Restriction map for
pF1KB5014
(Circular) (Six-base) MAPSORT of: pF1KB5014.seq Check: 6444 from: 1 to: 4364
pF1KB5014 4364 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 170 1402 170
Size: 1232 3132
Acc65I G'GTAC_C
Cuts at: 1380 1380
Size: 4364
AclI AA'CG_TT
Cuts at: 4204 4204
Size: 4364
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2189 2621 2974 3636 2189
Size: 432 353 662 2917
Fragments arranged by size:
2917 662 432 353
AflII C'TTAA_G
Cuts at: 1207 1207
Size: 4364
AflIII A'CryG_T
Cuts at: 1716 3516 3797 1716
Size: 1800 281 2283
Fragments arranged by size:
2283 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 586 586
Size: 4364
AlwNI CAG_nnn'CTG
Cuts at: 189 378 1669 3107 189
Size: 189 1291 1438 1446
Fragments arranged by size:
1446 1438 1291 189
ApaLI G'TGCA_C
Cuts at: 3202 3202
Size: 4364
ApoI r'AATT_y
Cuts at: 250 1368 3717 3973 250
Size: 1118 2349 256 641
Fragments arranged by size:
2349 1118 641 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4364
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4364
AvaI C'yCGr_G
Cuts at: 823 856 997 1384 823
Size: 33 141 387 3803
Fragments arranged by size:
3803 387 141 33
BamHI G'GATC_C
Cuts at: 735 974 1389 735
Size: 239 415 3710
Fragments arranged by size:
3710 415 239
BanI G'GyrC_C
Cuts at: 526 1078 1380 1794 2042 2077 526
Size: 552 302 414 248 35 2813
Fragments arranged by size:
2813 552 414 302 248 35
BanII G_rGCy'C
Cuts at: 1378 2408 1378
Size: 1030 3334
BbeI G_GCGC'C
Cuts at: 1798 2046 1798
Size: 248 4116
BbsI GAAGACnn'nnnn_
Cuts at: 704 764 1229 704
Size: 60 465 3839
Fragments arranged by size:
3839 465 60
BbvCI CC'TCA_GC
Cuts at: 1124 1124
Size: 4364
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4330
BciVI GTATCCnnnnn_n'
Cuts at: 2262 3318 4364 2262
Size: 1056 1046 2262
Fragments arranged by size:
2262 1056 1046
BclI T'GATC_A
Cuts at: 575 1884 575
Size: 1309 3055
BglI GCCn_nnn'nGGC
Cuts at: 143 143
Size: 4364
BglII A'GATC_T
Cuts at: 1879 3916 1879
Size: 2037 2327
BlpI GC'TnA_GC
Cuts at: 1476 1476
Size: 4364
Bme1580I G_kGCm'C
Cuts at: 970 1989 2082 3206 970
Size: 1019 93 1124 2128
Fragments arranged by size:
2128 1124 1019 93
BmgBI CAC'GTC
Cuts at: 1145 1145
Size: 4364
BmrI ACTGGGnnnn_n'
Cuts at: 771 822 873 892 1010 1576 1657 1992 4136
Size: 51 51 19 118 566 81 335 2144
Cuts at: 4136 771
Size: 999
Fragments arranged by size:
2144 999 566 335 118 81 51 51
19
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 705 2731 705
Size: 2026 2338
Bpu10I CC'TnA_GC
Cuts at: 1124 1124
Size: 4364
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1540 2419 3133 3431 1540
Size: 879 714 298 2473
Fragments arranged by size:
2473 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4068 4068
Size: 4364
BsaAI yAC'GTr
Cuts at: 1717 1717
Size: 4364
BsaBI GATnn'nnATC
Cuts at: 6 1878 3915 6
Size: 1872 2037 455
Fragments arranged by size:
2037 1872 455
BsaHI Gr'CG_yC
Cuts at: 1795 2043 2745 4241 1795
Size: 248 702 1496 1918
Fragments arranged by size:
1918 1496 702 248
BsaWI w'CCGG_w
Cuts at: 753 1553 2074 2752 3163 3310 3584 753
Size: 800 521 678 411 147 274 1533
Fragments arranged by size:
1533 800 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 301 1141 1210 1228 1255 1258 1261 1306
Size: 171 840 69 18 27 3 3 45
Cuts at: 1306 1315 1336 130
Size: 9 21 3158
Fragments arranged by size:
3158 840 171 69 45 27 21 18
9 3 3
BseYI C'CCAG_C
Cuts at: 1047 1789 2547 3212 3620 1047
Size: 742 758 665 408 1791
Fragments arranged by size:
1791 758 742 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 406
Size: 4364
BsiEI CG_ry'CG
Cuts at: 104 1906 1952 3182 104
Size: 1802 46 1230 1286
Fragments arranged by size:
1802 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1378 2156 2346 3206 1378
Size: 778 190 860 2536
Fragments arranged by size:
2536 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 191 1135 191
Size: 944 3420
Bsp1286I G_dGCh'C
Cuts at: 970 1378 1989 2082 2156 2346 2408 3206 970
Size: 408 611 93 74 190 62 798 2128
Fragments arranged by size:
2128 798 611 408 190 93 74 62
BspHI T'CATG_A
Cuts at: 2796 2796
Size: 4364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1056 1414 1628 1930 2311 1056
Size: 358 214 302 381 3109
Fragments arranged by size:
3109 381 358 302 214
BsrBI CCG'CTC
Cuts at: 50 771 2656 2710 4194 4359 50
Size: 721 1885 54 1484 165 55
Fragments arranged by size:
1885 1484 721 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2276 2276
Size: 4364
BsrFI r'CCGG_y
Cuts at: 2362 2543 2362
Size: 181 4183
BsrGI T'GTAC_A
Cuts at: 620 620
Size: 4364
BssSI C'ACGA_G
Cuts at: 2635 3343 2635
Size: 708 3656
BstAPI GCAn_nnn'nTGC
Cuts at: 1741 1741
Size: 4364
BstBI TT'CG_AA
Cuts at: 2725 2725
Size: 4364
BstYI r'GATC_y
Cuts at: 1 735 974 1389 1856 1879 2214 2460 2766
Size: 734 239 415 467 23 335 246 306
Cuts at: 2766 2778 2864 3916 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 734 467 449 415 335 306 246
239 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 806 806
Size: 4364
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2314 2495 2658 2314
Size: 181 163 4020
Fragments arranged by size:
4020 181 163
BtsI GCAGTG_nn'
Cuts at: 1607 1607
Size: 4364
ClaI AT'CG_AT
Cuts at: 782 782
Size: 4364
DraI TTT'AAA
Cuts at: 1363 1363
Size: 4364
DrdI GACnn_nn'nnGTC
Cuts at: 2070 3414 2070
Size: 1344 3020
EaeI y'GGCC_r
Cuts at: 149 341 637 1338 1949 2123 2514 2541 3886
Size: 192 296 701 611 174 391 27 1345
Cuts at: 3886 149
Size: 627
Fragments arranged by size:
1345 701 627 611 391 296 192 174
27
EagI C'GGCC_G
Cuts at: 1949 1949
Size: 4364
EarI CTCTTCn'nnn_
Cuts at: 866 2387 2597 866
Size: 1521 210 2633
Fragments arranged by size:
2633 1521 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2572 3314 3460 4174 2572
Size: 742 146 714 2762
Fragments arranged by size:
2762 742 714 146
EcoICRI GAG'CTC
Cuts at: 1376 1376
Size: 4364
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2189 2621 2974 3636 2189
Size: 432 353 662 2917
Fragments arranged by size:
2917 662 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 1063 1063
Size: 4364
EcoO109I rG'GnC_Cy
Cuts at: 1503 1503
Size: 4364
EcoRI G'AATT_C
Cuts at: 1368 1368
Size: 4364
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 4364
FspI TGC'GCA
Cuts at: 2145 2145
Size: 4364
HaeII r_GCGC'y
Cuts at: 1798 2046 3276 4047 1798
Size: 248 1230 771 2115
Fragments arranged by size:
2115 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 720 752 771 803 2206 2236 2238 2268 720
Size: 32 19 32 1403 30 2 30 2816
Fragments arranged by size:
2816 1403 32 32 30 30 19 2
HincII GTy'rAC
Cuts at: 1403 1403
Size: 4364
KasI G'GCGC_C
Cuts at: 1794 2042 1794
Size: 248 4116
KpnI G_GTAC'C
Cuts at: 1384 1384
Size: 4364
MluI A'CGCG_T
Cuts at: 3797 3797
Size: 4364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3123 3307 3123
Size: 184 4180
MscI TGG'CCA
Cuts at: 2125 2125
Size: 4364
MslI CAynn'nnrTG
Cuts at: 2353 2480 2353
Size: 127 4237
MspA1I CmG'CkG
Cuts at: 126 215 1475 1789 2149 2933 3178 126
Size: 89 1260 314 360 784 245 1312
Fragments arranged by size:
1312 1260 784 360 314 245 89
NaeI GCC'GGC
Cuts at: 2545 2545
Size: 4364
NarI GG'CG_CC
Cuts at: 1795 2043 1795
Size: 248 4116
NgoMIV G'CCGG_C
Cuts at: 2543 2543
Size: 4364
NspI r_CATG'y
Cuts at: 474 1417 1917 3520 474
Size: 943 500 1603 1318
Fragments arranged by size:
1603 1318 943 500
PciI A'CATG_T
Cuts at: 3516 3516
Size: 4364
PflMI CCAn_nnn'nTGG
Cuts at: 378 378
Size: 4364
PmeI GTTT'AAAC
Cuts at: 1363 1363
Size: 4364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2810 2842 2810
Size: 32 4332
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 901 933 901
Size: 32 4332
PstI C_TGCA'G
Cuts at: 1411 2096 1411
Size: 685 3679
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4364
PvuII CAG'CTG
Cuts at: 1789 2149 1789
Size: 360 4004
RsrII CG'GwC_CG
Cuts at: 2559 2559
Size: 4364
SacI G_AGCT'C
Cuts at: 1378 1378
Size: 4364
SalI G'TCGA_C
Cuts at: 1401 1401
Size: 4364
SapI GCTCTTCn'nnn_
Cuts at: 2387 2597 2387
Size: 210 4154
SbfI CC_TGCA'GG
Cuts at: 1411 1411
Size: 4364
ScaI AGT'ACT
Cuts at: 3881 3881
Size: 4364
SfcI C'TryA_G
Cuts at: 33 1407 2092 3060 3251 33
Size: 1374 685 968 191 1146
Fragments arranged by size:
1374 1146 968 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 143 143
Size: 4364
SfoI GGC'GCC
Cuts at: 1796 2044 1796
Size: 248 4116
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4364
SmaI CCC'GGG
Cuts at: 1386 1386
Size: 4364
SmlI C'TyrA_G
Cuts at: 1207 1519 2434 3148 3410 1207
Size: 312 915 714 262 2161
Fragments arranged by size:
2161 915 714 312 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4364
SphI G_CATG'C
Cuts at: 1417 1917 1417
Size: 500 3864
StyI C'CwwG_G
Cuts at: 692 1346 1498 1852 692
Size: 654 152 354 3204
Fragments arranged by size:
3204 654 354 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 735 995 1947 2080 2539 2748 735
Size: 260 952 133 459 209 2351
Fragments arranged by size:
2351 952 459 260 209 133
TatI w'GTAC_w
Cuts at: 620 917 3879 620
Size: 297 2962 1105
Fragments arranged by size:
2962 1105 297
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 871 903 3881 3913 871
Size: 32 2978 32 1322
Fragments arranged by size:
2978 1322 32 32
Tth111I GACn'n_nGTC
Cuts at: 2161 2161
Size: 4364
XbaI T'CTAG_A
Cuts at: 1395 1395
Size: 4364
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1353 1859 1353
Size: 506 3858
XmaI C'CCGG_G
Cuts at: 1384 1384
Size: 4364
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PmeI PpiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AloI ApaI
AscI AvrII BaeI BfrBI BmtI BpmI BsaXI BsiWI
BsmI BspEI BssHII BstEII BstXI BstZ17I BtgI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI SacII SanDI
SexAI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI