Restriction map for
pF1KB5016
(Circular) (Six-base) MAPSORT of: pF1KB5016.seq Check: 4331 from: 1 to: 5144
pF1KB5016 5144 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AatII G_ACGT'C
Cuts at: 311 311
Size: 5144
AccI GT'mk_AC
Cuts at: 2182 2182
Size: 5144
Acc65I G'GTAC_C
Cuts at: 2160 2160
Size: 5144
AclI AA'CG_TT
Cuts at: 4984 4984
Size: 5144
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 575 707 1094 2969 3401 3754 4416 310
Size: 265 132 387 1875 432 353 662 1038
Fragments arranged by size:
1875 1038 662 432 387 353 265 132
AfeI AGC'GCT
Cuts at: 255 1189 255
Size: 934 4210
AflIII A'CryG_T
Cuts at: 2496 4296 4577 2496
Size: 1800 281 3063
Fragments arranged by size:
3063 1800 281
AleI CACnn'nnGTG
Cuts at: 1445 1445
Size: 5144
AlwNI CAG_nnn'CTG
Cuts at: 2449 3887 2449
Size: 1438 3706
ApaI G_GGCC'C
Cuts at: 1168 1168
Size: 5144
ApaLI G'TGCA_C
Cuts at: 3982 3982
Size: 5144
ApoI r'AATT_y
Cuts at: 1788 2122 2148 4497 4753 1788
Size: 334 26 2349 256 2179
Fragments arranged by size:
2349 2179 334 256 26
AseI AT'TA_AT
Cuts at: 20 20
Size: 5144
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5144
AvaI C'yCGr_G
Cuts at: 1747 2164 1747
Size: 417 4727
BamHI G'GATC_C
Cuts at: 2169 2169
Size: 5144
BanI G'GyrC_C
Cuts at: 2160 2574 2822 2857 2160
Size: 414 248 35 4447
Fragments arranged by size:
4447 414 248 35
BanII G_rGCy'C
Cuts at: 426 518 1168 1224 1545 2158 3188 426
Size: 92 650 56 321 613 1030 2382
Fragments arranged by size:
2382 1030 650 613 321 92 56
BbeI G_GCGC'C
Cuts at: 2578 2826 2578
Size: 248 4896
BbsI GAAGACnn'nnnn_
Cuts at: 298 571 796 994 298
Size: 273 225 198 4448
Fragments arranged by size:
4448 273 225 198
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5110
BciVI GTATCCnnnnn_n'
Cuts at: 3042 4098 5144 3042
Size: 1056 1046 3042
Fragments arranged by size:
3042 1056 1046
BclI T'GATC_A
Cuts at: 2664 2664
Size: 5144
BglI GCCn_nnn'nGGC
Cuts at: 2135 2135
Size: 5144
BglII A'GATC_T
Cuts at: 2659 4696 2659
Size: 2037 3107
BlpI GC'TnA_GC
Cuts at: 2256 2256
Size: 5144
Bme1580I G_kGCm'C
Cuts at: 494 1168 2769 2862 3986 494
Size: 674 1601 93 1124 1652
Fragments arranged by size:
1652 1601 1124 674 93
BmrI ACTGGGnnnn_n'
Cuts at: 513 1860 2356 2437 2772 4916 513
Size: 1347 496 81 335 2144 741
Fragments arranged by size:
2144 1347 741 496 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 246 3511 246
Size: 3265 1879
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 387 1198 1678 387
Size: 811 480 3853
Fragments arranged by size:
3853 811 480
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 974 1360 2320 3199 3913 4211 974
Size: 386 960 879 714 298 1907
Fragments arranged by size:
1907 960 879 714 386 298
BsaI GGTCTCn'nnnn_
Cuts at: 362 1562 4848 362
Size: 1200 3286 658
Fragments arranged by size:
3286 1200 658
BsaAI yAC'GTr
Cuts at: 1387 2497 1387
Size: 1110 4034
BsaBI GATnn'nnATC
Cuts at: 6 2658 4695 6
Size: 2652 2037 455
Fragments arranged by size:
2652 2037 455
BsaHI Gr'CG_yC
Cuts at: 308 1157 2575 2823 3525 5021 308
Size: 849 1418 248 702 1496 431
Fragments arranged by size:
1496 1418 849 702 431 248
BsaWI w'CCGG_w
Cuts at: 2333 2854 3532 3943 4090 4364 2333
Size: 521 678 411 147 274 3113
Fragments arranged by size:
3113 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 385 415 385
Size: 30 5114
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 448 616 619 622 625 754 844 981 1057
Size: 168 3 3 3 129 90 137 76
Cuts at: 1057 1255 1270 1771 2002 448
Size: 198 15 501 231 3590
Fragments arranged by size:
3590 501 231 198 168 137 129 90
76 15 3 3 3
BseYI C'CCAG_C
Cuts at: 372 1276 2569 3327 3992 4400 372
Size: 904 1293 758 665 408 1116
Fragments arranged by size:
1293 1116 904 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2686 2732 3962 104
Size: 2582 46 1230 1286
Fragments arranged by size:
2582 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2002 2158 2936 3126 3986 2002
Size: 156 778 190 860 3160
Fragments arranged by size:
3160 860 778 190 156
BsmI GAATG_Cn'
Cuts at: 777 777
Size: 5144
Bsp1286I G_dGCh'C
Cuts at: 426 494 518 1168 1224 1545 2002 2158 2769
Size: 68 24 650 56 321 457 156 611
Cuts at: 2769 2862 2936 3126 3188 3986 426
Size: 93 74 190 62 798 1584
Fragments arranged by size:
1584 798 650 611 457 321 190 156
93 74 68 62 56 24
BspHI T'CATG_A
Cuts at: 3576 3576
Size: 5144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1580 2194 2408 2710 3091 1580
Size: 614 214 302 381 3633
Fragments arranged by size:
3633 614 381 302 214
BsrBI CCG'CTC
Cuts at: 50 508 3436 3490 4974 5139 50
Size: 458 2928 54 1484 165 55
Fragments arranged by size:
2928 1484 458 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2096 3056 2096
Size: 960 4184
BsrFI r'CCGG_y
Cuts at: 1992 3142 3323 1992
Size: 1150 181 3813
Fragments arranged by size:
3813 1150 181
BssSI C'ACGA_G
Cuts at: 394 942 3415 4123 394
Size: 548 2473 708 1415
Fragments arranged by size:
2473 1415 708 548
BstAPI GCAn_nnn'nTGC
Cuts at: 2521 2521
Size: 5144
BstBI TT'CG_AA
Cuts at: 3505 3505
Size: 5144
BstXI CCAn_nnnn'nTGG
Cuts at: 174 823 1553 174
Size: 649 730 3765
Fragments arranged by size:
3765 730 649
BstYI r'GATC_y
Cuts at: 1 2169 2636 2659 2994 3240 3546 3558 3644
Size: 2168 467 23 335 246 306 12 86
Cuts at: 3644 4696 1
Size: 1052 449
Fragments arranged by size:
2168 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1169 1169
Size: 5144
BtgI C'CryG_G
Cuts at: 143 383 960 143
Size: 240 577 4327
Fragments arranged by size:
4327 577 240
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3094 3275 3438 3094
Size: 181 163 4800
Fragments arranged by size:
4800 181 163
BtsI GCAGTG_nn'
Cuts at: 660 2387 660
Size: 1727 3417
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1477 1512 1477
Size: 35 5109
DraI TTT'AAA
Cuts at: 2143 2143
Size: 5144
DraIII CAC_nnn'GTG
Cuts at: 1492 1492
Size: 5144
DrdI GACnn_nn'nnGTC
Cuts at: 547 2044 2850 4194 547
Size: 1497 806 1344 1497
Fragments arranged by size:
1497 1497 1344 806
EaeI y'GGCC_r
Cuts at: 749 1061 2729 2903 3294 3321 4666 749
Size: 312 1668 174 391 27 1345 1227
Fragments arranged by size:
1668 1345 1227 391 312 174 27
EagI C'GGCC_G
Cuts at: 2729 2729
Size: 5144
EarI CTCTTCn'nnn_
Cuts at: 577 653 668 802 1322 1409 1648 1696 1716
Size: 76 15 134 520 87 239 48 20
Cuts at: 1716 1858 3167 3377 577
Size: 142 1309 210 2344
Fragments arranged by size:
2344 1309 520 239 210 142 134 87
76 48 20 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3352 4094 4240 4954 3352
Size: 742 146 714 3542
Fragments arranged by size:
3542 742 714 146
EcoICRI GAG'CTC
Cuts at: 2156 2156
Size: 5144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 387 575 707 1094 1198 1678 2969 3401
Size: 77 188 132 387 104 480 1291 432
Cuts at: 3401 3754 4416 310
Size: 353 662 1038
Fragments arranged by size:
1291 1038 662 480 432 387 353 188
132 104 77
EcoNI CCTnn'n_nnAGG
Cuts at: 405 2053 405
Size: 1648 3496
EcoO109I rG'GnC_Cy
Cuts at: 225 983 1164 1165 1173 1335 1881 2283 225
Size: 758 181 1 8 162 546 402 3086
Fragments arranged by size:
3086 758 546 402 181 162 8 1
EcoRI G'AATT_C
Cuts at: 2148 2148
Size: 5144
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1809 3163 1809
Size: 1354 3790
FspI TGC'GCA
Cuts at: 2925 2925
Size: 5144
HaeII r_GCGC'y
Cuts at: 257 1191 2578 2826 4056 4827 257
Size: 934 1387 248 1230 771 574
Fragments arranged by size:
1387 1230 934 771 574 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 516 548 904 936 970 1002 2986 3016 3018
Size: 32 356 32 34 32 1984 30 2
Cuts at: 3018 3048 516
Size: 30 2612
Fragments arranged by size:
2612 1984 356 34 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2183 2183
Size: 5144
KasI G'GCGC_C
Cuts at: 2574 2822 2574
Size: 248 4896
KpnI G_GTAC'C
Cuts at: 2164 2164
Size: 5144
MluI A'CGCG_T
Cuts at: 4577 4577
Size: 5144
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 563 3903 4087 563
Size: 3340 184 1620
Fragments arranged by size:
3340 1620 184
MscI TGG'CCA
Cuts at: 751 1063 2905 751
Size: 312 1842 2990
Fragments arranged by size:
2990 1842 312
MslI CAynn'nnrTG
Cuts at: 172 1307 1445 3133 3260 172
Size: 1135 138 1688 127 2056
Fragments arranged by size:
2056 1688 1135 138 127
MspA1I CmG'CkG
Cuts at: 376 1642 1960 2255 2569 2929 3713 3958 376
Size: 1266 318 295 314 360 784 245 1562
Fragments arranged by size:
1562 1266 784 360 318 314 295 245
NaeI GCC'GGC
Cuts at: 3325 3325
Size: 5144
NarI GG'CG_CC
Cuts at: 2575 2823 2575
Size: 248 4896
NgoMIV G'CCGG_C
Cuts at: 3323 3323
Size: 5144
NspI r_CATG'y
Cuts at: 769 2197 2697 4300 769
Size: 1428 500 1603 1613
Fragments arranged by size:
1613 1603 1428 500
PasI CC'CwG_GG
Cuts at: 427 1546 427
Size: 1119 4025
PciI A'CATG_T
Cuts at: 4296 4296
Size: 5144
PmeI GTTT'AAAC
Cuts at: 2143 2143
Size: 5144
PmlI CAC'GTG
Cuts at: 1387 1387
Size: 5144
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1408 1440 3590 3622 1408
Size: 32 2150 32 2930
Fragments arranged by size:
2930 2150 32 32
PpuMI rG'GwC_Cy
Cuts at: 225 983 1173 1881 225
Size: 758 190 708 3488
Fragments arranged by size:
3488 758 708 190
PspOMI G'GGCC_C
Cuts at: 1164 1164
Size: 5144
PstI C_TGCA'G
Cuts at: 1577 2191 2876 1577
Size: 614 685 3845
Fragments arranged by size:
3845 685 614
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5144
PvuII CAG'CTG
Cuts at: 376 1642 1960 2569 2929 376
Size: 1266 318 609 360 2591
Fragments arranged by size:
2591 1266 609 360 318
RsrII CG'GwC_CG
Cuts at: 3339 3339
Size: 5144
SacI G_AGCT'C
Cuts at: 2158 2158
Size: 5144
SalI G'TCGA_C
Cuts at: 2181 2181
Size: 5144
SanDI GG'GwC_CC
Cuts at: 1881 1881
Size: 5144
SapI GCTCTTCn'nnn_
Cuts at: 577 668 1696 3167 3377 577
Size: 91 1028 1471 210 2344
Fragments arranged by size:
2344 1471 1028 210 91
SbfI CC_TGCA'GG
Cuts at: 1577 2191 1577
Size: 614 4530
ScaI AGT'ACT
Cuts at: 4661 4661
Size: 5144
SfcI C'TryA_G
Cuts at: 33 1101 1191 1573 1944 2104 2187 2872 3840
Size: 1068 90 382 371 160 83 685 968
Cuts at: 3840 4031 33
Size: 191 1146
Fragments arranged by size:
1146 1068 968 685 382 371 191 160
90 83
SfoI GGC'GCC
Cuts at: 2576 2824 2576
Size: 248 4896
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5144
SmaI CCC'GGG
Cuts at: 2166 2166
Size: 5144
SmlI C'TyrA_G
Cuts at: 989 1339 1747 2299 3214 3928 4190 989
Size: 350 408 552 915 714 262 1943
Fragments arranged by size:
1943 915 714 552 408 350 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5144
SphI G_CATG'C
Cuts at: 2197 2697 2197
Size: 500 4644
StyI C'CwwG_G
Cuts at: 444 2278 2632 444
Size: 1834 354 2956
Fragments arranged by size:
2956 1834 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 276 1420 2017 2727 2860 3319 3528 276
Size: 1144 597 710 133 459 209 1892
Fragments arranged by size:
1892 1144 710 597 459 209 133
TatI w'GTAC_w
Cuts at: 4659 4659
Size: 5144
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 385 417 1165 1197 4661 4693 385
Size: 32 748 32 3464 32 836
Fragments arranged by size:
3464 836 748 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2941 2941
Size: 5144
XbaI T'CTAG_A
Cuts at: 2175 2175
Size: 5144
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 924 1493 2639 924
Size: 569 1146 3429
Fragments arranged by size:
3429 1146 569
XhoI C'TCGA_G
Cuts at: 1747 1747
Size: 5144
XmaI C'CCGG_G
Cuts at: 2164 2164
Size: 5144
XmnI GAAnn'nnTTC
Cuts at: 1423 1789 1792 1423
Size: 366 3 4775
Fragments arranged by size:
4775 366 3
ZraI GAC'GTC
Cuts at: 309 309
Size: 5144
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AflII AgeI AhdI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsgI BsiWI
BsmBI BspEI BsrGI BssHII BstEII BstZ17I ClaI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI