI CAynn'nnrTG
Cuts at: 580 694 2857 2984 580
Size: 114 2163 127 2464
Fragments arranged by size:
2464 2163 127 114
MspA1I CmG'CkG
Cuts at: 558 822 1979 2293 2653 3437 3682 558
Size: 264 1157 314 360 784 245 1744
Fragments arranged by size:
1744 1157 784 360 314 264 245
NaeI GCC'GGC
Cuts at: 3049 3049
Size: 4868
NarI GG'CG_CC
Cuts at: 197 2299 2547 197
Size: 2102 248 2518
Fragments arranged by size:
2518 2102 248
NcoI C'CATG_G
Cuts at: 716 716
Size: 4868
NgoMIV G'CCGG_C
Cuts at: 3047 3047
Size: 4868
NspI r_CATG'y
Cuts at: 180 1921 2421 4024 180
Size: 1741 500 1603 1024
Fragments arranged by size:
1741 1603 1024 500
PasI CC'CwG_GG
Cuts at: 300 423 606 300
Size: 123 183 4562
Fragments arranged by size:
4562 183 123
PciI A'CATG_T
Cuts at: 4020 4020
Size: 4868
PflMI CCAn_nnn'nTGG
Cuts at: 327 504 795 1278 327
Size: 177 291 483 3917
Fragments arranged by size:
3917 483 291 177
PfoI T'CCnGG_A
Cuts at: 1372 1520 1372
Size: 148 4720
PmeI GTTT'AAAC
Cuts at: 1867 1867
Size: 4868
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1171 1203 1207 1239 3314 3346 1171
Size: 32 4 32 2075 32 2693
Fragments arranged by size:
2693 2075 32 32 32 4
PpuMI rG'GwC_Cy
Cuts at: 267 726 867 267
Size: 459 141 4268
Fragments arranged by size:
4268 459 141
PspOMI G'GGCC_C
Cuts at: 296 296
Size: 4868
PstI C_TGCA'G
Cuts at: 448 827 1187 1244 1915 2600 448
Size: 379 360 57 671 685 2716
Fragments arranged by size:
2716 685 671 379 360 57
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4868
PvuII CAG'CTG
Cuts at: 822 2293 2653 822
Size: 1471 360 3037
Fragments arranged by size:
3037 1471 360
RsrII CG'GwC_CG
Cuts at: 3063 3063
Size: 4868
SacI G_AGCT'C
Cuts at: 524 1175 1610 1882 524
Size: 651 435 272 3510
Fragments arranged by size:
3510 651 435 272
SalI G'TCGA_C
Cuts at: 1905 1905
Size: 4868
SapI GCTCTTCn'nnn_
Cuts at: 130 430 1699 1762 2891 3101 130
Size: 300 1269 63 1129 210 1897
Fragments arranged by size:
1897 1269 1129 300 210 63
SbfI CC_TGCA'GG
Cuts at: 1915 1915
Size: 4868
ScaI AGT'ACT
Cuts at: 4385 4385
Size: 4868
SfcI C'TryA_G
Cuts at: 33 228 444 823 946 1183 1240 1464 1911
Size: 195 216 379 123 237 57 224 447
Cuts at: 1911 2596 3564 3755 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 685 447 379 237 224 216
195 191 123 57
SfiI GGCCn_nnn'nGGCC
Cuts at: 406 406
Size: 4868
SfoI GGC'GCC
Cuts at: 198 2300 2548 198
Size: 2102 248 2518
Fragments arranged by size:
2518 2102 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4868
SmaI CCC'GGG
Cuts at: 1890 1890
Size: 4868
SmlI C'TyrA_G
Cuts at: 2023 2938 3652 3914 2023
Size: 915 714 262 2977
Fragments arranged by size:
2977 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4868
SphI G_CATG'C
Cuts at: 1921 2421 1921
Size: 500 4368
SspI AAT'ATT
Cuts at: 1432 1432
Size: 4868
StuI AGG'CCT
Cuts at: 400 973 991 1295 400
Size: 573 18 304 3973
Fragments arranged by size:
3973 573 304 18
StyI C'CwwG_G
Cuts at: 156 534 716 2002 2356 156
Size: 378 182 1286 354 2668
Fragments arranged by size:
2668 1286 378 354 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 120 334 511 2451 2584 3043 3252 120
Size: 214 177 1940 133 459 209 1736
Fragments arranged by size:
1940 1736 459 214 209 177 133
TatI w'GTAC_w
Cuts at: 1202 4383 1202
Size: 3181 1687
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 691 723 4385 4417 691
Size: 32 3662 32 1142
Fragments arranged by size:
3662 1142 32 32
Tth111I GACn'n_nGTC
Cuts at: 376 2665 376
Size: 2289 2579
XbaI T'CTAG_A
Cuts at: 1899 1899
Size: 4868
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 324 1041 2363 324
Size: 717 1322 2829
Fragments arranged by size:
2829 1322 717
XmaI C'CCGG_G
Cuts at: 1888 1888
Size: 4868
ZraI GAC'GTC
Cuts at: 1707 1707
Size: 4868
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AhdI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AleI AlfI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsiWI
BsmI BspEI BsrGI BssHII BstEII BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SgrAI SnaBI SrfI SwaI
XhoI XmnI