Restriction map for
pF1KB5025
(Circular) (Six-base) MAPSORT of: pF1KB5025.seq Check: 5676 from: 1 to: 3830
pF1KB5025 3830 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 868 868
Size: 3830
Acc65I G'GTAC_C
Cuts at: 846 846
Size: 3830
AclI AA'CG_TT
Cuts at: 3670 3670
Size: 3830
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 520 1655 2087 2440 3102 472
Size: 48 1135 432 353 662 1200
Fragments arranged by size:
1200 1135 662 432 353 48
AflIII A'CryG_T
Cuts at: 1182 2982 3263 1182
Size: 1800 281 1749
Fragments arranged by size:
1800 1749 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 385 417 385
Size: 32 3798
AlwNI CAG_nnn'CTG
Cuts at: 1135 2573 1135
Size: 1438 2392
ApaI G_GGCC'C
Cuts at: 480 480
Size: 3830
ApaLI G'TGCA_C
Cuts at: 2668 2668
Size: 3830
ApoI r'AATT_y
Cuts at: 834 3183 3439 834
Size: 2349 256 1225
Fragments arranged by size:
2349 1225 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3830
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3830
AvaI C'yCGr_G
Cuts at: 220 226 850 220
Size: 6 624 3200
Fragments arranged by size:
3200 624 6
BamHI G'GATC_C
Cuts at: 855 855
Size: 3830
BanI G'GyrC_C
Cuts at: 196 667 846 1260 1508 1543 196
Size: 471 179 414 248 35 2483
Fragments arranged by size:
2483 471 414 248 179 35
BanII G_rGCy'C
Cuts at: 215 227 480 844 1874 215
Size: 12 253 364 1030 2171
Fragments arranged by size:
2171 1030 364 253 12
BbeI G_GCGC'C
Cuts at: 200 1264 1512 200
Size: 1064 248 2518
Fragments arranged by size:
2518 1064 248
BbsI GAAGACnn'nnnn_
Cuts at: 330 330
Size: 3830
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 295 329 295
Size: 34 3796
BciVI GTATCCnnnnn_n'
Cuts at: 1728 2784 3830 1728
Size: 1056 1046 1728
Fragments arranged by size:
1728 1056 1046
BclI T'GATC_A
Cuts at: 509 1350 509
Size: 841 2989
BglII A'GATC_T
Cuts at: 1345 3382 1345
Size: 2037 1793
BlpI GC'TnA_GC
Cuts at: 271 283 544 740 942 271
Size: 12 261 196 202 3159
Fragments arranged by size:
3159 261 202 196 12
Bme1580I G_kGCm'C
Cuts at: 480 1455 1548 2672 480
Size: 975 93 1124 1638
Fragments arranged by size:
1638 1124 975 93
BmrI ACTGGGnnnn_n'
Cuts at: 1042 1123 1458 3602 1042
Size: 81 335 2144 1270
Fragments arranged by size:
2144 1270 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 3830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 412 673 157
Size: 255 261 3314
Fragments arranged by size:
3314 261 255
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 790 1006 1885 2599 2897 790
Size: 216 879 714 298 1723
Fragments arranged by size:
1723 879 714 298 216
BsaI GGTCTCn'nnnn_
Cuts at: 3534 3534
Size: 3830
BsaAI yAC'GTr
Cuts at: 1183 1183
Size: 3830
BsaBI GATnn'nnATC
Cuts at: 6 1344 3381 6
Size: 1338 2037 455
Fragments arranged by size:
2037 1338 455
BsaHI Gr'CG_yC
Cuts at: 197 1261 1509 2211 3707 197
Size: 1064 248 702 1496 320
Fragments arranged by size:
1496 1064 702 320 248
BsaWI w'CCGG_w
Cuts at: 1019 1540 2218 2629 2776 3050 1019
Size: 521 678 411 147 274 1799
Fragments arranged by size:
1799 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 112 142 385 415 112
Size: 30 243 30 3527
Fragments arranged by size:
3527 243 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 133 172 181 778 781 784 133
Size: 39 9 597 3 3 3179
Fragments arranged by size:
3179 597 39 9 3 3
BseYI C'CCAG_C
Cuts at: 179 201 278 418 471 1255 2013 2678 3086
Size: 22 77 140 53 784 758 665 408
Cuts at: 3086 179
Size: 923
Fragments arranged by size:
923 784 758 665 408 140 77 53
22
BsiEI CG_ry'CG
Cuts at: 104 1372 1418 2648 104
Size: 1268 46 1230 1286
Fragments arranged by size:
1286 1268 1230 46
BsiHKAI G_wGCw'C
Cuts at: 844 1622 1812 2672 844
Size: 778 190 860 2002
Fragments arranged by size:
2002 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 215 227 480 844 1455 1548 1622 1812 1874
Size: 12 253 364 611 93 74 190 62
Cuts at: 1874 2672 215
Size: 798 1373
Fragments arranged by size:
1373 798 611 364 253 190 93 74
62 12
BspHI T'CATG_A
Cuts at: 2262 2262
Size: 3830
BspMI ACCTGCnnnn'nnnn_
Cuts at: 880 1094 1396 1777 880
Size: 214 302 381 2933
Fragments arranged by size:
2933 381 302 214
BsrBI CCG'CTC
Cuts at: 50 753 2122 2176 3660 3825 50
Size: 703 1369 54 1484 165 55
Fragments arranged by size:
1484 1369 703 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1742 1742
Size: 3830
BsrFI r'CCGG_y
Cuts at: 143 287 1828 2009 143
Size: 144 1541 181 1964
Fragments arranged by size:
1964 1541 181 144
BssSI C'ACGA_G
Cuts at: 2101 2809 2101
Size: 708 3122
BstAPI GCAn_nnn'nTGC
Cuts at: 1207 1207
Size: 3830
BstBI TT'CG_AA
Cuts at: 2191 2191
Size: 3830
BstYI r'GATC_y
Cuts at: 1 855 1322 1345 1680 1926 2232 2244 2330
Size: 854 467 23 335 246 306 12 86
Cuts at: 2330 3382 1
Size: 1052 449
Fragments arranged by size:
1052 854 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 248 581 107
Size: 141 333 3356
Fragments arranged by size:
3356 333 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1780 1961 2124 1780
Size: 181 163 3486
Fragments arranged by size:
3486 181 163
BtsI GCAGTG_nn'
Cuts at: 1073 1073
Size: 3830
ClaI AT'CG_AT
Cuts at: 641 641
Size: 3830
DraI TTT'AAA
Cuts at: 829 829
Size: 3830
DrdI GACnn_nn'nnGTC
Cuts at: 1536 2880 1536
Size: 1344 2486
EaeI y'GGCC_r
Cuts at: 110 251 710 1415 1589 1980 2007 3352 110
Size: 141 459 705 174 391 27 1345 588
Fragments arranged by size:
1345 705 588 459 391 174 141 27
EagI C'GGCC_G
Cuts at: 1415 1415
Size: 3830
EarI CTCTTCn'nnn_
Cuts at: 496 1853 2063 496
Size: 1357 210 2263
Fragments arranged by size:
2263 1357 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 772 2038 2780 2926 3640 772
Size: 1266 742 146 714 962
Fragments arranged by size:
1266 962 742 714 146
EcoICRI GAG'CTC
Cuts at: 842 842
Size: 3830
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 412 472 520 673 1655 2087 2440 3102
Size: 255 60 48 153 982 432 353 662
Cuts at: 3102 157
Size: 885
Fragments arranged by size:
982 885 662 432 353 255 153 60
48
EcoNI CCTnn'n_nnAGG
Cuts at: 382 382
Size: 3830
EcoO109I rG'GnC_Cy
Cuts at: 387 476 477 969 387
Size: 89 1 492 3248
Fragments arranged by size:
3248 492 89 1
EcoRI G'AATT_C
Cuts at: 834 834
Size: 3830
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 564 696 831 1849 564
Size: 132 135 1018 2545
Fragments arranged by size:
2545 1018 135 132
FspI TGC'GCA
Cuts at: 1611 1611
Size: 3830
HaeII r_GCGC'y
Cuts at: 144 200 260 278 314 330 622 1264 1512
Size: 56 60 18 36 16 292 642 248
Cuts at: 1512 2742 3513 144
Size: 1230 771 461
Fragments arranged by size:
1230 771 642 461 292 248 60 56
36 18 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1672 1702 1704 1734 1672
Size: 30 2 30 3768
Fragments arranged by size:
3768 30 30 2
HincII GTy'rAC
Cuts at: 869 869
Size: 3830
HindIII A'AGCT_T
Cuts at: 628 628
Size: 3830
KasI G'GCGC_C
Cuts at: 196 1260 1508 196
Size: 1064 248 2518
Fragments arranged by size:
2518 1064 248
KpnI G_GTAC'C
Cuts at: 850 850
Size: 3830
MluI A'CGCG_T
Cuts at: 3263 3263
Size: 3830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2589 2773 2589
Size: 184 3646
MscI TGG'CCA
Cuts at: 112 1591 112
Size: 1479 2351
MslI CAynn'nnrTG
Cuts at: 1819 1946 1819
Size: 127 3703
MspA1I CmG'CkG
Cuts at: 183 282 583 941 1255 1615 2399 2644 183
Size: 99 301 358 314 360 784 245 1369
Fragments arranged by size:
1369 784 360 358 314 301 245 99
NaeI GCC'GGC
Cuts at: 145 289 2011 145
Size: 144 1722 1964
Fragments arranged by size:
1964 1722 144
NarI GG'CG_CC
Cuts at: 197 1261 1509 197
Size: 1064 248 2518
Fragments arranged by size:
2518 1064 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3830
NgoMIV G'CCGG_C
Cuts at: 143 287 2009 143
Size: 144 1722 1964
Fragments arranged by size:
1964 1722 144
NspI r_CATG'y
Cuts at: 402 883 1383 2986 402
Size: 481 500 1603 1246
Fragments arranged by size:
1603 1246 500 481
PciI A'CATG_T
Cuts at: 2982 2982
Size: 3830
PfoI T'CCnGG_A
Cuts at: 359 551 359
Size: 192 3638
PmeI GTTT'AAAC
Cuts at: 829 829
Size: 3830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 385 417 2276 2308 385
Size: 32 1859 32 1907
Fragments arranged by size:
1907 1859 32 32
PpuMI rG'GwC_Cy
Cuts at: 387 387
Size: 3830
PspOMI G'GGCC_C
Cuts at: 476 476
Size: 3830
PstI C_TGCA'G
Cuts at: 334 599 877 1562 334
Size: 265 278 685 2602
Fragments arranged by size:
2602 685 278 265
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3830
PvuII CAG'CTG
Cuts at: 183 282 1255 1615 183
Size: 99 973 360 2398
Fragments arranged by size:
2398 973 360 99
RsrII CG'GwC_CG
Cuts at: 2025 2025
Size: 3830
SacI G_AGCT'C
Cuts at: 844 844
Size: 3830
SacII CC_GC'GG
Cuts at: 584 584
Size: 3830
SalI G'TCGA_C
Cuts at: 867 867
Size: 3830
SapI GCTCTTCn'nnn_
Cuts at: 496 1853 2063 496
Size: 1357 210 2263
Fragments arranged by size:
2263 1357 210
SbfI CC_TGCA'GG
Cuts at: 877 877
Size: 3830
ScaI AGT'ACT
Cuts at: 3347 3347
Size: 3830
SfcI C'TryA_G
Cuts at: 33 330 595 873 1558 2526 2717 33
Size: 297 265 278 685 968 191 1146
Fragments arranged by size:
1146 968 685 297 278 265 191
SfoI GGC'GCC
Cuts at: 198 1262 1510 198
Size: 1064 248 2518
Fragments arranged by size:
2518 1064 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3830
SmaI CCC'GGG
Cuts at: 852 852
Size: 3830
SmlI C'TyrA_G
Cuts at: 805 985 1900 2614 2876 805
Size: 180 915 714 262 1759
Fragments arranged by size:
1759 915 714 262 180
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3830
SphI G_CATG'C
Cuts at: 883 1383 883
Size: 500 3330
StyI C'CwwG_G
Cuts at: 107 689 964 1318 107
Size: 582 275 354 2619
Fragments arranged by size:
2619 582 354 275
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1413 1546 2005 2214 1413
Size: 133 459 209 3029
Fragments arranged by size:
3029 459 209 133
TatI w'GTAC_w
Cuts at: 3345 3345
Size: 3830
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 112 144 3347 3379 112
Size: 32 3203 32 563
Fragments arranged by size:
3203 563 32 32
Tth111I GACn'n_nGTC
Cuts at: 1627 1627
Size: 3830
XbaI T'CTAG_A
Cuts at: 861 861
Size: 3830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 120 1325 120
Size: 1205 2625
XmaI C'CCGG_G
Cuts at: 850 850
Size: 3830
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsmI BsmBI BspEI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I CspCI DraIII EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PsiI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI