Restriction map for
pF1KB5011
(Circular) (Six-base) MAPSORT of: pF1KB5011.seq Check: 5296 from: 1 to: 4364
pF1KB5011 4364 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1020 1020
Size: 4364
AccI GT'mk_AC
Cuts at: 1402 1402
Size: 4364
Acc65I G'GTAC_C
Cuts at: 1380 1380
Size: 4364
AclI AA'CG_TT
Cuts at: 4204 4204
Size: 4364
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2189 2621 3368 3636 2189
Size: 432 747 268 2917
Fragments arranged by size:
2917 747 432 268
AflIII A'CryG_T
Cuts at: 1356 1716 2820 3797 1356
Size: 360 1104 977 1923
Fragments arranged by size:
1923 1104 977 360
AgeI A'CCGG_T
Cuts at: 2752 2752
Size: 4364
AlwNI CAG_nnn'CTG
Cuts at: 288 471 882 1308 1669 3236 288
Size: 183 411 426 361 1567 1416
Fragments arranged by size:
1567 1416 426 411 361 183
ApaLI G'TGCA_C
Cuts at: 3134 3134
Size: 4364
ApoI r'AATT_y
Cuts at: 1368 3717 3973 1368
Size: 2349 256 1759
Fragments arranged by size:
2349 1759 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4364
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4364
AvaI C'yCGr_G
Cuts at: 1384 1384
Size: 4364
BamHI G'GATC_C
Cuts at: 1389 1389
Size: 4364
BanI G'GyrC_C
Cuts at: 1380 1794 2042 2077 1380
Size: 414 248 35 3667
Fragments arranged by size:
3667 414 248 35
BanII G_rGCy'C
Cuts at: 342 1378 2408 342
Size: 1036 1030 2298
Fragments arranged by size:
2298 1036 1030
BbeI G_GCGC'C
Cuts at: 1798 2046 1798
Size: 248 4116
BbsI GAAGACnn'nnnn_
Cuts at: 692 779 1340 692
Size: 87 561 3716
Fragments arranged by size:
3716 561 87
BciVI GTATCCnnnnn_n'
Cuts at: 2262 3023 4364 2262
Size: 761 1341 2262
Fragments arranged by size:
2262 1341 761
BclI T'GATC_A
Cuts at: 1884 1884
Size: 4364
BglI GCCn_nnn'nGGC
Cuts at: 1006 1006
Size: 4364
BglII A'GATC_T
Cuts at: 1879 3916 1879
Size: 2037 2327
BlpI GC'TnA_GC
Cuts at: 214 848 1476 214
Size: 634 628 3102
Fragments arranged by size:
3102 634 628
Bme1580I G_kGCm'C
Cuts at: 1989 2082 3138 1989
Size: 93 1056 3215
Fragments arranged by size:
3215 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1576 1657 1992 4136 1576
Size: 81 335 2144 1804
Fragments arranged by size:
2144 1804 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2731 2731
Size: 4364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 1370 493
Size: 877 3487
Bpu10I CC'TnA_GC
Cuts at: 1294 1294
Size: 4364
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 788 1540 2419 2911 3209 517
Size: 271 752 879 492 298 1672
Fragments arranged by size:
1672 879 752 492 298 271
BsaI GGTCTCn'nnnn_
Cuts at: 4068 4068
Size: 4364
BsaAI yAC'GTr
Cuts at: 1717 1717
Size: 4364
BsaBI GATnn'nnATC
Cuts at: 6 1878 3915 6
Size: 1872 2037 455
Fragments arranged by size:
2037 1872 455
BsaHI Gr'CG_yC
Cuts at: 1795 2043 2745 4241 1795
Size: 248 702 1496 1918
Fragments arranged by size:
1918 1496 702 248
BsaWI w'CCGG_w
Cuts at: 164 1205 1553 2074 2752 3026 3173 3584 164
Size: 1041 348 521 678 274 147 411 944
Fragments arranged by size:
1041 944 678 521 411 348 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1343 1373 1343
Size: 30 4334
BseYI C'CCAG_C
Cuts at: 634 1789 2547 3124 3620 634
Size: 1155 758 577 496 1378
Fragments arranged by size:
1378 1155 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 892 895 892
Size: 3 4361
BsiEI CG_ry'CG
Cuts at: 104 1906 1952 3160 104
Size: 1802 46 1208 1308
Fragments arranged by size:
1802 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 342 1378 2156 2346 3138 342
Size: 1036 778 190 792 1568
Fragments arranged by size:
1568 1036 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 342 1378 1989 2082 2156 2346 2408 3138 342
Size: 1036 611 93 74 190 62 730 1568
Fragments arranged by size:
1568 1036 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 164 1205 3584 164
Size: 1041 2379 944
Fragments arranged by size:
2379 1041 944
BspHI T'CATG_A
Cuts at: 3540 3540
Size: 4364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 809 1020 1414 1628 1930 2311 809
Size: 211 394 214 302 381 2862
Fragments arranged by size:
2862 394 381 302 214 211
BsrBI CCG'CTC
Cuts at: 50 2656 2710 4194 4359 50
Size: 2606 54 1484 165 55
Fragments arranged by size:
2606 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 995 2276 995
Size: 1281 3083
BsrFI r'CCGG_y
Cuts at: 2362 2543 2752 2362
Size: 181 209 3974
Fragments arranged by size:
3974 209 181
BssSI C'ACGA_G
Cuts at: 2635 2993 2635
Size: 358 4006
BstAPI GCAn_nnn'nTGC
Cuts at: 1741 1741
Size: 4364
BstBI TT'CG_AA
Cuts at: 2725 2725
Size: 4364
BstXI CCAn_nnnn'nTGG
Cuts at: 375 726 375
Size: 351 4013
BstYI r'GATC_y
Cuts at: 1 1389 1856 1879 2214 2460 3472 3558 3570
Size: 1388 467 23 335 246 1012 86 12
Cuts at: 3570 3916 1
Size: 346 449
Fragments arranged by size:
1388 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4364
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2314 2495 2658 2314
Size: 181 163 4020
Fragments arranged by size:
4020 181 163
BtsI GCAGTG_nn'
Cuts at: 317 883 904 1052 1076 1607 317
Size: 566 21 148 24 531 3074
Fragments arranged by size:
3074 566 531 148 24 21
DraI TTT'AAA
Cuts at: 1363 1363
Size: 4364
DrdI GACnn_nn'nnGTC
Cuts at: 2070 2928 2070
Size: 858 3506
EaeI y'GGCC_r
Cuts at: 129 869 992 1949 2123 2514 2541 3886 129
Size: 740 123 957 174 391 27 1345 607
Fragments arranged by size:
1345 957 740 607 391 174 123 27
EagI C'GGCC_G
Cuts at: 1949 1949
Size: 4364
EarI CTCTTCn'nnn_
Cuts at: 134 783 1130 2387 2597 134
Size: 649 347 1257 210 1901
Fragments arranged by size:
1901 1257 649 347 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2572 2882 3028 4174 2572
Size: 310 146 1146 2762
Fragments arranged by size:
2762 1146 310 146
EcoICRI GAG'CTC
Cuts at: 340 1376 340
Size: 1036 3328
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 1370 2189 2621 3368 3636 493
Size: 877 819 432 747 268 1221
Fragments arranged by size:
1221 877 819 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 387 1503 387
Size: 1116 3248
EcoRI G'AATT_C
Cuts at: 1368 1368
Size: 4364
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 4364
FspI TGC'GCA
Cuts at: 304 2145 304
Size: 1841 2523
HaeII r_GCGC'y
Cuts at: 289 1798 2046 3068 4047 289
Size: 1509 248 1022 979 606
Fragments arranged by size:
1509 1022 979 606 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 193 225 1200 1232 2206 2236 2238 2268 193
Size: 32 975 32 974 30 2 30 2289
Fragments arranged by size:
2289 975 974 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1403 1403
Size: 4364
KasI G'GCGC_C
Cuts at: 1794 2042 1794
Size: 248 4116
KpnI G_GTAC'C
Cuts at: 1384 1384
Size: 4364
MluI A'CGCG_T
Cuts at: 3797 3797
Size: 4364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 624 3035 3219 624
Size: 2411 184 1769
Fragments arranged by size:
2411 1769 184
MscI TGG'CCA
Cuts at: 131 994 2125 131
Size: 863 1131 2370
Fragments arranged by size:
2370 1131 863
MslI CAynn'nnrTG
Cuts at: 373 565 1096 2353 2480 373
Size: 192 531 1257 127 2257
Fragments arranged by size:
2257 1257 531 192 127
MspA1I CmG'CkG
Cuts at: 274 393 1105 1233 1475 1789 2149 3162 3407
Size: 119 712 128 242 314 360 1013 245
Cuts at: 3407 274
Size: 1231
Fragments arranged by size:
1231 1013 712 360 314 245 242 128
119
NaeI GCC'GGC
Cuts at: 2545 2545
Size: 4364
NarI GG'CG_CC
Cuts at: 1795 2043 1795
Size: 248 4116
NcoI C'CATG_G
Cuts at: 107 107
Size: 4364
NgoMIV G'CCGG_C
Cuts at: 2543 2543
Size: 4364
NspI r_CATG'y
Cuts at: 592 1417 1917 2824 592
Size: 825 500 907 2132
Fragments arranged by size:
2132 907 825 500
PciI A'CATG_T
Cuts at: 2820 2820
Size: 4364
PflMI CCAn_nnn'nTGG
Cuts at: 288 471 288
Size: 183 4181
PmeI GTTT'AAAC
Cuts at: 1363 1363
Size: 4364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3503 3535 3503
Size: 32 4332
PpuMI rG'GwC_Cy
Cuts at: 387 387
Size: 4364
PsiI TTA'TAA
Cuts at: 895 1019 895
Size: 124 4240
PstI C_TGCA'G
Cuts at: 743 1411 2096 743
Size: 668 685 3011
Fragments arranged by size:
3011 685 668
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4364
PvuII CAG'CTG
Cuts at: 274 1105 1233 1789 2149 274
Size: 831 128 556 360 2489
Fragments arranged by size:
2489 831 556 360 128
RsrII CG'GwC_CG
Cuts at: 2559 2559
Size: 4364
SacI G_AGCT'C
Cuts at: 342 1378 342
Size: 1036 3328
SalI G'TCGA_C
Cuts at: 1401 1401
Size: 4364
SapI GCTCTTCn'nnn_
Cuts at: 2387 2597 2387
Size: 210 4154
SbfI CC_TGCA'GG
Cuts at: 1411 1411
Size: 4364
ScaI AGT'ACT
Cuts at: 619 3881 619
Size: 3262 1102
SexAI A'CCwGG_T
Cuts at: 152 152
Size: 4364
SfcI C'TryA_G
Cuts at: 33 739 1407 2092 3085 3276 33
Size: 706 668 685 993 191 1121
Fragments arranged by size:
1121 993 706 685 668 191
SfoI GGC'GCC
Cuts at: 1796 2044 1796
Size: 248 4116
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4364
SmaI CCC'GGG
Cuts at: 1386 1386
Size: 4364
SmlI C'TyrA_G
Cuts at: 496 803 1519 2434 2926 3188 496
Size: 307 716 915 492 262 1672
Fragments arranged by size:
1672 915 716 492 307 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4364
SphI G_CATG'C
Cuts at: 1417 1917 1417
Size: 500 3864
SspI AAT'ATT
Cuts at: 861 861
Size: 4364
StyI C'CwwG_G
Cuts at: 107 126 1498 1852 107
Size: 19 1372 354 2619
Fragments arranged by size:
2619 1372 354 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1947 2080 2539 2748 1947
Size: 133 459 209 3563
Fragments arranged by size:
3563 459 209 133
TatI w'GTAC_w
Cuts at: 617 3879 617
Size: 3262 1102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3881 3913 3881
Size: 32 4332
Tth111I GACn'n_nGTC
Cuts at: 2161 2161
Size: 4364
XbaI T'CTAG_A
Cuts at: 1395 1395
Size: 4364
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 289 631 1002 1859 289
Size: 342 371 857 2794
Fragments arranged by size:
2794 857 371 342
XmaI C'CCGG_G
Cuts at: 1384 1384
Size: 4364
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PshAI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI