Restriction map for
pF1KB5050
(Circular) (Six-base) MAPSORT of: pF1KB5050.seq Check: 4662 from: 1 to: 4994
pF1KB5050 4994 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 1227 1467 2032 1227
Size: 240 565 4189
Fragments arranged by size:
4189 565 240
Acc65I G'GTAC_C
Cuts at: 2010 2010
Size: 4994
AclI AA'CG_TT
Cuts at: 4834 4834
Size: 4994
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 833 1964 2819 3251 3604 4266 328
Size: 505 1131 855 432 353 662 1056
Fragments arranged by size:
1131 1056 855 662 505 432 353
AflIII A'CryG_T
Cuts at: 787 1350 1472 1592 2346 4146 4427 787
Size: 563 122 120 754 1800 281 1354
Fragments arranged by size:
1800 1354 754 563 281 122 120
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1757 1757
Size: 4994
AlwNI CAG_nnn'CTG
Cuts at: 270 1216 1456 2299 3737 270
Size: 946 240 843 1438 1527
Fragments arranged by size:
1527 1438 946 843 240
ApaLI G'TGCA_C
Cuts at: 3832 3832
Size: 4994
ApoI r'AATT_y
Cuts at: 803 1831 1998 4347 4603 803
Size: 1028 167 2349 256 1194
Fragments arranged by size:
2349 1194 1028 256 167
AseI AT'TA_AT
Cuts at: 20 20
Size: 4994
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4994
AvaI C'yCGr_G
Cuts at: 117 1301 2014 117
Size: 1184 713 3097
Fragments arranged by size:
3097 1184 713
BamHI G'GATC_C
Cuts at: 1446 2019 1446
Size: 573 4421
BanI G'GyrC_C
Cuts at: 1604 2010 2424 2672 2707 1604
Size: 406 414 248 35 3891
Fragments arranged by size:
3891 414 406 248 35
BanII G_rGCy'C
Cuts at: 2008 3038 2008
Size: 1030 3964
BbeI G_GCGC'C
Cuts at: 2428 2676 2428
Size: 248 4746
BbsI GAAGACnn'nnnn_
Cuts at: 821 821
Size: 4994
BciVI GTATCCnnnnn_n'
Cuts at: 1531 2892 3948 4994 1531
Size: 1361 1056 1046 1531
Fragments arranged by size:
1531 1361 1056 1046
BclI T'GATC_A
Cuts at: 1008 1016 2514 1008
Size: 8 1498 3488
Fragments arranged by size:
3488 1498 8
BfrBI ATG'CAT
Cuts at: 1269 1269
Size: 4994
BglII A'GATC_T
Cuts at: 2509 4546 2509
Size: 2037 2957
BlpI GC'TnA_GC
Cuts at: 2106 2106
Size: 4994
Bme1580I G_kGCm'C
Cuts at: 298 1607 2619 2712 3836 298
Size: 1309 1012 93 1124 1456
Fragments arranged by size:
1456 1309 1124 1012 93
BmgBI CAC'GTC
Cuts at: 203 203
Size: 4994
BmrI ACTGGGnnnn_n'
Cuts at: 467 684 2206 2287 2622 4766 467
Size: 217 1522 81 335 2144 695
Fragments arranged by size:
2144 1522 695 335 217 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 420 991 1556 3361 420
Size: 571 565 1805 2053
Fragments arranged by size:
2053 1805 571 565
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 618 819 849 981 1238 553
Size: 65 201 30 132 257 4309
Fragments arranged by size:
4309 257 201 132 65 30
Bpu10I CC'TnA_GC
Cuts at: 208 208
Size: 4994
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2170 3049 3763 4061 2170
Size: 879 714 298 3103
Fragments arranged by size:
3103 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4698 4698
Size: 4994
BsaAI yAC'GTr
Cuts at: 2347 2347
Size: 4994
BsaBI GATnn'nnATC
Cuts at: 6 2508 4545 6
Size: 2502 2037 455
Fragments arranged by size:
2502 2037 455
BsaHI Gr'CG_yC
Cuts at: 534 2425 2673 3375 4871 534
Size: 1891 248 702 1496 657
Fragments arranged by size:
1891 1496 702 657 248
BsaWI w'CCGG_w
Cuts at: 2183 2704 3382 3793 3940 4214 2183
Size: 521 678 411 147 274 2963
Fragments arranged by size:
2963 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 983 983
Size: 4994
BseYI C'CCAG_C
Cuts at: 2419 3177 3842 4250 2419
Size: 758 665 408 3163
Fragments arranged by size:
3163 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1940 1940
Size: 4994
BsiEI CG_ry'CG
Cuts at: 104 2536 2582 3812 104
Size: 2432 46 1230 1286
Fragments arranged by size:
2432 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2008 2786 2976 3836 2008
Size: 778 190 860 3166
Fragments arranged by size:
3166 860 778 190
BsmI GAATG_Cn'
Cuts at: 1212 1586 1665 1212
Size: 374 79 4541
Fragments arranged by size:
4541 374 79
Bsp1286I G_dGCh'C
Cuts at: 298 1607 2008 2619 2712 2786 2976 3038 3836
Size: 1309 401 611 93 74 190 62 798
Cuts at: 3836 298
Size: 1456
Fragments arranged by size:
1456 1309 798 611 401 190 93 74
62
BspHI T'CATG_A
Cuts at: 1275 3426 1275
Size: 2151 2843
BspMI ACCTGCnnnn'nnnn_
Cuts at: 776 2044 2258 2560 2941 776
Size: 1268 214 302 381 2829
Fragments arranged by size:
2829 1268 381 302 214
BsrBI CCG'CTC
Cuts at: 50 404 3286 3340 4824 4989 50
Size: 354 2882 54 1484 165 55
Fragments arranged by size:
2882 1484 354 165 55 54
BsrDI GCAATG_nn'
Cuts at: 712 865 1714 2906 712
Size: 153 849 1192 2800
Fragments arranged by size:
2800 1192 849 153
BsrFI r'CCGG_y
Cuts at: 171 2992 3173 171
Size: 2821 181 1992
Fragments arranged by size:
2821 1992 181
BssSI C'ACGA_G
Cuts at: 3265 3973 3265
Size: 708 4286
BstAPI GCAn_nnn'nTGC
Cuts at: 2371 2371
Size: 4994
BstBI TT'CG_AA
Cuts at: 1839 3355 1839
Size: 1516 3478
BstEII G'GTnAC_C
Cuts at: 1039 1039
Size: 4994
BstXI CCAn_nnnn'nTGG
Cuts at: 1959 1959
Size: 4994
BstYI r'GATC_y
Cuts at: 1 1177 1446 1537 2019 2486 2509 2844 3090
Size: 1176 269 91 482 467 23 335 246
Cuts at: 3090 3396 3408 3494 4546 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1176 1052 482 467 449 335 306 269
246 91 86 23 12
BstZ17I GTA'TAC
Cuts at: 1228 1228
Size: 4994
Bsu36I CC'TnA_GG
Cuts at: 986 986
Size: 4994
BtgI C'CryG_G
Cuts at: 387 916 387
Size: 529 4465
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2944 3125 3288 2944
Size: 181 163 4650
Fragments arranged by size:
4650 181 163
BtsI GCAGTG_nn'
Cuts at: 2237 2237
Size: 4994
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 444 479 444
Size: 35 4959
DraI TTT'AAA
Cuts at: 1076 1993 1076
Size: 917 4077
DrdI GACnn_nn'nnGTC
Cuts at: 2700 4044 2700
Size: 1344 3650
EaeI y'GGCC_r
Cuts at: 516 2579 2753 3144 3171 4516 516
Size: 2063 174 391 27 1345 994
Fragments arranged by size:
2063 1345 994 391 174 27
EagI C'GGCC_G
Cuts at: 2579 2579
Size: 4994
EarI CTCTTCn'nnn_
Cuts at: 842 3017 3227 842
Size: 2175 210 2609
Fragments arranged by size:
2609 2175 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1902 3202 3944 4090 4804 1902
Size: 1300 742 146 714 2092
Fragments arranged by size:
2092 1300 742 714 146
EcoICRI GAG'CTC
Cuts at: 2006 2006
Size: 4994
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 553 618 819 833 849 981 1238 1964
Size: 225 65 201 14 16 132 257 726
Cuts at: 1964 2819 3251 3604 4266 328
Size: 855 432 353 662 1056
Fragments arranged by size:
1056 855 726 662 432 353 257 225
201 132 65 16 14
EcoNI CCTnn'n_nnAGG
Cuts at: 248 248
Size: 4994
EcoO109I rG'GnC_Cy
Cuts at: 498 1723 2133 498
Size: 1225 410 3359
Fragments arranged by size:
3359 1225 410
EcoRI G'AATT_C
Cuts at: 1998 1998
Size: 4994
EcoRV GAT'ATC
Cuts at: 1689 1689
Size: 4994
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 838 3013 838
Size: 2175 2819
FspI TGC'GCA
Cuts at: 2775 2775
Size: 4994
HaeII r_GCGC'y
Cuts at: 2428 2676 3906 4677 2428
Size: 248 1230 771 2745
Fragments arranged by size:
2745 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1148 1180 2836 2866 2868 2898 1148
Size: 32 1656 30 2 30 3244
Fragments arranged by size:
3244 1656 32 30 30 2
HincII GTy'rAC
Cuts at: 1598 1616 2033 1598
Size: 18 417 4559
Fragments arranged by size:
4559 417 18
HpaI GTT'AAC
Cuts at: 1598 1616 1598
Size: 18 4976
KasI G'GCGC_C
Cuts at: 2424 2672 2424
Size: 248 4746
KpnI G_GTAC'C
Cuts at: 2014 2014
Size: 4994
MluI A'CGCG_T
Cuts at: 4427 4427
Size: 4994
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 362 1912 3753 3937 362
Size: 1550 1841 184 1419
Fragments arranged by size:
1841 1550 1419 184
MscI TGG'CCA
Cuts at: 518 2755 518
Size: 2237 2757
MslI CAynn'nnrTG
Cuts at: 1130 1274 1739 2983 3110 1130
Size: 144 465 1244 127 3014
Fragments arranged by size:
3014 1244 465 144 127
MspA1I CmG'CkG
Cuts at: 1382 2105 2419 2779 3563 3808 1382
Size: 723 314 360 784 245 2568
Fragments arranged by size:
2568 784 723 360 314 245
NaeI GCC'GGC
Cuts at: 3175 3175
Size: 4994
NarI GG'CG_CC
Cuts at: 2425 2673 2425
Size: 248 4746
NcoI C'CATG_G
Cuts at: 387 916 387
Size: 529 4465
NdeI CA'TA_TG
Cuts at: 668 1422 668
Size: 754 4240
NgoMIV G'CCGG_C
Cuts at: 3173 3173
Size: 4994
NsiI A_TGCA'T
Cuts at: 1271 1271
Size: 4994
NspI r_CATG'y
Cuts at: 216 791 1025 1354 2047 2547 4150 216
Size: 575 234 329 693 500 1603 1060
Fragments arranged by size:
1603 1060 693 575 500 329 234
PciI A'CATG_T
Cuts at: 787 1350 4146 787
Size: 563 2796 1635
Fragments arranged by size:
2796 1635 563
PfoI T'CCnGG_A
Cuts at: 569 752 569
Size: 183 4811
PmeI GTTT'AAAC
Cuts at: 1993 1993
Size: 4994
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3440 3472 3440
Size: 32 4962
PpuMI rG'GwC_Cy
Cuts at: 498 1723 498
Size: 1225 3769
PstI C_TGCA'G
Cuts at: 2041 2726 2041
Size: 685 4309
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4994
PvuII CAG'CTG
Cuts at: 2419 2779 2419
Size: 360 4634
RsrII CG'GwC_CG
Cuts at: 3189 3189
Size: 4994
SacI G_AGCT'C
Cuts at: 2008 2008
Size: 4994
SalI G'TCGA_C
Cuts at: 2031 2031
Size: 4994
SapI GCTCTTCn'nnn_
Cuts at: 3017 3227 3017
Size: 210 4784
SbfI CC_TGCA'GG
Cuts at: 2041 2041
Size: 4994
ScaI AGT'ACT
Cuts at: 4511 4511
Size: 4994
SexAI A'CCwGG_T
Cuts at: 897 897
Size: 4994
SfcI C'TryA_G
Cuts at: 33 560 1181 1670 2037 2722 3690 3881 33
Size: 527 621 489 367 685 968 191 1146
Fragments arranged by size:
1146 968 685 621 527 489 367 191
SfoI GGC'GCC
Cuts at: 2426 2674 2426
Size: 248 4746
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4994
SmaI CCC'GGG
Cuts at: 2016 2016
Size: 4994
SmlI C'TyrA_G
Cuts at: 1301 2149 3064 3778 4040 1301
Size: 848 915 714 262 2255
Fragments arranged by size:
2255 915 848 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4994
SphI G_CATG'C
Cuts at: 2047 2547 2047
Size: 500 4494
SspI AAT'ATT
Cuts at: 1644 1644
Size: 4994
StuI AGG'CCT
Cuts at: 566 566
Size: 4994
StyI C'CwwG_G
Cuts at: 387 519 916 2128 2482 387
Size: 132 397 1212 354 2899
Fragments arranged by size:
2899 1212 397 354 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2577 2710 3169 3378 2577
Size: 133 459 209 4193
Fragments arranged by size:
4193 459 209 133
TatI w'GTAC_w
Cuts at: 4509 4509
Size: 4994
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4511 4543 4511
Size: 32 4962
Tth111I GACn'n_nGTC
Cuts at: 2791 2791
Size: 4994
XbaI T'CTAG_A
Cuts at: 2025 2025
Size: 4994
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1531 2489 1531
Size: 958 4036
XhoI C'TCGA_G
Cuts at: 1301 1301
Size: 4994
XmaI C'CCGG_G
Cuts at: 2014 2014
Size: 4994
XmnI GAAnn'nnTTC
Cuts at: 1835 1835
Size: 4994
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PciI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmtI
BsaXI BsiWI BsmBI BspEI BsrGI BssHII ClaI DraIII
FseI FspAI HindIII MfeI NheI NotI NruI PacI
PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SwaI ZraI