Restriction map for
pF1KB5018
(Circular) (Six-base) MAPSORT of: pF1KB5018.seq Check: 7708 from: 1 to: 4199
pF1KB5018 4199 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 462 831 462
Size: 369 3830
AatII G_ACGT'C
Cuts at: 505 505
Size: 4199
AccI GT'mk_AC
Cuts at: 1237 1237
Size: 4199
Acc65I G'GTAC_C
Cuts at: 358 1215 358
Size: 857 3342
AclI AA'CG_TT
Cuts at: 4039 4039
Size: 4199
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 521 2024 2456 2809 3471 521
Size: 1503 432 353 662 1249
Fragments arranged by size:
1503 1249 662 432 353
AflIII A'CryG_T
Cuts at: 1551 3351 3632 1551
Size: 1800 281 2118
Fragments arranged by size:
2118 1800 281
AleI CACnn'nnGTG
Cuts at: 577 577
Size: 4199
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 875 875
Size: 4199
AlwNI CAG_nnn'CTG
Cuts at: 514 526 888 954 1504 2942 514
Size: 12 362 66 550 1438 1771
Fragments arranged by size:
1771 1438 550 362 66 12
ApaLI G'TGCA_C
Cuts at: 3037 3037
Size: 4199
ApoI r'AATT_y
Cuts at: 710 1203 3552 3808 710
Size: 493 2349 256 1101
Fragments arranged by size:
2349 1101 493 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4199
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4199
AvaI C'yCGr_G
Cuts at: 724 1219 724
Size: 495 3704
BamHI G'GATC_C
Cuts at: 1224 1224
Size: 4199
BanI G'GyrC_C
Cuts at: 358 929 1009 1215 1629 1877 1912 358
Size: 571 80 206 414 248 35 2645
Fragments arranged by size:
2645 571 414 248 206 80 35
BanII G_rGCy'C
Cuts at: 478 589 724 965 1213 2243 478
Size: 111 135 241 248 1030 2434
Fragments arranged by size:
2434 1030 248 241 135 111
BbeI G_GCGC'C
Cuts at: 1013 1633 1881 1013
Size: 620 248 3331
Fragments arranged by size:
3331 620 248
BbsI GAAGACnn'nnnn_
Cuts at: 566 566
Size: 4199
BbvCI CC'TCA_GC
Cuts at: 363 905 363
Size: 542 3657
BciVI GTATCCnnnnn_n'
Cuts at: 2097 3153 4199 2097
Size: 1056 1046 2097
Fragments arranged by size:
2097 1056 1046
BclI T'GATC_A
Cuts at: 1719 1719
Size: 4199
BglII A'GATC_T
Cuts at: 266 1714 3751 266
Size: 1448 2037 714
Fragments arranged by size:
2037 1448 714
BlpI GC'TnA_GC
Cuts at: 1311 1311
Size: 4199
Bme1580I G_kGCm'C
Cuts at: 1824 1917 3041 1824
Size: 93 1124 2982
Fragments arranged by size:
2982 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 816 1411 1492 1827 3971 816
Size: 595 81 335 2144 1044
Fragments arranged by size:
2144 1044 595 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2566 2566
Size: 4199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 820 865 820
Size: 45 4154
Bpu10I CC'TnA_GC
Cuts at: 363 905 363
Size: 542 3657
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 291 1375 2254 2968 3266 291
Size: 1084 879 714 298 1224
Fragments arranged by size:
1224 1084 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3903 3903
Size: 4199
BsaAI yAC'GTr
Cuts at: 1552 1552
Size: 4199
BsaBI GATnn'nnATC
Cuts at: 6 1713 3750 6
Size: 1707 2037 455
Fragments arranged by size:
2037 1707 455
BsaHI Gr'CG_yC
Cuts at: 502 1010 1054 1630 1878 2580 4076 502
Size: 508 44 576 248 702 1496 625
Fragments arranged by size:
1496 702 625 576 508 248 44
BsaWI w'CCGG_w
Cuts at: 1388 1909 2587 2998 3145 3419 1388
Size: 521 678 411 147 274 2168
Fragments arranged by size:
2168 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 553 583 604 634 553
Size: 30 21 30 4118
Fragments arranged by size:
4118 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1036 1036
Size: 4199
BseYI C'CCAG_C
Cuts at: 888 1624 2382 3047 3455 888
Size: 736 758 665 408 1632
Fragments arranged by size:
1632 758 736 665 408
BsiEI CG_ry'CG
Cuts at: 104 1741 1787 3017 104
Size: 1637 46 1230 1286
Fragments arranged by size:
1637 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1213 1991 2181 3041 1213
Size: 778 190 860 2371
Fragments arranged by size:
2371 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 169 592 169
Size: 423 3776
Bsp1286I G_dGCh'C
Cuts at: 478 589 724 965 1213 1824 1917 1991 2181
Size: 111 135 241 248 611 93 74 190
Cuts at: 2181 2243 3041 478
Size: 62 798 1636
Fragments arranged by size:
1636 798 611 248 241 190 135 111
93 74 62
BspHI T'CATG_A
Cuts at: 2631 2631
Size: 4199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 462 831 1249 1463 1765 2146 462
Size: 369 418 214 302 381 2515
Fragments arranged by size:
2515 418 381 369 302 214
BsrBI CCG'CTC
Cuts at: 50 642 2491 2545 4029 4194 50
Size: 592 1849 54 1484 165 55
Fragments arranged by size:
1849 1484 592 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2111 2111
Size: 4199
BsrFI r'CCGG_y
Cuts at: 259 349 771 926 2197 2378 259
Size: 90 422 155 1271 181 2080
Fragments arranged by size:
2080 1271 422 181 155 90
BssSI C'ACGA_G
Cuts at: 2470 3178 2470
Size: 708 3491
BstAPI GCAn_nnn'nTGC
Cuts at: 1576 1576
Size: 4199
BstBI TT'CG_AA
Cuts at: 2560 2560
Size: 4199
BstEII G'GTnAC_C
Cuts at: 333 333
Size: 4199
BstYI r'GATC_y
Cuts at: 1 155 266 1224 1691 1714 2049 2295 2601
Size: 154 111 958 467 23 335 246 306
Cuts at: 2601 2613 2699 3751 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 958 467 449 335 306 246 154
111 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 432 432
Size: 4199
BtgI C'CryG_G
Cuts at: 107 233 623 689 776 107
Size: 126 390 66 87 3530
Fragments arranged by size:
3530 390 126 87 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2149 2330 2493 2149
Size: 181 163 3855
Fragments arranged by size:
3855 181 163
BtsI GCAGTG_nn'
Cuts at: 1442 1442
Size: 4199
ClaI AT'CG_AT
Cuts at: 305 305
Size: 4199
DraI TTT'AAA
Cuts at: 1198 1198
Size: 4199
DrdI GACnn_nn'nnGTC
Cuts at: 1905 3249 1905
Size: 1344 2855
EaeI y'GGCC_r
Cuts at: 110 351 650 773 1784 1958 2349 2376 3721
Size: 241 299 123 1011 174 391 27 1345
Cuts at: 3721 110
Size: 588
Fragments arranged by size:
1345 1011 588 391 299 241 174 123
27
EagI C'GGCC_G
Cuts at: 1784 1784
Size: 4199
EarI CTCTTCn'nnn_
Cuts at: 540 2222 2432 540
Size: 1682 210 2307
Fragments arranged by size:
2307 1682 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 480 994 2407 3149 3295 4009 480
Size: 514 1413 742 146 714 670
Fragments arranged by size:
1413 742 714 670 514 146
EcoICRI GAG'CTC
Cuts at: 1211 1211
Size: 4199
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 521 820 865 2024 2456 2809 3471 521
Size: 299 45 1159 432 353 662 1249
Fragments arranged by size:
1249 1159 662 432 353 299 45
EcoO109I rG'GnC_Cy
Cuts at: 1026 1089 1338 1026
Size: 63 249 3887
Fragments arranged by size:
3887 249 63
EcoRI G'AATT_C
Cuts at: 710 1203 710
Size: 493 3706
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2218 2218
Size: 4199
FspI TGC'GCA
Cuts at: 1980 1980
Size: 4199
HaeII r_GCGC'y
Cuts at: 1013 1633 1881 3111 3882 1013
Size: 620 248 1230 771 1330
Fragments arranged by size:
1330 1230 771 620 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 233 265 502 534 553 585 604 629 636
Size: 32 237 32 19 32 19 25 7
Cuts at: 636 661 2041 2071 2073 2103 233
Size: 25 1380 30 2 30 2329
Fragments arranged by size:
2329 1380 237 32 32 32 30 30
25 25 19 19 7 2
HincII GTy'rAC
Cuts at: 1238 1238
Size: 4199
KasI G'GCGC_C
Cuts at: 1009 1629 1877 1009
Size: 620 248 3331
Fragments arranged by size:
3331 620 248
KpnI G_GTAC'C
Cuts at: 362 1219 362
Size: 857 3342
MluI A'CGCG_T
Cuts at: 3632 3632
Size: 4199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2958 3142 2958
Size: 184 4015
MscI TGG'CCA
Cuts at: 1960 1960
Size: 4199
MslI CAynn'nnrTG
Cuts at: 577 622 949 1048 2188 2315 577
Size: 45 327 99 1140 127 2461
Fragments arranged by size:
2461 1140 327 127 99 45
MspA1I CmG'CkG
Cuts at: 391 548 888 1310 1624 1984 2768 3013 391
Size: 157 340 422 314 360 784 245 1577
Fragments arranged by size:
1577 784 422 360 340 314 245 157
NaeI GCC'GGC
Cuts at: 261 351 773 928 2380 261
Size: 90 422 155 1452 2080
Fragments arranged by size:
2080 1452 422 155 90
NarI GG'CG_CC
Cuts at: 1010 1630 1878 1010
Size: 620 248 3331
Fragments arranged by size:
3331 620 248
NcoI C'CATG_G
Cuts at: 107 623 107
Size: 516 3683
NgoMIV G'CCGG_C
Cuts at: 259 349 771 926 2378 259
Size: 90 422 155 1452 2080
Fragments arranged by size:
2080 1452 422 155 90
NspI r_CATG'y
Cuts at: 441 837 1252 1752 3355 441
Size: 396 415 500 1603 1285
Fragments arranged by size:
1603 1285 500 415 396
PasI CC'CwG_GG
Cuts at: 967 1092 967
Size: 125 4074
PciI A'CATG_T
Cuts at: 3351 3351
Size: 4199
PflMI CCAn_nnn'nTGG
Cuts at: 888 888
Size: 4199
PfoI T'CCnGG_A
Cuts at: 746 746
Size: 4199
PmeI GTTT'AAAC
Cuts at: 1198 1198
Size: 4199
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2645 2677 2645
Size: 32 4167
PstI C_TGCA'G
Cuts at: 140 1246 1931 140
Size: 1106 685 2408
Fragments arranged by size:
2408 1106 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4199
PvuII CAG'CTG
Cuts at: 391 888 1624 1984 391
Size: 497 736 360 2606
Fragments arranged by size:
2606 736 497 360
RsrII CG'GwC_CG
Cuts at: 2394 2394
Size: 4199
SacI G_AGCT'C
Cuts at: 1213 1213
Size: 4199
SalI G'TCGA_C
Cuts at: 1236 1236
Size: 4199
SapI GCTCTTCn'nnn_
Cuts at: 2222 2432 2222
Size: 210 3989
SbfI CC_TGCA'GG
Cuts at: 1246 1246
Size: 4199
ScaI AGT'ACT
Cuts at: 3716 3716
Size: 4199
SfcI C'TryA_G
Cuts at: 33 136 1073 1242 1927 2895 3086 33
Size: 103 937 169 685 968 191 1146
Fragments arranged by size:
1146 968 937 685 191 169 103
SfoI GGC'GCC
Cuts at: 1011 1631 1879 1011
Size: 620 248 3331
Fragments arranged by size:
3331 620 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4199
SmaI CCC'GGG
Cuts at: 1221 1221
Size: 4199
SmlI C'TyrA_G
Cuts at: 270 1354 2269 2983 3245 270
Size: 1084 915 714 262 1224
Fragments arranged by size:
1224 1084 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4199
SphI G_CATG'C
Cuts at: 1252 1752 1252
Size: 500 3699
StuI AGG'CCT
Cuts at: 379 1123 379
Size: 744 3455
StyI C'CwwG_G
Cuts at: 107 623 1333 1687 107
Size: 516 710 354 2619
Fragments arranged by size:
2619 710 516 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 399 1782 1915 2374 2583 399
Size: 1383 133 459 209 2015
Fragments arranged by size:
2015 1383 459 209 133
TatI w'GTAC_w
Cuts at: 3714 3714
Size: 4199
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 696 728 3716 3748 696
Size: 32 2988 32 1147
Fragments arranged by size:
2988 1147 32 32
Tth111I GACn'n_nGTC
Cuts at: 280 1996 280
Size: 1716 2483
XbaI T'CTAG_A
Cuts at: 1230 1230
Size: 4199
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1694 1694
Size: 4199
XmaI C'CCGG_G
Cuts at: 1219 1219
Size: 4199
ZraI GAC'GTC
Cuts at: 503 503
Size: 4199
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AloI ApaI AscI AvrII
BaeI BcgI BfrBI BglI BmgBI BmtI BsgI BsiWI
BsmI BspEI BsrGI BssHII BstXI BstZ17I CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI