Restriction map for
pF1KB4977
(Circular) (Six-base) MAPSORT of: pF1KB4977.seq Check: 2769 from: 1 to: 4313
pF1KB4977 4313 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 127 1351 127
Size: 1224 3089
Acc65I G'GTAC_C
Cuts at: 1329 1329
Size: 4313
AclI AA'CG_TT
Cuts at: 4153 4153
Size: 4313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2138 2570 2923 3585 2138
Size: 432 353 662 2866
Fragments arranged by size:
2866 662 432 353
AflIII A'CryG_T
Cuts at: 1665 3465 3746 1665
Size: 1800 281 2232
Fragments arranged by size:
2232 1800 281
AleI CACnn'nnGTG
Cuts at: 781 781
Size: 4313
AlwNI CAG_nnn'CTG
Cuts at: 174 1618 3056 174
Size: 1444 1438 1431
Fragments arranged by size:
1444 1438 1431
ApaLI G'TGCA_C
Cuts at: 3151 3151
Size: 4313
ApoI r'AATT_y
Cuts at: 697 1317 3666 3922 697
Size: 620 2349 256 1088
Fragments arranged by size:
2349 1088 620 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4313
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
AvaI C'yCGr_G
Cuts at: 1333 1333
Size: 4313
BamHI G'GATC_C
Cuts at: 1338 1338
Size: 4313
BanI G'GyrC_C
Cuts at: 1329 1743 1991 2026 1329
Size: 414 248 35 3616
Fragments arranged by size:
3616 414 248 35
BanII G_rGCy'C
Cuts at: 1327 2357 1327
Size: 1030 3283
BbeI G_GCGC'C
Cuts at: 1747 1995 1747
Size: 248 4065
BciVI GTATCCnnnnn_n'
Cuts at: 2211 3267 4313 2211
Size: 1056 1046 2211
Fragments arranged by size:
2211 1056 1046
BclI T'GATC_A
Cuts at: 1833 1833
Size: 4313
BglII A'GATC_T
Cuts at: 1828 3865 1828
Size: 2037 2276
BlpI GC'TnA_GC
Cuts at: 175 1425 175
Size: 1250 3063
Bme1580I G_kGCm'C
Cuts at: 812 1938 2031 3155 812
Size: 1126 93 1124 1970
Fragments arranged by size:
1970 1126 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1136 1525 1606 1941 4085 1136
Size: 389 81 335 2144 1364
Fragments arranged by size:
2144 1364 389 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 605 2680 605
Size: 2075 2238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 230
Size: 4313
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 1489 2368 3082 3380 355
Size: 1134 879 714 298 1288
Fragments arranged by size:
1288 1134 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4017 4017
Size: 4313
BsaAI yAC'GTr
Cuts at: 522 1666 522
Size: 1144 3169
BsaBI GATnn'nnATC
Cuts at: 6 716 839 947 1827 3864 6
Size: 710 123 108 880 2037 455
Fragments arranged by size:
2037 880 710 455 123 108
BsaHI Gr'CG_yC
Cuts at: 1744 1992 2694 4190 1744
Size: 248 702 1496 1867
Fragments arranged by size:
1867 1496 702 248
BsaWI w'CCGG_w
Cuts at: 503 1502 2023 2701 3112 3259 3533 503
Size: 999 521 678 411 147 274 1283
Fragments arranged by size:
1283 999 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1738 2496 3161 3569 1738
Size: 758 665 408 2482
Fragments arranged by size:
2482 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1855 1901 3131 104
Size: 1751 46 1230 1286
Fragments arranged by size:
1751 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1327 2105 2295 3155 1327
Size: 778 190 860 2485
Fragments arranged by size:
2485 860 778 190
BsmI GAATG_Cn'
Cuts at: 927 927
Size: 4313
BsmBI CGTCTCn'nnnn_
Cuts at: 206 206
Size: 4313
Bsp1286I G_dGCh'C
Cuts at: 812 1327 1938 2031 2105 2295 2357 3155 812
Size: 515 611 93 74 190 62 798 1970
Fragments arranged by size:
1970 798 611 515 190 93 74 62
BspHI T'CATG_A
Cuts at: 2745 2745
Size: 4313
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1363 1577 1879 2260 1363
Size: 214 302 381 3416
Fragments arranged by size:
3416 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2605 2659 4143 4308 50
Size: 2555 54 1484 165 55
Fragments arranged by size:
2555 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2225 2225
Size: 4313
BsrFI r'CCGG_y
Cuts at: 135 2311 2492 135
Size: 2176 181 1956
Fragments arranged by size:
2176 1956 181
BssSI C'ACGA_G
Cuts at: 2584 3292 2584
Size: 708 3605
BstAPI GCAn_nnn'nTGC
Cuts at: 1233 1690 1233
Size: 457 3856
BstBI TT'CG_AA
Cuts at: 2674 2674
Size: 4313
BstYI r'GATC_y
Cuts at: 1 432 607 711 1290 1338 1805 1828 2163
Size: 431 175 104 579 48 467 23 335
Cuts at: 2163 2409 2715 2727 2813 3865 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 579 467 449 431 335 306 246
175 104 86 48 23 12
BtgI C'CryG_G
Cuts at: 672 672
Size: 4313
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1059 2263 2444 2607 1059
Size: 1204 181 163 2765
Fragments arranged by size:
2765 1204 181 163
BtsI GCAGTG_nn'
Cuts at: 186 655 1556 186
Size: 469 901 2943
Fragments arranged by size:
2943 901 469
DraI TTT'AAA
Cuts at: 1312 1312
Size: 4313
DrdI GACnn_nn'nnGTC
Cuts at: 2019 3363 2019
Size: 1344 2969
EaeI y'GGCC_r
Cuts at: 158 669 1898 2072 2463 2490 3835 158
Size: 511 1229 174 391 27 1345 636
Fragments arranged by size:
1345 1229 636 511 391 174 27
EagI C'GGCC_G
Cuts at: 1898 1898
Size: 4313
EarI CTCTTCn'nnn_
Cuts at: 2336 2546 2336
Size: 210 4103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2521 3263 3409 4123 2521
Size: 742 146 714 2711
Fragments arranged by size:
2711 742 714 146
EcoICRI GAG'CTC
Cuts at: 1325 1325
Size: 4313
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 2138 2570 2923 3585 230
Size: 1908 432 353 662 958
Fragments arranged by size:
1908 958 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1452 1452
Size: 4313
EcoRI G'AATT_C
Cuts at: 1317 1317
Size: 4313
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2332 2332
Size: 4313
FspI TGC'GCA
Cuts at: 2094 2094
Size: 4313
HaeII r_GCGC'y
Cuts at: 1747 1995 3225 3996 1747
Size: 248 1230 771 2064
Fragments arranged by size:
2064 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 618 650 762 794 2155 2185 2187 2217 618
Size: 32 112 32 1361 30 2 30 2714
Fragments arranged by size:
2714 1361 112 32 32 30 30 2
HincII GTy'rAC
Cuts at: 128 1352 128
Size: 1224 3089
KasI G'GCGC_C
Cuts at: 1743 1991 1743
Size: 248 4065
KpnI G_GTAC'C
Cuts at: 1333 1333
Size: 4313
MluI A'CGCG_T
Cuts at: 3746 3746
Size: 4313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3072 3256 3072
Size: 184 4129
MscI TGG'CCA
Cuts at: 671 2074 671
Size: 1403 2910
MslI CAynn'nnrTG
Cuts at: 677 781 815 2302 2429 677
Size: 104 34 1487 127 2561
Fragments arranged by size:
2561 1487 127 104 34
MspA1I CmG'CkG
Cuts at: 1424 1738 2098 2882 3127 1424
Size: 314 360 784 245 2610
Fragments arranged by size:
2610 784 360 314 245
NaeI GCC'GGC
Cuts at: 137 2494 137
Size: 2357 1956
NarI GG'CG_CC
Cuts at: 1744 1992 1744
Size: 248 4065
NcoI C'CATG_G
Cuts at: 672 672
Size: 4313
NgoMIV G'CCGG_C
Cuts at: 135 2492 135
Size: 2357 1956
NspI r_CATG'y
Cuts at: 153 1366 1866 3469 153
Size: 1213 500 1603 997
Fragments arranged by size:
1603 1213 997 500
PciI A'CATG_T
Cuts at: 3465 3465
Size: 4313
PflMI CCAn_nnn'nTGG
Cuts at: 397 397
Size: 4313
PmeI GTTT'AAAC
Cuts at: 1312 1312
Size: 4313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 806 838 2759 2791 806
Size: 32 1921 32 2328
Fragments arranged by size:
2328 1921 32 32
PsiI TTA'TAA
Cuts at: 947 947
Size: 4313
PstI C_TGCA'G
Cuts at: 1360 2045 1360
Size: 685 3628
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4313
PvuII CAG'CTG
Cuts at: 1738 2098 1738
Size: 360 3953
RsrII CG'GwC_CG
Cuts at: 2508 2508
Size: 4313
SacI G_AGCT'C
Cuts at: 1327 1327
Size: 4313
SalI G'TCGA_C
Cuts at: 126 1350 126
Size: 1224 3089
SapI GCTCTTCn'nnn_
Cuts at: 2336 2546 2336
Size: 210 4103
SbfI CC_TGCA'GG
Cuts at: 1360 1360
Size: 4313
ScaI AGT'ACT
Cuts at: 3830 3830
Size: 4313
SfcI C'TryA_G
Cuts at: 33 452 599 1356 2041 3009 3200 33
Size: 419 147 757 685 968 191 1146
Fragments arranged by size:
1146 968 757 685 419 191 147
SfoI GGC'GCC
Cuts at: 1745 1993 1745
Size: 248 4065
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
SgrAI Cr'CCGG_yG
Cuts at: 135 135
Size: 4313
SmaI CCC'GGG
Cuts at: 1335 1335
Size: 4313
SmlI C'TyrA_G
Cuts at: 370 1468 2383 3097 3359 370
Size: 1098 915 714 262 1324
Fragments arranged by size:
1324 1098 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4313
SphI G_CATG'C
Cuts at: 1366 1866 1366
Size: 500 3813
SspI AAT'ATT
Cuts at: 346 791 346
Size: 445 3868
StyI C'CwwG_G
Cuts at: 672 1304 1447 1801 672
Size: 632 143 354 3184
Fragments arranged by size:
3184 632 354 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 838 1896 2029 2488 2697 838
Size: 1058 133 459 209 2454
Fragments arranged by size:
2454 1058 459 209 133
TatI w'GTAC_w
Cuts at: 3828 3828
Size: 4313
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3830 3862 3830
Size: 32 4281
Tth111I GACn'n_nGTC
Cuts at: 229 2110 229
Size: 1881 2432
XbaI T'CTAG_A
Cuts at: 610 1344 610
Size: 734 3579
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 394 1808 394
Size: 1414 2899
XmaI C'CCGG_G
Cuts at: 1333 1333
Size: 4313
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI
BspEI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI