Restriction map for
pF1KB4987
(Circular) (Six-base) MAPSORT of: pF1KB4987.seq Check: 7038 from: 1 to: 4526
pF1KB4987 4526 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AatII G_ACGT'C
Cuts at: 1076 1076
Size: 4526
AccI GT'mk_AC
Cuts at: 1564 1564
Size: 4526
Acc65I G'GTAC_C
Cuts at: 1542 1542
Size: 4526
AclI AA'CG_TT
Cuts at: 4366 4366
Size: 4526
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 626 957 2351 2783 3530 3798 368
Size: 258 331 1394 432 747 268 1096
Fragments arranged by size:
1394 1096 747 432 331 268 258
AflIII A'CryG_T
Cuts at: 1298 1878 2982 3959 1298
Size: 580 1104 977 1865
Fragments arranged by size:
1865 1104 977 580
AgeI A'CCGG_T
Cuts at: 1387 2914 1387
Size: 1527 2999
AlwNI CAG_nnn'CTG
Cuts at: 346 926 1831 3398 346
Size: 580 905 1567 1474
Fragments arranged by size:
1567 1474 905 580
ApaI G_GGCC'C
Cuts at: 598 598
Size: 4526
ApaLI G'TGCA_C
Cuts at: 3296 3296
Size: 4526
ApoI r'AATT_y
Cuts at: 1530 3879 4135 1530
Size: 2349 256 1921
Fragments arranged by size:
2349 1921 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4526
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4526
AvaI C'yCGr_G
Cuts at: 1327 1345 1546 1327
Size: 18 201 4307
Fragments arranged by size:
4307 201 18
BamHI G'GATC_C
Cuts at: 1047 1551 1047
Size: 504 4022
BanI G'GyrC_C
Cuts at: 1141 1542 1956 2204 2239 1141
Size: 401 414 248 35 3428
Fragments arranged by size:
3428 414 401 248 35
BanII G_rGCy'C
Cuts at: 125 173 598 841 1397 1540 2570 125
Size: 48 425 243 556 143 1030 2081
Fragments arranged by size:
2081 1030 556 425 243 143 48
BbeI G_GCGC'C
Cuts at: 1145 1960 2208 1145
Size: 815 248 3463
Fragments arranged by size:
3463 815 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4492
BciVI GTATCCnnnnn_n'
Cuts at: 2424 3185 4526 2424
Size: 761 1341 2424
Fragments arranged by size:
2424 1341 761
BclI T'GATC_A
Cuts at: 2046 2046
Size: 4526
BfrBI ATG'CAT
Cuts at: 615 615
Size: 4526
BglI GCCn_nnn'nGGC
Cuts at: 958 958
Size: 4526
BglII A'GATC_T
Cuts at: 992 2041 4078 992
Size: 1049 2037 1440
Fragments arranged by size:
2037 1440 1049
BlpI GC'TnA_GC
Cuts at: 1638 1638
Size: 4526
Bme1580I G_kGCm'C
Cuts at: 598 677 2151 2244 3300 598
Size: 79 1474 93 1056 1824
Fragments arranged by size:
1824 1474 1056 93 79
BmgBI CAC'GTC
Cuts at: 1299 1299
Size: 4526
BmrI ACTGGGnnnn_n'
Cuts at: 1738 1819 2154 4298 1738
Size: 81 335 2144 1966
Fragments arranged by size:
2144 1966 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1399 2893 1399
Size: 1494 3032
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 281 640 1262 157
Size: 124 359 622 3421
Fragments arranged by size:
3421 622 359 124
Bpu10I CC'TnA_GC
Cuts at: 840 840
Size: 4526
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1381 1702 2581 3073 3371 1381
Size: 321 879 492 298 2536
Fragments arranged by size:
2536 879 492 321 298
BsaI GGTCTCn'nnnn_
Cuts at: 4230 4230
Size: 4526
BsaAI yAC'GTr
Cuts at: 1879 1879
Size: 4526
BsaBI GATnn'nnATC
Cuts at: 6 536 2040 4077 6
Size: 530 1504 2037 455
Fragments arranged by size:
2037 1504 530 455
BsaHI Gr'CG_yC
Cuts at: 1073 1142 1957 2205 2907 4403 1073
Size: 69 815 248 702 1496 1196
Fragments arranged by size:
1496 1196 815 702 248 69
BsaWI w'CCGG_w
Cuts at: 1387 1715 2236 2914 3188 3335 3746 1387
Size: 328 521 678 274 147 411 2167
Fragments arranged by size:
2167 678 521 411 328 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1159 1189 1159
Size: 30 4496
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1167 1276 1167
Size: 109 4417
BseYI C'CCAG_C
Cuts at: 306 1128 1951 2709 3286 3782 306
Size: 822 823 758 577 496 1050
Fragments arranged by size:
1050 823 822 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2068 2114 3322 104
Size: 1964 46 1208 1308
Fragments arranged by size:
1964 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 173 841 1268 1397 1442 1540 2318 2508 3300
