Restriction map for
pF1KB5013
(Circular) (Six-base) MAPSORT of: pF1KB5013.seq Check: 5563 from: 1 to: 3722
pF1KB5013 3722 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AatII G_ACGT'C
Cuts at: 172 172
Size: 3722
AccI GT'mk_AC
Cuts at: 760 760
Size: 3722
Acc65I G'GTAC_C
Cuts at: 738 738
Size: 3722
AclI AA'CG_TT
Cuts at: 3562 3562
Size: 3722
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 265 268 378 1547 1979 2726 2994 186
Size: 79 3 110 1169 432 747 268 914
Fragments arranged by size:
1169 914 747 432 268 110 79 3
AfeI AGC'GCT
Cuts at: 313 313
Size: 3722
AflIII A'CryG_T
Cuts at: 1074 2178 3155 1074
Size: 1104 977 1641
Fragments arranged by size:
1641 1104 977
AgeI A'CCGG_T
Cuts at: 527 2110 527
Size: 1583 2139
AlwNI CAG_nnn'CTG
Cuts at: 1027 2594 1027
Size: 1567 2155
ApaLI G'TGCA_C
Cuts at: 2492 2492
Size: 3722
ApoI r'AATT_y
Cuts at: 633 726 3075 3331 633
Size: 93 2349 256 1024
Fragments arranged by size:
2349 1024 256 93
AseI AT'TA_AT
Cuts at: 20 593 20
Size: 573 3149
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3722
AvaI C'yCGr_G
Cuts at: 742 742
Size: 3722
BamHI G'GATC_C
Cuts at: 747 747
Size: 3722
BanI G'GyrC_C
Cuts at: 459 738 1152 1400 1435 459
Size: 279 414 248 35 2746
Fragments arranged by size:
2746 414 279 248 35
BanII G_rGCy'C
Cuts at: 736 1766 736
Size: 1030 2692
BbeI G_GCGC'C
Cuts at: 1156 1404 1156
Size: 248 3474
BciVI GTATCCnnnnn_n'
Cuts at: 1620 2381 3722 1620
Size: 761 1341 1620
Fragments arranged by size:
1620 1341 761
BclI T'GATC_A
Cuts at: 1242 1242
Size: 3722
BglII A'GATC_T
Cuts at: 1237 3274 1237
Size: 2037 1685
BlpI GC'TnA_GC
Cuts at: 834 834
Size: 3722
Bme1580I G_kGCm'C
Cuts at: 464 1347 1440 2496 464
Size: 883 93 1056 1690
Fragments arranged by size:
1690 1056 883 93
BmrI ACTGGGnnnn_n'
Cuts at: 934 1015 1350 3494 934
Size: 81 335 2144 1162
Fragments arranged by size:
2144 1162 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2089 2089
Size: 3722
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 684 238
Size: 446 3276
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 898 1777 2269 2567 898
Size: 879 492 298 2053
Fragments arranged by size:
2053 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3426 3426
Size: 3722
BsaAI yAC'GTr
Cuts at: 1075 1075
Size: 3722
BsaBI GATnn'nnATC
Cuts at: 6 1236 3273 6
Size: 1230 2037 455
Fragments arranged by size:
2037 1230 455
BsaHI Gr'CG_yC
Cuts at: 152 169 1153 1401 2103 3599 152
Size: 17 984 248 702 1496 275
Fragments arranged by size:
1496 984 702 275 248 17
BsaWI w'CCGG_w
Cuts at: 527 911 1432 2110 2384 2531 2942 527
Size: 384 521 678 274 147 411 1307
Fragments arranged by size:
1307 678 521 411 384 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 443 473 443
Size: 30 3692
BseYI C'CCAG_C
Cuts at: 1147 1905 2482 2978 1147
Size: 758 577 496 1891
Fragments arranged by size:
1891 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 199
Size: 3722
BsiEI CG_ry'CG
Cuts at: 104 1264 1310 2518 104
Size: 1160 46 1208 1308
Fragments arranged by size:
1308 1208 1160 46
BsiHKAI G_wGCw'C
Cuts at: 736 1514 1704 2496 736
Size: 778 190 792 1962
Fragments arranged by size:
1962 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 464 736 1347 1440 1514 1704 1766 2496 464
Size: 272 611 93 74 190 62 730 1690
Fragments arranged by size:
1690 730 611 272 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2942 2942
Size: 3722
BspHI T'CATG_A
Cuts at: 2898 2898
Size: 3722
BspMI ACCTGCnnnn'nnnn_
Cuts at: 772 986 1288 1669 772
Size: 214 302 381 2825
Fragments arranged by size:
2825 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2014 2068 3552 3717 50
Size: 1964 54 1484 165 55
Fragments arranged by size:
1964 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1634 1634
Size: 3722
BsrFI r'CCGG_y
Cuts at: 456 527 1720 1901 2110 456
Size: 71 1193 181 209 2068
Fragments arranged by size:
2068 1193 209 181 71
BssSI C'ACGA_G
Cuts at: 1993 2351 1993
Size: 358 3364
BstAPI GCAn_nnn'nTGC
Cuts at: 1099 1099
Size: 3722
BstBI TT'CG_AA
Cuts at: 2083 2083
Size: 3722
BstYI r'GATC_y
Cuts at: 1 747 1214 1237 1572 1818 2830 2916 2928
Size: 746 467 23 335 246 1012 86 12
Cuts at: 2928 3274 1
Size: 346 449
Fragments arranged by size:
1012 746 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 221 107
Size: 114 3608
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1672 1853 2016 1672
Size: 181 163 3378
Fragments arranged by size:
3378 181 163
BtsI GCAGTG_nn'
Cuts at: 164 512 965 164
Size: 348 453 2921
Fragments arranged by size:
2921 453 348
DraI TTT'AAA
Cuts at: 721 721
Size: 3722
DrdI GACnn_nn'nnGTC
Cuts at: 1428 2286 1428
Size: 858 2864
EaeI y'GGCC_r
Cuts at: 476 1307 1481 1872 1899 3244 476
Size: 831 174 391 27 1345 954
Fragments arranged by size:
1345 954 831 391 174 27
EagI C'GGCC_G
Cuts at: 1307 1307
Size: 3722
EarI CTCTTCn'nnn_
Cuts at: 1745 1955 1745
Size: 210 3512
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1930 2240 2386 3532 1930
Size: 310 146 1146 2120
Fragments arranged by size:
2120 1146 310 146
EcoICRI GAG'CTC
Cuts at: 734 734
Size: 3722
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 238 265 268 378 684 1547 1979 2726
Size: 52 27 3 110 306 863 432 747
Cuts at: 2726 2994 186
Size: 268 914
Fragments arranged by size:
914 863 747 432 306 268 110 52
27 3
EcoO109I rG'GnC_Cy
Cuts at: 420 861 420
Size: 441 3281
EcoRI G'AATT_C
Cuts at: 726 726
Size: 3722
EcoRV GAT'ATC
Cuts at: 648 648
Size: 3722
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1741 1741
Size: 3722
FspI TGC'GCA
Cuts at: 1503 1503
Size: 3722
HaeII r_GCGC'y
Cuts at: 315 1156 1404 2426 3405 315
Size: 841 248 1022 979 632
Fragments arranged by size:
1022 979 841 632 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 637 669 1564 1594 1596 1626 637
Size: 32 895 30 2 30 2733
Fragments arranged by size:
2733 895 32 30 30 2
HincII GTy'rAC
Cuts at: 761 761
Size: 3722
KasI G'GCGC_C
Cuts at: 1152 1400 1152
Size: 248 3474
KpnI G_GTAC'C
Cuts at: 742 742
Size: 3722
MluI A'CGCG_T
Cuts at: 3155 3155
Size: 3722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 538 2393 2577 538
Size: 1855 184 1683
Fragments arranged by size:
1855 1683 184
MscI TGG'CCA
Cuts at: 478 1483 478
Size: 1005 2717
MslI CAynn'nnrTG
Cuts at: 322 1711 1838 322
Size: 1389 127 2206
Fragments arranged by size:
2206 1389 127
MspA1I CmG'CkG
Cuts at: 288 704 833 1147 1507 2520 2765 288
Size: 416 129 314 360 1013 245 1245
Fragments arranged by size:
1245 1013 416 360 314 245 129
NaeI GCC'GGC
Cuts at: 1903 1903
Size: 3722
NarI GG'CG_CC
Cuts at: 1153 1401 1153
Size: 248 3474
NcoI C'CATG_G
Cuts at: 107 107
Size: 3722
NgoMIV G'CCGG_C
Cuts at: 1901 1901
Size: 3722
NspI r_CATG'y
Cuts at: 775 1275 2182 775
Size: 500 907 2315
Fragments arranged by size:
2315 907 500
PciI A'CATG_T
Cuts at: 2178 2178
Size: 3722
PmeI GTTT'AAAC
Cuts at: 721 721
Size: 3722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2861 2893 2861
Size: 32 3690
PpuMI rG'GwC_Cy
Cuts at: 420 420
Size: 3722
PstI C_TGCA'G
Cuts at: 769 1454 769
Size: 685 3037
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3722
PvuII CAG'CTG
Cuts at: 288 704 1147 1507 288
Size: 416 443 360 2503
Fragments arranged by size:
2503 443 416 360
RsrII CG'GwC_CG
Cuts at: 1917 1917
Size: 3722
SacI G_AGCT'C
Cuts at: 736 736
Size: 3722
SalI G'TCGA_C
Cuts at: 759 759
Size: 3722
SapI GCTCTTCn'nnn_
Cuts at: 1745 1955 1745
Size: 210 3512
SbfI CC_TGCA'GG
Cuts at: 769 769
Size: 3722
ScaI AGT'ACT
Cuts at: 3239 3239
Size: 3722
SfcI C'TryA_G
Cuts at: 33 765 1450 2443 2634 33
Size: 732 685 993 191 1121
Fragments arranged by size:
1121 993 732 685 191
SfoI GGC'GCC
Cuts at: 1154 1402 1154
Size: 248 3474
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3722
SmaI CCC'GGG
Cuts at: 744 744
Size: 3722
SmlI C'TyrA_G
Cuts at: 877 1792 2284 2546 877
Size: 915 492 262 2053
Fragments arranged by size:
2053 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3722
SphI G_CATG'C
Cuts at: 775 1275 775
Size: 500 3222
SspI AAT'ATT
Cuts at: 591 591
Size: 3722
StyI C'CwwG_G
Cuts at: 107 499 666 856 1210 107
Size: 392 167 190 354 2619
Fragments arranged by size:
2619 392 354 190 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 679 1305 1438 1897 2106 679
Size: 626 133 459 209 2295
Fragments arranged by size:
2295 626 459 209 133
TatI w'GTAC_w
Cuts at: 3237 3237
Size: 3722
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 202 234 443 475 3239 3271 202
Size: 32 209 32 2764 32 653
Fragments arranged by size:
2764 653 209 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1519 1519
Size: 3722
XbaI T'CTAG_A
Cuts at: 753 753
Size: 3722
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 228 1217 228
Size: 989 2733
XmaI C'CCGG_G
Cuts at: 742 742
Size: 3722
XmnI GAAnn'nnTTC
Cuts at: 547 547
Size: 3722
ZraI GAC'GTC
Cuts at: 170 170
Size: 3722
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI