Restriction map for
pF1KB5046
(Circular) (Six-base) MAPSORT of: pF1KB5046.seq Check: 7304 from: 1 to: 4301
pF1KB5046 4301 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1339 1339
Size: 4301
Acc65I G'GTAC_C
Cuts at: 1317 1317
Size: 4301
AclI AA'CG_TT
Cuts at: 4141 4141
Size: 4301
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 631 2126 2558 2911 3573 292
Size: 339 1495 432 353 662 1020
Fragments arranged by size:
1495 1020 662 432 353 339
AflIII A'CryG_T
Cuts at: 1653 3453 3734 1653
Size: 1800 281 2220
Fragments arranged by size:
2220 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 315 734 804 1185 1606 3044 315
Size: 419 70 381 421 1438 1572
Fragments arranged by size:
1572 1438 421 419 381 70
ApaI G_GGCC'C
Cuts at: 483 843 1080 483
Size: 360 237 3704
Fragments arranged by size:
3704 360 237
ApaLI G'TGCA_C
Cuts at: 3139 3139
Size: 4301
ApoI r'AATT_y
Cuts at: 1305 3654 3910 1305
Size: 2349 256 1696
Fragments arranged by size:
2349 1696 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4301
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4301
AvaI C'yCGr_G
Cuts at: 523 1321 523
Size: 798 3503
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 261 294 261
Size: 33 4268
BamHI G'GATC_C
Cuts at: 1326 1326
Size: 4301
BanI G'GyrC_C
Cuts at: 1225 1317 1731 1979 2014 1225
Size: 92 414 248 35 3512
Fragments arranged by size:
3512 414 248 92 35
BanII G_rGCy'C
Cuts at: 483 541 747 843 998 1080 1315 2345 483
Size: 58 206 96 155 82 235 1030 2439
Fragments arranged by size:
2439 1030 235 206 155 96 82 58
BbeI G_GCGC'C
Cuts at: 1735 1983 1735
Size: 248 4053
BciVI GTATCCnnnnn_n'
Cuts at: 210 2199 3255 4301 210
Size: 1989 1056 1046 210
Fragments arranged by size:
1989 1056 1046 210
BclI T'GATC_A
Cuts at: 1821 1821
Size: 4301
BglI GCCn_nnn'nGGC
Cuts at: 847 847
Size: 4301
BglII A'GATC_T
Cuts at: 1816 3853 1816
Size: 2037 2264
BlpI GC'TnA_GC
Cuts at: 1413 1413
Size: 4301
Bme1580I G_kGCm'C
Cuts at: 483 843 1080 1230 1926 2019 3143 483
Size: 360 237 150 696 93 1124 1641
Fragments arranged by size:
1641 1124 696 360 237 150 93
BmrI ACTGGGnnnn_n'
Cuts at: 1513 1594 1929 4073 1513
Size: 81 335 2144 1741
Fragments arranged by size:
2144 1741 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 763 2668 763
Size: 1905 2396
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 525 781 946 1171 352
Size: 173 256 165 225 3482
Fragments arranged by size:
3482 256 225 173 165
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 231 856 1123 1477 2356 3070 3368 231
Size: 625 267 354 879 714 298 1164
Fragments arranged by size:
1164 879 714 625 354 298 267
BsaI GGTCTCn'nnnn_
Cuts at: 4005 4005
Size: 4301
BsaAI yAC'GTr
Cuts at: 1654 1654
Size: 4301
BsaBI GATnn'nnATC
Cuts at: 6 1815 3852 6
Size: 1809 2037 455
Fragments arranged by size:
2037 1809 455
BsaHI Gr'CG_yC
Cuts at: 854 1235 1732 1980 2682 4178 854
Size: 381 497 248 702 1496 977
Fragments arranged by size:
1496 977 702 497 381 248
BsaWI w'CCGG_w
Cuts at: 709 1490 2011 2689 3100 3247 3521 709
Size: 781 521 678 411 147 274 1489
Fragments arranged by size:
1489 781 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 607 755 1126 607
Size: 148 371 3782
Fragments arranged by size:
3782 371 148
BseYI C'CCAG_C
Cuts at: 942 963 1726 2484 3149 3557 942
Size: 21 763 758 665 408 1686
Fragments arranged by size:
1686 763 758 665 408 21
BsiEI CG_ry'CG
Cuts at: 104 1843 1889 3119 104
Size: 1739 46 1230 1286
Fragments arranged by size:
1739 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 998 1315 2093 2283 3143 998
Size: 317 778 190 860 2156
Fragments arranged by size:
2156 860 778 317 190
BsmI GAATG_Cn'
Cuts at: 1029 1029
Size: 4301
BsmBI CGTCTCn'nnnn_
Cuts at: 1242 1242
Size: 4301
Bsp1286I G_dGCh'C
Cuts at: 483 541 747 843 998 1080 1230 1315 1926
Size: 58 206 96 155 82 150 85 611
Cuts at: 1926 2019 2093 2283 2345 3143 483
Size: 93 74 190 62 798 1641
Fragments arranged by size:
1641 798 611 206 190 155 150 96
93 85 82 74 62 58
BspEI T'CCGG_A
Cuts at: 709 709
Size: 4301
BspHI T'CATG_A
Cuts at: 2733 2733
Size: 4301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1351 1565 1867 2248 1351
Size: 214 302 381 3404
Fragments arranged by size:
3404 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2593 2647 4131 4296 50
Size: 2543 54 1484 165 55
Fragments arranged by size:
2543 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 249 2213 249
Size: 1964 2337
BsrFI r'CCGG_y
Cuts at: 2299 2480 2299
Size: 181 4120
BssSI C'ACGA_G
Cuts at: 982 2572 3280 982
Size: 1590 708 2003
Fragments arranged by size:
2003 1590 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1678 1678
Size: 4301
BstBI TT'CG_AA
Cuts at: 2662 2662
Size: 4301
BstEII G'GTnAC_C
Cuts at: 1068 1068
Size: 4301
BstXI CCAn_nnnn'nTGG
Cuts at: 244 924 1260 244
Size: 680 336 3285
Fragments arranged by size:
3285 680 336
BstYI r'GATC_y
Cuts at: 1 596 800 1326 1793 1816 2151 2397 2703
Size: 595 204 526 467 23 335 246 306
Cuts at: 2703 2715 2801 3853 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 595 526 467 449 335 306 246
204 86 23 12
BtgI C'CryG_G
Cuts at: 170 1080 170
Size: 910 3391
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2251 2432 2595 2251
Size: 181 163 3957
Fragments arranged by size:
3957 181 163
BtsI GCAGTG_nn'
Cuts at: 1544 1544
Size: 4301
DraI TTT'AAA
Cuts at: 1300 1300
Size: 4301
DrdI GACnn_nn'nnGTC
Cuts at: 412 724 1270 2007 3351 412
Size: 312 546 737 1344 1362
Fragments arranged by size:
1362 1344 737 546 312
EaeI y'GGCC_r
Cuts at: 299 1886 2060 2451 2478 3823 299
Size: 1587 174 391 27 1345 777
Fragments arranged by size:
1587 1345 777 391 174 27
EagI C'GGCC_G
Cuts at: 1886 1886
Size: 4301
EarI CTCTTCn'nnn_
Cuts at: 986 2324 2534 986
Size: 1338 210 2753
Fragments arranged by size:
2753 1338 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2509 3251 3397 4111 2509
Size: 742 146 714 2699
Fragments arranged by size:
2699 742 714 146
EcoICRI GAG'CTC
Cuts at: 996 1313 996
Size: 317 3984
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 352 525 631 781 946 1171 2126 2558
Size: 60 173 106 150 165 225 955 432
Cuts at: 2558 2911 3573 292
Size: 353 662 1020
Fragments arranged by size:
1020 955 662 432 353 225 173 165
150 106 60
EcoNI CCTnn'n_nnAGG
Cuts at: 780 780
Size: 4301
EcoO109I rG'GnC_Cy
Cuts at: 479 480 839 840 1076 1182 1440 479
Size: 1 359 1 236 106 258 3340
Fragments arranged by size:
3340 359 258 236 106 1 1
EcoRI G'AATT_C
Cuts at: 1305 1305
Size: 4301
EcoRV GAT'ATC
Cuts at: 498 498
Size: 4301
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 169 690 2320 169
Size: 521 1630 2150
Fragments arranged by size:
2150 1630 521
FspI TGC'GCA
Cuts at: 2082 2082
Size: 4301
HaeII r_GCGC'y
Cuts at: 426 548 1735 1983 3213 3984 426
Size: 122 1187 248 1230 771 743
Fragments arranged by size:
1230 1187 771 743 248 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 904 936 2143 2173 2175 2205 904
Size: 32 1207 30 2 30 3000
Fragments arranged by size:
3000 1207 32 30 30 2
HincII GTy'rAC
Cuts at: 630 1340 630
Size: 710 3591
HindIII A'AGCT_T
Cuts at: 324 324
Size: 4301
KasI G'GCGC_C
Cuts at: 1731 1979 1731
Size: 248 4053
KpnI G_GTAC'C
Cuts at: 1321 1321
Size: 4301
MluI A'CGCG_T
Cuts at: 3734 3734
Size: 4301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 491 3060 3244 491
Size: 2569 184 1548
Fragments arranged by size:
2569 1548 184
MscI TGG'CCA
Cuts at: 301 2062 301
Size: 1761 2540
MslI CAynn'nnrTG
Cuts at: 242 1003 1022 2290 2417 242
Size: 761 19 1268 127 2126
Fragments arranged by size:
2126 1268 761 127 19
MspA1I CmG'CkG
Cuts at: 609 1082 1193 1412 1726 2086 2870 3115 609
Size: 473 111 219 314 360 784 245 1795
Fragments arranged by size:
1795 784 473 360 314 245 219 111
NaeI GCC'GGC
Cuts at: 2482 2482
Size: 4301
NarI GG'CG_CC
Cuts at: 1732 1980 1732
Size: 248 4053
NgoMIV G'CCGG_C
Cuts at: 2480 2480
Size: 4301
NspI r_CATG'y
Cuts at: 169 1354 1854 3457 169
Size: 1185 500 1603 1013
Fragments arranged by size:
1603 1185 1013 500
PciI A'CATG_T
Cuts at: 3453 3453
Size: 4301
PmeI GTTT'AAAC
Cuts at: 1300 1300
Size: 4301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2747 2779 2747
Size: 32 4269
PspOMI G'GGCC_C
Cuts at: 479 839 1076 479
Size: 360 237 3704
Fragments arranged by size:
3704 360 237
PstI C_TGCA'G
Cuts at: 1348 2033 1348
Size: 685 3616
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4301
PvuII CAG'CTG
Cuts at: 1193 1726 2086 1193
Size: 533 360 3408
Fragments arranged by size:
3408 533 360
RsrII CG'GwC_CG
Cuts at: 2496 2496
Size: 4301
SacI G_AGCT'C
Cuts at: 998 1315 998
Size: 317 3984
SacII CC_GC'GG
Cuts at: 1083 1083
Size: 4301
SalI G'TCGA_C
Cuts at: 1338 1338
Size: 4301
SapI GCTCTTCn'nnn_
Cuts at: 986 2324 2534 986
Size: 1338 210 2753
Fragments arranged by size:
2753 1338 210
SbfI CC_TGCA'GG
Cuts at: 1348 1348
Size: 4301
ScaI AGT'ACT
Cuts at: 3818 3818
Size: 4301
SfcI C'TryA_G
Cuts at: 33 1344 2029 2997 3188 33
Size: 1311 685 968 191 1146
Fragments arranged by size:
1311 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1733 1981 1733
Size: 248 4053
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4301
SmaI CCC'GGG
Cuts at: 525 1323 525
Size: 798 3503
SmlI C'TyrA_G
Cuts at: 210 871 1138 1456 2371 3085 3347 210
Size: 661 267 318 915 714 262 1164
Fragments arranged by size:
1164 915 714 661 318 267 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4301
SphI G_CATG'C
Cuts at: 1354 1854 1354
Size: 500 3801
StyI C'CwwG_G
Cuts at: 641 678 1435 1789 641
Size: 37 757 354 3153
Fragments arranged by size:
3153 757 354 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 369 1884 2017 2476 2685 369
Size: 1515 133 459 209 1985
Fragments arranged by size:
1985 1515 459 209 133
TatI w'GTAC_w
Cuts at: 3816 3816
Size: 4301
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3818 3850 3818
Size: 32 4269
Tth111I GACn'n_nGTC
Cuts at: 2098 2098
Size: 4301
XbaI T'CTAG_A
Cuts at: 1332 1332
Size: 4301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 648 843 1796 648
Size: 195 953 3153
Fragments arranged by size:
3153 953 195
XmaI C'CCGG_G
Cuts at: 523 1321 523
Size: 798 3503
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI AscI AvrII BbsI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsrGI BssHII BstZ17I
Bsu36I ClaI CspCI DraIII FseI FspAI HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI