Restriction map for
pF1KB5059
(Circular) (Six-base) MAPSORT of: pF1KB5059.seq Check: 1347 from: 1 to: 4979
pF1KB5059 4979 bp
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1788 1788
Size: 4979
AccI GT'mk_AC
Cuts at: 2017 2017
Size: 4979
Acc65I G'GTAC_C
Cuts at: 1995 1995
Size: 4979
AclI AA'CG_TT
Cuts at: 4819 4819
Size: 4979
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 422 457 596 715 1212 2804 3236 3983 4251
Size: 35 139 119 497 1592 432 747 268
Cuts at: 4251 422
Size: 1150
Fragments arranged by size:
1592 1150 747 497 432 268 139 119
35
AfeI AGC'GCT
Cuts at: 1757 1757
Size: 4979
AflIII A'CryG_T
Cuts at: 2331 3435 4412 2331
Size: 1104 977 2898
Fragments arranged by size:
2898 1104 977
AgeI A'CCGG_T
Cuts at: 3367 3367
Size: 4979
AlwNI CAG_nnn'CTG
Cuts at: 243 255 1221 1803 2284 3851 243
Size: 12 966 582 481 1567 1371
Fragments arranged by size:
1567 1371 966 582 481 12
ApaI G_GGCC'C
Cuts at: 881 881
Size: 4979
ApaLI G'TGCA_C
Cuts at: 3749 3749
Size: 4979
ApoI r'AATT_y
Cuts at: 1983 4332 4588 1983
Size: 2349 256 2374
Fragments arranged by size:
2374 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4979
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4979
AvaI C'yCGr_G
Cuts at: 317 1068 1381 1683 1900 1999 317
Size: 751 313 302 217 99 3297
Fragments arranged by size:
3297 751 313 302 217 99
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1167 1200 1167
Size: 33 4946
BamHI G'GATC_C
Cuts at: 2004 2004
Size: 4979
BanI G'GyrC_C
Cuts at: 153 1073 1782 1995 2409 2657 2692 153
Size: 920 709 213 414 248 35 2440
Fragments arranged by size:
2440 920 709 414 248 213 35
BanII G_rGCy'C
Cuts at: 141 881 1993 3023 141
Size: 740 1112 1030 2097
Fragments arranged by size:
2097 1112 1030 740
BbeI G_GCGC'C
Cuts at: 1077 2413 2661 1077
Size: 1336 248 3395
Fragments arranged by size:
3395 1336 248
BbsI GAAGACnn'nnnn_
Cuts at: 273 1402 273
Size: 1129 3850
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 139 173 139
Size: 34 4945
BciVI GTATCCnnnnn_n'
Cuts at: 643 2877 3638 4979 643
Size: 2234 761 1341 643
Fragments arranged by size:
2234 1341 761 643
BclI T'GATC_A
Cuts at: 2499 2499
Size: 4979
BglI GCCn_nnn'nGGC
Cuts at: 225 1534 225
Size: 1309 3670
BglII A'GATC_T
Cuts at: 2494 4531 2494
Size: 2037 2942
BlpI GC'TnA_GC
Cuts at: 2091 2091
Size: 4979
Bme1580I G_kGCm'C
Cuts at: 212 881 1275 2604 2697 3753 212
Size: 669 394 1329 93 1056 1438
Fragments arranged by size:
1438 1329 1056 669 394 93
BmgBI CAC'GTC
Cuts at: 1440 1617 1440
Size: 177 4802
BmrI ACTGGGnnnn_n'
Cuts at: 2191 2272 2607 4751 2191
Size: 81 335 2144 2419
Fragments arranged by size:
2419 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 987 3346 987
Size: 2359 2620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 478 958 232
Size: 246 480 4253
Fragments arranged by size:
4253 480 246
Bpu10I CC'TnA_GC
Cuts at: 506 506
Size: 4979
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1453 2155 3034 3526 3824 1453
Size: 702 879 492 298 2608
Fragments arranged by size:
2608 879 702 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1024 4683 1024
Size: 3659 1320
BsaAI yAC'GTr
Cuts at: 207 518 2332 207
Size: 311 1814 2854
Fragments arranged by size:
2854 1814 311
BsaBI GATnn'nnATC
Cuts at: 6 775 2493 4530 6
Size: 769 1718 2037 455
Fragments arranged by size:
2037 1718 769 455
BsaHI Gr'CG_yC
Cuts at: 1074 1445 2410 2658 3360 4856 1074
Size: 371 965 248 702 1496 1197
Fragments arranged by size:
1496 1197 965 702 371 248
BsaWI w'CCGG_w
Cuts at: 1661 2168 2689 3367 3641 3788 4199 1661
Size: 507 521 678 274 147 411 2441
Fragments arranged by size:
2441 678 521 507 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1567 1597 1567
Size: 30 4949
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 1372 1654 1853 127
Size: 1245 282 199 3253
Fragments arranged by size:
3253 1245 282 199
BseYI C'CCAG_C
Cuts at: 1309 2404 3162 3739 4235 1309
Size: 1095 758 577 496 2053
Fragments arranged by size:
2053 1095 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1938 2521 2567 3775 104
Size: 1834 583 46 1208 1308
Fragments arranged by size:
1834 1308 1208 583 46
BsiHKAI G_wGCw'C
Cuts at: 1993 2771 2961 3753 1993
Size: 778 190 792 3219
Fragments arranged by size:
3219 792 778 190
BsmI GAATG_Cn'
Cuts at: 707 1537 707
Size: 830 4149
BsmBI CGTCTCn'nnnn_
Cuts at: 620 620
Size: 4979
Bsp1286I G_dGCh'C
Cuts at: 141 212 881 1275 1993 2604 2697 2771 2961
Size: 71 669 394 718 611 93 74 190
Cuts at: 2961 3023 3753 141
Size: 62 730 1367
Fragments arranged by size:
1367 730 718 669 611 394 190 93
74 71 62
BspEI T'CCGG_A
Cuts at: 4199 4199
Size: 4979
BspHI T'CATG_A
Cuts at: 4155 4155
Size: 4979
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1385 1469 1788 1911 2029 2243 2545 2926 1385
Size: 84 319 123 118 214 302 381 3438
Fragments arranged by size:
3438 381 319 302 214 123 118 84
BsrBI CCG'CTC
Cuts at: 50 173 3271 3325 4809 4974 50
Size: 123 3098 54 1484 165 55
Fragments arranged by size:
3098 1484 165 123 55 54
BsrDI GCAATG_nn'
Cuts at: 1806 2891 1806
Size: 1085 3894
BsrFI r'CCGG_y
Cuts at: 233 2977 3158 3367 233
Size: 2744 181 209 1845
Fragments arranged by size:
2744 1845 209 181
BsrGI T'GTAC_A
Cuts at: 1047 1656 1047
Size: 609 4370
BssHII G'CGCG_C
Cuts at: 406 406
Size: 4979
BssSI C'ACGA_G
Cuts at: 1453 3250 3608 1453
Size: 1797 358 2824
Fragments arranged by size:
2824 1797 358
BstAPI GCAn_nnn'nTGC
Cuts at: 243 1782 1803 2356 243
Size: 1539 21 553 2866
Fragments arranged by size:
2866 1539 553 21
BstBI TT'CG_AA
Cuts at: 3340 3340
Size: 4979
BstEII G'GTnAC_C
Cuts at: 854 854
Size: 4979
BstXI CCAn_nnnn'nTGG
Cuts at: 1299 1593 1299
Size: 294 4685
BstYI r'GATC_y
Cuts at: 1 452 731 2004 2471 2494 2829 3075 4087
Size: 451 279 1273 467 23 335 246 1012
Cuts at: 4087 4173 4185 4531 1
Size: 86 12 346 449
Fragments arranged by size:
1273 1012 467 451 449 346 335 279
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 927 1494 1964 107
Size: 820 567 470 3122
Fragments arranged by size:
3122 820 567 470
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 762 1683 2929 3110 3273 762
Size: 921 1246 181 163 2468
Fragments arranged by size:
2468 1246 921 181 163
BtsI GCAGTG_nn'
Cuts at: 2222 2222
Size: 4979
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1560 1595 1560
Size: 35 4944
DraI TTT'AAA
Cuts at: 1978 1978
Size: 4979
DrdI GACnn_nn'nnGTC
Cuts at: 2685 3543 2685
Size: 858 4121
EaeI y'GGCC_r
Cuts at: 759 1061 1668 2564 2738 3129 3156 4501 759
Size: 302 607 896 174 391 27 1345 1237
Fragments arranged by size:
1345 1237 896 607 391 302 174 27
EagI C'GGCC_G
Cuts at: 2564 2564
Size: 4979
EarI CTCTTCn'nnn_
Cuts at: 284 440 572 844 1231 3002 3212 284
Size: 156 132 272 387 1771 210 2051
Fragments arranged by size:
2051 1771 387 272 210 156 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 196 3187 3497 3643 4789 196
Size: 2991 310 146 1146 386
Fragments arranged by size:
2991 1146 386 310 146
EcoICRI GAG'CTC
Cuts at: 1991 1991
Size: 4979
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 422 457 478 596 715 958 1212 2804
Size: 190 35 21 118 119 243 254 1592
Cuts at: 2804 3236 3983 4251 232
Size: 432 747 268 960
Fragments arranged by size:
1592 960 747 432 268 254 243 190
119 118 35 21
EcoNI CCTnn'n_nnAGG
Cuts at: 161 619 958 1138 161
Size: 458 339 180 4002
Fragments arranged by size:
4002 458 339 180
EcoO109I rG'GnC_Cy
Cuts at: 346 877 878 1590 1866 2118 346
Size: 531 1 712 276 252 3207
Fragments arranged by size:
3207 712 531 276 252 1
EcoRI G'AATT_C
Cuts at: 1983 1983
Size: 4979
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2998 2998
Size: 4979
FspI TGC'GCA
Cuts at: 2760 2760
Size: 4979
HaeII r_GCGC'y
Cuts at: 234 1077 1683 1759 2413 2661 3683 4662 234
Size: 843 606 76 654 248 1022 979 551
Fragments arranged by size:
1022 979 843 654 606 551 248 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 617 649 878 910 1567 1599 2821 2851 2853
Size: 32 229 32 657 32 1222 30 2
Cuts at: 2853 2883 617
Size: 30 2713
Fragments arranged by size:
2713 1222 657 229 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2018 2018
Size: 4979
KasI G'GCGC_C
Cuts at: 1073 2409 2657 1073
Size: 1336 248 3395
Fragments arranged by size:
3395 1336 248
KpnI G_GTAC'C
Cuts at: 1999 1999
Size: 4979
MluI A'CGCG_T
Cuts at: 4412 4412
Size: 4979
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 98 380 1062 3650 3834 98
Size: 282 682 2588 184 1243
Fragments arranged by size:
2588 1243 682 282 184
MscI TGG'CCA
Cuts at: 1063 2740 1063
Size: 1677 3302
MslI CAynn'nnrTG
Cuts at: 616 1012 1057 1297 2968 3095 616
Size: 396 45 240 1671 127 2500
Fragments arranged by size:
2500 1671 396 240 127 45
MspA1I CmG'CkG
Cuts at: 305 435 542 2090 2404 2764 3777 4022 305
Size: 130 107 1548 314 360 1013 245 1262
Fragments arranged by size:
1548 1262 1013 360 314 245 130 107
NaeI GCC'GGC
Cuts at: 235 3160 235
Size: 2925 2054
NarI GG'CG_CC
Cuts at: 1074 2410 2658 1074
Size: 1336 248 3395
Fragments arranged by size:
3395 1336 248
NcoI C'CATG_G
Cuts at: 107 927 1494 1964 107
Size: 820 567 470 3122
Fragments arranged by size:
3122 820 567 470
NgoMIV G'CCGG_C
Cuts at: 233 3158 233
Size: 2925 2054
NspI r_CATG'y
Cuts at: 573 1011 1635 2032 2532 3439 573
Size: 438 624 397 500 907 2113
Fragments arranged by size:
2113 907 624 500 438 397
PciI A'CATG_T
Cuts at: 3435 3435
Size: 4979
PflMI CCAn_nnn'nTGG
Cuts at: 615 615
Size: 4979
PfoI T'CCnGG_A
Cuts at: 368 455 935 1334 1541 368
Size: 87 480 399 207 3806
Fragments arranged by size:
3806 480 399 207 87
PmeI GTTT'AAAC
Cuts at: 1978 1978
Size: 4979
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4118 4150 4118
Size: 32 4947
PshAI GACnn'nnGTC
Cuts at: 1118 1118
Size: 4979
PspOMI G'GGCC_C
Cuts at: 877 877
Size: 4979
PspXI vC'TCGA_Gb
Cuts at: 1381 1900 1381
Size: 519 4460
PstI C_TGCA'G
Cuts at: 263 678 962 1094 1394 1799 2026 2711 263
Size: 415 284 132 300 405 227 685 2531
Fragments arranged by size:
2531 685 415 405 300 284 227 132
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4979
PvuII CAG'CTG
Cuts at: 435 542 2404 2764 435
Size: 107 1862 360 2650
Fragments arranged by size:
2650 1862 360 107
RsrII CG'GwC_CG
Cuts at: 3174 3174
Size: 4979
SacI G_AGCT'C
Cuts at: 1993 1993
Size: 4979
SalI G'TCGA_C
Cuts at: 2016 2016
Size: 4979
SapI GCTCTTCn'nnn_
Cuts at: 284 572 1231 3002 3212 284
Size: 288 659 1771 210 2051
Fragments arranged by size:
2051 1771 659 288 210
SbfI CC_TGCA'GG
Cuts at: 2026 2026
Size: 4979
ScaI AGT'ACT
Cuts at: 943 4496 943
Size: 3553 1426
SfcI C'TryA_G
Cuts at: 33 259 674 958 1090 1390 1795 2022 2707
Size: 226 415 284 132 300 405 227 685
Cuts at: 2707 3700 3891 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 415 405 300 284 227
226 191 132
SfoI GGC'GCC
Cuts at: 1075 2411 2659 1075
Size: 1336 248 3395
Fragments arranged by size:
3395 1336 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4979
SmaI CCC'GGG
Cuts at: 319 1070 2001 319
Size: 751 931 3297
Fragments arranged by size:
3297 931 751
SmlI C'TyrA_G
Cuts at: 1381 1468 1900 2134 3049 3541 3803 1381
Size: 87 432 234 915 492 262 2557
Fragments arranged by size:
2557 915 492 432 262 234 87
SnaBI TAC'GTA
Cuts at: 518 518
Size: 4979
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4979
SphI G_CATG'C
Cuts at: 2032 2532 2032
Size: 500 4479
StuI AGG'CCT
Cuts at: 692 692
Size: 4979
StyI C'CwwG_G
Cuts at: 107 422 927 1494 1586 1964 2113 2467 107
Size: 315 505 567 92 378 149 354 2619
Fragments arranged by size:
2619 567 505 378 354 315 149 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 657 731 738 1924 2562 2695 3154 3363 657
Size: 74 7 1186 638 133 459 209 2273
Fragments arranged by size:
2273 1186 638 459 209 133 74 7
TatI w'GTAC_w
Cuts at: 941 1047 1410 1656 4494 941
Size: 106 363 246 2838 1426
Fragments arranged by size:
2838 1426 363 246 106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4496 4528 4496
Size: 32 4947
Tth111I GACn'n_nGTC
Cuts at: 2776 2776
Size: 4979
XbaI T'CTAG_A
Cuts at: 2010 2010
Size: 4979
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2474 2474
Size: 4979
XhoI C'TCGA_G
Cuts at: 1381 1900 1381
Size: 519 4460
XmaI C'CCGG_G
Cuts at: 317 1068 1999 317
Size: 751 931 3297
Fragments arranged by size:
3297 931 751
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PshAI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BbvCI BfrBI BmtI BsgI BsiWI BstZ17I Bsu36I ClaI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PsiI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
SspI SwaI XmnI ZraI