Size: 668 427 129 45 98 778 190 792
Cuts at: 3300 173
Size: 1399
Fragments arranged by size:
1399 792 778 668 427 190 129 98
45
BsmI GAATG_Cn'
Cuts at: 765 765
Size: 4526
Bsp1286I G_dGCh'C
Cuts at: 125 173 598 677 841 1268 1397 1442 1540
Size: 48 425 79 164 427 129 45 98
Cuts at: 1540 2151 2244 2318 2508 2570 3300 125
Size: 611 93 74 190 62 730 1351
Fragments arranged by size:
1351 730 611 427 425 190 164 129
98 93 79 74 62 48 45
BspEI T'CCGG_A
Cuts at: 3746 3746
Size: 4526
BspHI T'CATG_A
Cuts at: 1487 3702 1487
Size: 2215 2311
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1576 1790 2092 2473 1576
Size: 214 302 381 3629
Fragments arranged by size:
3629 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2818 2872 4356 4521 50
Size: 2768 54 1484 165 55
Fragments arranged by size:
2768 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 617 2438 617
Size: 1821 2705
BsrFI r'CCGG_y
Cuts at: 478 1387 2524 2705 2914 478
Size: 909 1137 181 209 2090
Fragments arranged by size:
2090 1137 909 209 181
BsrGI T'GTAC_A
Cuts at: 581 581
Size: 4526
BssSI C'ACGA_G
Cuts at: 2797 3155 2797
Size: 358 4168
BstAPI GCAn_nnn'nTGC
Cuts at: 1903 1903
Size: 4526
BstBI TT'CG_AA
Cuts at: 2887 2887
Size: 4526
BstEII G'GTnAC_C
Cuts at: 622 622
Size: 4526
BstYI r'GATC_y
Cuts at: 1 992 1047 1551 2018 2041 2376 2622 3634
Size: 991 55 504 467 23 335 246 1012
Cuts at: 3634 3720 3732 4078 1
Size: 86 12 346 449
Fragments arranged by size:
1012 991 504 467 449 346 335 246
86 55 23 12
BtgI C'CryG_G
Cuts at: 266 367 266
Size: 101 4425
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 138 759 2476 2657 2820 138
Size: 621 1717 181 163 1844
Fragments arranged by size:
1844 1717 621 181 163
BtsI GCAGTG_nn'
Cuts at: 393 1769 393
Size: 1376 3150
DraI TTT'AAA
Cuts at: 1525 1525
Size: 4526
DraIII CAC_nnn'GTG
Cuts at: 650 650
Size: 4526
DrdI GACnn_nn'nnGTC
Cuts at: 2232 3090 2232
Size: 858 3668
EaeI y'GGCC_r
Cuts at: 480 1199 2111 2285 2676 2703 4048 480
Size: 719 912 174 391 27 1345 958
Fragments arranged by size:
1345 958 912 719 391 174 27
EagI C'GGCC_G
Cuts at: 2111 2111
Size: 4526
EarI CTCTTCn'nnn_
Cuts at: 344 606 2549 2759 344
Size: 262 1943 210 2111
Fragments arranged by size:
2111 1943 262 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2734 3044 3190 4336 2734
Size: 310 146 1146 2924
Fragments arranged by size:
2924 1146 310 146
EcoICRI GAG'CTC
Cuts at: 171 839 1395 1538 171
Size: 668 556 143 3159
Fragments arranged by size:
3159 668 556 143
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 281 368 626 640 957 1262 2351 2783
Size: 124 87 258 14 317 305 1089 432
Cuts at: 2783 3530 3798 157
Size: 747 268 885
Fragments arranged by size:
1089 885 747 432 317 305 268 258
124 87 14
EcoNI CCTnn'n_nnAGG
Cuts at: 733 733
Size: 4526
EcoO109I rG'GnC_Cy
Cuts at: 179 594 595 720 1665 179
Size: 415 1 125 945 3040
Fragments arranged by size:
3040 945 415 125 1
EcoRI G'AATT_C
Cuts at: 1530 1530
Size: 4526
EcoRV GAT'ATC
Cuts at: 1014 1014
Size: 4526
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1524 2545 1524
Size: 1021 3505
FspI TGC'GCA
Cuts at: 505 2307 505
Size: 1802 2724
HaeII r_GCGC'y
Cuts at: 1145 1960 2208 3230 4209 1145
Size: 815 248 1022 979 1462
Fragments arranged by size:
1462 1022 979 815 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 112 144 1077 1109 1159 1191 1241 1273 2368
Size: 32 933 32 50 32 50 32 1095
Cuts at: 2368 2398 2400 2430 112
Size: 30 2 30 2208
Fragments arranged by size:
2208 1095 933 50 50 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1565 1565
Size: 4526
KasI G'GCGC_C
Cuts at: 1141 1956 2204 1141
Size: 815 248 3463
Fragments arranged by size:
3463 815 248
KpnI G_GTAC'C
Cuts at: 1546 1546
Size: 4526
MluI A'CGCG_T
Cuts at: 3959 3959
Size: 4526
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3197 3381 3197
Size: 184 4342
MscI TGG'CCA
Cuts at: 2287 2287
Size: 4526
MslI CAynn'nnrTG
Cuts at: 688 832 2515 2642 688
Size: 144 1683 127 2572
Fragments arranged by size:
2572 1683 144 127
MspA1I CmG'CkG
Cuts at: 268 1373 1637 1951 2311 3324 3569 268
Size: 1105 264 314 360 1013 245 1225
Fragments arranged by size:
1225 1105 1013 360 314 264 245
NaeI GCC'GGC
Cuts at: 2707 2707
Size: 4526
NarI GG'CG_CC
Cuts at: 1142 1957 2205 1142
Size: 815 248 3463
Fragments arranged by size:
3463 815 248
NcoI C'CATG_G
Cuts at: 367 367
Size: 4526
NgoMIV G'CCGG_C
Cuts at: 2705 2705
Size: 4526
NsiI A_TGCA'T
Cuts at: 617 617
Size: 4526
NspI r_CATG'y
Cuts at: 619 880 1234 1579 2079 2986 619
Size: 261 354 345 500 907 2159
Fragments arranged by size:
2159 907 500 354 345 261
PciI A'CATG_T
Cuts at: 2982 2982
Size: 4526
PfoI T'CCnGG_A
Cuts at: 656 656
Size: 4526
PmeI GTTT'AAAC
Cuts at: 1525 1525
Size: 4526
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3665 3697 3665
Size: 32 4494
PpuMI rG'GwC_Cy
Cuts at: 179 720 179
Size: 541 3985
PspOMI G'GGCC_C
Cuts at: 594 594
Size: 4526
PspXI vC'TCGA_Gb
Cuts at: 1327 1345 1327
Size: 18 4508
PstI C_TGCA'G
Cuts at: 225 1139 1424 1573 2258 225
Size: 914 285 149 685 2493
Fragments arranged by size:
2493 914 685 285 149
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4526
PvuII CAG'CTG
Cuts at: 1951 2311 1951
Size: 360 4166
RsrII CG'GwC_CG
Cuts at: 2721 2721
Size: 4526
SacI G_AGCT'C
Cuts at: 173 841 1397 1540 173
Size: 668 556 143 3159
Fragments arranged by size:
3159 668 556 143
SacII CC_GC'GG
Cuts at: 269 269
Size: 4526
SalI G'TCGA_C
Cuts at: 1563 1563
Size: 4526
SapI GCTCTTCn'nnn_
Cuts at: 2549 2759 2549
Size: 210 4316
SbfI CC_TGCA'GG
Cuts at: 1139 1573 1139
Size: 434 4092
ScaI AGT'ACT
Cuts at: 4043 4043
Size: 4526
SfcI C'TryA_G
Cuts at: 33 221 741 1135 1420 1569 2254 3247 3438
Size: 188 520 394 285 149 685 993 191
Cuts at: 3438 33
Size: 1121
Fragments arranged by size:
1121 993 685 520 394 285 191 188
149
SfoI GGC'GCC
Cuts at: 1143 1958 2206 1143
Size: 815 248 3463
Fragments arranged by size:
3463 815 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4526
SgrAI Cr'CCGG_yG
Cuts at: 1387 1387
Size: 4526
SmaI CCC'GGG
Cuts at: 1548 1548
Size: 4526
SmlI C'TyrA_G
Cuts at: 1327 1345 1396 1681 2596 3088 3350 1327
Size: 18 51 285 915 492 262 2503
Fragments arranged by size:
2503 915 492 285 262 51 18
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4526
SphI G_CATG'C
Cuts at: 1234 1579 2079 1234
Size: 345 500 3681
Fragments arranged by size:
3681 500 345
StuI AGG'CCT
Cuts at: 739 739
Size: 4526
StyI C'CwwG_G
Cuts at: 367 677 764 1217 1660 2014 367
Size: 310 87 453 443 354 2879
Fragments arranged by size:
2879 453 443 354 310 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2109 2242 2701 2910 2109
Size: 133 459 209 3725
Fragments arranged by size:
3725 459 209 133
TatI w'GTAC_w
Cuts at: 375 581 4041 375
Size: 206 3460 860
Fragments arranged by size:
3460 860 206
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 482 514 980 1003 1012 1035 4043 4075 482
Size: 32 466 23 9 23 3008 32 933
Fragments arranged by size:
3008 933 466 32 32 23 23 9
Tth111I GACn'n_nGTC
Cuts at: 2323 2323
Size: 4526
XbaI T'CTAG_A
Cuts at: 1557 1557
Size: 4526
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2021 2021
Size: 4526
XhoI C'TCGA_G
Cuts at: 1327 1345 1327
Size: 18 4508
XmaI C'CCGG_G
Cuts at: 1546 1546
Size: 4526
XmnI GAAnn'nnTTC
Cuts at: 1483 1483
Size: 4526
ZraI GAC'GTC
Cuts at: 1074 1074
Size: 4526
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PfoI PmeI PpiI PpuMI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BmtI BsgI BsiWI BsmBI
BssHII BstXI BstZ17I Bsu36I ClaI CspCI FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI PacI
PasI PflMI PmlI PshAI PsiI PsrI SanDI SexAI
SfiI SnaBI SrfI SspI SwaI